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Q9R0G7

- ZEB2_MOUSE

UniProt

Q9R0G7 - ZEB2_MOUSE

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Protein
Zinc finger E-box-binding homeobox 2
Gene
Zeb2, Sip1, Zfhx1b, Zfx1b
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri211 – 23424C2H2-type 1
Add
BLAST
Zinc fingeri241 – 26323C2H2-type 2
Add
BLAST
Zinc fingeri282 – 30423C2H2-type 3
Add
BLAST
Zinc fingeri310 – 33425C2H2-type 4; atypical
Add
BLAST
DNA bindingi644 – 70360Homeobox; atypical
Add
BLAST
Zinc fingeri999 – 102123C2H2-type 5
Add
BLAST
Zinc fingeri1027 – 104923C2H2-type 6
Add
BLAST
Zinc fingeri1055 – 107622C2H2-type 7; atypical
Add
BLAST

GO - Molecular functioni

  1. R-SMAD binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. sequence-specific DNA binding Source: InterPro
  4. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. cell proliferation in forebrain Source: MGI
  2. central nervous system development Source: MGI
  3. developmental pigmentation Source: BHF-UCL
  4. embryonic morphogenesis Source: MGI
  5. hippocampus development Source: MGI
  6. melanocyte migration Source: BHF-UCL
  7. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  8. neural crest cell migration Source: MGI
  9. neural tube closure Source: MGI
  10. positive regulation of JUN kinase activity Source: MGI
  11. positive regulation of Wnt signaling pathway Source: MGI
  12. positive regulation of melanin biosynthetic process Source: BHF-UCL
  13. positive regulation of melanocyte differentiation Source: BHF-UCL
  14. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  15. regulation of melanosome organization Source: BHF-UCL
  16. somitogenesis Source: MGI
  17. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger E-box-binding homeobox 2
Alternative name(s):
Smad-interacting protein 1
Zinc finger homeobox protein 1b
Gene namesi
Name:Zeb2
Synonyms:Sip1, Zfhx1b, Zfx1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1344407. Zeb2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleolus Source: Ensembl
  3. nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12151215Zinc finger E-box-binding homeobox 2
PRO_0000047237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei377 – 3771N6-acetyllysine By similarity
Cross-linki391 – 391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-linki866 – 866Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Post-translational modificationi

Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity By similarity.

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ9R0G7.
PaxDbiQ9R0G7.
PRIDEiQ9R0G7.

PTM databases

PhosphoSiteiQ9R0G7.

Expressioni

Gene expression databases

ArrayExpressiQ9R0G7.
BgeeiQ9R0G7.
CleanExiMM_ZEB2.
GenevestigatoriQ9R0G7.

Interactioni

Subunit structurei

Interacts with CBX4 and CTBP1 By similarity. Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected.

Protein-protein interaction databases

BioGridi204915. 3 interactions.
IntActiQ9R0G7. 1 interaction.
MINTiMINT-99176.
STRINGi10090.ENSMUSP00000028229.

Structurei

3D structure databases

ProteinModelPortaliQ9R0G7.
SMRiQ9R0G7. Positions 184-359, 568-606, 648-705, 945-1081.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni437 – 48751SMAD-MH2 binding domain
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1084 – 1215132Glu-rich (acidic)
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00630000089829.
HOVERGENiHBG004697.
InParanoidiQ6P9M5.
KOiK09299.
OMAiEKLDHSR.
OrthoDBiEOG790G0D.
TreeFamiTF331759.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 8 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0G7-1 [UniParc]FASTAAdd to Basket

« Hide

MKQPIMADGP RCKRRKQANP RRKNVVNYDN VVDAGSETDE EDKLHIAEDD     50
SLANPLDQDT SPASMPNHES SPHMSQGLLP REEEEEELRE SVVEHSWHSG 100
EILQASVAGP EEMKEDYDAM GPEATIQTTI NNGTVKNANC TSDFEEYFAK 150
RKLEERDGHA VSIEEYLQRS DTAIIYPEAP EELSRLGTPE ANGQEENDLP 200
PGTPDAFAQL LTCPYCDRGY KRLTSLKEHI KYRHEKNEEN FSCPLCSYTF 250
AYRTQLERHM VTHKPGTDQH QMLTQGAGNR KFKCTECGKA FKYKHHLKEH 300
LRIHSGEKPY ECPNCKKRFS HSGSYSSHIS SKKCIGLISV NGRMRNNIKT 350
GSSPNSVSSS PTNSAITQLR NKLENGKPLS MSEQTGLLKI KTEPLDFNDY 400
KVLMATHGFS GSSPFMNGGL GATSPLGVHP SAQSPMQHLG VGMEAPLLGF 450
PTMNSNLSEV QKVLQIVDNT VSRQKMDCKT EDISKLKGYH MKDPCSQPEE 500
QGVTSPNIPP VGLPVVSHNG ATKSIIDYTL EKVNEAKACL QSLTTDSRRQ 550
ISNIKKEKLR TLIDLVTDDK MIENHSISTP FSCQFCKESF PGPIPLHQHE 600
RYLCKMNEEI KAVLQPHENI VPNKAGVFVD NKALLLSSVL SEKGLTSPIN 650
PYKDHMSVLK AYYAMNMEPN SDELLKISIA VGLPQEFVKE WFEQRKVYQY 700
SNSRSPSLER TSKPLAPNSN PTTKDSLLPR SPVKPMDSIT SPSIAELHNS 750
VTSCDPPLRL TKSSHFTNIK AVDKLDHSRS NTPSPLNLSS TSSKNSHSSS 800
YTPNSFSSEE LQAEPLDLSL PKQMREPKGI IATKNKTKAT SINLDHNSVS 850
SSSENSDEPL NLTFIKKEFS NSNNLDNKSN NPVFGMNPFS AKPLYTPLPP 900
QSAFPPATFM PPVQTSIPGL RPYPGLDQMS FLPHMAYTYP TGAATFADMQ 950
QRRKYQRKQG FQGDLLDGAQ DYMSGLDDMT DSDSCLSRKK IKKTESGMYA 1000
CDLCDKTFQK SSSLLRHKYE HTGKRPHQCQ ICKKAFKHKH HLIEHSRLHS 1050
GEKPYQCDKC GKRFSHSGSY SQHMNHRYSY CKREAEEREA AEREAREKGH 1100
LEPTELLMNR AYLQSITPQG YSDSEERESM PRDGESEKEH EKEGEEGYGK 1150
LRRRDGDEEE EEEEEESENK SMDTDPETIR DEEETGDHSM DDSSEDGKME 1200
TKSDHEEDNM EDGME 1215
Length:1,215
Mass (Da):136,615
Last modified:July 27, 2011 - v2
Checksum:i1DA4236E43A96BE7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1102 – 11021E → G in AAD56590. 1 Publication
Sequence conflicti1215 – 12151E → G in AAD56590. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF033116 mRNA. Translation: AAD56590.1.
AL935127, AL929120 Genomic DNA. Translation: CAM24133.1.
AL929120, AL935127 Genomic DNA. Translation: CAM24896.1.
BC060699 mRNA. Translation: AAH60699.1.
CCDSiCCDS16020.1.
RefSeqiNP_001276450.1. NM_001289521.1.
NP_056568.2. NM_015753.4.
XP_006498108.1. XM_006498045.1.
XP_006498109.1. XM_006498046.1.
XP_006498110.1. XM_006498047.1.
UniGeneiMm.440702.

Genome annotation databases

EnsembliENSMUST00000028229; ENSMUSP00000028229; ENSMUSG00000026872.
ENSMUST00000068415; ENSMUSP00000069685; ENSMUSG00000026872.
ENSMUST00000176438; ENSMUSP00000134849; ENSMUSG00000026872.
ENSMUST00000177302; ENSMUSP00000134747; ENSMUSG00000026872.
GeneIDi24136.
KEGGimmu:24136.
UCSCiuc008jpc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF033116 mRNA. Translation: AAD56590.1 .
AL935127 , AL929120 Genomic DNA. Translation: CAM24133.1 .
AL929120 , AL935127 Genomic DNA. Translation: CAM24896.1 .
BC060699 mRNA. Translation: AAH60699.1 .
CCDSi CCDS16020.1.
RefSeqi NP_001276450.1. NM_001289521.1.
NP_056568.2. NM_015753.4.
XP_006498108.1. XM_006498045.1.
XP_006498109.1. XM_006498046.1.
XP_006498110.1. XM_006498047.1.
UniGenei Mm.440702.

3D structure databases

ProteinModelPortali Q9R0G7.
SMRi Q9R0G7. Positions 184-359, 568-606, 648-705, 945-1081.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204915. 3 interactions.
IntActi Q9R0G7. 1 interaction.
MINTi MINT-99176.
STRINGi 10090.ENSMUSP00000028229.

PTM databases

PhosphoSitei Q9R0G7.

Proteomic databases

MaxQBi Q9R0G7.
PaxDbi Q9R0G7.
PRIDEi Q9R0G7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028229 ; ENSMUSP00000028229 ; ENSMUSG00000026872 .
ENSMUST00000068415 ; ENSMUSP00000069685 ; ENSMUSG00000026872 .
ENSMUST00000176438 ; ENSMUSP00000134849 ; ENSMUSG00000026872 .
ENSMUST00000177302 ; ENSMUSP00000134747 ; ENSMUSG00000026872 .
GeneIDi 24136.
KEGGi mmu:24136.
UCSCi uc008jpc.1. mouse.

Organism-specific databases

CTDi 9839.
MGIi MGI:1344407. Zeb2.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00630000089829.
HOVERGENi HBG004697.
InParanoidi Q6P9M5.
KOi K09299.
OMAi EKLDHSR.
OrthoDBi EOG790G0D.
TreeFami TF331759.

Miscellaneous databases

ChiTaRSi ZEB2. mouse.
NextBioi 304195.
PROi Q9R0G7.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9R0G7.
Bgeei Q9R0G7.
CleanExi MM_ZEB2.
Genevestigatori Q9R0G7.

Family and domain databases

Gene3Di 3.30.160.60. 5 hits.
InterProi IPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF05605. zf-Di19. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 8 hits.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5'-CACCT sequences in candidate target genes."
    Verschueren K., Remacle J.E., Collart C., Kraft H., Baker B.S., Tylzanowski P., Nelles L., Wuytens G., Su M.-T., Bodmer R., Smith J.C., Huylebroeck D.
    J. Biol. Chem. 274:20489-20498(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiZEB2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0G7
Secondary accession number(s): Q6P9M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi