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Protein

Zinc finger E-box-binding homeobox 2

Gene

Zeb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri211 – 234C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri241 – 263C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri282 – 304C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri310 – 334C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST25
Zinc fingeri581 – 605C2H2-type 5; degeneratePROSITE-ProRule annotationAdd BLAST25
DNA bindingi644 – 703Homeobox; atypicalAdd BLAST60
Zinc fingeri999 – 1021C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1027 – 1049C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1055 – 1076C2H2-type 8; atypicalPROSITE-ProRule annotationAdd BLAST22

GO - Molecular functioni

GO - Biological processi

  • cell proliferation in forebrain Source: MGI
  • central nervous system development Source: MGI
  • collateral sprouting Source: MGI
  • corpus callosum morphogenesis Source: MGI
  • corticospinal tract morphogenesis Source: MGI
  • developmental pigmentation Source: BHF-UCL
  • embryonic morphogenesis Source: MGI
  • hippocampus development Source: MGI
  • mammillary axonal complex development Source: MGI
  • melanocyte migration Source: BHF-UCL
  • negative regulation of transcription by RNA polymerase II Source: BHF-UCL
  • neural crest cell migration Source: MGI
  • neural tube closure Source: MGI
  • positive regulation of axonogenesis Source: MGI
  • positive regulation of JUN kinase activity Source: MGI
  • positive regulation of lens fiber cell differentiation Source: UniProtKB
  • positive regulation of melanin biosynthetic process Source: BHF-UCL
  • positive regulation of melanocyte differentiation Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of Wnt signaling pathway Source: MGI
  • regulation of melanosome organization Source: BHF-UCL
  • somitogenesis Source: MGI

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger E-box-binding homeobox 2
Alternative name(s):
Smad-interacting protein 1
Zinc finger homeobox protein 1b
Gene namesi
Name:Zeb2
Synonyms:Sip1, Zfhx1b, Zfx1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1344407 Zeb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000472371 – 1215Zinc finger E-box-binding homeobox 2Add BLAST1215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei142PhosphoserineBy similarity1
Modified residuei356PhosphoserineCombined sources1
Modified residuei360PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei377N6-acetyllysineBy similarity1
Cross-linki391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki555Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki611Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki632Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei647PhosphoserineBy similarity1
Cross-linki713Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei731PhosphoserineCombined sources1
Modified residuei780PhosphoserineCombined sources1
Modified residuei782PhosphothreonineBy similarity1
Modified residuei784PhosphoserineCombined sources1
Cross-linki866Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki866Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei1122PhosphoserineBy similarity1
Modified residuei1124PhosphoserineCombined sources1
Modified residuei1203PhosphoserineCombined sources1

Post-translational modificationi

Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9R0G7
PaxDbiQ9R0G7
PRIDEiQ9R0G7

PTM databases

iPTMnetiQ9R0G7
PhosphoSitePlusiQ9R0G7
SwissPalmiQ9R0G7

Expressioni

Gene expression databases

BgeeiENSMUSG00000026872
CleanExiMM_ZEB2
ExpressionAtlasiQ9R0G7 baseline and differential
GenevisibleiQ9R0G7 MM

Interactioni

Subunit structurei

Interacts with CBX4 and CTBP1 (By similarity). Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected.By similarity

GO - Molecular functioni

  • R-SMAD binding Source: MGI

Protein-protein interaction databases

BioGridi204915, 4 interactors
IntActiQ9R0G7, 2 interactors
MINTiQ9R0G7
STRINGi10090.ENSMUSP00000028229

Structurei

3D structure databases

ProteinModelPortaliQ9R0G7
SMRiQ9R0G7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni437 – 487SMAD-MH2 binding domainAdd BLAST51

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1084 – 1215Glu-rich (acidic)Add BLAST132

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri211 – 234C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri241 – 263C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri282 – 304C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri310 – 334C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST25
Zinc fingeri581 – 605C2H2-type 5; degeneratePROSITE-ProRule annotationAdd BLAST25
Zinc fingeri999 – 1021C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1027 – 1049C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1055 – 1076C2H2-type 8; atypicalPROSITE-ProRule annotationAdd BLAST22

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KCPI Eukaryota
ENOG410ZQ40 LUCA
GeneTreeiENSGT00630000089829
HOVERGENiHBG004697
InParanoidiQ9R0G7
KOiK09299
OrthoDBiEOG091G0F7Y
TreeFamiTF331759

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR008598 Di19_Zn_binding_dom
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF05605 zf-Di19, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00355 ZnF_C2H2, 8 hits
SUPFAMiSSF46689 SSF46689, 1 hit
SSF57667 SSF57667, 4 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 5 hits
PS50157 ZINC_FINGER_C2H2_2, 6 hits

Sequencei

Sequence statusi: Complete.

Q9R0G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQPIMADGP RCKRRKQANP RRKNVVNYDN VVDAGSETDE EDKLHIAEDD
60 70 80 90 100
SLANPLDQDT SPASMPNHES SPHMSQGLLP REEEEEELRE SVVEHSWHSG
110 120 130 140 150
EILQASVAGP EEMKEDYDAM GPEATIQTTI NNGTVKNANC TSDFEEYFAK
160 170 180 190 200
RKLEERDGHA VSIEEYLQRS DTAIIYPEAP EELSRLGTPE ANGQEENDLP
210 220 230 240 250
PGTPDAFAQL LTCPYCDRGY KRLTSLKEHI KYRHEKNEEN FSCPLCSYTF
260 270 280 290 300
AYRTQLERHM VTHKPGTDQH QMLTQGAGNR KFKCTECGKA FKYKHHLKEH
310 320 330 340 350
LRIHSGEKPY ECPNCKKRFS HSGSYSSHIS SKKCIGLISV NGRMRNNIKT
360 370 380 390 400
GSSPNSVSSS PTNSAITQLR NKLENGKPLS MSEQTGLLKI KTEPLDFNDY
410 420 430 440 450
KVLMATHGFS GSSPFMNGGL GATSPLGVHP SAQSPMQHLG VGMEAPLLGF
460 470 480 490 500
PTMNSNLSEV QKVLQIVDNT VSRQKMDCKT EDISKLKGYH MKDPCSQPEE
510 520 530 540 550
QGVTSPNIPP VGLPVVSHNG ATKSIIDYTL EKVNEAKACL QSLTTDSRRQ
560 570 580 590 600
ISNIKKEKLR TLIDLVTDDK MIENHSISTP FSCQFCKESF PGPIPLHQHE
610 620 630 640 650
RYLCKMNEEI KAVLQPHENI VPNKAGVFVD NKALLLSSVL SEKGLTSPIN
660 670 680 690 700
PYKDHMSVLK AYYAMNMEPN SDELLKISIA VGLPQEFVKE WFEQRKVYQY
710 720 730 740 750
SNSRSPSLER TSKPLAPNSN PTTKDSLLPR SPVKPMDSIT SPSIAELHNS
760 770 780 790 800
VTSCDPPLRL TKSSHFTNIK AVDKLDHSRS NTPSPLNLSS TSSKNSHSSS
810 820 830 840 850
YTPNSFSSEE LQAEPLDLSL PKQMREPKGI IATKNKTKAT SINLDHNSVS
860 870 880 890 900
SSSENSDEPL NLTFIKKEFS NSNNLDNKSN NPVFGMNPFS AKPLYTPLPP
910 920 930 940 950
QSAFPPATFM PPVQTSIPGL RPYPGLDQMS FLPHMAYTYP TGAATFADMQ
960 970 980 990 1000
QRRKYQRKQG FQGDLLDGAQ DYMSGLDDMT DSDSCLSRKK IKKTESGMYA
1010 1020 1030 1040 1050
CDLCDKTFQK SSSLLRHKYE HTGKRPHQCQ ICKKAFKHKH HLIEHSRLHS
1060 1070 1080 1090 1100
GEKPYQCDKC GKRFSHSGSY SQHMNHRYSY CKREAEEREA AEREAREKGH
1110 1120 1130 1140 1150
LEPTELLMNR AYLQSITPQG YSDSEERESM PRDGESEKEH EKEGEEGYGK
1160 1170 1180 1190 1200
LRRRDGDEEE EEEEEESENK SMDTDPETIR DEEETGDHSM DDSSEDGKME
1210
TKSDHEEDNM EDGME
Length:1,215
Mass (Da):136,615
Last modified:July 27, 2011 - v2
Checksum:i1DA4236E43A96BE7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1102E → G in AAD56590 (PubMed:10400677).Curated1
Sequence conflicti1215E → G in AAD56590 (PubMed:10400677).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033116 mRNA Translation: AAD56590.1
AL935127, AL929120 Genomic DNA Translation: CAM24133.1
AL929120, AL935127 Genomic DNA Translation: CAM24896.1
BC060699 mRNA Translation: AAH60699.1
CCDSiCCDS16020.1
RefSeqiNP_001276450.1, NM_001289521.1
NP_056568.2, NM_015753.4
XP_011237404.1, XM_011239102.2
XP_017173550.1, XM_017318061.1
XP_017173552.1, XM_017318063.1
XP_017173554.1, XM_017318065.1
UniGeneiMm.440702

Genome annotation databases

EnsembliENSMUST00000068415; ENSMUSP00000069685; ENSMUSG00000026872
ENSMUST00000176438; ENSMUSP00000134849; ENSMUSG00000026872
ENSMUST00000177302; ENSMUSP00000134747; ENSMUSG00000026872
GeneIDi24136
KEGGimmu:24136
UCSCiuc008jpc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiZEB2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0G7
Secondary accession number(s): Q6P9M5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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