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Protein

Zinc finger E-box-binding homeobox 2

Gene

Zeb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri211 – 23424C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri241 – 26323C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri282 – 30423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri310 – 33425C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
DNA bindingi644 – 70360Homeobox; atypicalAdd
BLAST
Zinc fingeri999 – 102123C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1027 – 104923C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1055 – 107622C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. R-SMAD binding Source: MGI

GO - Biological processi

  1. cell proliferation in forebrain Source: MGI
  2. central nervous system development Source: MGI
  3. developmental pigmentation Source: BHF-UCL
  4. embryonic morphogenesis Source: MGI
  5. hippocampus development Source: MGI
  6. melanocyte migration Source: BHF-UCL
  7. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  8. neural crest cell migration Source: MGI
  9. neural tube closure Source: MGI
  10. positive regulation of JUN kinase activity Source: MGI
  11. positive regulation of melanin biosynthetic process Source: BHF-UCL
  12. positive regulation of melanocyte differentiation Source: BHF-UCL
  13. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  14. positive regulation of Wnt signaling pathway Source: MGI
  15. regulation of melanosome organization Source: BHF-UCL
  16. somitogenesis Source: MGI
  17. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger E-box-binding homeobox 2
Alternative name(s):
Smad-interacting protein 1
Zinc finger homeobox protein 1b
Gene namesi
Name:Zeb2
Synonyms:Sip1, Zfhx1b, Zfx1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1344407. Zeb2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12151215Zinc finger E-box-binding homeobox 2PRO_0000047237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei377 – 3771N6-acetyllysineBy similarity
Cross-linki391 – 391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki866 – 866Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ9R0G7.
PaxDbiQ9R0G7.
PRIDEiQ9R0G7.

PTM databases

PhosphoSiteiQ9R0G7.

Expressioni

Gene expression databases

BgeeiQ9R0G7.
CleanExiMM_ZEB2.
ExpressionAtlasiQ9R0G7. baseline and differential.
GenevestigatoriQ9R0G7.

Interactioni

Subunit structurei

Interacts with CBX4 and CTBP1 (By similarity). Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected.By similarity

Protein-protein interaction databases

BioGridi204915. 3 interactions.
IntActiQ9R0G7. 1 interaction.
MINTiMINT-99176.
STRINGi10090.ENSMUSP00000028229.

Structurei

3D structure databases

ProteinModelPortaliQ9R0G7.
SMRiQ9R0G7. Positions 202-353, 648-705, 999-1073.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni437 – 48751SMAD-MH2 binding domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1084 – 1215132Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri211 – 23424C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri241 – 26323C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri282 – 30423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri310 – 33425C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri999 – 102123C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1027 – 104923C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1055 – 107622C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00630000089829.
HOVERGENiHBG004697.
InParanoidiQ9R0G7.
KOiK09299.
OMAiLDHSRSN.
OrthoDBiEOG790G0D.
TreeFamiTF331759.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 8 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQPIMADGP RCKRRKQANP RRKNVVNYDN VVDAGSETDE EDKLHIAEDD
60 70 80 90 100
SLANPLDQDT SPASMPNHES SPHMSQGLLP REEEEEELRE SVVEHSWHSG
110 120 130 140 150
EILQASVAGP EEMKEDYDAM GPEATIQTTI NNGTVKNANC TSDFEEYFAK
160 170 180 190 200
RKLEERDGHA VSIEEYLQRS DTAIIYPEAP EELSRLGTPE ANGQEENDLP
210 220 230 240 250
PGTPDAFAQL LTCPYCDRGY KRLTSLKEHI KYRHEKNEEN FSCPLCSYTF
260 270 280 290 300
AYRTQLERHM VTHKPGTDQH QMLTQGAGNR KFKCTECGKA FKYKHHLKEH
310 320 330 340 350
LRIHSGEKPY ECPNCKKRFS HSGSYSSHIS SKKCIGLISV NGRMRNNIKT
360 370 380 390 400
GSSPNSVSSS PTNSAITQLR NKLENGKPLS MSEQTGLLKI KTEPLDFNDY
410 420 430 440 450
KVLMATHGFS GSSPFMNGGL GATSPLGVHP SAQSPMQHLG VGMEAPLLGF
460 470 480 490 500
PTMNSNLSEV QKVLQIVDNT VSRQKMDCKT EDISKLKGYH MKDPCSQPEE
510 520 530 540 550
QGVTSPNIPP VGLPVVSHNG ATKSIIDYTL EKVNEAKACL QSLTTDSRRQ
560 570 580 590 600
ISNIKKEKLR TLIDLVTDDK MIENHSISTP FSCQFCKESF PGPIPLHQHE
610 620 630 640 650
RYLCKMNEEI KAVLQPHENI VPNKAGVFVD NKALLLSSVL SEKGLTSPIN
660 670 680 690 700
PYKDHMSVLK AYYAMNMEPN SDELLKISIA VGLPQEFVKE WFEQRKVYQY
710 720 730 740 750
SNSRSPSLER TSKPLAPNSN PTTKDSLLPR SPVKPMDSIT SPSIAELHNS
760 770 780 790 800
VTSCDPPLRL TKSSHFTNIK AVDKLDHSRS NTPSPLNLSS TSSKNSHSSS
810 820 830 840 850
YTPNSFSSEE LQAEPLDLSL PKQMREPKGI IATKNKTKAT SINLDHNSVS
860 870 880 890 900
SSSENSDEPL NLTFIKKEFS NSNNLDNKSN NPVFGMNPFS AKPLYTPLPP
910 920 930 940 950
QSAFPPATFM PPVQTSIPGL RPYPGLDQMS FLPHMAYTYP TGAATFADMQ
960 970 980 990 1000
QRRKYQRKQG FQGDLLDGAQ DYMSGLDDMT DSDSCLSRKK IKKTESGMYA
1010 1020 1030 1040 1050
CDLCDKTFQK SSSLLRHKYE HTGKRPHQCQ ICKKAFKHKH HLIEHSRLHS
1060 1070 1080 1090 1100
GEKPYQCDKC GKRFSHSGSY SQHMNHRYSY CKREAEEREA AEREAREKGH
1110 1120 1130 1140 1150
LEPTELLMNR AYLQSITPQG YSDSEERESM PRDGESEKEH EKEGEEGYGK
1160 1170 1180 1190 1200
LRRRDGDEEE EEEEEESENK SMDTDPETIR DEEETGDHSM DDSSEDGKME
1210
TKSDHEEDNM EDGME
Length:1,215
Mass (Da):136,615
Last modified:July 27, 2011 - v2
Checksum:i1DA4236E43A96BE7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1102 – 11021E → G in AAD56590 (PubMed:10400677).Curated
Sequence conflicti1215 – 12151E → G in AAD56590 (PubMed:10400677).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033116 mRNA. Translation: AAD56590.1.
AL935127, AL929120 Genomic DNA. Translation: CAM24133.1.
AL929120, AL935127 Genomic DNA. Translation: CAM24896.1.
BC060699 mRNA. Translation: AAH60699.1.
CCDSiCCDS16020.1.
RefSeqiNP_001276450.1. NM_001289521.1.
NP_056568.2. NM_015753.4.
XP_006498108.1. XM_006498045.1.
XP_006498109.1. XM_006498046.1.
XP_006498110.1. XM_006498047.1.
UniGeneiMm.440702.

Genome annotation databases

EnsembliENSMUST00000028229; ENSMUSP00000028229; ENSMUSG00000026872.
ENSMUST00000068415; ENSMUSP00000069685; ENSMUSG00000026872.
ENSMUST00000176438; ENSMUSP00000134849; ENSMUSG00000026872.
ENSMUST00000177302; ENSMUSP00000134747; ENSMUSG00000026872.
GeneIDi24136.
KEGGimmu:24136.
UCSCiuc008jpc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033116 mRNA. Translation: AAD56590.1.
AL935127, AL929120 Genomic DNA. Translation: CAM24133.1.
AL929120, AL935127 Genomic DNA. Translation: CAM24896.1.
BC060699 mRNA. Translation: AAH60699.1.
CCDSiCCDS16020.1.
RefSeqiNP_001276450.1. NM_001289521.1.
NP_056568.2. NM_015753.4.
XP_006498108.1. XM_006498045.1.
XP_006498109.1. XM_006498046.1.
XP_006498110.1. XM_006498047.1.
UniGeneiMm.440702.

3D structure databases

ProteinModelPortaliQ9R0G7.
SMRiQ9R0G7. Positions 202-353, 648-705, 999-1073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204915. 3 interactions.
IntActiQ9R0G7. 1 interaction.
MINTiMINT-99176.
STRINGi10090.ENSMUSP00000028229.

PTM databases

PhosphoSiteiQ9R0G7.

Proteomic databases

MaxQBiQ9R0G7.
PaxDbiQ9R0G7.
PRIDEiQ9R0G7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028229; ENSMUSP00000028229; ENSMUSG00000026872.
ENSMUST00000068415; ENSMUSP00000069685; ENSMUSG00000026872.
ENSMUST00000176438; ENSMUSP00000134849; ENSMUSG00000026872.
ENSMUST00000177302; ENSMUSP00000134747; ENSMUSG00000026872.
GeneIDi24136.
KEGGimmu:24136.
UCSCiuc008jpc.1. mouse.

Organism-specific databases

CTDi9839.
MGIiMGI:1344407. Zeb2.

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00630000089829.
HOVERGENiHBG004697.
InParanoidiQ9R0G7.
KOiK09299.
OMAiLDHSRSN.
OrthoDBiEOG790G0D.
TreeFamiTF331759.

Miscellaneous databases

ChiTaRSiZeb2. mouse.
NextBioi304195.
PROiQ9R0G7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0G7.
CleanExiMM_ZEB2.
ExpressionAtlasiQ9R0G7. baseline and differential.
GenevestigatoriQ9R0G7.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 8 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5'-CACCT sequences in candidate target genes."
    Verschueren K., Remacle J.E., Collart C., Kraft H., Baker B.S., Tylzanowski P., Nelles L., Wuytens G., Su M.-T., Bodmer R., Smith J.C., Huylebroeck D.
    J. Biol. Chem. 274:20489-20498(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiZEB2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0G7
Secondary accession number(s): Q6P9M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.