Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9R0G7 (ZEB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger E-box-binding homeobox 2
Alternative name(s):
Smad-interacting protein 1
Zinc finger homeobox protein 1b
Gene names
Name:Zeb2
Synonyms:Sip1, Zfhx1b, Zfx1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1215 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

Subunit structure

Interacts with CBX4 and CTBP1 By similarity. Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected.

Subcellular location

Nucleus.

Post-translational modification

Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity By similarity.

Sequence similarities

Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Contains 7 C2H2-type zinc fingers.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
Repeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   PTMAcetylation
Isopeptide bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation in forebrain

Inferred from mutant phenotype PubMed 17644613. Source: MGI

central nervous system development

Inferred from genetic interaction PubMed 16598713. Source: MGI

embryonic morphogenesis

Inferred from genetic interaction PubMed 16598713. Source: MGI

hippocampus development

Inferred from mutant phenotype PubMed 17644613. Source: MGI

neural crest cell migration

Inferred from mutant phenotype PubMed 12522767. Source: MGI

neural tube closure

Inferred from mutant phenotype PubMed 16598713. Source: MGI

positive regulation of JUN kinase activity

Inferred from mutant phenotype PubMed 17644613. Source: MGI

positive regulation of Wnt signaling pathway

Inferred from mutant phenotype PubMed 17644613. Source: MGI

somitogenesis

Inferred from mutant phenotype PubMed 16157277PubMed 16598713. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleolus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionR-SMAD binding

Inferred from direct assay PubMed 16115198. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12151215Zinc finger E-box-binding homeobox 2
PRO_0000047237

Regions

Zinc finger211 – 23424C2H2-type 1
Zinc finger241 – 26323C2H2-type 2
Zinc finger282 – 30423C2H2-type 3
Zinc finger310 – 33425C2H2-type 4; atypical
DNA binding644 – 70360Homeobox; atypical
Zinc finger999 – 102123C2H2-type 5
Zinc finger1027 – 104923C2H2-type 6
Zinc finger1055 – 107622C2H2-type 7; atypical
Region437 – 48751SMAD-MH2 binding domain
Compositional bias1084 – 1215132Glu-rich (acidic)

Amino acid modifications

Modified residue3771N6-acetyllysine By similarity
Cross-link391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link866Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Experimental info

Sequence conflict11021E → G in AAD56590. Ref.1
Sequence conflict12151E → G in AAD56590. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9R0G7 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 1DA4236E43A96BE7

FASTA1,215136,615
        10         20         30         40         50         60 
MKQPIMADGP RCKRRKQANP RRKNVVNYDN VVDAGSETDE EDKLHIAEDD SLANPLDQDT 

        70         80         90        100        110        120 
SPASMPNHES SPHMSQGLLP REEEEEELRE SVVEHSWHSG EILQASVAGP EEMKEDYDAM 

       130        140        150        160        170        180 
GPEATIQTTI NNGTVKNANC TSDFEEYFAK RKLEERDGHA VSIEEYLQRS DTAIIYPEAP 

       190        200        210        220        230        240 
EELSRLGTPE ANGQEENDLP PGTPDAFAQL LTCPYCDRGY KRLTSLKEHI KYRHEKNEEN 

       250        260        270        280        290        300 
FSCPLCSYTF AYRTQLERHM VTHKPGTDQH QMLTQGAGNR KFKCTECGKA FKYKHHLKEH 

       310        320        330        340        350        360 
LRIHSGEKPY ECPNCKKRFS HSGSYSSHIS SKKCIGLISV NGRMRNNIKT GSSPNSVSSS 

       370        380        390        400        410        420 
PTNSAITQLR NKLENGKPLS MSEQTGLLKI KTEPLDFNDY KVLMATHGFS GSSPFMNGGL 

       430        440        450        460        470        480 
GATSPLGVHP SAQSPMQHLG VGMEAPLLGF PTMNSNLSEV QKVLQIVDNT VSRQKMDCKT 

       490        500        510        520        530        540 
EDISKLKGYH MKDPCSQPEE QGVTSPNIPP VGLPVVSHNG ATKSIIDYTL EKVNEAKACL 

       550        560        570        580        590        600 
QSLTTDSRRQ ISNIKKEKLR TLIDLVTDDK MIENHSISTP FSCQFCKESF PGPIPLHQHE 

       610        620        630        640        650        660 
RYLCKMNEEI KAVLQPHENI VPNKAGVFVD NKALLLSSVL SEKGLTSPIN PYKDHMSVLK 

       670        680        690        700        710        720 
AYYAMNMEPN SDELLKISIA VGLPQEFVKE WFEQRKVYQY SNSRSPSLER TSKPLAPNSN 

       730        740        750        760        770        780 
PTTKDSLLPR SPVKPMDSIT SPSIAELHNS VTSCDPPLRL TKSSHFTNIK AVDKLDHSRS 

       790        800        810        820        830        840 
NTPSPLNLSS TSSKNSHSSS YTPNSFSSEE LQAEPLDLSL PKQMREPKGI IATKNKTKAT 

       850        860        870        880        890        900 
SINLDHNSVS SSSENSDEPL NLTFIKKEFS NSNNLDNKSN NPVFGMNPFS AKPLYTPLPP 

       910        920        930        940        950        960 
QSAFPPATFM PPVQTSIPGL RPYPGLDQMS FLPHMAYTYP TGAATFADMQ QRRKYQRKQG 

       970        980        990       1000       1010       1020 
FQGDLLDGAQ DYMSGLDDMT DSDSCLSRKK IKKTESGMYA CDLCDKTFQK SSSLLRHKYE 

      1030       1040       1050       1060       1070       1080 
HTGKRPHQCQ ICKKAFKHKH HLIEHSRLHS GEKPYQCDKC GKRFSHSGSY SQHMNHRYSY 

      1090       1100       1110       1120       1130       1140 
CKREAEEREA AEREAREKGH LEPTELLMNR AYLQSITPQG YSDSEERESM PRDGESEKEH 

      1150       1160       1170       1180       1190       1200 
EKEGEEGYGK LRRRDGDEEE EEEEEESENK SMDTDPETIR DEEETGDHSM DDSSEDGKME 

      1210 
TKSDHEEDNM EDGME 

« Hide

References

« Hide 'large scale' references
[1]"SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5'-CACCT sequences in candidate target genes."
Verschueren K., Remacle J.E., Collart C., Kraft H., Baker B.S., Tylzanowski P., Nelles L., Wuytens G., Su M.-T., Bodmer R., Smith J.C., Huylebroeck D.
J. Biol. Chem. 274:20489-20498(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF033116 mRNA. Translation: AAD56590.1.
AL935127, AL929120 Genomic DNA. Translation: CAM24133.1.
AL929120, AL935127 Genomic DNA. Translation: CAM24896.1.
BC060699 mRNA. Translation: AAH60699.1.
CCDSCCDS16020.1.
RefSeqNP_001276450.1. NM_001289521.1.
NP_056568.2. NM_015753.4.
XP_006498108.1. XM_006498045.1.
XP_006498109.1. XM_006498046.1.
XP_006498110.1. XM_006498047.1.
UniGeneMm.440702.

3D structure databases

ProteinModelPortalQ9R0G7.
SMRQ9R0G7. Positions 184-359, 568-606, 648-705, 945-1081.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204915. 3 interactions.
IntActQ9R0G7. 1 interaction.
MINTMINT-99176.
STRING10090.ENSMUSP00000028229.

PTM databases

PhosphoSiteQ9R0G7.

Proteomic databases

MaxQBQ9R0G7.
PaxDbQ9R0G7.
PRIDEQ9R0G7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028229; ENSMUSP00000028229; ENSMUSG00000026872.
ENSMUST00000068415; ENSMUSP00000069685; ENSMUSG00000026872.
ENSMUST00000176438; ENSMUSP00000134849; ENSMUSG00000026872.
ENSMUST00000177302; ENSMUSP00000134747; ENSMUSG00000026872.
GeneID24136.
KEGGmmu:24136.
UCSCuc008jpc.1. mouse.

Organism-specific databases

CTD9839.
MGIMGI:1344407. Zeb2.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00630000089829.
HOVERGENHBG004697.
InParanoidQ6P9M5.
KOK09299.
OMAEKLDHSR.
OrthoDBEOG790G0D.
TreeFamTF331759.

Gene expression databases

ArrayExpressQ9R0G7.
BgeeQ9R0G7.
CleanExMM_ZEB2.
GenevestigatorQ9R0G7.

Family and domain databases

Gene3D3.30.160.60. 5 hits.
InterProIPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 8 hits.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZEB2. mouse.
NextBio304195.
PROQ9R0G7.
SOURCESearch...

Entry information

Entry nameZEB2_MOUSE
AccessionPrimary (citable) accession number: Q9R0G7
Secondary accession number(s): Q6P9M5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot