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Q9R0G7

- ZEB2_MOUSE

UniProt

Q9R0G7 - ZEB2_MOUSE

Protein

Zinc finger E-box-binding homeobox 2

Gene

Zeb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri211 – 23424C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri241 – 26323C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri282 – 30423C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri310 – 33425C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi644 – 70360Homeobox; atypicalAdd
    BLAST
    Zinc fingeri999 – 102123C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1027 – 104923C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1055 – 107622C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. R-SMAD binding Source: MGI
    3. sequence-specific DNA binding Source: InterPro
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. cell proliferation in forebrain Source: MGI
    2. central nervous system development Source: MGI
    3. developmental pigmentation Source: BHF-UCL
    4. embryonic morphogenesis Source: MGI
    5. hippocampus development Source: MGI
    6. melanocyte migration Source: BHF-UCL
    7. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    8. neural crest cell migration Source: MGI
    9. neural tube closure Source: MGI
    10. positive regulation of JUN kinase activity Source: MGI
    11. positive regulation of melanin biosynthetic process Source: BHF-UCL
    12. positive regulation of melanocyte differentiation Source: BHF-UCL
    13. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    14. positive regulation of Wnt signaling pathway Source: MGI
    15. regulation of melanosome organization Source: BHF-UCL
    16. somitogenesis Source: MGI
    17. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger E-box-binding homeobox 2
    Alternative name(s):
    Smad-interacting protein 1
    Zinc finger homeobox protein 1b
    Gene namesi
    Name:Zeb2
    Synonyms:Sip1, Zfhx1b, Zfx1b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1344407. Zeb2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleolus Source: Ensembl
    3. nucleus Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12151215Zinc finger E-box-binding homeobox 2PRO_0000047237Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei377 – 3771N6-acetyllysineBy similarity
    Cross-linki391 – 391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Cross-linki866 – 866Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

    Post-translational modificationi

    Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiQ9R0G7.
    PaxDbiQ9R0G7.
    PRIDEiQ9R0G7.

    PTM databases

    PhosphoSiteiQ9R0G7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9R0G7.
    BgeeiQ9R0G7.
    CleanExiMM_ZEB2.
    GenevestigatoriQ9R0G7.

    Interactioni

    Subunit structurei

    Interacts with CBX4 and CTBP1 By similarity. Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected.By similarity

    Protein-protein interaction databases

    BioGridi204915. 3 interactions.
    IntActiQ9R0G7. 1 interaction.
    MINTiMINT-99176.
    STRINGi10090.ENSMUSP00000028229.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0G7.
    SMRiQ9R0G7. Positions 184-359, 568-606, 648-705, 945-1081.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni437 – 48751SMAD-MH2 binding domainAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1084 – 1215132Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 homeobox DNA-binding domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri211 – 23424C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri241 – 26323C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri282 – 30423C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri310 – 33425C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri999 – 102123C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1027 – 104923C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1055 – 107622C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Homeobox, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00630000089829.
    HOVERGENiHBG004697.
    InParanoidiQ6P9M5.
    KOiK09299.
    OMAiEKLDHSR.
    OrthoDBiEOG790G0D.
    TreeFamiTF331759.

    Family and domain databases

    Gene3Di3.30.160.60. 5 hits.
    InterProiIPR008598. Di19_Zn_binding.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF05605. zf-Di19. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 8 hits.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
    PS50157. ZINC_FINGER_C2H2_2. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R0G7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQPIMADGP RCKRRKQANP RRKNVVNYDN VVDAGSETDE EDKLHIAEDD     50
    SLANPLDQDT SPASMPNHES SPHMSQGLLP REEEEEELRE SVVEHSWHSG 100
    EILQASVAGP EEMKEDYDAM GPEATIQTTI NNGTVKNANC TSDFEEYFAK 150
    RKLEERDGHA VSIEEYLQRS DTAIIYPEAP EELSRLGTPE ANGQEENDLP 200
    PGTPDAFAQL LTCPYCDRGY KRLTSLKEHI KYRHEKNEEN FSCPLCSYTF 250
    AYRTQLERHM VTHKPGTDQH QMLTQGAGNR KFKCTECGKA FKYKHHLKEH 300
    LRIHSGEKPY ECPNCKKRFS HSGSYSSHIS SKKCIGLISV NGRMRNNIKT 350
    GSSPNSVSSS PTNSAITQLR NKLENGKPLS MSEQTGLLKI KTEPLDFNDY 400
    KVLMATHGFS GSSPFMNGGL GATSPLGVHP SAQSPMQHLG VGMEAPLLGF 450
    PTMNSNLSEV QKVLQIVDNT VSRQKMDCKT EDISKLKGYH MKDPCSQPEE 500
    QGVTSPNIPP VGLPVVSHNG ATKSIIDYTL EKVNEAKACL QSLTTDSRRQ 550
    ISNIKKEKLR TLIDLVTDDK MIENHSISTP FSCQFCKESF PGPIPLHQHE 600
    RYLCKMNEEI KAVLQPHENI VPNKAGVFVD NKALLLSSVL SEKGLTSPIN 650
    PYKDHMSVLK AYYAMNMEPN SDELLKISIA VGLPQEFVKE WFEQRKVYQY 700
    SNSRSPSLER TSKPLAPNSN PTTKDSLLPR SPVKPMDSIT SPSIAELHNS 750
    VTSCDPPLRL TKSSHFTNIK AVDKLDHSRS NTPSPLNLSS TSSKNSHSSS 800
    YTPNSFSSEE LQAEPLDLSL PKQMREPKGI IATKNKTKAT SINLDHNSVS 850
    SSSENSDEPL NLTFIKKEFS NSNNLDNKSN NPVFGMNPFS AKPLYTPLPP 900
    QSAFPPATFM PPVQTSIPGL RPYPGLDQMS FLPHMAYTYP TGAATFADMQ 950
    QRRKYQRKQG FQGDLLDGAQ DYMSGLDDMT DSDSCLSRKK IKKTESGMYA 1000
    CDLCDKTFQK SSSLLRHKYE HTGKRPHQCQ ICKKAFKHKH HLIEHSRLHS 1050
    GEKPYQCDKC GKRFSHSGSY SQHMNHRYSY CKREAEEREA AEREAREKGH 1100
    LEPTELLMNR AYLQSITPQG YSDSEERESM PRDGESEKEH EKEGEEGYGK 1150
    LRRRDGDEEE EEEEEESENK SMDTDPETIR DEEETGDHSM DDSSEDGKME 1200
    TKSDHEEDNM EDGME 1215
    Length:1,215
    Mass (Da):136,615
    Last modified:July 27, 2011 - v2
    Checksum:i1DA4236E43A96BE7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1102 – 11021E → G in AAD56590. (PubMed:10400677)Curated
    Sequence conflicti1215 – 12151E → G in AAD56590. (PubMed:10400677)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033116 mRNA. Translation: AAD56590.1.
    AL935127, AL929120 Genomic DNA. Translation: CAM24133.1.
    AL929120, AL935127 Genomic DNA. Translation: CAM24896.1.
    BC060699 mRNA. Translation: AAH60699.1.
    CCDSiCCDS16020.1.
    RefSeqiNP_001276450.1. NM_001289521.1.
    NP_056568.2. NM_015753.4.
    XP_006498108.1. XM_006498045.1.
    XP_006498109.1. XM_006498046.1.
    XP_006498110.1. XM_006498047.1.
    UniGeneiMm.440702.

    Genome annotation databases

    EnsembliENSMUST00000028229; ENSMUSP00000028229; ENSMUSG00000026872.
    ENSMUST00000068415; ENSMUSP00000069685; ENSMUSG00000026872.
    ENSMUST00000176438; ENSMUSP00000134849; ENSMUSG00000026872.
    ENSMUST00000177302; ENSMUSP00000134747; ENSMUSG00000026872.
    GeneIDi24136.
    KEGGimmu:24136.
    UCSCiuc008jpc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033116 mRNA. Translation: AAD56590.1 .
    AL935127 , AL929120 Genomic DNA. Translation: CAM24133.1 .
    AL929120 , AL935127 Genomic DNA. Translation: CAM24896.1 .
    BC060699 mRNA. Translation: AAH60699.1 .
    CCDSi CCDS16020.1.
    RefSeqi NP_001276450.1. NM_001289521.1.
    NP_056568.2. NM_015753.4.
    XP_006498108.1. XM_006498045.1.
    XP_006498109.1. XM_006498046.1.
    XP_006498110.1. XM_006498047.1.
    UniGenei Mm.440702.

    3D structure databases

    ProteinModelPortali Q9R0G7.
    SMRi Q9R0G7. Positions 184-359, 568-606, 648-705, 945-1081.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204915. 3 interactions.
    IntActi Q9R0G7. 1 interaction.
    MINTi MINT-99176.
    STRINGi 10090.ENSMUSP00000028229.

    PTM databases

    PhosphoSitei Q9R0G7.

    Proteomic databases

    MaxQBi Q9R0G7.
    PaxDbi Q9R0G7.
    PRIDEi Q9R0G7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028229 ; ENSMUSP00000028229 ; ENSMUSG00000026872 .
    ENSMUST00000068415 ; ENSMUSP00000069685 ; ENSMUSG00000026872 .
    ENSMUST00000176438 ; ENSMUSP00000134849 ; ENSMUSG00000026872 .
    ENSMUST00000177302 ; ENSMUSP00000134747 ; ENSMUSG00000026872 .
    GeneIDi 24136.
    KEGGi mmu:24136.
    UCSCi uc008jpc.1. mouse.

    Organism-specific databases

    CTDi 9839.
    MGIi MGI:1344407. Zeb2.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00630000089829.
    HOVERGENi HBG004697.
    InParanoidi Q6P9M5.
    KOi K09299.
    OMAi EKLDHSR.
    OrthoDBi EOG790G0D.
    TreeFami TF331759.

    Miscellaneous databases

    ChiTaRSi ZEB2. mouse.
    NextBioi 304195.
    PROi Q9R0G7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R0G7.
    Bgeei Q9R0G7.
    CleanExi MM_ZEB2.
    Genevestigatori Q9R0G7.

    Family and domain databases

    Gene3Di 3.30.160.60. 5 hits.
    InterProi IPR008598. Di19_Zn_binding.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF05605. zf-Di19. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 8 hits.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 5 hits.
    PS50157. ZINC_FINGER_C2H2_2. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5'-CACCT sequences in candidate target genes."
      Verschueren K., Remacle J.E., Collart C., Kraft H., Baker B.S., Tylzanowski P., Nelles L., Wuytens G., Su M.-T., Bodmer R., Smith J.C., Huylebroeck D.
      J. Biol. Chem. 274:20489-20498(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiZEB2_MOUSE
    AccessioniPrimary (citable) accession number: Q9R0G7
    Secondary accession number(s): Q6P9M5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3