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Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene

Plod1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (By similarity). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (PubMed:27119146).By similarity1 Publication

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Fe2+By similarity
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi657IronPROSITE-ProRule annotation1
Metal bindingi659IronPROSITE-ProRule annotation1
Metal bindingi709IronPROSITE-ProRule annotation1
Active sitei719Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.4 3474
ReactomeiR-MMU-1650814 Collagen biosynthesis and modifying enzymes

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (EC:1.14.11.4By similarity)
Alternative name(s):
Lysyl hydroxylase 1
Short name:
LH1
Gene namesi
Name:Plod1
Synonyms:Plod
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:99907 Plod1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000002467919 – 728Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1Add BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi198N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi539N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi687N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9R0E2
PaxDbiQ9R0E2
PeptideAtlasiQ9R0E2
PRIDEiQ9R0E2

PTM databases

iPTMnetiQ9R0E2
PhosphoSitePlusiQ9R0E2

Expressioni

Tissue specificityi

Highly expressed in the liver, heart, lung, skeletal muscle and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000019055
ExpressionAtlasiQ9R0E2 baseline and differential
GenevisibleiQ9R0E2 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Identified in a complex with P3H3 and P3H4 (PubMed:27119146).By similarity1 Publication

Protein-protein interaction databases

BioGridi202253, 1 interactor
IntActiQ9R0E2, 1 interactor
MINTiQ9R0E2
STRINGi10090.ENSMUSP00000019199

Structurei

3D structure databases

ProteinModelPortaliQ9R0E2
SMRiQ9R0E2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini637 – 728Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST92

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971 Eukaryota
ENOG410Y4QU LUCA
GeneTreeiENSGT00550000074427
HOGENOMiHOG000231099
HOVERGENiHBG053618
InParanoidiQ9R0E2
KOiK00473
OMAiKFEMGHV
OrthoDBiEOG091G02DK
PhylomeDBiQ9R0E2
TreeFamiTF313826

Family and domain databases

InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR006620 Pro_4_hyd_alph
IPR001006 Procol_lys_dOase
PfamiView protein in Pfam
PF13640 2OG-FeII_Oxy_3, 1 hit
SMARTiView protein in SMART
SM00702 P4Hc, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit
PS01325 LYS_HYDROXYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLLLLAPL AWLLLVQAKD DAKLEDNLLV LTVATKETEG FRRFKRSAQF
60 70 80 90 100
FNYKIQSLGL GEDWSVDGGP AAAGGGQKVR LLKKALEKHA DKEDLVILFV
110 120 130 140 150
DSYDVVFASG PRELLKKFQQ AKSQVVFSAE EHIYPDRRLE AKYPTVPDGK
160 170 180 190 200
RFLGSGGFIG YAPSLSKLVA EWEGQDSDSD QLFYTKIFLN PEKREQINIS
210 220 230 240 250
LDHRCRIFQN LDGALDEVVL KFEMGHVRAR NLAYDTLPVV VHGNGPTKLQ
260 270 280 290 300
LNYLGNYIPR FWTFETGCTV CDEGLRSLKG IGDEALPTVL VGVFIEQPTP
310 320 330 340 350
FLSLFFLRLL RLRYPQKQMR LFIHNQERHH KLQVEQFLAE HGSEYQSVKL
360 370 380 390 400
VGPEVRMANA DARNMGADLC RQDQTCTYYF SVDADVALTE PNSLRLLIEQ
410 420 430 440 450
NKNVIAPLMT RHGRLWSNFW GGLSADGYYA RSEDYVDIVQ GRRVGVWNVP
460 470 480 490 500
YISNIYLIKG SALRAELQNV DLFHYSKLDS DMSFCANVRQ QEVFMFLTNR
510 520 530 540 550
HTFGHLLSLD NYQTTHLHND LWEVFSNPED WKEKYIHENY TKALAGKLVE
560 570 580 590 600
TPCPDVYWFP IFTEAACDEL VEEMEHYGQW SLGDNKDNRI QGGYENVPTI
610 620 630 640 650
DIHMNQITFE REWHKFLVEY IAPMTEKLYP GYYTRAQFDL AFVVRYKPDE
660 670 680 690 700
QPSLMPHHDA STFTVNIALN RVGEDYEGGG CRFLRYNCSV RAPRKGWALL
710 720
HPGRLTHYHE GLPTTKGTRY IAVSFVDP
Length:728
Mass (Da):83,595
Last modified:May 1, 2000 - v1
Checksum:i339C2D71976ED985
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046782 mRNA Translation: AAD54617.1
BC006599 mRNA Translation: AAH06599.1
CCDSiCCDS18924.1
RefSeqiNP_035252.1, NM_011122.3
UniGeneiMm.23951
Mm.37371

Genome annotation databases

EnsembliENSMUST00000019199; ENSMUSP00000019199; ENSMUSG00000019055
GeneIDi18822
KEGGimmu:18822
UCSCiuc008vtk.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPLOD1_MOUSE
AccessioniPrimary (citable) accession number: Q9R0E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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