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Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene

Plod1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi657 – 6571IronPROSITE-ProRule annotation
Metal bindingi659 – 6591IronPROSITE-ProRule annotation
Metal bindingi709 – 7091IronPROSITE-ProRule annotation
Active sitei719 – 7191Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.4. 3474.
ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (EC:1.14.11.4)
Alternative name(s):
Lysyl hydroxylase 1
Short name:
LH1
Gene namesi
Name:Plod1
Synonyms:Plod
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:99907. Plod1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 728710Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1PRO_0000024679Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi198 – 1981N-linked (GlcNAc...)1 Publication
Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence analysis
Glycosylationi687 – 6871N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9R0E2.
PaxDbiQ9R0E2.
PRIDEiQ9R0E2.

PTM databases

PhosphoSiteiQ9R0E2.

Expressioni

Tissue specificityi

Highly expressed in the liver, heart, lung, skeletal muscle and kidney.1 Publication

Gene expression databases

BgeeiQ9R0E2.
ExpressionAtlasiQ9R0E2. baseline and differential.
GenevisibleiQ9R0E2. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi202253. 1 interaction.
IntActiQ9R0E2. 1 interaction.
MINTiMINT-4107945.
STRINGi10090.ENSMUSP00000019199.

Structurei

3D structure databases

ProteinModelPortaliQ9R0E2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini637 – 72892Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971. Eukaryota.
ENOG410Y4QU. LUCA.
GeneTreeiENSGT00550000074427.
HOGENOMiHOG000231099.
HOVERGENiHBG053618.
InParanoidiQ9R0E2.
KOiK00473.
OMAiKFEMGHV.
OrthoDBiEOG79PJNP.
PhylomeDBiQ9R0E2.
TreeFamiTF313826.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLLLLAPL AWLLLVQAKD DAKLEDNLLV LTVATKETEG FRRFKRSAQF
60 70 80 90 100
FNYKIQSLGL GEDWSVDGGP AAAGGGQKVR LLKKALEKHA DKEDLVILFV
110 120 130 140 150
DSYDVVFASG PRELLKKFQQ AKSQVVFSAE EHIYPDRRLE AKYPTVPDGK
160 170 180 190 200
RFLGSGGFIG YAPSLSKLVA EWEGQDSDSD QLFYTKIFLN PEKREQINIS
210 220 230 240 250
LDHRCRIFQN LDGALDEVVL KFEMGHVRAR NLAYDTLPVV VHGNGPTKLQ
260 270 280 290 300
LNYLGNYIPR FWTFETGCTV CDEGLRSLKG IGDEALPTVL VGVFIEQPTP
310 320 330 340 350
FLSLFFLRLL RLRYPQKQMR LFIHNQERHH KLQVEQFLAE HGSEYQSVKL
360 370 380 390 400
VGPEVRMANA DARNMGADLC RQDQTCTYYF SVDADVALTE PNSLRLLIEQ
410 420 430 440 450
NKNVIAPLMT RHGRLWSNFW GGLSADGYYA RSEDYVDIVQ GRRVGVWNVP
460 470 480 490 500
YISNIYLIKG SALRAELQNV DLFHYSKLDS DMSFCANVRQ QEVFMFLTNR
510 520 530 540 550
HTFGHLLSLD NYQTTHLHND LWEVFSNPED WKEKYIHENY TKALAGKLVE
560 570 580 590 600
TPCPDVYWFP IFTEAACDEL VEEMEHYGQW SLGDNKDNRI QGGYENVPTI
610 620 630 640 650
DIHMNQITFE REWHKFLVEY IAPMTEKLYP GYYTRAQFDL AFVVRYKPDE
660 670 680 690 700
QPSLMPHHDA STFTVNIALN RVGEDYEGGG CRFLRYNCSV RAPRKGWALL
710 720
HPGRLTHYHE GLPTTKGTRY IAVSFVDP
Length:728
Mass (Da):83,595
Last modified:May 1, 2000 - v1
Checksum:i339C2D71976ED985
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046782 mRNA. Translation: AAD54617.1.
BC006599 mRNA. Translation: AAH06599.1.
CCDSiCCDS18924.1.
RefSeqiNP_035252.1. NM_011122.3.
UniGeneiMm.23951.
Mm.37371.

Genome annotation databases

EnsembliENSMUST00000019199; ENSMUSP00000019199; ENSMUSG00000019055.
GeneIDi18822.
KEGGimmu:18822.
UCSCiuc008vtk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046782 mRNA. Translation: AAD54617.1.
BC006599 mRNA. Translation: AAH06599.1.
CCDSiCCDS18924.1.
RefSeqiNP_035252.1. NM_011122.3.
UniGeneiMm.23951.
Mm.37371.

3D structure databases

ProteinModelPortaliQ9R0E2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202253. 1 interaction.
IntActiQ9R0E2. 1 interaction.
MINTiMINT-4107945.
STRINGi10090.ENSMUSP00000019199.

PTM databases

PhosphoSiteiQ9R0E2.

Proteomic databases

MaxQBiQ9R0E2.
PaxDbiQ9R0E2.
PRIDEiQ9R0E2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019199; ENSMUSP00000019199; ENSMUSG00000019055.
GeneIDi18822.
KEGGimmu:18822.
UCSCiuc008vtk.2. mouse.

Organism-specific databases

CTDi5351.
MGIiMGI:99907. Plod1.

Phylogenomic databases

eggNOGiKOG1971. Eukaryota.
ENOG410Y4QU. LUCA.
GeneTreeiENSGT00550000074427.
HOGENOMiHOG000231099.
HOVERGENiHBG053618.
InParanoidiQ9R0E2.
KOiK00473.
OMAiKFEMGHV.
OrthoDBiEOG79PJNP.
PhylomeDBiQ9R0E2.
TreeFamiTF313826.

Enzyme and pathway databases

BRENDAi1.14.11.4. 3474.
ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

ChiTaRSiPlod1. mouse.
NextBioi295184.
PROiQ9R0E2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0E2.
ExpressionAtlasiQ9R0E2. baseline and differential.
GenevisibleiQ9R0E2. MM.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cDNAs for mouse lysyl hydroxylase 1, 2 and 3, their phylogenetic analysis and tissue-specific expression in the mouse."
    Ruotsalainen H., Sipila L., Kerkela E., Pospiech H., Myllylae R.
    Matrix Biol. 18:325-329(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-198.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver and Lung.

Entry informationi

Entry nameiPLOD1_MOUSE
AccessioniPrimary (citable) accession number: Q9R0E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.