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Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3

Gene

Plod3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.By similarity

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Fe2+By similarity
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi670IronPROSITE-ProRule annotation1
Metal bindingi672IronPROSITE-ProRule annotation1
Metal bindingi722IronPROSITE-ProRule annotation1
Active sitei732Sequence analysis1

GO - Molecular functioni

  • iron ion binding Source: InterPro
  • L-ascorbic acid binding Source: UniProtKB-KW
  • procollagen glucosyltransferase activity Source: MGI
  • procollagen-lysine 5-dioxygenase activity Source: MGI

GO - Biological processi

  • basement membrane assembly Source: MGI
  • cellular response to hormone stimulus Source: Ensembl
  • collagen fibril organization Source: MGI
  • collagen metabolic process Source: MGI
  • endothelial cell morphogenesis Source: MGI
  • epidermis morphogenesis Source: MGI
  • hydroxylysine biosynthetic process Source: MGI
  • in utero embryonic development Source: MGI
  • lung morphogenesis Source: MGI
  • neural tube development Source: MGI
  • peptidyl-lysine hydroxylation Source: MGI
  • protein localization Source: MGI
  • protein O-linked glycosylation Source: MGI
  • vasodilation Source: MGI

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.4 3474
2.4.1.66 3474
ReactomeiR-MMU-1650814 Collagen biosynthesis and modifying enzymes

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (EC:1.14.11.4By similarity)
Alternative name(s):
Lysyl hydroxylase 3
Short name:
LH3
Gene namesi
Name:Plod3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1347008 Plod3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000002468728 – 741Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi286N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi551N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9R0E1
MaxQBiQ9R0E1
PaxDbiQ9R0E1
PeptideAtlasiQ9R0E1
PRIDEiQ9R0E1

PTM databases

iPTMnetiQ9R0E1
PhosphoSitePlusiQ9R0E1

Expressioni

Tissue specificityi

Highly expressed in the heart, lung, liver and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000004846
ExpressionAtlasiQ9R0E1 baseline and differential
GenevisibleiQ9R0E1 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi2049851 interactor.
IntActiQ9R0E1 2 interactors.
MINTiQ9R0E1
STRINGi10090.ENSMUSP00000004968

Structurei

3D structure databases

ProteinModelPortaliQ9R0E1
SMRiQ9R0E1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini650 – 741Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST92

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971 Eukaryota
ENOG410Y4QU LUCA
GeneTreeiENSGT00550000074427
HOGENOMiHOG000231099
HOVERGENiHBG053618
InParanoidiQ9R0E1
KOiK13646
OMAiLFYTRLY
OrthoDBiEOG091G02DK
PhylomeDBiQ9R0E1
TreeFamiTF313826

Family and domain databases

InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR006620 Pro_4_hyd_alph
IPR001006 Procol_lys_dOase
PfamiView protein in Pfam
PF03171 2OG-FeII_Oxy, 1 hit
SMARTiView protein in SMART
SM00702 P4Hc, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit
PS01325 LYS_HYDROXYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAGPEPRL LLLLLLLLPP LPPVTSASDR PRGANAVNPD KLLVITVATA
60 70 80 90 100
ETEGYRRFLQ SAEFFNYTVR TLGLGQEWRG GDVARTVGGG QKVRWLKKEM
110 120 130 140 150
EKYADQKDMI IMFVDSYDVI LASSPTELLK KFVQSGSHLL FSAESFCWPE
160 170 180 190 200
WGLAEQYPEV GMGKRFLNSG GFIGFAPTIH QIVRQWNYKD DDDDQLFYTQ
210 220 230 240 250
LYLDPGLREK LKLSLDHKSR IFQNLNGALD EVILKFDQNR VRIRNVAYDT
260 270 280 290 300
LPVVVHGNGP TKLQLNYLGN YVPNGWTPQG GCGFCNQTLR TLPGGQPPPR
310 320 330 340 350
VLLAVFVEQP TPFLPRFLQR LLLLDYPPDR ISLFLHNSEV YHEPHIADAW
360 370 380 390 400
PQLQDHFSAV KLVGPEEALS AGEARDMAMD SCRQNPECEF YFSLDADAVL
410 420 430 440 450
TNPETLRVLI EQNRKVIAPM LSRHGKLWSN FWGALSPNEY YARSEDYVEL
460 470 480 490 500
VQRKRVGVWN VPYISQAYVI RGETLRTELP QKEVFSSSDT DPDMAFCKSV
510 520 530 540 550
RDKGIFLHLS NQHEFGRLLA TSRYDTDHLH PDLWQIFDNP VDWREQYIHE
560 570 580 590 600
NYSRALDGEG LVEQPCPDVY WFPLLTEQMC DELVEEMEHY GQWSGGRHED
610 620 630 640 650
SRLAGGYENV PTVDIHMKQV GYEDQWLQLL RTYVGPMTEY LFPGYHTKTR
660 670 680 690 700
AVMNFVVRYR PDEQPSLRPH HDSSTFTLNV ALNHKGVDYE GGGCRFLRYD
710 720 730 740
CRISSPRKGW ALLHPGRLTH YHEGLPTTRG TRYIMVSFVD P
Length:741
Mass (Da):84,922
Last modified:May 1, 2000 - v1
Checksum:iD1B79B386339D9F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8P → H in BAB28704 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046783 mRNA Translation: AAD54618.1
AY014830 Genomic DNA Translation: AAK00576.1
AK013195 mRNA Translation: BAB28704.1
AK033360 mRNA Translation: BAC28246.1
AK088948 mRNA Translation: BAC40669.1
BC043047 mRNA Translation: AAH43047.1
BC054734 mRNA Translation: AAH54734.1
CCDSiCCDS19759.1
RefSeqiNP_036092.1, NM_011962.3
UniGeneiMm.251003

Genome annotation databases

EnsembliENSMUST00000004968; ENSMUSP00000004968; ENSMUSG00000004846
GeneIDi26433
KEGGimmu:26433
UCSCiuc009abj.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPLOD3_MOUSE
AccessioniPrimary (citable) accession number: Q9R0E1
Secondary accession number(s): Q542E0, Q9CYY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 1, 2000
Last modified: February 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome