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Protein

Sigma non-opioid intracellular receptor 1

Gene

Sigmar1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration. Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria (By similarity).By similarity6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei126Important for ligand bindingBy similarity1
Sitei172Important for ligand bindingBy similarity1

GO - Molecular functioni

  • opioid receptor activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Lipid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Sigma non-opioid intracellular receptor 1
Alternative name(s):
Sigma 1-type opioid receptor
Short name:
Sigma1-receptor
Short name:
Sigma1R
Gene namesi
Name:Sigmar1
Synonyms:Oprs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi68364. Sigmar1.

Subcellular locationi

  • Nucleus inner membrane By similarity
  • Nucleus outer membrane By similarity
  • Nucleus envelope By similarity
  • Cytoplasmic vesicle By similarity
  • Endoplasmic reticulum membrane 1 Publication
  • Membrane By similarity; Single-pass membrane protein By similarity
  • Lipid droplet 1 Publication
  • Cell junction By similarity
  • Cell membrane By similarity
  • Cell projectiongrowth cone By similarity
  • Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity

  • Note: During interphase, detected at the inner and outer nuclear membrane and the endoplasmic reticulum. Detected on cytoplasmic vesicles during mitosis (By similarity). Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum (PubMed:15466698). Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding. In motor neurons it is enriched at cholinergic postsynaptic densities (By similarity).By similarity1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9LumenalBy similarity9
Transmembranei10 – 30HelicalBy similarityAdd BLAST21
Topological domaini31 – 223CytoplasmicBy similarityAdd BLAST193

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Lipid droplet, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3602.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686551 – 223Sigma non-opioid intracellular receptor 1Add BLAST223

Proteomic databases

PaxDbiQ9R0C9.
PeptideAtlasiQ9R0C9.
PRIDEiQ9R0C9.

Expressioni

Tissue specificityi

Expressed in ependymocytes and neurons throughout the CNS from the olfactory bulb to the spinal cord. Expressed by progenitor, mature and satellite oligodendrocytes and by Schwann cells (at protein level). Expressed in liver, intestine, kidney, brain, lung and heart. Expressed by retinal cells.5 Publications

Gene expression databases

BgeeiENSRNOG00000014604.
GenevisibleiQ9R0C9. RN.

Interactioni

Subunit structurei

Homotrimer (By similarity). Interacts with KCNA2; cocaine consumption leads to increased interaction (By similarity). Forms a ternary complex with ANK2 and ITPR3. The complex is disrupted by agonists (PubMed:11149946). Interacts with KCNA4 (PubMed:11988171).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Hspa5P067613EBI-1557826,EBI-916036
Kcna2P631413EBI-1557826,EBI-644033From a different organism.

Protein-protein interaction databases

IntActiQ9R0C9. 3 interactors.
STRINGi10116.ENSRNOP00000019795.

Chemistry databases

BindingDBiQ9R0C9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 8Targeting to endoplasmic reticulum-associated lipid dropletsBy similarity7
Regioni99 – 106Important for ligand-bindingBy similarity8
Regioni177 – 223C-terminal hydrophobic regionCuratedAdd BLAST47

Domaini

The C-terminal helices form a flat, hydrophobic surface that is probably tightly associated with the cytosolic surface of the endoplasmic reticulum membrane.By similarity

Sequence similaritiesi

Belongs to the ERG2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4143. Eukaryota.
ENOG410XP0Z. LUCA.
GeneTreeiENSGT00390000012082.
HOVERGENiHBG058220.
InParanoidiQ9R0C9.
KOiK20719.
OMAiLFYTLRA.
OrthoDBiEOG091G0KD7.
PhylomeDBiQ9R0C9.
TreeFamiTF300106.

Family and domain databases

InterProiIPR006716. ERG2_sigma1_rcpt-like.
[Graphical view]
PfamiPF04622. ERG2_Sigma1R. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0C9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPWAVGRRWA WITLFLTIVA VLIQAVWLWL GTQSFVFQRE EIAQLARQYA
60 70 80 90 100
GLDHELAFSR LIVELRRLHP GHVLPDEELQ WVFVNAGGWM GAMCLLHASL
110 120 130 140 150
SEYVLLFGTA LGSHGHSGRY WAEISDTIIS GTFHQWREGT TKSEVYYPGE
160 170 180 190 200
TVVHGPGEAT AVEWGPNTWM VEYGRGVIPS TLAFALSDTI FSTQDFLTLF
210 220
YTLRAYARGL RLELTTYLFG QDP
Length:223
Mass (Da):25,270
Last modified:May 1, 2000 - v1
Checksum:iB125A0388F1FFC6E
GO
Isoform 2 (identifier: Q9R0C9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-106: YVLL → TILG
     107-223: Missing.

Show »
Length:106
Mass (Da):12,153
Checksum:i8BA4159B6A9E2A0F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161A → D in AAD01198 (PubMed:9489711).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021988103 – 106YVLL → TILG in isoform 2. 1 Publication4
Alternative sequenceiVSP_021989107 – 223Missing in isoform 2. 1 PublicationAdd BLAST117

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004218 mRNA. Translation: AAD01198.1.
AF067769 mRNA. Translation: AAF08342.1.
AF087827 mRNA. Translation: AAD49439.1.
BC061978 mRNA. Translation: AAH61978.1.
RefSeqiNP_112258.1. NM_030996.1.
UniGeneiRn.1129.

Genome annotation databases

EnsembliENSRNOT00000019795; ENSRNOP00000019795; ENSRNOG00000014604. [Q9R0C9-1]
GeneIDi29336.
KEGGirno:29336.
UCSCiRGD:68364. rat. [Q9R0C9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004218 mRNA. Translation: AAD01198.1.
AF067769 mRNA. Translation: AAF08342.1.
AF087827 mRNA. Translation: AAD49439.1.
BC061978 mRNA. Translation: AAH61978.1.
RefSeqiNP_112258.1. NM_030996.1.
UniGeneiRn.1129.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R0C9. 3 interactors.
STRINGi10116.ENSRNOP00000019795.

Chemistry databases

BindingDBiQ9R0C9.
ChEMBLiCHEMBL3602.

Proteomic databases

PaxDbiQ9R0C9.
PeptideAtlasiQ9R0C9.
PRIDEiQ9R0C9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019795; ENSRNOP00000019795; ENSRNOG00000014604. [Q9R0C9-1]
GeneIDi29336.
KEGGirno:29336.
UCSCiRGD:68364. rat. [Q9R0C9-1]

Organism-specific databases

CTDi10280.
RGDi68364. Sigmar1.

Phylogenomic databases

eggNOGiKOG4143. Eukaryota.
ENOG410XP0Z. LUCA.
GeneTreeiENSGT00390000012082.
HOVERGENiHBG058220.
InParanoidiQ9R0C9.
KOiK20719.
OMAiLFYTLRA.
OrthoDBiEOG091G0KD7.
PhylomeDBiQ9R0C9.
TreeFamiTF300106.

Miscellaneous databases

PROiQ9R0C9.

Gene expression databases

BgeeiENSRNOG00000014604.
GenevisibleiQ9R0C9. RN.

Family and domain databases

InterProiIPR006716. ERG2_sigma1_rcpt-like.
[Graphical view]
PfamiPF04622. ERG2_Sigma1R. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSGMR1_RAT
AccessioniPrimary (citable) accession number: Q9R0C9
Secondary accession number(s): Q9R1J7, Q9Z2W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Depletion by RNAi alters oligodendrocyte differentiation and enhances opioid analgesia.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.