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Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

Gene

Plod2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi666IronPROSITE-ProRule annotation1
Metal bindingi668IronPROSITE-ProRule annotation1
Metal bindingi718IronPROSITE-ProRule annotation1
Active sitei728Sequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (EC:1.14.11.4)
Alternative name(s):
Lysyl hydroxylase 2
Short name:
LH2
Gene namesi
Name:Plod2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1347007. Plod2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002468426 – 737Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2Add BLAST712

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Modified residuei320PhosphothreonineBy similarity1
Modified residuei323PhosphotyrosineBy similarity1
Glycosylationi365N-linked (GlcNAc...)Sequence analysis1
Glycosylationi522N-linked (GlcNAc...)Sequence analysis1
Glycosylationi696N-linked (GlcNAc...)Sequence analysis1
Modified residuei704N6-succinyllysineCombined sources1
Glycosylationi725N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9R0B9.
MaxQBiQ9R0B9.
PaxDbiQ9R0B9.
PeptideAtlasiQ9R0B9.
PRIDEiQ9R0B9.

PTM databases

iPTMnetiQ9R0B9.
PhosphoSitePlusiQ9R0B9.

Expressioni

Tissue specificityi

Is highly expressed in the heart, lung, kidney, eye, ovary and placenta.1 Publication

Gene expression databases

BgeeiENSMUSG00000032374.
ExpressionAtlasiQ9R0B9. baseline and differential.
GenevisibleiQ9R0B9. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi204984. 1 interactor.
IntActiQ9R0B9. 1 interactor.
MINTiMINT-4107957.
STRINGi10090.ENSMUSP00000125373.

Structurei

3D structure databases

ProteinModelPortaliQ9R0B9.
SMRiQ9R0B9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini644 – 737Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST94

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971. Eukaryota.
ENOG410Y4QU. LUCA.
GeneTreeiENSGT00550000074427.
HOGENOMiHOG000231099.
HOVERGENiHBG053618.
InParanoidiQ9R0B9.
KOiK13645.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0B9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDRGARPGR LMPMLALLSW AAGLGVAEET PGRIPADKLL VITVATKEND
60 70 80 90 100
GFHRFMNSAK YFNYTVKVLG QGQEWRGGDG MNSIGGGQKV RLLKEAMEHY
110 120 130 140 150
ASQEDLVILF TECFDVVFAG GPEEVLKKFQ KTNHKIVFAA DGLLWPDKRL
160 170 180 190 200
ADKYPVVHIG KRYLNSGGFI GYAPYISRLV QQWNLQDNDD DQLFYTKVYI
210 220 230 240 250
DPLKREAFNI TLDHKCKIFQ ALNGATDEVV LKFENGKSRV KNTFYETLPV
260 270 280 290 300
AINGNGPTKI LLNYFGNYVP NSWTQENGCA LCDVDTIDLS TVDVPPKVTL
310 320 330 340 350
GVFIEQPTPF LPRFLNLLLT LDYPKEALQL FIHNKEVYHE KDIKVFVDKA
360 370 380 390 400
KHDISSIKIV GPEENLSQAE ARNMGMDFCR QDEKCDYYFS VDADVVLTNP
410 420 430 440 450
RTLKFLIEQN RKIIAPLVTR HGKLWSNFWG ALSPDGYYAR SEDYVDIVQG
460 470 480 490 500
NRVGIWNVPY MANVYLIQGK TLRSEMNERN YFVRDKLDPD MALCRNARDM
510 520 530 540 550
GVFMYISNRH EFGRLISTAN YNTSHLNNDF WQIFENPVDW KEKYINRDYS
560 570 580 590 600
KIFTENIVEQ PCPDVFWFPI FSERACDELV EEMEHYGKWS GGKHHDSRIS
610 620 630 640 650
GGYENVPTDD IHMKQIGLEN VWLHFIREFI APVTLKVFAG YYTKGFALLN
660 670 680 690 700
FVVKYSPERQ RSLRPHHDAS TFTINIALNN VGEDFQGGGC KFLRYNCSIE
710 720 730
SPRKGWSFMH PGRLTHLHEG LPVKNGTRYI AVSFIDP
Length:737
Mass (Da):84,488
Last modified:July 27, 2011 - v2
Checksum:i0FF2880E5FEEA084
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2G → E in AAD53987 (PubMed:10429951).Curated1
Sequence conflicti405F → I in AAH21352 (PubMed:15489334).Curated1
Sequence conflicti611I → T in AAD53987 (PubMed:10429951).Curated1
Sequence conflicti611I → T in AAH21352 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080572 mRNA. Translation: AAD53987.1.
AC165156 Genomic DNA. No translation available.
BC021352 mRNA. Translation: AAH21352.1.
CCDSiCCDS23408.1.
RefSeqiNP_001136388.1. NM_001142916.1.
NP_036091.2. NM_011961.3.
UniGeneiMm.79983.

Genome annotation databases

EnsembliENSMUST00000070522; ENSMUSP00000068611; ENSMUSG00000032374.
GeneIDi26432.
KEGGimmu:26432.
UCSCiuc009rau.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080572 mRNA. Translation: AAD53987.1.
AC165156 Genomic DNA. No translation available.
BC021352 mRNA. Translation: AAH21352.1.
CCDSiCCDS23408.1.
RefSeqiNP_001136388.1. NM_001142916.1.
NP_036091.2. NM_011961.3.
UniGeneiMm.79983.

3D structure databases

ProteinModelPortaliQ9R0B9.
SMRiQ9R0B9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204984. 1 interactor.
IntActiQ9R0B9. 1 interactor.
MINTiMINT-4107957.
STRINGi10090.ENSMUSP00000125373.

PTM databases

iPTMnetiQ9R0B9.
PhosphoSitePlusiQ9R0B9.

Proteomic databases

EPDiQ9R0B9.
MaxQBiQ9R0B9.
PaxDbiQ9R0B9.
PeptideAtlasiQ9R0B9.
PRIDEiQ9R0B9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070522; ENSMUSP00000068611; ENSMUSG00000032374.
GeneIDi26432.
KEGGimmu:26432.
UCSCiuc009rau.2. mouse.

Organism-specific databases

CTDi5352.
MGIiMGI:1347007. Plod2.

Phylogenomic databases

eggNOGiKOG1971. Eukaryota.
ENOG410Y4QU. LUCA.
GeneTreeiENSGT00550000074427.
HOGENOMiHOG000231099.
HOVERGENiHBG053618.
InParanoidiQ9R0B9.
KOiK13645.

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

ChiTaRSiPlod2. mouse.
PROiQ9R0B9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032374.
ExpressionAtlasiQ9R0B9. baseline and differential.
GenevisibleiQ9R0B9. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLOD2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0B9
Secondary accession number(s): E9QM54, Q8VDT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.