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Q9R0B9 (PLOD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
EC=1.14.11.4
Alternative name(s):
Lysyl hydroxylase 2
Short name=LH2
Gene names
Name:Plod2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length737 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

Catalytic activity

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.

Cofactor

Iron By similarity.

Ascorbate By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.

Tissue specificity

Is highly expressed in the heart, lung, kidney, eye, ovary and placenta. Ref.1

Sequence similarities

Contains 1 Fe2OG dioxygenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 737712Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
PRO_0000024684

Regions

Domain644 – 73794Fe2OG dioxygenase

Sites

Active site7281 Potential
Metal binding6661Iron By similarity
Metal binding6681Iron By similarity
Metal binding7181Iron By similarity

Amino acid modifications

Modified residue3201Phosphothreonine By similarity
Modified residue3231Phosphotyrosine By similarity
Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation2091N-linked (GlcNAc...) Potential
Glycosylation3651N-linked (GlcNAc...) Potential
Glycosylation5221N-linked (GlcNAc...) Potential
Glycosylation6961N-linked (GlcNAc...) Potential
Glycosylation7251N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict21G → E in AAD53987. Ref.1
Sequence conflict4051F → I in AAH21352. Ref.3
Sequence conflict6111I → T in AAD53987. Ref.1
Sequence conflict6111I → T in AAH21352. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9R0B9 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 0FF2880E5FEEA084

FASTA73784,488
        10         20         30         40         50         60 
MGDRGARPGR LMPMLALLSW AAGLGVAEET PGRIPADKLL VITVATKEND GFHRFMNSAK 

        70         80         90        100        110        120 
YFNYTVKVLG QGQEWRGGDG MNSIGGGQKV RLLKEAMEHY ASQEDLVILF TECFDVVFAG 

       130        140        150        160        170        180 
GPEEVLKKFQ KTNHKIVFAA DGLLWPDKRL ADKYPVVHIG KRYLNSGGFI GYAPYISRLV 

       190        200        210        220        230        240 
QQWNLQDNDD DQLFYTKVYI DPLKREAFNI TLDHKCKIFQ ALNGATDEVV LKFENGKSRV 

       250        260        270        280        290        300 
KNTFYETLPV AINGNGPTKI LLNYFGNYVP NSWTQENGCA LCDVDTIDLS TVDVPPKVTL 

       310        320        330        340        350        360 
GVFIEQPTPF LPRFLNLLLT LDYPKEALQL FIHNKEVYHE KDIKVFVDKA KHDISSIKIV 

       370        380        390        400        410        420 
GPEENLSQAE ARNMGMDFCR QDEKCDYYFS VDADVVLTNP RTLKFLIEQN RKIIAPLVTR 

       430        440        450        460        470        480 
HGKLWSNFWG ALSPDGYYAR SEDYVDIVQG NRVGIWNVPY MANVYLIQGK TLRSEMNERN 

       490        500        510        520        530        540 
YFVRDKLDPD MALCRNARDM GVFMYISNRH EFGRLISTAN YNTSHLNNDF WQIFENPVDW 

       550        560        570        580        590        600 
KEKYINRDYS KIFTENIVEQ PCPDVFWFPI FSERACDELV EEMEHYGKWS GGKHHDSRIS 

       610        620        630        640        650        660 
GGYENVPTDD IHMKQIGLEN VWLHFIREFI APVTLKVFAG YYTKGFALLN FVVKYSPERQ 

       670        680        690        700        710        720 
RSLRPHHDAS TFTINIALNN VGEDFQGGGC KFLRYNCSIE SPRKGWSFMH PGRLTHLHEG 

       730 
LPVKNGTRYI AVSFIDP

« Hide

References

« Hide 'large scale' references
[1]"Characterization of cDNAs for mouse lysyl hydroxylase 1, 2 and 3, their phylogenetic analysis and tissue-specific expression in the mouse."
Ruotsalainen H., Sipila L., Kerkela E., Pospiech H., Myllylae R.
Matrix Biol. 18:325-329(1999) [PubMed: 10429951] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF080572 mRNA. Translation: AAD53987.1.
AC165156 Genomic DNA. No translation available.
BC021352 mRNA. Translation: AAH21352.1.
IPIIPI00918500.
RefSeqNP_001136388.1. NM_001142916.1.
NP_036091.2. NM_011961.3.
UniGeneMm.79983.

3D structure databases

ProteinModelPortalQ9R0B9.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9R0B9.

PTM databases

PhosphoSiteQ9R0B9.

Proteomic databases

PRIDEQ9R0B9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070522; ENSMUSP00000068611; ENSMUSG00000032374.
GeneID26432.
KEGGmmu:26432.
UCSCuc009rau.2. mouse.

Organism-specific databases

CTD5352.
MGIMGI:1347007. Plod2.

Phylogenomic databases

eggNOGroNOG07750.
GeneTreeENSGT00550000074427.
HOVERGENHBG053618.
InParanoidQ9R0B9.
OrthoDBEOG4KKZ2D.

Gene expression databases

ArrayExpressQ9R0B9.
BgeeQ9R0B9.
GenevestigatorQ9R0B9.
GermOnlineENSMUSG00000032374. Mus musculus.

Family and domain databases

InterProIPR005123. Oxoglutarate/Fe-dep_oxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
[Graphical view]
KOK13645.
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio304489.
SOURCESearch...

Entry information

Entry namePLOD2_MOUSE
AccessionPrimary (citable) accession number: Q9R0B9
Secondary accession number(s): E9QM54, Q8VDT4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: July 27, 2011
Last modified: November 16, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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