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Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

Gene

Plod2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.By similarity

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Fe2+By similarity
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi666IronPROSITE-ProRule annotation1
Metal bindingi668IronPROSITE-ProRule annotation1
Metal bindingi718IronPROSITE-ProRule annotation1
Active sitei728Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiR-MMU-1650814 Collagen biosynthesis and modifying enzymes

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (EC:1.14.11.4By similarity)
Alternative name(s):
Lysyl hydroxylase 2
Short name:
LH2
Gene namesi
Name:Plod2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1347007 Plod2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002468426 – 737Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2Add BLAST712

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei320PhosphothreonineBy similarity1
Modified residuei323PhosphotyrosineBy similarity1
Glycosylationi365N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi522N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi696N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei704N6-succinyllysineCombined sources1
Glycosylationi725N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9R0B9
MaxQBiQ9R0B9
PaxDbiQ9R0B9
PeptideAtlasiQ9R0B9
PRIDEiQ9R0B9

PTM databases

iPTMnetiQ9R0B9
PhosphoSitePlusiQ9R0B9

Expressioni

Tissue specificityi

Is highly expressed in the heart, lung, kidney, eye, ovary and placenta.1 Publication

Gene expression databases

BgeeiENSMUSG00000032374
ExpressionAtlasiQ9R0B9 baseline and differential
GenevisibleiQ9R0B9 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi204984, 1 interactor
IntActiQ9R0B9, 1 interactor
MINTiQ9R0B9
STRINGi10090.ENSMUSP00000125373

Structurei

3D structure databases

ProteinModelPortaliQ9R0B9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini644 – 737Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST94

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971 Eukaryota
ENOG410Y4QU LUCA
GeneTreeiENSGT00550000074427
HOGENOMiHOG000231099
HOVERGENiHBG053618
InParanoidiQ9R0B9
KOiK13645

Family and domain databases

InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR006620 Pro_4_hyd_alph
IPR001006 Procol_lys_dOase
PfamiView protein in Pfam
PF03171 2OG-FeII_Oxy, 1 hit
SMARTiView protein in SMART
SM00702 P4Hc, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit
PS01325 LYS_HYDROXYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0B9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDRGARPGR LMPMLALLSW AAGLGVAEET PGRIPADKLL VITVATKEND
60 70 80 90 100
GFHRFMNSAK YFNYTVKVLG QGQEWRGGDG MNSIGGGQKV RLLKEAMEHY
110 120 130 140 150
ASQEDLVILF TECFDVVFAG GPEEVLKKFQ KTNHKIVFAA DGLLWPDKRL
160 170 180 190 200
ADKYPVVHIG KRYLNSGGFI GYAPYISRLV QQWNLQDNDD DQLFYTKVYI
210 220 230 240 250
DPLKREAFNI TLDHKCKIFQ ALNGATDEVV LKFENGKSRV KNTFYETLPV
260 270 280 290 300
AINGNGPTKI LLNYFGNYVP NSWTQENGCA LCDVDTIDLS TVDVPPKVTL
310 320 330 340 350
GVFIEQPTPF LPRFLNLLLT LDYPKEALQL FIHNKEVYHE KDIKVFVDKA
360 370 380 390 400
KHDISSIKIV GPEENLSQAE ARNMGMDFCR QDEKCDYYFS VDADVVLTNP
410 420 430 440 450
RTLKFLIEQN RKIIAPLVTR HGKLWSNFWG ALSPDGYYAR SEDYVDIVQG
460 470 480 490 500
NRVGIWNVPY MANVYLIQGK TLRSEMNERN YFVRDKLDPD MALCRNARDM
510 520 530 540 550
GVFMYISNRH EFGRLISTAN YNTSHLNNDF WQIFENPVDW KEKYINRDYS
560 570 580 590 600
KIFTENIVEQ PCPDVFWFPI FSERACDELV EEMEHYGKWS GGKHHDSRIS
610 620 630 640 650
GGYENVPTDD IHMKQIGLEN VWLHFIREFI APVTLKVFAG YYTKGFALLN
660 670 680 690 700
FVVKYSPERQ RSLRPHHDAS TFTINIALNN VGEDFQGGGC KFLRYNCSIE
710 720 730
SPRKGWSFMH PGRLTHLHEG LPVKNGTRYI AVSFIDP
Length:737
Mass (Da):84,488
Last modified:July 27, 2011 - v2
Checksum:i0FF2880E5FEEA084
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2G → E in AAD53987 (PubMed:10429951).Curated1
Sequence conflicti405F → I in AAH21352 (PubMed:15489334).Curated1
Sequence conflicti611I → T in AAD53987 (PubMed:10429951).Curated1
Sequence conflicti611I → T in AAH21352 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080572 mRNA Translation: AAD53987.1
AC165156 Genomic DNA No translation available.
BC021352 mRNA Translation: AAH21352.1
CCDSiCCDS23408.1
RefSeqiNP_001136388.1, NM_001142916.1
NP_036091.2, NM_011961.3
UniGeneiMm.79983

Genome annotation databases

EnsembliENSMUST00000070522; ENSMUSP00000068611; ENSMUSG00000032374
GeneIDi26432
KEGGimmu:26432
UCSCiuc009rau.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPLOD2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0B9
Secondary accession number(s): E9QM54, Q8VDT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health