Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

Gene

Plod2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi666 – 6661IronPROSITE-ProRule annotation
Metal bindingi668 – 6681IronPROSITE-ProRule annotation
Metal bindingi718 – 7181IronPROSITE-ProRule annotation
Active sitei728 – 7281Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (EC:1.14.11.4)
Alternative name(s):
Lysyl hydroxylase 2
Short name:
LH2
Gene namesi
Name:Plod2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1347007. Plod2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 737712Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2PRO_0000024684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence analysis
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence analysis
Modified residuei320 – 3201PhosphothreonineBy similarity
Modified residuei323 – 3231PhosphotyrosineBy similarity
Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence analysis
Glycosylationi522 – 5221N-linked (GlcNAc...)Sequence analysis
Glycosylationi696 – 6961N-linked (GlcNAc...)Sequence analysis
Modified residuei704 – 7041N6-succinyllysineCombined sources
Glycosylationi725 – 7251N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9R0B9.
MaxQBiQ9R0B9.
PaxDbiQ9R0B9.
PeptideAtlasiQ9R0B9.
PRIDEiQ9R0B9.

PTM databases

iPTMnetiQ9R0B9.
PhosphoSiteiQ9R0B9.

Expressioni

Tissue specificityi

Is highly expressed in the heart, lung, kidney, eye, ovary and placenta.1 Publication

Gene expression databases

BgeeiENSMUSG00000032374.
ExpressionAtlasiQ9R0B9. baseline and differential.
GenevisibleiQ9R0B9. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi204984. 1 interaction.
IntActiQ9R0B9. 1 interaction.
MINTiMINT-4107957.
STRINGi10090.ENSMUSP00000125373.

Structurei

3D structure databases

ProteinModelPortaliQ9R0B9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini644 – 73794Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971. Eukaryota.
ENOG410Y4QU. LUCA.
GeneTreeiENSGT00550000074427.
HOGENOMiHOG000231099.
HOVERGENiHBG053618.
InParanoidiQ9R0B9.
KOiK13645.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0B9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDRGARPGR LMPMLALLSW AAGLGVAEET PGRIPADKLL VITVATKEND
60 70 80 90 100
GFHRFMNSAK YFNYTVKVLG QGQEWRGGDG MNSIGGGQKV RLLKEAMEHY
110 120 130 140 150
ASQEDLVILF TECFDVVFAG GPEEVLKKFQ KTNHKIVFAA DGLLWPDKRL
160 170 180 190 200
ADKYPVVHIG KRYLNSGGFI GYAPYISRLV QQWNLQDNDD DQLFYTKVYI
210 220 230 240 250
DPLKREAFNI TLDHKCKIFQ ALNGATDEVV LKFENGKSRV KNTFYETLPV
260 270 280 290 300
AINGNGPTKI LLNYFGNYVP NSWTQENGCA LCDVDTIDLS TVDVPPKVTL
310 320 330 340 350
GVFIEQPTPF LPRFLNLLLT LDYPKEALQL FIHNKEVYHE KDIKVFVDKA
360 370 380 390 400
KHDISSIKIV GPEENLSQAE ARNMGMDFCR QDEKCDYYFS VDADVVLTNP
410 420 430 440 450
RTLKFLIEQN RKIIAPLVTR HGKLWSNFWG ALSPDGYYAR SEDYVDIVQG
460 470 480 490 500
NRVGIWNVPY MANVYLIQGK TLRSEMNERN YFVRDKLDPD MALCRNARDM
510 520 530 540 550
GVFMYISNRH EFGRLISTAN YNTSHLNNDF WQIFENPVDW KEKYINRDYS
560 570 580 590 600
KIFTENIVEQ PCPDVFWFPI FSERACDELV EEMEHYGKWS GGKHHDSRIS
610 620 630 640 650
GGYENVPTDD IHMKQIGLEN VWLHFIREFI APVTLKVFAG YYTKGFALLN
660 670 680 690 700
FVVKYSPERQ RSLRPHHDAS TFTINIALNN VGEDFQGGGC KFLRYNCSIE
710 720 730
SPRKGWSFMH PGRLTHLHEG LPVKNGTRYI AVSFIDP
Length:737
Mass (Da):84,488
Last modified:July 27, 2011 - v2
Checksum:i0FF2880E5FEEA084
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21G → E in AAD53987 (PubMed:10429951).Curated
Sequence conflicti405 – 4051F → I in AAH21352 (PubMed:15489334).Curated
Sequence conflicti611 – 6111I → T in AAD53987 (PubMed:10429951).Curated
Sequence conflicti611 – 6111I → T in AAH21352 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080572 mRNA. Translation: AAD53987.1.
AC165156 Genomic DNA. No translation available.
BC021352 mRNA. Translation: AAH21352.1.
CCDSiCCDS23408.1.
RefSeqiNP_001136388.1. NM_001142916.1.
NP_036091.2. NM_011961.3.
UniGeneiMm.79983.

Genome annotation databases

EnsembliENSMUST00000070522; ENSMUSP00000068611; ENSMUSG00000032374.
GeneIDi26432.
KEGGimmu:26432.
UCSCiuc009rau.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080572 mRNA. Translation: AAD53987.1.
AC165156 Genomic DNA. No translation available.
BC021352 mRNA. Translation: AAH21352.1.
CCDSiCCDS23408.1.
RefSeqiNP_001136388.1. NM_001142916.1.
NP_036091.2. NM_011961.3.
UniGeneiMm.79983.

3D structure databases

ProteinModelPortaliQ9R0B9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204984. 1 interaction.
IntActiQ9R0B9. 1 interaction.
MINTiMINT-4107957.
STRINGi10090.ENSMUSP00000125373.

PTM databases

iPTMnetiQ9R0B9.
PhosphoSiteiQ9R0B9.

Proteomic databases

EPDiQ9R0B9.
MaxQBiQ9R0B9.
PaxDbiQ9R0B9.
PeptideAtlasiQ9R0B9.
PRIDEiQ9R0B9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070522; ENSMUSP00000068611; ENSMUSG00000032374.
GeneIDi26432.
KEGGimmu:26432.
UCSCiuc009rau.2. mouse.

Organism-specific databases

CTDi5352.
MGIiMGI:1347007. Plod2.

Phylogenomic databases

eggNOGiKOG1971. Eukaryota.
ENOG410Y4QU. LUCA.
GeneTreeiENSGT00550000074427.
HOGENOMiHOG000231099.
HOVERGENiHBG053618.
InParanoidiQ9R0B9.
KOiK13645.

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

ChiTaRSiPlod2. mouse.
PROiQ9R0B9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032374.
ExpressionAtlasiQ9R0B9. baseline and differential.
GenevisibleiQ9R0B9. MM.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLOD2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0B9
Secondary accession number(s): E9QM54, Q8VDT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.