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Q9R0B7 (ZN346_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 346
Alternative name(s):
Just another zinc finger protein
Gene names
Name:Znf346
Synonyms:Jaz, Zfp346
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds with low affinity to dsDNA and ssRNA, and with high affinity to dsRNA, with no detectable sequence specificity By similarity. Ref.1

Subunit structure

Forms a heteromeric complex with XPO5 and ILF3. Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and double-stranded RNA. Interacts with XPO5. Interacts with ILF3 in an RNA-independent manner By similarity.

Subcellular location

Nucleusnucleolus By similarity. Cytoplasm By similarity. Note: Nuclear at steady state, primarily in the nucleolus. Shuttles between the nucleus and cytoplasm when associated with XPO5 By similarity. Ref.1

Tissue specificity

Expressed in all tissues tested, including heart, brain, spleen, lung, liver, muscle, kidney and testis. Exogenous expression induced apoptosis. Ref.1

Domain

The zinc-finger domains are required for binding to dsRNA, and also for nuclear localization.

Sequence similarities

Contains 4 matrin-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Zinc finger protein 346
PRO_0000191810

Regions

Zinc finger70 – 10435Matrin-type 1
Zinc finger131 – 16535Matrin-type 2
Zinc finger182 – 21635Matrin-type 3
Zinc finger236 – 27035Matrin-type 4

Sites

Metal binding751Zinc 1 By similarity
Metal binding781Zinc 1 By similarity
Metal binding911Zinc 1; via tele nitrogen By similarity
Metal binding971Zinc 1; via pros nitrogen By similarity
Metal binding1361Zinc 2 By similarity
Metal binding1391Zinc 2 By similarity
Metal binding1521Zinc 2; via tele nitrogen By similarity
Metal binding1581Zinc 2; via pros nitrogen By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Experimental info

Mutagenesis911H → A: No loss of dsRNA binding capacity. Binding capacity was reduced 40-60%; when associated with A-203. Binding capacity was reduced to 5-10%; when associated with A-152 and A-203. Loss of binding capacity; when associated with A-152; A-203 and A-257. Ref.1
Mutagenesis1521H → A: No loss of dsRNA binding capacity. Loss of 40-60% dsRNA binding; when associated with A-203. Binding capacity was reduced to 5-10%; when associated with A-91 and A-203. Loss of binding capacity; when associated with A-91; A-203 and A-257. Ref.1
Mutagenesis2031H → A: No loss of dsRNA binding capacity. Binding capacity was reduced 40-60%; when associated with A-91. Binding capacity was reduced to 5-10%; when associated with A-91 and A-152. Loss of binding capacity; when associated with A-91; A-152 and A-257. Ref.1
Mutagenesis2571H → A: No loss of dsRNA binding capacity. Loss of 5-10 % dsRNA binding capacity; when associated with A-152 and A-203. Loss of binding capacity; when associated with A-91; A-152 and A-203. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9R0B7 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 6BD916262EDBA71E

FASTA29432,698
        10         20         30         40         50         60 
MECPAPDATD AADPGEAGPY KGSEEPEGRE PDGVRFDRER ARRLWEAVSG AQPAGREEVE 

        70         80         90        100        110        120 
HMIQKNQCLF TSTQCKVCCA MLISESQKLA HYQSKKHANK VKRYLAIHGM ETIKGDVKRL 

       130        140        150        160        170        180 
DSDQKSSRSK DKNHCCPICN MTFSSPAVAQ SHYLGKTHAK SLKLKQQSTK GAALQQNREM 

       190        200        210        220        230        240 
LDPDKFCSLC HSTFNDPAMA QQHYMGKRHR KQETKLKLMA HYGRLADPAV SDLPAGKGYP 

       250        260        270        280        290 
CKTCKIVLNS IEQYQAHVSG FKHKNQSPKT LVTLGSQTPV QTQPTPKDSS TVQD 

« Hide

References

« Hide 'large scale' references
[1]"JAZ requires the double-stranded RNA-binding zinc finger motifs for nuclear localization."
Yang M., May W.S., Ito T.
J. Biol. Chem. 274:27399-27406(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF HIS-91; HIS-152; HIS-203 AND HIS-257.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C3H/He.
Tissue: Osteoblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF083339 mRNA. Translation: AAD52017.1.
BC055075 mRNA. Translation: AAH55075.1.
CCDSCCDS26539.1.
RefSeqNP_036147.1. NM_012017.2.
UniGeneMm.22795.

3D structure databases

ProteinModelPortalQ9R0B7.
SMRQ9R0B7. Positions 53-167, 181-224, 239-267.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid205063. 1 interaction.
MINTMINT-4084241.

Proteomic databases

PaxDbQ9R0B7.
PRIDEQ9R0B7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021937; ENSMUSP00000021937; ENSMUSG00000021481.
GeneID26919.
KEGGmmu:26919.
UCSCuc007qpy.1. mouse.

Organism-specific databases

CTD26919.
MGIMGI:1349417. Zfp346.

Phylogenomic databases

eggNOGNOG85918.
GeneTreeENSGT00510000046606.
HOGENOMHOG000008682.
HOVERGENHBG061007.
InParanoidQ9R0B7.
PhylomeDBQ9R0B7.
TreeFamTF350019.

Gene expression databases

ArrayExpressQ9R0B7.
BgeeQ9R0B7.
CleanExMM_ZFP346.
GenevestigatorQ9R0B7.

Family and domain databases

InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 4 hits.
SM00451. ZnF_U1. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZNF346. mouse.
NextBio304801.
PROQ9R0B7.
SOURCESearch...

Entry information

Entry nameZN346_MOUSE
AccessionPrimary (citable) accession number: Q9R0B7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot