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Q9R0A5 (NEK3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase Nek3

EC=2.7.11.1
Alternative name(s):
Never in mitosis A-related kinase 3
Short name=NimA-related protein kinase 3
Gene names
Name:Nek3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Subunit structure

Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent By similarity.

Subcellular location

Cytoplasm. Cell projectionaxon Ref.6.

Tissue specificity

Brain. Ref.6

Developmental stage

Highly expressed during embryogenesis and early postnatal life, but is expressed at lower levels in adults. Ref.6

Post-translational modification

Phosphorylation at Thr-477 regulates its catalytic activity.

Sequence similarities

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 511511Serine/threonine-protein kinase Nek3
PRO_0000086424

Regions

Domain4 – 255252Protein kinase
Nucleotide binding10 – 189ATP By similarity
Region1 – 282282Interaction with VAV2 By similarity

Sites

Active site1251Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1591Phosphothreonine; by autocatalysis By similarity
Modified residue4771Phosphothreonine Ref.4 Ref.5 Ref.6

Experimental info

Mutagenesis4771T → A: Distorted neuronal morphology with disturbed polarity and deacetylation of microtubules. Ref.6
Sequence conflict951K → N in AAD20986. Ref.1
Sequence conflict951K → N in AAD16286. Ref.2
Sequence conflict2391N → K in AAD16286. Ref.2
Sequence conflict2961R → G in AAD20986. Ref.1
Sequence conflict2961R → G in AAD16286. Ref.2
Sequence conflict3061S → A in AAD20986. Ref.1
Sequence conflict3061S → A in AAD16286. Ref.2
Sequence conflict3341E → G in AAD20986. Ref.1
Sequence conflict3341E → G in AAD16286. Ref.2
Sequence conflict342 – 3432Missing in AAD16286. Ref.2
Sequence conflict3461P → R in AAD20986. Ref.1
Sequence conflict3461P → R in AAD16286. Ref.2
Sequence conflict3511R → S in AAD20986. Ref.1
Sequence conflict3511R → S in AAD16286. Ref.2
Sequence conflict3621W → R in AAD20986. Ref.1
Sequence conflict3621W → R in AAD16286. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9R0A5 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 31E777B3BFA31930

FASTA51157,464
        10         20         30         40         50         60 
MDNYTVLRVI GQGSFGRALL VLQESSNQTF AMKEIRLLKS DTQTSRKEAV LLAKMKHPNI 

        70         80         90        100        110        120 
VAFKESFEAE GYLYIVMEYC DGGDLMQRIK QQKGKLFPED TILNWFIQIC LGVNHIHKRR 

       130        140        150        160        170        180 
VLHRDIKSKN VFLTHNGKVK LGDFGSARLL SSPMAFACTY VGTPYYVPPE IWENLPYNNK 

       190        200        210        220        230        240 
SDIWSLGCIL YELCALKHPF QANSWKNLIL KICQGPIHPL PALYSCKLQG LVKQMLKRNP 

       250        260        270        280        290        300 
SHRPSATTLL CRGSLAPLVP KCLPPQIIRE YGEQILDEIK ISTPKNMKKQ DSNRVRRALG 

       310        320        330        340        350        360 
EANSASMQEE ERGRKCSHTE LESTGTTPAG NALERAARGN PESGNPQEHG RHTSPASPHR 

       370        380        390        400        410        420 
PWWERHGPSS NVEALEKASI LTSSFTAEDD RGGSVIKYEE NARRQWVREP PEALLSMLKD 

       430        440        450        460        470        480 
ADLSQAFQTY TIYRPGAEGF LKGPLSEDTA SDSVDGDLDS VMLDPERFEP RLDEEDTDFE 

       490        500        510 
EDNENPDWVS ELKKHVGYGD GPGGQLLGER A 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators."
Tanaka K., Nigg E.A.
J. Biol. Chem. 274:13491-13497(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3."
Chen A., Yanai A., Arama E., Kilfin G., Motro B.
Gene 234:127-137(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons."
Chang J., Baloh R.H., Milbrandt J.
J. Cell Sci. 122:2274-2282(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, PHOSPHORYLATION AT THR-477, MUTAGENESIS OF THR-477.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF093416 mRNA. Translation: AAD20986.1.
AF099066 mRNA. Translation: AAD16286.1.
AC117665 Genomic DNA. No translation available.
CCDSCCDS52519.1.
RefSeqNP_001156419.1. NM_001162947.1.
XP_006509174.1. XM_006509111.1.
UniGeneMm.41413.

3D structure databases

ProteinModelPortalQ9R0A5.
SMRQ9R0A5. Positions 1-290.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9R0A5.

Proteomic databases

PaxDbQ9R0A5.
PRIDEQ9R0A5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000110730; ENSMUSP00000106358; ENSMUSG00000031478.
ENSMUST00000178324; ENSMUSP00000136876; ENSMUSG00000031478.
GeneID23954.
KEGGmmu:23954.
UCSCuc012gbb.1. mouse.

Organism-specific databases

CTD4752.
MGIMGI:1344371. Nek3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00720000108476.
HOGENOMHOG000261617.
HOVERGENHBG003063.
InParanoidQ9R0A5.
KOK08857.
OMARQWEKNV.

Gene expression databases

ArrayExpressQ9R0A5.
BgeeQ9R0A5.
GenevestigatorQ9R0A5.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio303780.
PROQ9R0A5.
SOURCESearch...

Entry information

Entry nameNEK3_MOUSE
AccessionPrimary (citable) accession number: Q9R0A5
Secondary accession number(s): E9QLM2, Q9Z0X9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot