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Protein

Serine/threonine-protein kinase Nek3

Gene

Nek3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • establishment of cell polarity Source: CACAO
  • mitotic nuclear division Source: UniProtKB-KW
  • neuron projection morphogenesis Source: CACAO
  • protein phosphorylation Source: UniProtKB
  • regulation of tubulin deacetylation Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek3 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 3
Short name:
NimA-related protein kinase 3
Gene namesi
Name:Nek3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1344371. Nek3.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell projectionaxon 1 Publication

GO - Cellular componenti

  • axon Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi477 – 4771T → A: Distorted neuronal morphology with disturbed polarity and deacetylation of microtubules. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Serine/threonine-protein kinase Nek3PRO_0000086424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei159 – 1591Phosphothreonine; by autocatalysisBy similarity
Modified residuei477 – 4771Phosphothreonine3 Publications

Post-translational modificationi

Phosphorylation at Thr-477 regulates its catalytic activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9R0A5.
PaxDbiQ9R0A5.
PRIDEiQ9R0A5.

PTM databases

PhosphoSiteiQ9R0A5.

Expressioni

Tissue specificityi

Brain.1 Publication

Developmental stagei

Highly expressed during embryogenesis and early postnatal life, but is expressed at lower levels in adults.1 Publication

Gene expression databases

BgeeiQ9R0A5.
ExpressionAtlasiQ9R0A5. baseline and differential.
GenevisibleiQ9R0A5. MM.

Interactioni

Subunit structurei

Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000106358.

Structurei

3D structure databases

ProteinModelPortaliQ9R0A5.
SMRiQ9R0A5. Positions 1-290, 457-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 255252Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 282282Interaction with VAV2By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000261617.
HOVERGENiHBG003063.
InParanoidiQ9R0A5.
KOiK08857.
OMAiRQWEKNV.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0A5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNYTVLRVI GQGSFGRALL VLQESSNQTF AMKEIRLLKS DTQTSRKEAV
60 70 80 90 100
LLAKMKHPNI VAFKESFEAE GYLYIVMEYC DGGDLMQRIK QQKGKLFPED
110 120 130 140 150
TILNWFIQIC LGVNHIHKRR VLHRDIKSKN VFLTHNGKVK LGDFGSARLL
160 170 180 190 200
SSPMAFACTY VGTPYYVPPE IWENLPYNNK SDIWSLGCIL YELCALKHPF
210 220 230 240 250
QANSWKNLIL KICQGPIHPL PALYSCKLQG LVKQMLKRNP SHRPSATTLL
260 270 280 290 300
CRGSLAPLVP KCLPPQIIRE YGEQILDEIK ISTPKNMKKQ DSNRVRRALG
310 320 330 340 350
EANSASMQEE ERGRKCSHTE LESTGTTPAG NALERAARGN PESGNPQEHG
360 370 380 390 400
RHTSPASPHR PWWERHGPSS NVEALEKASI LTSSFTAEDD RGGSVIKYEE
410 420 430 440 450
NARRQWVREP PEALLSMLKD ADLSQAFQTY TIYRPGAEGF LKGPLSEDTA
460 470 480 490 500
SDSVDGDLDS VMLDPERFEP RLDEEDTDFE EDNENPDWVS ELKKHVGYGD
510
GPGGQLLGER A
Length:511
Mass (Da):57,464
Last modified:July 27, 2011 - v2
Checksum:i31E777B3BFA31930
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951K → N in AAD20986 (PubMed:10224116).Curated
Sequence conflicti95 – 951K → N in AAD16286 (PubMed:10393247).Curated
Sequence conflicti239 – 2391N → K in AAD16286 (PubMed:10393247).Curated
Sequence conflicti296 – 2961R → G in AAD20986 (PubMed:10224116).Curated
Sequence conflicti296 – 2961R → G in AAD16286 (PubMed:10393247).Curated
Sequence conflicti306 – 3061S → A in AAD20986 (PubMed:10224116).Curated
Sequence conflicti306 – 3061S → A in AAD16286 (PubMed:10393247).Curated
Sequence conflicti334 – 3341E → G in AAD20986 (PubMed:10224116).Curated
Sequence conflicti334 – 3341E → G in AAD16286 (PubMed:10393247).Curated
Sequence conflicti342 – 3432Missing in AAD16286 (PubMed:10393247).Curated
Sequence conflicti346 – 3461P → R in AAD20986 (PubMed:10224116).Curated
Sequence conflicti346 – 3461P → R in AAD16286 (PubMed:10393247).Curated
Sequence conflicti351 – 3511R → S in AAD20986 (PubMed:10224116).Curated
Sequence conflicti351 – 3511R → S in AAD16286 (PubMed:10393247).Curated
Sequence conflicti362 – 3621W → R in AAD20986 (PubMed:10224116).Curated
Sequence conflicti362 – 3621W → R in AAD16286 (PubMed:10393247).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093416 mRNA. Translation: AAD20986.1.
AF099066 mRNA. Translation: AAD16286.1.
AC117665 Genomic DNA. No translation available.
CCDSiCCDS52519.1.
RefSeqiNP_001156419.1. NM_001162947.1.
XP_006509174.1. XM_006509111.2.
UniGeneiMm.41413.

Genome annotation databases

EnsembliENSMUST00000110730; ENSMUSP00000106358; ENSMUSG00000031478.
ENSMUST00000178324; ENSMUSP00000136876; ENSMUSG00000031478.
GeneIDi23954.
KEGGimmu:23954.
UCSCiuc012gbb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093416 mRNA. Translation: AAD20986.1.
AF099066 mRNA. Translation: AAD16286.1.
AC117665 Genomic DNA. No translation available.
CCDSiCCDS52519.1.
RefSeqiNP_001156419.1. NM_001162947.1.
XP_006509174.1. XM_006509111.2.
UniGeneiMm.41413.

3D structure databases

ProteinModelPortaliQ9R0A5.
SMRiQ9R0A5. Positions 1-290, 457-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000106358.

PTM databases

PhosphoSiteiQ9R0A5.

Proteomic databases

MaxQBiQ9R0A5.
PaxDbiQ9R0A5.
PRIDEiQ9R0A5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110730; ENSMUSP00000106358; ENSMUSG00000031478.
ENSMUST00000178324; ENSMUSP00000136876; ENSMUSG00000031478.
GeneIDi23954.
KEGGimmu:23954.
UCSCiuc012gbb.1. mouse.

Organism-specific databases

CTDi4752.
MGIiMGI:1344371. Nek3.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000261617.
HOVERGENiHBG003063.
InParanoidiQ9R0A5.
KOiK08857.
OMAiRQWEKNV.

Miscellaneous databases

NextBioi303780.
PROiQ9R0A5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0A5.
ExpressionAtlasiQ9R0A5. baseline and differential.
GenevisibleiQ9R0A5. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators."
    Tanaka K., Nigg E.A.
    J. Biol. Chem. 274:13491-13497(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3."
    Chen A., Yanai A., Arama E., Kilfin G., Motro B.
    Gene 234:127-137(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons."
    Chang J., Baloh R.H., Milbrandt J.
    J. Cell Sci. 122:2274-2282(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, PHOSPHORYLATION AT THR-477, MUTAGENESIS OF THR-477.

Entry informationi

Entry nameiNEK3_MOUSE
AccessioniPrimary (citable) accession number: Q9R0A5
Secondary accession number(s): E9QLM2, Q9Z0X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.