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Q9R0A5

- NEK3_MOUSE

UniProt

Q9R0A5 - NEK3_MOUSE

Protein

Serine/threonine-protein kinase Nek3

Gene

Nek3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase Nek3 (EC:2.7.11.1)
    Alternative name(s):
    Never in mitosis A-related kinase 3
    Short name:
    NimA-related protein kinase 3
    Gene namesi
    Name:Nek3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1344371. Nek3.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell projectionaxon 1 Publication

    GO - Cellular componenti

    1. axon Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi477 – 4771T → A: Distorted neuronal morphology with disturbed polarity and deacetylation of microtubules. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 511511Serine/threonine-protein kinase Nek3PRO_0000086424Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei159 – 1591Phosphothreonine; by autocatalysisBy similarity
    Modified residuei477 – 4771Phosphothreonine3 Publications

    Post-translational modificationi

    Phosphorylation at Thr-477 regulates its catalytic activity.3 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9R0A5.
    PRIDEiQ9R0A5.

    PTM databases

    PhosphoSiteiQ9R0A5.

    Expressioni

    Tissue specificityi

    Brain.1 Publication

    Developmental stagei

    Highly expressed during embryogenesis and early postnatal life, but is expressed at lower levels in adults.1 Publication

    Gene expression databases

    ArrayExpressiQ9R0A5.
    BgeeiQ9R0A5.
    GenevestigatoriQ9R0A5.

    Interactioni

    Subunit structurei

    Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0A5.
    SMRiQ9R0A5. Positions 1-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 255252Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 282282Interaction with VAV2By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00720000108476.
    HOGENOMiHOG000261617.
    HOVERGENiHBG003063.
    InParanoidiQ9R0A5.
    KOiK08857.
    OMAiRQWEKNV.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R0A5-1 [UniParc]FASTAAdd to Basket

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    MDNYTVLRVI GQGSFGRALL VLQESSNQTF AMKEIRLLKS DTQTSRKEAV    50
    LLAKMKHPNI VAFKESFEAE GYLYIVMEYC DGGDLMQRIK QQKGKLFPED 100
    TILNWFIQIC LGVNHIHKRR VLHRDIKSKN VFLTHNGKVK LGDFGSARLL 150
    SSPMAFACTY VGTPYYVPPE IWENLPYNNK SDIWSLGCIL YELCALKHPF 200
    QANSWKNLIL KICQGPIHPL PALYSCKLQG LVKQMLKRNP SHRPSATTLL 250
    CRGSLAPLVP KCLPPQIIRE YGEQILDEIK ISTPKNMKKQ DSNRVRRALG 300
    EANSASMQEE ERGRKCSHTE LESTGTTPAG NALERAARGN PESGNPQEHG 350
    RHTSPASPHR PWWERHGPSS NVEALEKASI LTSSFTAEDD RGGSVIKYEE 400
    NARRQWVREP PEALLSMLKD ADLSQAFQTY TIYRPGAEGF LKGPLSEDTA 450
    SDSVDGDLDS VMLDPERFEP RLDEEDTDFE EDNENPDWVS ELKKHVGYGD 500
    GPGGQLLGER A 511
    Length:511
    Mass (Da):57,464
    Last modified:July 27, 2011 - v2
    Checksum:i31E777B3BFA31930
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 951K → N in AAD20986. (PubMed:10224116)Curated
    Sequence conflicti95 – 951K → N in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti239 – 2391N → K in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti296 – 2961R → G in AAD20986. (PubMed:10224116)Curated
    Sequence conflicti296 – 2961R → G in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti306 – 3061S → A in AAD20986. (PubMed:10224116)Curated
    Sequence conflicti306 – 3061S → A in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti334 – 3341E → G in AAD20986. (PubMed:10224116)Curated
    Sequence conflicti334 – 3341E → G in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti342 – 3432Missing in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti346 – 3461P → R in AAD20986. (PubMed:10224116)Curated
    Sequence conflicti346 – 3461P → R in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti351 – 3511R → S in AAD20986. (PubMed:10224116)Curated
    Sequence conflicti351 – 3511R → S in AAD16286. (PubMed:10393247)Curated
    Sequence conflicti362 – 3621W → R in AAD20986. (PubMed:10224116)Curated
    Sequence conflicti362 – 3621W → R in AAD16286. (PubMed:10393247)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093416 mRNA. Translation: AAD20986.1.
    AF099066 mRNA. Translation: AAD16286.1.
    AC117665 Genomic DNA. No translation available.
    CCDSiCCDS52519.1.
    RefSeqiNP_001156419.1. NM_001162947.1.
    XP_006509174.1. XM_006509111.1.
    UniGeneiMm.41413.

    Genome annotation databases

    EnsembliENSMUST00000110730; ENSMUSP00000106358; ENSMUSG00000031478.
    ENSMUST00000178324; ENSMUSP00000136876; ENSMUSG00000031478.
    GeneIDi23954.
    KEGGimmu:23954.
    UCSCiuc012gbb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093416 mRNA. Translation: AAD20986.1 .
    AF099066 mRNA. Translation: AAD16286.1 .
    AC117665 Genomic DNA. No translation available.
    CCDSi CCDS52519.1.
    RefSeqi NP_001156419.1. NM_001162947.1.
    XP_006509174.1. XM_006509111.1.
    UniGenei Mm.41413.

    3D structure databases

    ProteinModelPortali Q9R0A5.
    SMRi Q9R0A5. Positions 1-290.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9R0A5.

    Proteomic databases

    PaxDbi Q9R0A5.
    PRIDEi Q9R0A5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000110730 ; ENSMUSP00000106358 ; ENSMUSG00000031478 .
    ENSMUST00000178324 ; ENSMUSP00000136876 ; ENSMUSG00000031478 .
    GeneIDi 23954.
    KEGGi mmu:23954.
    UCSCi uc012gbb.1. mouse.

    Organism-specific databases

    CTDi 4752.
    MGIi MGI:1344371. Nek3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00720000108476.
    HOGENOMi HOG000261617.
    HOVERGENi HBG003063.
    InParanoidi Q9R0A5.
    KOi K08857.
    OMAi RQWEKNV.

    Miscellaneous databases

    NextBioi 303780.
    PROi Q9R0A5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R0A5.
    Bgeei Q9R0A5.
    Genevestigatori Q9R0A5.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators."
      Tanaka K., Nigg E.A.
      J. Biol. Chem. 274:13491-13497(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3."
      Chen A., Yanai A., Arama E., Kilfin G., Motro B.
      Gene 234:127-137(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons."
      Chang J., Baloh R.H., Milbrandt J.
      J. Cell Sci. 122:2274-2282(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, PHOSPHORYLATION AT THR-477, MUTAGENESIS OF THR-477.

    Entry informationi

    Entry nameiNEK3_MOUSE
    AccessioniPrimary (citable) accession number: Q9R0A5
    Secondary accession number(s): E9QLM2, Q9Z0X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3