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Q9R0A5

- NEK3_MOUSE

UniProt

Q9R0A5 - NEK3_MOUSE

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Protein
Serine/threonine-protein kinase Nek3
Gene
Nek3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATP By similarity
Active sitei125 – 1251Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek3 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 3
Short name:
NimA-related protein kinase 3
Gene namesi
Name:Nek3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1344371. Nek3.

Subcellular locationi

Cytoplasm. Cell projectionaxon 1 Publication

GO - Cellular componenti

  1. axon Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi477 – 4771T → A: Distorted neuronal morphology with disturbed polarity and deacetylation of microtubules. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Serine/threonine-protein kinase Nek3
PRO_0000086424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei159 – 1591Phosphothreonine; by autocatalysis By similarity
Modified residuei477 – 4771Phosphothreonine3 Publications

Post-translational modificationi

Phosphorylation at Thr-477 regulates its catalytic activity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9R0A5.
PRIDEiQ9R0A5.

PTM databases

PhosphoSiteiQ9R0A5.

Expressioni

Tissue specificityi

Brain.1 Publication

Developmental stagei

Highly expressed during embryogenesis and early postnatal life, but is expressed at lower levels in adults.1 Publication

Gene expression databases

ArrayExpressiQ9R0A5.
BgeeiQ9R0A5.
GenevestigatoriQ9R0A5.

Interactioni

Subunit structurei

Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9R0A5.
SMRiQ9R0A5. Positions 1-290.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 255252Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 282282Interaction with VAV2 By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00720000108476.
HOGENOMiHOG000261617.
HOVERGENiHBG003063.
InParanoidiQ9R0A5.
KOiK08857.
OMAiRQWEKNV.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0A5-1 [UniParc]FASTAAdd to Basket

« Hide

MDNYTVLRVI GQGSFGRALL VLQESSNQTF AMKEIRLLKS DTQTSRKEAV    50
LLAKMKHPNI VAFKESFEAE GYLYIVMEYC DGGDLMQRIK QQKGKLFPED 100
TILNWFIQIC LGVNHIHKRR VLHRDIKSKN VFLTHNGKVK LGDFGSARLL 150
SSPMAFACTY VGTPYYVPPE IWENLPYNNK SDIWSLGCIL YELCALKHPF 200
QANSWKNLIL KICQGPIHPL PALYSCKLQG LVKQMLKRNP SHRPSATTLL 250
CRGSLAPLVP KCLPPQIIRE YGEQILDEIK ISTPKNMKKQ DSNRVRRALG 300
EANSASMQEE ERGRKCSHTE LESTGTTPAG NALERAARGN PESGNPQEHG 350
RHTSPASPHR PWWERHGPSS NVEALEKASI LTSSFTAEDD RGGSVIKYEE 400
NARRQWVREP PEALLSMLKD ADLSQAFQTY TIYRPGAEGF LKGPLSEDTA 450
SDSVDGDLDS VMLDPERFEP RLDEEDTDFE EDNENPDWVS ELKKHVGYGD 500
GPGGQLLGER A 511
Length:511
Mass (Da):57,464
Last modified:July 27, 2011 - v2
Checksum:i31E777B3BFA31930
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951K → N in AAD20986. 1 Publication
Sequence conflicti95 – 951K → N in AAD16286. 1 Publication
Sequence conflicti239 – 2391N → K in AAD16286. 1 Publication
Sequence conflicti296 – 2961R → G in AAD20986. 1 Publication
Sequence conflicti296 – 2961R → G in AAD16286. 1 Publication
Sequence conflicti306 – 3061S → A in AAD20986. 1 Publication
Sequence conflicti306 – 3061S → A in AAD16286. 1 Publication
Sequence conflicti334 – 3341E → G in AAD20986. 1 Publication
Sequence conflicti334 – 3341E → G in AAD16286. 1 Publication
Sequence conflicti342 – 3432Missing in AAD16286. 1 Publication
Sequence conflicti346 – 3461P → R in AAD20986. 1 Publication
Sequence conflicti346 – 3461P → R in AAD16286. 1 Publication
Sequence conflicti351 – 3511R → S in AAD20986. 1 Publication
Sequence conflicti351 – 3511R → S in AAD16286. 1 Publication
Sequence conflicti362 – 3621W → R in AAD20986. 1 Publication
Sequence conflicti362 – 3621W → R in AAD16286. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093416 mRNA. Translation: AAD20986.1.
AF099066 mRNA. Translation: AAD16286.1.
AC117665 Genomic DNA. No translation available.
CCDSiCCDS52519.1.
RefSeqiNP_001156419.1. NM_001162947.1.
XP_006509174.1. XM_006509111.1.
UniGeneiMm.41413.

Genome annotation databases

EnsembliENSMUST00000110730; ENSMUSP00000106358; ENSMUSG00000031478.
ENSMUST00000178324; ENSMUSP00000136876; ENSMUSG00000031478.
GeneIDi23954.
KEGGimmu:23954.
UCSCiuc012gbb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093416 mRNA. Translation: AAD20986.1 .
AF099066 mRNA. Translation: AAD16286.1 .
AC117665 Genomic DNA. No translation available.
CCDSi CCDS52519.1.
RefSeqi NP_001156419.1. NM_001162947.1.
XP_006509174.1. XM_006509111.1.
UniGenei Mm.41413.

3D structure databases

ProteinModelPortali Q9R0A5.
SMRi Q9R0A5. Positions 1-290.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9R0A5.

Proteomic databases

PaxDbi Q9R0A5.
PRIDEi Q9R0A5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000110730 ; ENSMUSP00000106358 ; ENSMUSG00000031478 .
ENSMUST00000178324 ; ENSMUSP00000136876 ; ENSMUSG00000031478 .
GeneIDi 23954.
KEGGi mmu:23954.
UCSCi uc012gbb.1. mouse.

Organism-specific databases

CTDi 4752.
MGIi MGI:1344371. Nek3.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00720000108476.
HOGENOMi HOG000261617.
HOVERGENi HBG003063.
InParanoidi Q9R0A5.
KOi K08857.
OMAi RQWEKNV.

Miscellaneous databases

NextBioi 303780.
PROi Q9R0A5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9R0A5.
Bgeei Q9R0A5.
Genevestigatori Q9R0A5.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators."
    Tanaka K., Nigg E.A.
    J. Biol. Chem. 274:13491-13497(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3."
    Chen A., Yanai A., Arama E., Kilfin G., Motro B.
    Gene 234:127-137(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons."
    Chang J., Baloh R.H., Milbrandt J.
    J. Cell Sci. 122:2274-2282(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, PHOSPHORYLATION AT THR-477, MUTAGENESIS OF THR-477.

Entry informationi

Entry nameiNEK3_MOUSE
AccessioniPrimary (citable) accession number: Q9R0A5
Secondary accession number(s): E9QLM2, Q9Z0X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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