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Protein

Chloride channel protein 2

Gene

Clcn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei170ChlorideBy similarity1
Binding sitei467Chloride; via amide nitrogenBy similarity1
Binding sitei561ChlorideBy similarity1

GO - Molecular functioni

  • voltage-gated chloride channel activity Source: MGI

GO - Biological processi

  • cell differentiation involved in salivary gland development Source: MGI
  • retina development in camera-type eye Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 2
Short name:
ClC-2
Gene namesi
Name:Clcn2
Synonyms:Clc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:105061. Clcn2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 95CytoplasmicBy similarityAdd BLAST95
Transmembranei96 – 129HelicalBy similarityAdd BLAST34
Transmembranei138 – 163HelicalBy similarityAdd BLAST26
Intramembranei172 – 179HelicalSequence analysis8
Transmembranei188 – 206HelicalBy similarityAdd BLAST19
Transmembranei213 – 231HelicalBy similarityAdd BLAST19
Intramembranei247 – 259HelicalBy similarityAdd BLAST13
Intramembranei263 – 271HelicalBy similarity9
Transmembranei283 – 303HelicalBy similarityAdd BLAST21
Transmembranei329 – 357HelicalBy similarityAdd BLAST29
Transmembranei366 – 385HelicalBy similarityAdd BLAST20
Transmembranei437 – 457HelicalBy similarityAdd BLAST21
Transmembranei465 – 488HelicalBy similarityAdd BLAST24
Intramembranei505 – 519HelicalBy similarityAdd BLAST15
Intramembranei520 – 521Note=Loop between two helicesBy similarity2
Intramembranei522 – 533HelicalBy similarityAdd BLAST12
Intramembranei534 – 538Note=Loop between two helicesBy similarity5
Transmembranei539 – 556HelicalBy similarityAdd BLAST18
Topological domaini557 – 908CytoplasmicBy similarityAdd BLAST352

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944341 – 908Chloride channel protein 2Add BLAST908

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphothreonineCombined sources1
Modified residuei768PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Activated by dephosphorylation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9R0A1.
PaxDbiQ9R0A1.
PRIDEiQ9R0A1.

PTM databases

iPTMnetiQ9R0A1.
PhosphoSitePlusiQ9R0A1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022843.
CleanExiMM_CLCN2.
ExpressionAtlasiQ9R0A1. baseline and differential.
GenevisibleiQ9R0A1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007207.

Structurei

3D structure databases

ProteinModelPortaliQ9R0A1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini592 – 650CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini800 – 860CBS 2PROSITE-ProRule annotationAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi169 – 173Selectivity filter part_1By similarity5
Motifi211 – 215Selectivity filter part_2By similarity5
Motifi465 – 469Selectivity filter part_3By similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 14Poly-AlaAdd BLAST13

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9R0A1.
KOiK05011.
OMAiESCEKRK.
OrthoDBiEOG091G01RJ.
TreeFamiTF300522.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR002244. Cl-channel-2.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01113. CLCHANNEL2.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATAAAAA AAAAGEGMEP RALQYEQTLM YGRYTQELGA FAKEEAARIR
60 70 80 90 100
LGGPEPWKGS PSARATPELL EYGQSRCARC RICSVRCHKF LVSRVGEDWI
110 120 130 140 150
FLVLLGLLMA LVSWAMDYAI AVCLQAQQWM SRGLNTNILL QYLAWVTYPV
160 170 180 190 200
VLITFSAGFT QILAPQAVGS GIPEMKTILR GVVLKEYLTL KTFVAKVIGL
210 220 230 240 250
TCALGSGMPL GKEGPFVHIA SMCAALLSKF LSLFGGIYEH ESRNTEMLAA
260 270 280 290 300
ACAVGVGCCF AAPIGGVLFS IEVTSTFFAV RNYWRGFFAA TFSAFIFRVL
310 320 330 340 350
AVWNRDEETI TALFKTRFRL DFPFDLQELP AFAVIGIASG FGGALFVYLN
360 370 380 390 400
RKIVQVMRKQ KTINRFLMRK RLLFPALVTL LISTLTFPPG FGQFMAGQLS
410 420 430 440 450
QKETLVTLFD NRTWVRQGLV EDLELPSTSQ AWSPPRANVF LTLVIFILMK
460 470 480 490 500
FWMSALATTI PVPCGAFMPV FVIGAAFGRL VGESMAAWFP DGIHTDSSTY
510 520 530 540 550
RIVPGGYAVV GAAALAGAVT HTVSTAVIVF ELTGQIAHIL PVMIAVILAN
560 570 580 590 600
AVAQSLQPSL YDSIIRIKKL PYLPELGWGR HQQYRVRVED IMVRDVPHVA
610 620 630 640 650
LSCTFRDLRL ALHRTKGRML ALVESPESMI LLGSIERSQV VALLGAQLSP
660 670 680 690 700
ARRRQHMQKL RKAQLSPPSD QESPPSSETS IRFQVNTEDS GFSGAHGQTH
710 720 730 740 750
KPLKPALKRG PSNSTSLQEG TTGNMESAGI ALRSLFCGSP PLEATSELEK
760 770 780 790 800
SESCDKRKLK RVRISLASDS DPEAEMSPEE ILEWEEQQLD EPVNFSDCKI
810 820 830 840 850
DPAPFQLVER TSLHKTHTIF SLLGVDHAYV TSIGRLIGIV TLKELRKAIE
860 870 880 890 900
GSVTAQGVKV RPPLASFRDS ATSSSDTETT EVHALWGPRS RHGLPREGTP

SDSDDKCQ
Length:908
Mass (Da):99,447
Last modified:July 27, 2011 - v2
Checksum:i6CF58033B60CA578
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti667P → S in AAD50604 (PubMed:10524221).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097415 mRNA. Translation: AAD50604.1.
AF139724 mRNA. Translation: AAD26466.1.
CT010490, AC087898 Genomic DNA. Translation: CAP19214.1.
BC120699 mRNA. Translation: AAI20700.1.
BC137625 mRNA. Translation: AAI37626.1.
CCDSiCCDS28057.1.
RefSeqiNP_034030.2. NM_009900.2.
UniGeneiMm.177761.

Genome annotation databases

EnsembliENSMUST00000007207; ENSMUSP00000007207; ENSMUSG00000022843.
GeneIDi12724.
KEGGimmu:12724.
UCSCiuc012ada.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097415 mRNA. Translation: AAD50604.1.
AF139724 mRNA. Translation: AAD26466.1.
CT010490, AC087898 Genomic DNA. Translation: CAP19214.1.
BC120699 mRNA. Translation: AAI20700.1.
BC137625 mRNA. Translation: AAI37626.1.
CCDSiCCDS28057.1.
RefSeqiNP_034030.2. NM_009900.2.
UniGeneiMm.177761.

3D structure databases

ProteinModelPortaliQ9R0A1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007207.

PTM databases

iPTMnetiQ9R0A1.
PhosphoSitePlusiQ9R0A1.

Proteomic databases

MaxQBiQ9R0A1.
PaxDbiQ9R0A1.
PRIDEiQ9R0A1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007207; ENSMUSP00000007207; ENSMUSG00000022843.
GeneIDi12724.
KEGGimmu:12724.
UCSCiuc012ada.1. mouse.

Organism-specific databases

CTDi1181.
MGIiMGI:105061. Clcn2.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9R0A1.
KOiK05011.
OMAiESCEKRK.
OrthoDBiEOG091G01RJ.
TreeFamiTF300522.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ9R0A1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022843.
CleanExiMM_CLCN2.
ExpressionAtlasiQ9R0A1. baseline and differential.
GenevisibleiQ9R0A1. MM.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR002244. Cl-channel-2.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01113. CLCHANNEL2.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0A1
Secondary accession number(s): Q0VBC2, Q9WUJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.