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Protein

Chloride channel protein 2

Gene

Clcn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei170 – 1701ChlorideBy similarity
Binding sitei467 – 4671Chloride; via amide nitrogenBy similarity
Binding sitei561 – 5611ChlorideBy similarity

GO - Molecular functioni

  • voltage-gated chloride channel activity Source: MGI

GO - Biological processi

  • cell differentiation involved in salivary gland development Source: MGI
  • retina development in camera-type eye Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 2
Short name:
ClC-2
Gene namesi
Name:Clcn2
Synonyms:Clc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:105061. Clcn2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9595CytoplasmicBy similarityAdd
BLAST
Transmembranei96 – 12934HelicalBy similarityAdd
BLAST
Transmembranei138 – 16326HelicalBy similarityAdd
BLAST
Intramembranei172 – 1798HelicalSequence analysis
Transmembranei188 – 20619HelicalBy similarityAdd
BLAST
Transmembranei213 – 23119HelicalBy similarityAdd
BLAST
Intramembranei247 – 25913HelicalBy similarityAdd
BLAST
Intramembranei263 – 2719HelicalBy similarity
Transmembranei283 – 30321HelicalBy similarityAdd
BLAST
Transmembranei329 – 35729HelicalBy similarityAdd
BLAST
Transmembranei366 – 38520HelicalBy similarityAdd
BLAST
Transmembranei437 – 45721HelicalBy similarityAdd
BLAST
Transmembranei465 – 48824HelicalBy similarityAdd
BLAST
Intramembranei505 – 51915HelicalBy similarityAdd
BLAST
Intramembranei520 – 5212Note=Loop between two helicesBy similarity
Intramembranei522 – 53312HelicalBy similarityAdd
BLAST
Intramembranei534 – 5385Note=Loop between two helicesBy similarity
Transmembranei539 – 55618HelicalBy similarityAdd
BLAST
Topological domaini557 – 908352CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908Chloride channel protein 2PRO_0000094434Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281PhosphothreonineCombined sources
Modified residuei768 – 7681PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. Activated by dephosphorylation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9R0A1.
PaxDbiQ9R0A1.
PRIDEiQ9R0A1.

PTM databases

iPTMnetiQ9R0A1.
PhosphoSiteiQ9R0A1.

Expressioni

Gene expression databases

BgeeiQ9R0A1.
CleanExiMM_CLCN2.
ExpressionAtlasiQ9R0A1. baseline and differential.
GenevisibleiQ9R0A1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007207.

Structurei

3D structure databases

ProteinModelPortaliQ9R0A1.
SMRiQ9R0A1. Positions 101-659, 776-851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini592 – 65059CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini800 – 86061CBS 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi169 – 1735Selectivity filter part_1By similarity
Motifi211 – 2155Selectivity filter part_2By similarity
Motifi465 – 4695Selectivity filter part_3By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 1413Poly-AlaAdd
BLAST

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9R0A1.
KOiK05011.
OMAiESCEKRK.
OrthoDBiEOG77WWCD.
TreeFamiTF300522.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR002244. Cl-channel-2.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01113. CLCHANNEL2.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATAAAAA AAAAGEGMEP RALQYEQTLM YGRYTQELGA FAKEEAARIR
60 70 80 90 100
LGGPEPWKGS PSARATPELL EYGQSRCARC RICSVRCHKF LVSRVGEDWI
110 120 130 140 150
FLVLLGLLMA LVSWAMDYAI AVCLQAQQWM SRGLNTNILL QYLAWVTYPV
160 170 180 190 200
VLITFSAGFT QILAPQAVGS GIPEMKTILR GVVLKEYLTL KTFVAKVIGL
210 220 230 240 250
TCALGSGMPL GKEGPFVHIA SMCAALLSKF LSLFGGIYEH ESRNTEMLAA
260 270 280 290 300
ACAVGVGCCF AAPIGGVLFS IEVTSTFFAV RNYWRGFFAA TFSAFIFRVL
310 320 330 340 350
AVWNRDEETI TALFKTRFRL DFPFDLQELP AFAVIGIASG FGGALFVYLN
360 370 380 390 400
RKIVQVMRKQ KTINRFLMRK RLLFPALVTL LISTLTFPPG FGQFMAGQLS
410 420 430 440 450
QKETLVTLFD NRTWVRQGLV EDLELPSTSQ AWSPPRANVF LTLVIFILMK
460 470 480 490 500
FWMSALATTI PVPCGAFMPV FVIGAAFGRL VGESMAAWFP DGIHTDSSTY
510 520 530 540 550
RIVPGGYAVV GAAALAGAVT HTVSTAVIVF ELTGQIAHIL PVMIAVILAN
560 570 580 590 600
AVAQSLQPSL YDSIIRIKKL PYLPELGWGR HQQYRVRVED IMVRDVPHVA
610 620 630 640 650
LSCTFRDLRL ALHRTKGRML ALVESPESMI LLGSIERSQV VALLGAQLSP
660 670 680 690 700
ARRRQHMQKL RKAQLSPPSD QESPPSSETS IRFQVNTEDS GFSGAHGQTH
710 720 730 740 750
KPLKPALKRG PSNSTSLQEG TTGNMESAGI ALRSLFCGSP PLEATSELEK
760 770 780 790 800
SESCDKRKLK RVRISLASDS DPEAEMSPEE ILEWEEQQLD EPVNFSDCKI
810 820 830 840 850
DPAPFQLVER TSLHKTHTIF SLLGVDHAYV TSIGRLIGIV TLKELRKAIE
860 870 880 890 900
GSVTAQGVKV RPPLASFRDS ATSSSDTETT EVHALWGPRS RHGLPREGTP

SDSDDKCQ
Length:908
Mass (Da):99,447
Last modified:July 27, 2011 - v2
Checksum:i6CF58033B60CA578
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti667 – 6671P → S in AAD50604 (PubMed:10524221).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097415 mRNA. Translation: AAD50604.1.
AF139724 mRNA. Translation: AAD26466.1.
CT010490, AC087898 Genomic DNA. Translation: CAP19214.1.
BC120699 mRNA. Translation: AAI20700.1.
BC137625 mRNA. Translation: AAI37626.1.
CCDSiCCDS28057.1.
RefSeqiNP_034030.2. NM_009900.2.
UniGeneiMm.177761.

Genome annotation databases

EnsembliENSMUST00000007207; ENSMUSP00000007207; ENSMUSG00000022843.
GeneIDi12724.
KEGGimmu:12724.
UCSCiuc012ada.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097415 mRNA. Translation: AAD50604.1.
AF139724 mRNA. Translation: AAD26466.1.
CT010490, AC087898 Genomic DNA. Translation: CAP19214.1.
BC120699 mRNA. Translation: AAI20700.1.
BC137625 mRNA. Translation: AAI37626.1.
CCDSiCCDS28057.1.
RefSeqiNP_034030.2. NM_009900.2.
UniGeneiMm.177761.

3D structure databases

ProteinModelPortaliQ9R0A1.
SMRiQ9R0A1. Positions 101-659, 776-851.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007207.

PTM databases

iPTMnetiQ9R0A1.
PhosphoSiteiQ9R0A1.

Proteomic databases

MaxQBiQ9R0A1.
PaxDbiQ9R0A1.
PRIDEiQ9R0A1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007207; ENSMUSP00000007207; ENSMUSG00000022843.
GeneIDi12724.
KEGGimmu:12724.
UCSCiuc012ada.1. mouse.

Organism-specific databases

CTDi1181.
MGIiMGI:105061. Clcn2.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9R0A1.
KOiK05011.
OMAiESCEKRK.
OrthoDBiEOG77WWCD.
TreeFamiTF300522.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ9R0A1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0A1.
CleanExiMM_CLCN2.
ExpressionAtlasiQ9R0A1. baseline and differential.
GenevisibleiQ9R0A1. MM.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR002244. Cl-channel-2.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01113. CLCHANNEL2.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of ClC-2 chloride channel from murine duodenum and its presence in CFTR knockout mice."
    Joo N.S., Clarke L.L., Han B.H., Forte L.R., Kim H.D.
    Biochim. Biophys. Acta 1446:431-437(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Duodenal mucosa.
  2. "CFTR and ClC-2 expression levels in the fetal mouse lung."
    Hathaway J.K., Rice W.R., Wert S., Whitsett J.A.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: FVB/N.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiCLCN2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0A1
Secondary accession number(s): Q0VBC2, Q9WUJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.