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Q9R098

- HGFA_MOUSE

UniProt

Q9R098 - HGFA_MOUSE

Protein

Hepatocyte growth factor activator

Gene

Hgfac

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei445 – 4451Charge relay systemBy similarity
    Active sitei495 – 4951Charge relay systemBy similarity
    Active sitei596 – 5961Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.228.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hepatocyte growth factor activator (EC:3.4.21.-)
    Short name:
    HGF activator
    Short name:
    HGFA
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Hgfac
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1859281. Hgfac.

    Subcellular locationi

    Secreted By similarity
    Note: Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. extracellular space Source: InterPro
    3. rough endoplasmic reticulum Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434By similarityAdd
    BLAST
    Propeptidei35 – 369335Removed in mature formBy similarityPRO_0000027914Add
    BLAST
    Chaini370 – 40536Hepatocyte growth factor activator short chainPRO_0000027915Add
    BLAST
    Chaini406 – 653248Hepatocyte growth factor activator long chainPRO_0000027916Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi105 ↔ 130By similarity
    Disulfide bondi119 ↔ 145By similarity
    Disulfide bondi161 ↔ 172By similarity
    Disulfide bondi166 ↔ 183By similarity
    Disulfide bondi185 ↔ 194By similarity
    Disulfide bondi199 ↔ 227By similarity
    Disulfide bondi225 ↔ 234By similarity
    Disulfide bondi242 ↔ 253By similarity
    Disulfide bondi247 ↔ 264By similarity
    Disulfide bondi266 ↔ 275By similarity
    Disulfide bondi283 ↔ 364By similarity
    Glycosylationi287 – 2871N-linked (GlcNAc...)1 Publication
    Disulfide bondi304 ↔ 346By similarity
    Disulfide bondi335 ↔ 359By similarity
    Disulfide bondi392 ↔ 519Interchain (between short and long chains)PROSITE-ProRule annotation
    Disulfide bondi430 ↔ 446By similarity
    Disulfide bondi438 ↔ 508By similarity
    Glycosylationi466 – 4661N-linked (GlcNAc...)1 Publication
    Disulfide bondi533 ↔ 602By similarity
    Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi565 ↔ 581By similarity
    Disulfide bondi592 ↔ 620By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ9R098.
    PRIDEiQ9R098.

    PTM databases

    PhosphoSiteiQ9R098.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9R098.
    BgeeiQ9R098.
    CleanExiMM_HGFAC.
    GenevestigatoriQ9R098.

    Interactioni

    Subunit structurei

    Heterodimer of a short chain and a long chain linked by a disulfide bond.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R098.
    SMRiQ9R098. Positions 101-644.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini100 – 14748Fibronectin type-IIPROSITE-ProRule annotationAdd
    BLAST
    Domaini157 – 19539EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini197 – 23741Fibronectin type-IPROSITE-ProRule annotationAdd
    BLAST
    Domaini238 – 27639EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini283 – 36482KringlePROSITE-ProRule annotationAdd
    BLAST
    Domaini406 – 644239Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 fibronectin type-I domain.PROSITE-ProRule annotation
    Contains 1 fibronectin type-II domain.PROSITE-ProRule annotation
    Contains 1 kringle domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Kringle, Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115235.
    HOGENOMiHOG000237314.
    HOVERGENiHBG004345.
    InParanoidiQ9R098.
    KOiK09631.
    OMAiGTRHTAC.
    OrthoDBiEOG75B84T.
    PhylomeDBiQ9R098.
    TreeFamiTF329901.

    Family and domain databases

    Gene3Di2.10.10.10. 1 hit.
    2.40.20.10. 1 hit.
    InterProiIPR014394. Coagulation_fac_XIIa/HGFA.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000083. Fibronectin_type1.
    IPR000562. FN_type2_col-bd.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00008. EGF. 2 hits.
    PF00039. fn1. 1 hit.
    PF00040. fn2. 1 hit.
    PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001146. Factor_XII_HGFA. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00181. EGF. 2 hits.
    SM00059. FN2. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 2 hits.
    PROSITEiPS00022. EGF_1. 2 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01253. FN1_1. 1 hit.
    PS51091. FN1_2. 1 hit.
    PS00023. FN2_1. 1 hit.
    PS51092. FN2_2. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9R098-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRQAWISSL CPLPRPCPFL LLLLLLVVPR GAQPQAGRNH TEPPGPNVTA    50
    TPVTPTIPVI SGNVSTSTES APAAETEGPQ SERYPPPSSS SPPGGQVLTE 100
    SGQPCRFPFR YGGRMLHSCT SEGSAYRKWC ATTHNYDRDR AWGYCAEVTL 150
    PVEGPAILDP CASGPCLNGG TCSSTHDHGS YHCSCPLAFT GKDCGTEKCF 200
    DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHT ACLSSPCLNG 250
    GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAAS 300
    GLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK 350
    DNALSWEYCR LTACESLARV HSQTPEILAA LPESAPAVRP TCGKRHKKRT 400
    FLRPRIIGGS SSLPGSHPWL AAIYIGNSFC AGSLVHTCWV VSAAHCFANS 450
    PPRDSITVVL GQHFFNRTTD VTQTFGIEKY VPYTLYSVFN PNNHDLVLIR 500
    LKKKGERCAV RSQFVQPICL PEAGSSFPTG HKCQIAGWGH MDENVSSYSN 550
    SLLEALVPLV ADHKCSSPEV YGADISPNML CAGYFDCKSD ACQGDSGGPL 600
    VCEKNGVAYL YGIISWGDGC GRLNKPGVYT RVANYVDWIN DRIRPPKRPV 650
    ATS 653
    Length:653
    Mass (Da):70,568
    Last modified:May 1, 2000 - v1
    Checksum:i88B4B20255DF7FDC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1641G → W in AAF34712. (PubMed:11032833)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099017 mRNA. Translation: AAF02489.1.
    AF224724 mRNA. Translation: AAF34712.1.
    CCDSiCCDS19223.1.
    RefSeqiNP_062320.2. NM_019447.2.
    UniGeneiMm.27369.

    Genome annotation databases

    EnsembliENSMUST00000030985; ENSMUSP00000030985; ENSMUSG00000029102.
    GeneIDi54426.
    KEGGimmu:54426.
    UCSCiuc008xdj.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099017 mRNA. Translation: AAF02489.1 .
    AF224724 mRNA. Translation: AAF34712.1 .
    CCDSi CCDS19223.1.
    RefSeqi NP_062320.2. NM_019447.2.
    UniGenei Mm.27369.

    3D structure databases

    ProteinModelPortali Q9R098.
    SMRi Q9R098. Positions 101-644.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.228.

    PTM databases

    PhosphoSitei Q9R098.

    Proteomic databases

    PaxDbi Q9R098.
    PRIDEi Q9R098.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030985 ; ENSMUSP00000030985 ; ENSMUSG00000029102 .
    GeneIDi 54426.
    KEGGi mmu:54426.
    UCSCi uc008xdj.2. mouse.

    Organism-specific databases

    CTDi 3083.
    MGIi MGI:1859281. Hgfac.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115235.
    HOGENOMi HOG000237314.
    HOVERGENi HBG004345.
    InParanoidi Q9R098.
    KOi K09631.
    OMAi GTRHTAC.
    OrthoDBi EOG75B84T.
    PhylomeDBi Q9R098.
    TreeFami TF329901.

    Miscellaneous databases

    NextBioi 311300.
    PROi Q9R098.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R098.
    Bgeei Q9R098.
    CleanExi MM_HGFAC.
    Genevestigatori Q9R098.

    Family and domain databases

    Gene3Di 2.10.10.10. 1 hit.
    2.40.20.10. 1 hit.
    InterProi IPR014394. Coagulation_fac_XIIa/HGFA.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000083. Fibronectin_type1.
    IPR000562. FN_type2_col-bd.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00008. EGF. 2 hits.
    PF00039. fn1. 1 hit.
    PF00040. fn2. 1 hit.
    PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001146. Factor_XII_HGFA. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00181. EGF. 2 hits.
    SM00059. FN2. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 2 hits.
    PROSITEi PS00022. EGF_1. 2 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01253. FN1_1. 1 hit.
    PS51091. FN1_2. 1 hit.
    PS00023. FN2_1. 1 hit.
    PS51092. FN2_2. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse hepatocyte growth factor activator gene: its expression not only in the liver but also in the gastrointestinal tract."
      Itoh H., Hamasuna R., Kataoka H., Yamauchi M., Miyazawa K., Kitamura N., Koono M.
      Biochim. Biophys. Acta 1491:295-302(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Activation of hepatocyte growth factor (HGF) by endogenous HGF activator is required for metanephric kidney morphogenesis in vitro."
      van Adelsberg J.S., Sehgal S., Kukes A., Brady C., Barasch J., Yang J., Huan Y.
      J. Biol. Chem. 276:15099-15106(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-466.
      Strain: C57BL/6.
      Tissue: Plasma.
    4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-287.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiHGFA_MOUSE
    AccessioniPrimary (citable) accession number: Q9R098
    Secondary accession number(s): Q9JKV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3