Hepatocyte growth factor activator precursor

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Q9R098 (HGFA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Hepatocyte growth factor activator
Short name=HGF activator
Short name=HGFA
EC=3.4.21.-

Cleaved into the following 2 chains:

Hepatocyte growth factor activator short chain
Hepatocyte growth factor activator long chain

Gene names

Name:

Hgfac

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form By similarity.
Subunit structure	Heterodimer of a short chain and a long chain linked by a disulfide bond By similarity.
Subcellular location	Secreted By similarity. Note: Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 2 EGF-like domains. Contains 1 fibronectin type-I domain. Contains 1 fibronectin type-II domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Kringle Repeat Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: InterPro rough endoplasmic reticulum Inferred from direct assay PubMed 12923239. Source: MGI
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 34

By similarity

Propeptide

35 – 369

335

Removed in mature form By similarity

PRO_0000027914

Chain

370 – 405

Hepatocyte growth factor activator short chain

PRO_0000027915

Chain

406 – 653

248

Hepatocyte growth factor activator long chain

PRO_0000027916

Regions

Domain

100 – 147

Fibronectin type-II

Domain

157 – 195

EGF-like 1

Domain

197 – 237

Fibronectin type-I

Domain

238 – 276

EGF-like 2

Domain

283 – 364

Kringle

Domain

406 – 644

239

Peptidase S1

Sites

Active site

445

Charge relay system By similarity

Active site

495

Charge relay system By similarity

Active site

596

Charge relay system By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

287

N-linked (GlcNAc...) Ref.4

Glycosylation

466

N-linked (GlcNAc...) Ref.3

Glycosylation

544

N-linked (GlcNAc...) Potential

Disulfide bond

105 ↔ 130

By similarity

Disulfide bond

119 ↔ 145

By similarity

Disulfide bond

161 ↔ 172

By similarity

Disulfide bond

166 ↔ 183

By similarity

Disulfide bond

185 ↔ 194

By similarity

Disulfide bond

199 ↔ 227

By similarity

Disulfide bond

225 ↔ 234

By similarity

Disulfide bond

242 ↔ 253

By similarity

Disulfide bond

247 ↔ 264

By similarity

Disulfide bond

266 ↔ 275

By similarity

Disulfide bond

283 ↔ 364

By similarity

Disulfide bond

304 ↔ 346

By similarity

Disulfide bond

335 ↔ 359

By similarity

Disulfide bond

392 ↔ 519

Interchain (between short and long chains) By similarity

Disulfide bond

430 ↔ 446

By similarity

Disulfide bond

438 ↔ 508

By similarity

Disulfide bond

533 ↔ 602

By similarity

Disulfide bond

565 ↔ 581

By similarity

Disulfide bond

592 ↔ 620

By similarity

Experimental info

Sequence conflict

164

G → W in AAF34712. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q9R098 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 88B4B20255DF7FDC
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FASTA

653

70,568

        10         20         30         40         50         60 
MGRQAWISSL CPLPRPCPFL LLLLLLVVPR GAQPQAGRNH TEPPGPNVTA TPVTPTIPVI 

        70         80         90        100        110        120 
SGNVSTSTES APAAETEGPQ SERYPPPSSS SPPGGQVLTE SGQPCRFPFR YGGRMLHSCT 

       130        140        150        160        170        180 
SEGSAYRKWC ATTHNYDRDR AWGYCAEVTL PVEGPAILDP CASGPCLNGG TCSSTHDHGS 

       190        200        210        220        230        240 
YHCSCPLAFT GKDCGTEKCF DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHT 

       250        260        270        280        290        300 
ACLSSPCLNG GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAAS 

       310        320        330        340        350        360 
GLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK DNALSWEYCR 

       370        380        390        400        410        420 
LTACESLARV HSQTPEILAA LPESAPAVRP TCGKRHKKRT FLRPRIIGGS SSLPGSHPWL 

       430        440        450        460        470        480 
AAIYIGNSFC AGSLVHTCWV VSAAHCFANS PPRDSITVVL GQHFFNRTTD VTQTFGIEKY 

       490        500        510        520        530        540 
VPYTLYSVFN PNNHDLVLIR LKKKGERCAV RSQFVQPICL PEAGSSFPTG HKCQIAGWGH 

       550        560        570        580        590        600 
MDENVSSYSN SLLEALVPLV ADHKCSSPEV YGADISPNML CAGYFDCKSD ACQGDSGGPL 

       610        620        630        640        650 
VCEKNGVAYL YGIISWGDGC GRLNKPGVYT RVANYVDWIN DRIRPPKRPV ATS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse hepatocyte growth factor activator gene: its expression not only in the liver but also in the gastrointestinal tract." Itoh H., Hamasuna R., Kataoka H., Yamauchi M., Miyazawa K., Kitamura N., Koono M. Biochim. Biophys. Acta 1491:295-302(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Activation of hepatocyte growth factor (HGF) by endogenous HGF activator is required for metanephric kidney morphogenesis in vitro." van Adelsberg J.S., Sehgal S., Kukes A., Brady C., Barasch J., Yang J., Huan Y. J. Biol. Chem. 276:15099-15106(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-466, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[4]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-287, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF099017 mRNA. Translation: AAF02489.1. AF224724 mRNA. Translation: AAF34712.1.
IPI	IPI00227857.
RefSeq	NP_062320.2. NM_019447.2.
UniGene	Mm.27369.
3D structure databases
ProteinModelPortal	Q9R098.
SMR	Q9R098. Positions 101-644.
ModBase	Search...
Protein family/group databases
MEROPS	S01.228.
PTM databases
PhosphoSite	Q9R098.
Proteomic databases
PaxDb	Q9R098.
PRIDE	Q9R098.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030985; ENSMUSP00000030985; ENSMUSG00000029102.
GeneID	54426.
KEGG	mmu:54426.
Organism-specific databases
CTD	3083.
MGI	MGI:1859281. Hgfac.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00570000078945.
HOGENOM	HOG000237314.
HOVERGEN	HBG004345.
InParanoid	Q9R098.
KO	K09631.
OMA	RDRAWGY.
OrthoDB	EOG4PK27Q.
Gene expression databases
ArrayExpress	Q9R098.
Bgee	Q9R098.
CleanEx	MM_HGFAC.
Genevestigator	Q9R098.
GermOnline	ENSMUSG00000029102. Mus musculus.
Family and domain databases
Gene3D	2.10.10.10. 1 hit. 2.40.20.10. 1 hit.
InterPro	IPR014394. Coagulation_fac_XIIa/HGFA. IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR000083. Fibronectin_type1. IPR000562. FN_type2_col-bd. IPR000001. Kringle. IPR013806. Kringle-like. IPR018056. Kringle_CS. IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00008. EGF. 2 hits. PF00039. fn1. 1 hit. PF00040. fn2. 1 hit. PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PIRSF	PIRSF001146. Factor_XII_HGFA. 1 hit.
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00181. EGF. 2 hits. SM00059. FN2. 1 hit. SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF57440. Kringle-like. 2 hits. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS00022. EGF_1. 2 hits. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 2 hits. PS01253. FN1_1. 1 hit. PS51091. FN1_2. 1 hit. PS00023. FN2_1. 1 hit. PS51092. FN2_2. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	311300.
SOURCE	Search...

Entry information

Entry name

HGFA_MOUSE

Accession

Primary (citable) accession number: Q9R098
Secondary accession number(s): Q9JKV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents