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Q9R098 (HGFA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hepatocyte growth factor activator

Short name=HGF activator
Short name=HGFA
EC=3.4.21.-
Gene names
Name:Hgfac
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form By similarity.

Subunit structure

Heterodimer of a short chain and a long chain linked by a disulfide bond By similarity.

Subcellular location

Secreted By similarity. Note: Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form By similarity.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 2 EGF-like domains.

Contains 1 fibronectin type-I domain.

Contains 1 fibronectin type-II domain.

Contains 1 kringle domain.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 By similarity
Propeptide35 – 369335Removed in mature form By similarity
PRO_0000027914
Chain370 – 40536Hepatocyte growth factor activator short chain
PRO_0000027915
Chain406 – 653248Hepatocyte growth factor activator long chain
PRO_0000027916

Regions

Domain100 – 14748Fibronectin type-II
Domain157 – 19539EGF-like 1
Domain197 – 23741Fibronectin type-I
Domain238 – 27639EGF-like 2
Domain283 – 36482Kringle
Domain406 – 644239Peptidase S1

Sites

Active site4451Charge relay system By similarity
Active site4951Charge relay system By similarity
Active site5961Charge relay system By similarity

Amino acid modifications

Glycosylation391N-linked (GlcNAc...) Potential
Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation2871N-linked (GlcNAc...) Ref.4
Glycosylation4661N-linked (GlcNAc...) Ref.3
Glycosylation5441N-linked (GlcNAc...) Potential
Disulfide bond105 ↔ 130 By similarity
Disulfide bond119 ↔ 145 By similarity
Disulfide bond161 ↔ 172 By similarity
Disulfide bond166 ↔ 183 By similarity
Disulfide bond185 ↔ 194 By similarity
Disulfide bond199 ↔ 227 By similarity
Disulfide bond225 ↔ 234 By similarity
Disulfide bond242 ↔ 253 By similarity
Disulfide bond247 ↔ 264 By similarity
Disulfide bond266 ↔ 275 By similarity
Disulfide bond283 ↔ 364 By similarity
Disulfide bond304 ↔ 346 By similarity
Disulfide bond335 ↔ 359 By similarity
Disulfide bond392 ↔ 519Interchain (between short and long chains) By similarity
Disulfide bond430 ↔ 446 By similarity
Disulfide bond438 ↔ 508 By similarity
Disulfide bond533 ↔ 602 By similarity
Disulfide bond565 ↔ 581 By similarity
Disulfide bond592 ↔ 620 By similarity

Experimental info

Sequence conflict1641G → W in AAF34712. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9R098 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 88B4B20255DF7FDC

FASTA65370,568
        10         20         30         40         50         60 
MGRQAWISSL CPLPRPCPFL LLLLLLVVPR GAQPQAGRNH TEPPGPNVTA TPVTPTIPVI 

        70         80         90        100        110        120 
SGNVSTSTES APAAETEGPQ SERYPPPSSS SPPGGQVLTE SGQPCRFPFR YGGRMLHSCT 

       130        140        150        160        170        180 
SEGSAYRKWC ATTHNYDRDR AWGYCAEVTL PVEGPAILDP CASGPCLNGG TCSSTHDHGS 

       190        200        210        220        230        240 
YHCSCPLAFT GKDCGTEKCF DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHT 

       250        260        270        280        290        300 
ACLSSPCLNG GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAAS 

       310        320        330        340        350        360 
GLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK DNALSWEYCR 

       370        380        390        400        410        420 
LTACESLARV HSQTPEILAA LPESAPAVRP TCGKRHKKRT FLRPRIIGGS SSLPGSHPWL 

       430        440        450        460        470        480 
AAIYIGNSFC AGSLVHTCWV VSAAHCFANS PPRDSITVVL GQHFFNRTTD VTQTFGIEKY 

       490        500        510        520        530        540 
VPYTLYSVFN PNNHDLVLIR LKKKGERCAV RSQFVQPICL PEAGSSFPTG HKCQIAGWGH 

       550        560        570        580        590        600 
MDENVSSYSN SLLEALVPLV ADHKCSSPEV YGADISPNML CAGYFDCKSD ACQGDSGGPL 

       610        620        630        640        650 
VCEKNGVAYL YGIISWGDGC GRLNKPGVYT RVANYVDWIN DRIRPPKRPV ATS 

« Hide

References

« Hide 'large scale' references
[1]"Mouse hepatocyte growth factor activator gene: its expression not only in the liver but also in the gastrointestinal tract."
Itoh H., Hamasuna R., Kataoka H., Yamauchi M., Miyazawa K., Kitamura N., Koono M.
Biochim. Biophys. Acta 1491:295-302(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Activation of hepatocyte growth factor (HGF) by endogenous HGF activator is required for metanephric kidney morphogenesis in vitro."
van Adelsberg J.S., Sehgal S., Kukes A., Brady C., Barasch J., Yang J., Huan Y.
J. Biol. Chem. 276:15099-15106(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-466.
Strain: C57BL/6.
Tissue: Plasma.
[4]"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
Bernhard O.K., Kapp E.A., Simpson R.J.
J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-287.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF099017 mRNA. Translation: AAF02489.1.
AF224724 mRNA. Translation: AAF34712.1.
RefSeqNP_062320.2. NM_019447.2.
UniGeneMm.27369.

3D structure databases

ProteinModelPortalQ9R098.
SMRQ9R098. Positions 91-646.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.228.

PTM databases

PhosphoSiteQ9R098.

Proteomic databases

PaxDbQ9R098.
PRIDEQ9R098.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030985; ENSMUSP00000030985; ENSMUSG00000029102.
GeneID54426.
KEGGmmu:54426.
UCSCuc008xdj.2. mouse.

Organism-specific databases

CTD3083.
MGIMGI:1859281. Hgfac.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00740000115235.
HOGENOMHOG000237314.
HOVERGENHBG004345.
InParanoidQ9R098.
KOK09631.
OMAGTRHTAC.
OrthoDBEOG75B84T.
PhylomeDBQ9R098.
TreeFamTF329901.

Gene expression databases

ArrayExpressQ9R098.
BgeeQ9R098.
CleanExMM_HGFAC.
GenevestigatorQ9R098.

Family and domain databases

Gene3D2.10.10.10. 1 hit.
2.40.20.10. 1 hit.
InterProIPR014394. Coagulation_fac_XIIa/HGFA.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000083. Fibronectin_type1.
IPR000562. FN_type2_col-bd.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00008. EGF. 2 hits.
PF00039. fn1. 1 hit.
PF00040. fn2. 1 hit.
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFPIRSF001146. Factor_XII_HGFA. 1 hit.
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00181. EGF. 2 hits.
SM00059. FN2. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 2 hits.
PROSITEPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01253. FN1_1. 1 hit.
PS51091. FN1_2. 1 hit.
PS00023. FN2_1. 1 hit.
PS51092. FN2_2. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311300.
PROQ9R098.
SOURCESearch...

Entry information

Entry nameHGFA_MOUSE
AccessionPrimary (citable) accession number: Q9R098
Secondary accession number(s): Q9JKV4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot