SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9R098

- HGFA_MOUSE

UniProt

Q9R098 - HGFA_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Hepatocyte growth factor activator
Gene
Hgfac
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei445 – 4451Charge relay system By similarity
Active sitei495 – 4951Charge relay system By similarity
Active sitei596 – 5961Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.228.

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte growth factor activator (EC:3.4.21.-)
Short name:
HGF activator
Short name:
HGFA
Cleaved into the following 2 chains:
Gene namesi
Name:Hgfac
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1859281. Hgfac.

Subcellular locationi

Secreted By similarity
Note: Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form By similarity.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular space Source: InterPro
  3. rough endoplasmic reticulum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434 By similarity
Add
BLAST
Propeptidei35 – 369335Removed in mature form By similarity
PRO_0000027914Add
BLAST
Chaini370 – 40536Hepatocyte growth factor activator short chain
PRO_0000027915Add
BLAST
Chaini406 – 653248Hepatocyte growth factor activator long chain
PRO_0000027916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi47 – 471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi63 – 631N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi105 ↔ 130 By similarity
Disulfide bondi119 ↔ 145 By similarity
Disulfide bondi161 ↔ 172 By similarity
Disulfide bondi166 ↔ 183 By similarity
Disulfide bondi185 ↔ 194 By similarity
Disulfide bondi199 ↔ 227 By similarity
Disulfide bondi225 ↔ 234 By similarity
Disulfide bondi242 ↔ 253 By similarity
Disulfide bondi247 ↔ 264 By similarity
Disulfide bondi266 ↔ 275 By similarity
Disulfide bondi283 ↔ 364 By similarity
Glycosylationi287 – 2871N-linked (GlcNAc...)1 Publication
Disulfide bondi304 ↔ 346 By similarity
Disulfide bondi335 ↔ 359 By similarity
Disulfide bondi392 ↔ 519Interchain (between short and long chains) By similarity
Disulfide bondi430 ↔ 446 By similarity
Disulfide bondi438 ↔ 508 By similarity
Glycosylationi466 – 4661N-linked (GlcNAc...)1 Publication
Disulfide bondi533 ↔ 602 By similarity
Glycosylationi544 – 5441N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi565 ↔ 581 By similarity
Disulfide bondi592 ↔ 620 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9R098.
PRIDEiQ9R098.

PTM databases

PhosphoSiteiQ9R098.

Expressioni

Gene expression databases

ArrayExpressiQ9R098.
BgeeiQ9R098.
CleanExiMM_HGFAC.
GenevestigatoriQ9R098.

Interactioni

Subunit structurei

Heterodimer of a short chain and a long chain linked by a disulfide bond By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9R098.
SMRiQ9R098. Positions 101-644.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 14748Fibronectin type-II
Add
BLAST
Domaini157 – 19539EGF-like 1
Add
BLAST
Domaini197 – 23741Fibronectin type-I
Add
BLAST
Domaini238 – 27639EGF-like 2
Add
BLAST
Domaini283 – 36482Kringle
Add
BLAST
Domaini406 – 644239Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 kringle domain.

Keywords - Domaini

EGF-like domain, Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00740000115235.
HOGENOMiHOG000237314.
HOVERGENiHBG004345.
InParanoidiQ9R098.
KOiK09631.
OMAiGTRHTAC.
OrthoDBiEOG75B84T.
PhylomeDBiQ9R098.
TreeFamiTF329901.

Family and domain databases

Gene3Di2.10.10.10. 1 hit.
2.40.20.10. 1 hit.
InterProiIPR014394. Coagulation_fac_XIIa/HGFA.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000083. Fibronectin_type1.
IPR000562. FN_type2_col-bd.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00039. fn1. 1 hit.
PF00040. fn2. 1 hit.
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001146. Factor_XII_HGFA. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00181. EGF. 2 hits.
SM00059. FN2. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 2 hits.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01253. FN1_1. 1 hit.
PS51091. FN1_2. 1 hit.
PS00023. FN2_1. 1 hit.
PS51092. FN2_2. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R098-1 [UniParc]FASTAAdd to Basket

« Hide

MGRQAWISSL CPLPRPCPFL LLLLLLVVPR GAQPQAGRNH TEPPGPNVTA    50
TPVTPTIPVI SGNVSTSTES APAAETEGPQ SERYPPPSSS SPPGGQVLTE 100
SGQPCRFPFR YGGRMLHSCT SEGSAYRKWC ATTHNYDRDR AWGYCAEVTL 150
PVEGPAILDP CASGPCLNGG TCSSTHDHGS YHCSCPLAFT GKDCGTEKCF 200
DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHT ACLSSPCLNG 250
GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAAS 300
GLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK 350
DNALSWEYCR LTACESLARV HSQTPEILAA LPESAPAVRP TCGKRHKKRT 400
FLRPRIIGGS SSLPGSHPWL AAIYIGNSFC AGSLVHTCWV VSAAHCFANS 450
PPRDSITVVL GQHFFNRTTD VTQTFGIEKY VPYTLYSVFN PNNHDLVLIR 500
LKKKGERCAV RSQFVQPICL PEAGSSFPTG HKCQIAGWGH MDENVSSYSN 550
SLLEALVPLV ADHKCSSPEV YGADISPNML CAGYFDCKSD ACQGDSGGPL 600
VCEKNGVAYL YGIISWGDGC GRLNKPGVYT RVANYVDWIN DRIRPPKRPV 650
ATS 653
Length:653
Mass (Da):70,568
Last modified:May 1, 2000 - v1
Checksum:i88B4B20255DF7FDC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641G → W in AAF34712. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099017 mRNA. Translation: AAF02489.1.
AF224724 mRNA. Translation: AAF34712.1.
CCDSiCCDS19223.1.
RefSeqiNP_062320.2. NM_019447.2.
UniGeneiMm.27369.

Genome annotation databases

EnsembliENSMUST00000030985; ENSMUSP00000030985; ENSMUSG00000029102.
GeneIDi54426.
KEGGimmu:54426.
UCSCiuc008xdj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099017 mRNA. Translation: AAF02489.1 .
AF224724 mRNA. Translation: AAF34712.1 .
CCDSi CCDS19223.1.
RefSeqi NP_062320.2. NM_019447.2.
UniGenei Mm.27369.

3D structure databases

ProteinModelPortali Q9R098.
SMRi Q9R098. Positions 101-644.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.228.

PTM databases

PhosphoSitei Q9R098.

Proteomic databases

PaxDbi Q9R098.
PRIDEi Q9R098.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030985 ; ENSMUSP00000030985 ; ENSMUSG00000029102 .
GeneIDi 54426.
KEGGi mmu:54426.
UCSCi uc008xdj.2. mouse.

Organism-specific databases

CTDi 3083.
MGIi MGI:1859281. Hgfac.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00740000115235.
HOGENOMi HOG000237314.
HOVERGENi HBG004345.
InParanoidi Q9R098.
KOi K09631.
OMAi GTRHTAC.
OrthoDBi EOG75B84T.
PhylomeDBi Q9R098.
TreeFami TF329901.

Miscellaneous databases

NextBioi 311300.
PROi Q9R098.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9R098.
Bgeei Q9R098.
CleanExi MM_HGFAC.
Genevestigatori Q9R098.

Family and domain databases

Gene3Di 2.10.10.10. 1 hit.
2.40.20.10. 1 hit.
InterProi IPR014394. Coagulation_fac_XIIa/HGFA.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000083. Fibronectin_type1.
IPR000562. FN_type2_col-bd.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00008. EGF. 2 hits.
PF00039. fn1. 1 hit.
PF00040. fn2. 1 hit.
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001146. Factor_XII_HGFA. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00181. EGF. 2 hits.
SM00059. FN2. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 2 hits.
PROSITEi PS00022. EGF_1. 2 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01253. FN1_1. 1 hit.
PS51091. FN1_2. 1 hit.
PS00023. FN2_1. 1 hit.
PS51092. FN2_2. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse hepatocyte growth factor activator gene: its expression not only in the liver but also in the gastrointestinal tract."
    Itoh H., Hamasuna R., Kataoka H., Yamauchi M., Miyazawa K., Kitamura N., Koono M.
    Biochim. Biophys. Acta 1491:295-302(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Activation of hepatocyte growth factor (HGF) by endogenous HGF activator is required for metanephric kidney morphogenesis in vitro."
    van Adelsberg J.S., Sehgal S., Kukes A., Brady C., Barasch J., Yang J., Huan Y.
    J. Biol. Chem. 276:15099-15106(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-466.
    Strain: C57BL/6.
    Tissue: Plasma.
  4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-287.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiHGFA_MOUSE
AccessioniPrimary (citable) accession number: Q9R098
Secondary accession number(s): Q9JKV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi