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Q9R098

- HGFA_MOUSE

UniProt

Q9R098 - HGFA_MOUSE

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Protein

Hepatocyte growth factor activator

Gene

Hgfac

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei445 – 4451Charge relay systemBy similarity
Active sitei495 – 4951Charge relay systemBy similarity
Active sitei596 – 5961Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.228.

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte growth factor activator (EC:3.4.21.-)
Short name:
HGF activator
Short name:
HGFA
Cleaved into the following 2 chains:
Gene namesi
Name:Hgfac
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1859281. Hgfac.

Subcellular locationi

Secreted By similarity
Note: Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular space Source: InterPro
  3. rough endoplasmic reticulum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434By similarityAdd
BLAST
Propeptidei35 – 369335Removed in mature formBy similarityPRO_0000027914Add
BLAST
Chaini370 – 40536Hepatocyte growth factor activator short chainPRO_0000027915Add
BLAST
Chaini406 – 653248Hepatocyte growth factor activator long chainPRO_0000027916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi105 ↔ 130By similarity
Disulfide bondi119 ↔ 145By similarity
Disulfide bondi161 ↔ 172By similarity
Disulfide bondi166 ↔ 183By similarity
Disulfide bondi185 ↔ 194By similarity
Disulfide bondi199 ↔ 227By similarity
Disulfide bondi225 ↔ 234By similarity
Disulfide bondi242 ↔ 253By similarity
Disulfide bondi247 ↔ 264By similarity
Disulfide bondi266 ↔ 275By similarity
Disulfide bondi283 ↔ 364By similarity
Glycosylationi287 – 2871N-linked (GlcNAc...)1 Publication
Disulfide bondi304 ↔ 346By similarity
Disulfide bondi335 ↔ 359By similarity
Disulfide bondi392 ↔ 519Interchain (between short and long chains)PROSITE-ProRule annotation
Disulfide bondi430 ↔ 446By similarity
Disulfide bondi438 ↔ 508By similarity
Glycosylationi466 – 4661N-linked (GlcNAc...)1 Publication
Disulfide bondi533 ↔ 602By similarity
Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi565 ↔ 581By similarity
Disulfide bondi592 ↔ 620By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9R098.
PaxDbiQ9R098.
PRIDEiQ9R098.

PTM databases

PhosphoSiteiQ9R098.

Expressioni

Gene expression databases

BgeeiQ9R098.
CleanExiMM_HGFAC.
ExpressionAtlasiQ9R098. baseline and differential.
GenevestigatoriQ9R098.

Interactioni

Subunit structurei

Heterodimer of a short chain and a long chain linked by a disulfide bond.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9R098.
SMRiQ9R098. Positions 101-646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 14748Fibronectin type-IIPROSITE-ProRule annotationAdd
BLAST
Domaini157 – 19539EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini197 – 23741Fibronectin type-IPROSITE-ProRule annotationAdd
BLAST
Domaini238 – 27639EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini283 – 36482KringlePROSITE-ProRule annotationAdd
BLAST
Domaini406 – 644239Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 fibronectin type-I domain.PROSITE-ProRule annotation
Contains 1 fibronectin type-II domain.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000119133.
HOGENOMiHOG000237314.
HOVERGENiHBG004345.
InParanoidiQ9R098.
KOiK09631.
OMAiGTRHTAC.
OrthoDBiEOG75B84T.
PhylomeDBiQ9R098.
TreeFamiTF329901.

Family and domain databases

Gene3Di2.10.10.10. 1 hit.
2.40.20.10. 1 hit.
InterProiIPR014394. Coagulation_fac_XIIa/HGFA.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000083. Fibronectin_type1.
IPR000562. FN_type2_col-bd.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00039. fn1. 1 hit.
PF00040. fn2. 1 hit.
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001146. Factor_XII_HGFA. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00181. EGF. 2 hits.
SM00059. FN2. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 2 hits.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01253. FN1_1. 1 hit.
PS51091. FN1_2. 1 hit.
PS00023. FN2_1. 1 hit.
PS51092. FN2_2. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R098-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRQAWISSL CPLPRPCPFL LLLLLLVVPR GAQPQAGRNH TEPPGPNVTA
60 70 80 90 100
TPVTPTIPVI SGNVSTSTES APAAETEGPQ SERYPPPSSS SPPGGQVLTE
110 120 130 140 150
SGQPCRFPFR YGGRMLHSCT SEGSAYRKWC ATTHNYDRDR AWGYCAEVTL
160 170 180 190 200
PVEGPAILDP CASGPCLNGG TCSSTHDHGS YHCSCPLAFT GKDCGTEKCF
210 220 230 240 250
DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHT ACLSSPCLNG
260 270 280 290 300
GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAAS
310 320 330 340 350
GLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK
360 370 380 390 400
DNALSWEYCR LTACESLARV HSQTPEILAA LPESAPAVRP TCGKRHKKRT
410 420 430 440 450
FLRPRIIGGS SSLPGSHPWL AAIYIGNSFC AGSLVHTCWV VSAAHCFANS
460 470 480 490 500
PPRDSITVVL GQHFFNRTTD VTQTFGIEKY VPYTLYSVFN PNNHDLVLIR
510 520 530 540 550
LKKKGERCAV RSQFVQPICL PEAGSSFPTG HKCQIAGWGH MDENVSSYSN
560 570 580 590 600
SLLEALVPLV ADHKCSSPEV YGADISPNML CAGYFDCKSD ACQGDSGGPL
610 620 630 640 650
VCEKNGVAYL YGIISWGDGC GRLNKPGVYT RVANYVDWIN DRIRPPKRPV

ATS
Length:653
Mass (Da):70,568
Last modified:May 1, 2000 - v1
Checksum:i88B4B20255DF7FDC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641G → W in AAF34712. (PubMed:11032833)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099017 mRNA. Translation: AAF02489.1.
AF224724 mRNA. Translation: AAF34712.1.
CCDSiCCDS19223.1.
RefSeqiNP_062320.2. NM_019447.2.
UniGeneiMm.27369.

Genome annotation databases

EnsembliENSMUST00000030985; ENSMUSP00000030985; ENSMUSG00000029102.
GeneIDi54426.
KEGGimmu:54426.
UCSCiuc008xdj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099017 mRNA. Translation: AAF02489.1 .
AF224724 mRNA. Translation: AAF34712.1 .
CCDSi CCDS19223.1.
RefSeqi NP_062320.2. NM_019447.2.
UniGenei Mm.27369.

3D structure databases

ProteinModelPortali Q9R098.
SMRi Q9R098. Positions 101-646.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.228.

PTM databases

PhosphoSitei Q9R098.

Proteomic databases

MaxQBi Q9R098.
PaxDbi Q9R098.
PRIDEi Q9R098.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030985 ; ENSMUSP00000030985 ; ENSMUSG00000029102 .
GeneIDi 54426.
KEGGi mmu:54426.
UCSCi uc008xdj.2. mouse.

Organism-specific databases

CTDi 3083.
MGIi MGI:1859281. Hgfac.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00760000119133.
HOGENOMi HOG000237314.
HOVERGENi HBG004345.
InParanoidi Q9R098.
KOi K09631.
OMAi GTRHTAC.
OrthoDBi EOG75B84T.
PhylomeDBi Q9R098.
TreeFami TF329901.

Miscellaneous databases

NextBioi 311300.
PROi Q9R098.
SOURCEi Search...

Gene expression databases

Bgeei Q9R098.
CleanExi MM_HGFAC.
ExpressionAtlasi Q9R098. baseline and differential.
Genevestigatori Q9R098.

Family and domain databases

Gene3Di 2.10.10.10. 1 hit.
2.40.20.10. 1 hit.
InterProi IPR014394. Coagulation_fac_XIIa/HGFA.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000083. Fibronectin_type1.
IPR000562. FN_type2_col-bd.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00008. EGF. 2 hits.
PF00039. fn1. 1 hit.
PF00040. fn2. 1 hit.
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001146. Factor_XII_HGFA. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00181. EGF. 2 hits.
SM00059. FN2. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 2 hits.
PROSITEi PS00022. EGF_1. 2 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01253. FN1_1. 1 hit.
PS51091. FN1_2. 1 hit.
PS00023. FN2_1. 1 hit.
PS51092. FN2_2. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse hepatocyte growth factor activator gene: its expression not only in the liver but also in the gastrointestinal tract."
    Itoh H., Hamasuna R., Kataoka H., Yamauchi M., Miyazawa K., Kitamura N., Koono M.
    Biochim. Biophys. Acta 1491:295-302(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Activation of hepatocyte growth factor (HGF) by endogenous HGF activator is required for metanephric kidney morphogenesis in vitro."
    van Adelsberg J.S., Sehgal S., Kukes A., Brady C., Barasch J., Yang J., Huan Y.
    J. Biol. Chem. 276:15099-15106(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-466.
    Strain: C57BL/6.
    Tissue: Plasma.
  4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-287.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiHGFA_MOUSE
AccessioniPrimary (citable) accession number: Q9R098
Secondary accession number(s): Q9JKV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3