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Protein

Kunitz-type protease inhibitor 1

Gene

Spint1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of HGF activator.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei254 – 255Reactive bondBy similarity2
Sitei379 – 380Reactive bondBy similarity2

GO - Molecular functioni

GO - Biological processi

  • branching involved in labyrinthine layer morphogenesis Source: MGI
  • cellular response to BMP stimulus Source: Ensembl
  • embryonic placenta development Source: MGI
  • extracellular matrix organization Source: MGI
  • negative regulation of neural precursor cell proliferation Source: Ensembl
  • neural tube closure Source: MGI
  • placenta blood vessel development Source: MGI
  • positive regulation of glial cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-MMU-6806942. MET Receptor Activation.

Protein family/group databases

MEROPSiI02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type protease inhibitor 1
Alternative name(s):
Hepatocyte growth factor activator inhibitor type 1
Short name:
HAI-1
Gene namesi
Name:Spint1
Synonyms:Hai1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1338033. Spint1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001688430 – 507Kunitz-type protease inhibitor 1Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi229N-linked (GlcNAc...)1 Publication1
Disulfide bondi244 ↔ 294By similarity
Disulfide bondi253 ↔ 277By similarity
Disulfide bondi269 ↔ 290By similarity
Disulfide bondi320 ↔ 338By similarity
Disulfide bondi332 ↔ 347By similarity
Disulfide bondi369 ↔ 419By similarity
Disulfide bondi378 ↔ 402By similarity
Disulfide bondi394 ↔ 415By similarity
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9R097.
PaxDbiQ9R097.
PeptideAtlasiQ9R097.
PRIDEiQ9R097.

PTM databases

PhosphoSitePlusiQ9R097.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027315.
CleanExiMM_SPINT1.
GenevisibleiQ9R097. MM.

Interactioni

Subunit structurei

Interacts with HGFAC.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028783.

Structurei

3D structure databases

ProteinModelPortaliQ9R097.
SMRiQ9R097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 134MANSCPROSITE-ProRule annotationAdd BLAST84
Domaini244 – 294BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST51
Domaini312 – 348LDL-receptor class APROSITE-ProRule annotationAdd BLAST37
Domaini369 – 419BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51

Domaini

This inhibitor contains two inhibitory domains.

Sequence similaritiesi

Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 1 MANSC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00860000133796.
HOGENOMiHOG000128104.
HOVERGENiHBG061664.
InParanoidiQ9R097.
KOiK15619.
OMAiECKLACR.
OrthoDBiEOG091G0612.
TreeFamiTF325867.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR013980. MANSC_dom.
IPR011106. MANSC_N.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF07502. MANEC. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
SM00192. LDLa. 1 hit.
SM00765. MANEC. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50986. MANSC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRRLARAS ISAVGVWLLC ALGLQATEAE LPSAPAELPG GAACLSRFTS
60 70 80 90 100
GVPSFVLDTE ASVSNGATFL GSPTARRGWD CVRSCCTTQN CNLALVELQP
110 120 130 140 150
DGGEDAISAC FLMNCLYEQN FVCKFAPKEG FINYLTQELY RSYRELRTRG
160 170 180 190 200
FGGSRIPRIW MGIDLKVQLQ KPLVLNEADN TDWHLLQGDS DVRVERKRPE
210 220 230 240 250
EVELWGLKEG TYLFQLTRTD SDQPEETANL TITVLTAKQT EDYCLASYKV
260 270 280 290 300
GRCRGSFPRW YYDPKEQICK SFTFGGCLGN KNNYLREEEC MLACKDVQGI
310 320 330 340 350
SPKRHHPVCS GSCHATQFRC SNGCCIDGFL ECDDTPDCPD GSDEATCEKY
360 370 380 390 400
TSGFDELQNI HFLSDKGYCA ELPDTGFCKE NIPRWYYNPF SERCARFTYG
410 420 430 440 450
GCYGNKNNFE EEQQCLESCR GISKKDVFGL RREGSIPTVG SAEVAIAVFL
460 470 480 490 500
VICIIVVLTI LGYCFFKNQR KEFHSPLHHP PPTPASSTVS TTEDTEHLVY

NHTTQPL
Length:507
Mass (Da):56,591
Last modified:July 27, 2011 - v2
Checksum:iF7F9CCF2693D1F8D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102G → R in AAF02490 (PubMed:11406276).Curated1
Sequence conflicti238K → M in AAF02490 (PubMed:11406276).Curated1
Sequence conflicti325C → S in AAF02490 (PubMed:11406276).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099018 mRNA. Translation: AAF02490.1.
AL929318 Genomic DNA. Translation: CAM25337.1.
CH466519 Genomic DNA. Translation: EDL27946.1.
BC005769 mRNA. Translation: AAH05769.1.
CCDSiCCDS16597.1.
RefSeqiNP_058603.2. NM_016907.3.
XP_006499125.1. XM_006499062.3.
UniGeneiMm.104955.

Genome annotation databases

EnsembliENSMUST00000028783; ENSMUSP00000028783; ENSMUSG00000027315.
ENSMUST00000110816; ENSMUSP00000106440; ENSMUSG00000027315.
ENSMUST00000110817; ENSMUSP00000106441; ENSMUSG00000027315.
GeneIDi20732.
KEGGimmu:20732.
UCSCiuc008ltk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099018 mRNA. Translation: AAF02490.1.
AL929318 Genomic DNA. Translation: CAM25337.1.
CH466519 Genomic DNA. Translation: EDL27946.1.
BC005769 mRNA. Translation: AAH05769.1.
CCDSiCCDS16597.1.
RefSeqiNP_058603.2. NM_016907.3.
XP_006499125.1. XM_006499062.3.
UniGeneiMm.104955.

3D structure databases

ProteinModelPortaliQ9R097.
SMRiQ9R097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028783.

Protein family/group databases

MEROPSiI02.007.

PTM databases

PhosphoSitePlusiQ9R097.

Proteomic databases

MaxQBiQ9R097.
PaxDbiQ9R097.
PeptideAtlasiQ9R097.
PRIDEiQ9R097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028783; ENSMUSP00000028783; ENSMUSG00000027315.
ENSMUST00000110816; ENSMUSP00000106440; ENSMUSG00000027315.
ENSMUST00000110817; ENSMUSP00000106441; ENSMUSG00000027315.
GeneIDi20732.
KEGGimmu:20732.
UCSCiuc008ltk.1. mouse.

Organism-specific databases

CTDi6692.
MGIiMGI:1338033. Spint1.

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00860000133796.
HOGENOMiHOG000128104.
HOVERGENiHBG061664.
InParanoidiQ9R097.
KOiK15619.
OMAiECKLACR.
OrthoDBiEOG091G0612.
TreeFamiTF325867.

Enzyme and pathway databases

ReactomeiR-MMU-6806942. MET Receptor Activation.

Miscellaneous databases

ChiTaRSiSpint1. mouse.
PROiQ9R097.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027315.
CleanExiMM_SPINT1.
GenevisibleiQ9R097. MM.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR013980. MANSC_dom.
IPR011106. MANSC_N.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF07502. MANEC. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
SM00192. LDLa. 1 hit.
SM00765. MANEC. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50986. MANSC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPIT1_MOUSE
AccessioniPrimary (citable) accession number: Q9R097
Secondary accession number(s): Q99J04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.