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Protein

Kunitz-type protease inhibitor 1

Gene

Spint1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of HGF activator.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei254 – 2552Reactive bondBy similarity
Sitei379 – 3802Reactive bondBy similarity

GO - Molecular functioni

GO - Biological processi

  • branching involved in labyrinthine layer morphogenesis Source: MGI
  • cellular response to BMP stimulus Source: Ensembl
  • embryonic placenta development Source: MGI
  • extracellular matrix organization Source: MGI
  • negative regulation of neural precursor cell proliferation Source: Ensembl
  • neural tube closure Source: MGI
  • placenta blood vessel development Source: MGI
  • positive regulation of glial cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type protease inhibitor 1
Alternative name(s):
Hepatocyte growth factor activator inhibitor type 1
Short name:
HAI-1
Gene namesi
Name:Spint1
Synonyms:Hai1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1338033. Spint1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 507478Kunitz-type protease inhibitor 1PRO_0000016884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi229 – 2291N-linked (GlcNAc...)1 Publication
Disulfide bondi244 ↔ 294By similarity
Disulfide bondi253 ↔ 277By similarity
Disulfide bondi269 ↔ 290By similarity
Disulfide bondi320 ↔ 338By similarity
Disulfide bondi332 ↔ 347By similarity
Disulfide bondi369 ↔ 419By similarity
Disulfide bondi378 ↔ 402By similarity
Disulfide bondi394 ↔ 415By similarity
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9R097.
PaxDbiQ9R097.
PeptideAtlasiQ9R097.
PRIDEiQ9R097.

PTM databases

PhosphoSiteiQ9R097.

Expressioni

Gene expression databases

BgeeiQ9R097.
CleanExiMM_SPINT1.
GenevisibleiQ9R097. MM.

Interactioni

Subunit structurei

Interacts with HGFAC.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028783.

Structurei

3D structure databases

ProteinModelPortaliQ9R097.
SMRiQ9R097. Positions 41-146, 239-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 13484MANSCPROSITE-ProRule annotationAdd
BLAST
Domaini244 – 29451BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd
BLAST
Domaini312 – 34837LDL-receptor class APROSITE-ProRule annotationAdd
BLAST
Domaini369 – 41951BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd
BLAST

Domaini

This inhibitor contains two inhibitory domains.

Sequence similaritiesi

Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 1 MANSC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000128104.
HOVERGENiHBG061664.
InParanoidiQ9R097.
KOiK15619.
OMAiREEECKL.
OrthoDBiEOG754HSR.
TreeFamiTF325867.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR013980. MANSC_dom.
IPR011106. MANSC_N.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF07502. MANEC. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
SM00192. LDLa. 1 hit.
SM00765. MANEC. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50986. MANSC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRRLARAS ISAVGVWLLC ALGLQATEAE LPSAPAELPG GAACLSRFTS
60 70 80 90 100
GVPSFVLDTE ASVSNGATFL GSPTARRGWD CVRSCCTTQN CNLALVELQP
110 120 130 140 150
DGGEDAISAC FLMNCLYEQN FVCKFAPKEG FINYLTQELY RSYRELRTRG
160 170 180 190 200
FGGSRIPRIW MGIDLKVQLQ KPLVLNEADN TDWHLLQGDS DVRVERKRPE
210 220 230 240 250
EVELWGLKEG TYLFQLTRTD SDQPEETANL TITVLTAKQT EDYCLASYKV
260 270 280 290 300
GRCRGSFPRW YYDPKEQICK SFTFGGCLGN KNNYLREEEC MLACKDVQGI
310 320 330 340 350
SPKRHHPVCS GSCHATQFRC SNGCCIDGFL ECDDTPDCPD GSDEATCEKY
360 370 380 390 400
TSGFDELQNI HFLSDKGYCA ELPDTGFCKE NIPRWYYNPF SERCARFTYG
410 420 430 440 450
GCYGNKNNFE EEQQCLESCR GISKKDVFGL RREGSIPTVG SAEVAIAVFL
460 470 480 490 500
VICIIVVLTI LGYCFFKNQR KEFHSPLHHP PPTPASSTVS TTEDTEHLVY

NHTTQPL
Length:507
Mass (Da):56,591
Last modified:July 27, 2011 - v2
Checksum:iF7F9CCF2693D1F8D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021G → R in AAF02490 (PubMed:11406276).Curated
Sequence conflicti238 – 2381K → M in AAF02490 (PubMed:11406276).Curated
Sequence conflicti325 – 3251C → S in AAF02490 (PubMed:11406276).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099018 mRNA. Translation: AAF02490.1.
AL929318 Genomic DNA. Translation: CAM25337.1.
CH466519 Genomic DNA. Translation: EDL27946.1.
BC005769 mRNA. Translation: AAH05769.1.
CCDSiCCDS16597.1.
RefSeqiNP_058603.2. NM_016907.3.
XP_006499125.1. XM_006499062.2.
UniGeneiMm.104955.

Genome annotation databases

EnsembliENSMUST00000028783; ENSMUSP00000028783; ENSMUSG00000027315.
ENSMUST00000110816; ENSMUSP00000106440; ENSMUSG00000027315.
ENSMUST00000110817; ENSMUSP00000106441; ENSMUSG00000027315.
GeneIDi20732.
KEGGimmu:20732.
UCSCiuc008ltk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099018 mRNA. Translation: AAF02490.1.
AL929318 Genomic DNA. Translation: CAM25337.1.
CH466519 Genomic DNA. Translation: EDL27946.1.
BC005769 mRNA. Translation: AAH05769.1.
CCDSiCCDS16597.1.
RefSeqiNP_058603.2. NM_016907.3.
XP_006499125.1. XM_006499062.2.
UniGeneiMm.104955.

3D structure databases

ProteinModelPortaliQ9R097.
SMRiQ9R097. Positions 41-146, 239-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028783.

Protein family/group databases

MEROPSiI02.007.

PTM databases

PhosphoSiteiQ9R097.

Proteomic databases

MaxQBiQ9R097.
PaxDbiQ9R097.
PeptideAtlasiQ9R097.
PRIDEiQ9R097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028783; ENSMUSP00000028783; ENSMUSG00000027315.
ENSMUST00000110816; ENSMUSP00000106440; ENSMUSG00000027315.
ENSMUST00000110817; ENSMUSP00000106441; ENSMUSG00000027315.
GeneIDi20732.
KEGGimmu:20732.
UCSCiuc008ltk.1. mouse.

Organism-specific databases

CTDi6692.
MGIiMGI:1338033. Spint1.

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000128104.
HOVERGENiHBG061664.
InParanoidiQ9R097.
KOiK15619.
OMAiREEECKL.
OrthoDBiEOG754HSR.
TreeFamiTF325867.

Miscellaneous databases

ChiTaRSiSpint1. mouse.
PROiQ9R097.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R097.
CleanExiMM_SPINT1.
GenevisibleiQ9R097. MM.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR013980. MANSC_dom.
IPR011106. MANSC_N.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF07502. MANEC. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
SM00192. LDLa. 1 hit.
SM00765. MANEC. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50986. MANSC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse hepatocyte growth factor activator inhibitor type 1 (HAI-1) and type 2 (HAI-2)/placental bikunin genes and their promoters."
    Itoh H., Kataoka H., Meng J.Y., Hamasuna R., Kitamura N., Koono M.
    Biochim. Biophys. Acta 1519:92-95(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary tumor.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-229.

Entry informationi

Entry nameiSPIT1_MOUSE
AccessioniPrimary (citable) accession number: Q9R097
Secondary accession number(s): Q99J04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.