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Q9R088

- KITM_MOUSE

UniProt

Q9R088 - KITM_MOUSE

Protein

Thymidine kinase 2, mitochondrial

Gene

Tk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (24 Jan 2001)
      Previous versions | rss
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    Functioni

    Deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine. Also phosphorylates anti-viral and anti-cancer nucleoside analogs.

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei86 – 861SubstrateBy similarity
    Binding sitei104 – 1041SubstrateBy similarity
    Binding sitei115 – 1151SubstrateBy similarity
    Active sitei138 – 1381Proton acceptorSequence Analysis
    Binding sitei139 – 1391SubstrateBy similarity
    Binding sitei206 – 2061SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 709ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphotransferase activity, alcohol group as acceptor Source: InterPro
    3. thymidine kinase activity Source: MGI

    GO - Biological processi

    1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
    2. deoxyribonucleotide metabolic process Source: MGI
    3. mitochondrial DNA metabolic process Source: MGI
    4. mitochondrial DNA replication Source: MGI

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.21. 3474.
    SABIO-RKQ9R088.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase 2, mitochondrial (EC:2.7.1.21)
    Alternative name(s):
    Mt-TK
    Gene namesi
    Name:Tk2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1913266. Tk2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: MGI
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3838MitochondrionSequence AnalysisAdd
    BLAST
    Chaini39 – 270232Thymidine kinase 2, mitochondrialPRO_0000016844Add
    BLAST

    Proteomic databases

    PaxDbiQ9R088.
    PRIDEiQ9R088.

    PTM databases

    PhosphoSiteiQ9R088.

    Expressioni

    Tissue specificityi

    Found in most tissues; highly expressed in liver.

    Gene expression databases

    CleanExiMM_TK2.
    GenevestigatoriQ9R088.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R088.
    SMRiQ9R088. Positions 58-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DCK/DGK family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1428.
    HOGENOMiHOG000006872.
    HOVERGENiHBG006216.
    InParanoidiQ9R088.
    PhylomeDBiQ9R088.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000705. DNK. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9R088-1 [UniParc]FASTAAdd to Basket

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    MLLRSLRSWA ARSPRSVGPG SSGSPGSLDS GAGPLWAPRR AWPPDKDREN    50
    DKEKKAVVCI EGNIASGKTT CLEFFSNTTD VEVLMEPVLK WRNVHGHNPL 100
    SLMYHDASRW GLTLQTYVQL TMLDQHTRPQ MSPVRLMERS IYSARYIFVE 150
    NLYRGGKMPE VDYAILSEWF DWIVRNIDVS VDLIVYLRTT PEICYQRLKM 200
    RCREEEKVIP MEYLHAIHRL YEEWLVNGSL FPAAAPVLVI EADHNLEKML 250
    ELFEQNRARI LTPENWKHGP 270
    Length:270
    Mass (Da):31,209
    Last modified:January 24, 2001 - v2
    Checksum:i886F5B80D2C3EFE2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141P → L in AAF08104. (PubMed:10571069)Curated
    Sequence conflicti23 – 231G → R in AAF08104. (PubMed:10571069)Curated
    Sequence conflicti155 – 1551G → S in AAF08104. (PubMed:10571069)Curated
    Sequence conflicti269 – 2702GP → WTLGLSDLQDSARNSPARAR CHGPRA(PubMed:10571069)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF105217 mRNA. Translation: AAF08104.1.
    AJ249341 mRNA. Translation: CAC07190.2.
    CCDSiCCDS22573.1.
    UniGeneiMm.183110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF105217 mRNA. Translation: AAF08104.1 .
    AJ249341 mRNA. Translation: CAC07190.2 .
    CCDSi CCDS22573.1.
    UniGenei Mm.183110.

    3D structure databases

    ProteinModelPortali Q9R088.
    SMRi Q9R088. Positions 58-247.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q9R088.

    PTM databases

    PhosphoSitei Q9R088.

    Proteomic databases

    PaxDbi Q9R088.
    PRIDEi Q9R088.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:1913266. Tk2.

    Phylogenomic databases

    eggNOGi COG1428.
    HOGENOMi HOG000006872.
    HOVERGENi HBG006216.
    InParanoidi Q9R088.
    PhylomeDBi Q9R088.

    Enzyme and pathway databases

    BRENDAi 2.7.1.21. 3474.
    SABIO-RK Q9R088.

    Miscellaneous databases

    PROi Q9R088.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_TK2.
    Genevestigatori Q9R088.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10513. PTHR10513. 1 hit.
    Pfami PF01712. dNK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000705. DNK. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of mouse mitochondrial thymidine kinase 2 cDNA."
      Wettin K., Johansson M., Zheng X., Zhu C., Karlsson A.
      FEBS Lett. 460:103-106(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and characterization of full length mouse thymidine kinase 2: the N -terminal sequence directs import of the precursor protein into mitochondria."
      Wang L., Eriksson S.
      Biochem. J. 351:469-476(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiKITM_MOUSE
    AccessioniPrimary (citable) accession number: Q9R088
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: January 24, 2001
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3