Q9R088 (KITM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase 2, mitochondrial
EC=2.7.1.21

Alternative name(s):
Mt-TK

Gene names

Name:

Tk2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine. Also phosphorylates anti-viral and anti-cancer nucleoside analogs.
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion.
Tissue specificity	Found in most tissues; highly expressed in liver.
Sequence similarities	Belongs to the DCK/DGK family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	deoxyribonucleotide metabolic process Inferred from mutant phenotype PubMed 18467430. Source: MGI mitochondrial DNA replication Inferred from mutant phenotype PubMed 18434326. Source: MGI
Cellular_component	mitochondrial inner membrane Inferred from direct assay PubMed 12865426. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro thymidine kinase activity Inferred from sequence or structural similarity Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 38	38	Mitochondrion Potential
	Chain	39 – 270	232	Thymidine kinase 2, mitochondrial		PRO_0000016844
Regions
	Nucleotide binding	62 – 70	9	ATP By similarity
Sites
	Active site	138	1	Proton acceptor Potential
	Binding site	86	1	Substrate By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	206	1	Substrate By similarity
Experimental info
	Sequence conflict	14	1	P → L in AAF08104. Ref.1
	Sequence conflict	23	1	G → R in AAF08104. Ref.1
	Sequence conflict	155	1	G → S in AAF08104. Ref.1
	Sequence conflict	269 – 270	2	GP → WTLGLSDLQDSARNSPARAR CHGPRA Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9R088 [UniParc]. Last modified January 24, 2001. Version 2. Checksum: 886F5B80D2C3EFE2	FASTA	270	31,209
	10 20 30 40 50 60 MLLRSLRSWA ARSPRSVGPG SSGSPGSLDS GAGPLWAPRR AWPPDKDREN DKEKKAVVCI 70 80 90 100 110 120 EGNIASGKTT CLEFFSNTTD VEVLMEPVLK WRNVHGHNPL SLMYHDASRW GLTLQTYVQL 130 140 150 160 170 180 TMLDQHTRPQ MSPVRLMERS IYSARYIFVE NLYRGGKMPE VDYAILSEWF DWIVRNIDVS 190 200 210 220 230 240 VDLIVYLRTT PEICYQRLKM RCREEEKVIP MEYLHAIHRL YEEWLVNGSL FPAAAPVLVI 250 260 270 EADHNLEKML ELFEQNRARI LTPENWKHGP

References

[1]	"Cloning of mouse mitochondrial thymidine kinase 2 cDNA." Wettin K., Johansson M., Zheng X., Zhu C., Karlsson A. FEBS Lett. 460:103-106(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of full length mouse thymidine kinase 2: the N -terminal sequence directs import of the precursor protein into mitochondria." Wang L., Eriksson S. Biochem. J. 351:469-476(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF105217 mRNA. Translation: AAF08104.1. AJ249341 mRNA. Translation: CAC07190.2.
IPI	IPI00126992.
UniGene	Mm.183110.
3D structure databases
ProteinModelPortal	Q9R088.
SMR	Q9R088. Positions 58-255.
ModBase	Search...
PTM databases
PhosphoSite	Q9R088.
Proteomic databases
PaxDb	Q9R088.
PRIDE	Q9R088.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:1913266. Tk2.
Phylogenomic databases
eggNOG	COG1428.
HOGENOM	HOG000006872.
HOVERGEN	HBG006216.
InParanoid	Q9R088.
OrthoDB	EOG4B8JF5.
Enzyme and pathway databases
BRENDA	2.7.1.21. 3474.
SABIO-RK	Q9R088.
Gene expression databases
CleanEx	MM_TK2.
Genevestigator	Q9R088.
GermOnline	ENSMUSG00000035824. Mus musculus.
Family and domain databases
InterPro	IPR002624. Deoxynucleoside_kinase. [Graphical view]
PANTHER	PTHR10513. PTHR10513. 1 hit.
Pfam	PF01712. dNK. 1 hit. [Graphical view]
PIRSF	PIRSF000705. DNK. 1 hit.
ProtoNet	Search...
Other
BindingDB	Q9R088.
ChEMBL	CHEMBL3714.
SOURCE	Search...

Entry information

Entry name

KITM_MOUSE

Accession

Primary (citable) accession number: Q9R088

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 24, 2001
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program