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Q9R088 (KITM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase 2, mitochondrial

EC=2.7.1.21
Alternative name(s):
Mt-TK
Gene names
Name:Tk2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine. Also phosphorylates anti-viral and anti-cancer nucleoside analogs.

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion.

Tissue specificity

Found in most tissues; highly expressed in liver.

Sequence similarities

Belongs to the DCK/DGK family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3838Mitochondrion Potential
Chain39 – 270232Thymidine kinase 2, mitochondrial
PRO_0000016844

Regions

Nucleotide binding62 – 709ATP By similarity

Sites

Active site1381Proton acceptor Potential
Binding site861Substrate By similarity
Binding site1041Substrate By similarity
Binding site1151Substrate By similarity
Binding site1391Substrate By similarity
Binding site2061Substrate By similarity

Experimental info

Sequence conflict141P → L in AAF08104. Ref.1
Sequence conflict231G → R in AAF08104. Ref.1
Sequence conflict1551G → S in AAF08104. Ref.1
Sequence conflict269 – 2702GP → WTLGLSDLQDSARNSPARAR CHGPRA Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9R088 [UniParc].

Last modified January 24, 2001. Version 2.
Checksum: 886F5B80D2C3EFE2

FASTA27031,209
        10         20         30         40         50         60 
MLLRSLRSWA ARSPRSVGPG SSGSPGSLDS GAGPLWAPRR AWPPDKDREN DKEKKAVVCI 

        70         80         90        100        110        120 
EGNIASGKTT CLEFFSNTTD VEVLMEPVLK WRNVHGHNPL SLMYHDASRW GLTLQTYVQL 

       130        140        150        160        170        180 
TMLDQHTRPQ MSPVRLMERS IYSARYIFVE NLYRGGKMPE VDYAILSEWF DWIVRNIDVS 

       190        200        210        220        230        240 
VDLIVYLRTT PEICYQRLKM RCREEEKVIP MEYLHAIHRL YEEWLVNGSL FPAAAPVLVI 

       250        260        270 
EADHNLEKML ELFEQNRARI LTPENWKHGP 

« Hide

References

[1]"Cloning of mouse mitochondrial thymidine kinase 2 cDNA."
Wettin K., Johansson M., Zheng X., Zhu C., Karlsson A.
FEBS Lett. 460:103-106(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and characterization of full length mouse thymidine kinase 2: the N -terminal sequence directs import of the precursor protein into mitochondria."
Wang L., Eriksson S.
Biochem. J. 351:469-476(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF105217 mRNA. Translation: AAF08104.1.
AJ249341 mRNA. Translation: CAC07190.2.
UniGeneMm.183110.

3D structure databases

ProteinModelPortalQ9R088.
SMRQ9R088. Positions 58-247.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ9R088.

PTM databases

PhosphoSiteQ9R088.

Proteomic databases

PaxDbQ9R088.
PRIDEQ9R088.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:1913266. Tk2.

Phylogenomic databases

eggNOGCOG1428.
HOGENOMHOG000006872.
HOVERGENHBG006216.
InParanoidQ9R088.
PhylomeDBQ9R088.

Enzyme and pathway databases

BRENDA2.7.1.21. 3474.
SABIO-RKQ9R088.

Gene expression databases

CleanExMM_TK2.
GenevestigatorQ9R088.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR002624. Deoxynucleoside_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10513. PTHR10513. 1 hit.
PfamPF01712. dNK. 1 hit.
[Graphical view]
PIRSFPIRSF000705. DNK. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

PROQ9R088.
SOURCESearch...

Entry information

Entry nameKITM_MOUSE
AccessionPrimary (citable) accession number: Q9R088
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot