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Q9R088

- KITM_MOUSE

UniProt

Q9R088 - KITM_MOUSE

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Protein

Thymidine kinase 2, mitochondrial

Gene

Tk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine. Also phosphorylates anti-viral and anti-cancer nucleoside analogs.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861SubstrateBy similarity
Binding sitei104 – 1041SubstrateBy similarity
Binding sitei115 – 1151SubstrateBy similarity
Active sitei138 – 1381Proton acceptorSequence Analysis
Binding sitei139 – 1391SubstrateBy similarity
Binding sitei206 – 2061SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 709ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: MGI

GO - Biological processi

  1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
  2. deoxyribonucleotide metabolic process Source: MGI
  3. mitochondrial DNA metabolic process Source: MGI
  4. mitochondrial DNA replication Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.21. 3474.
SABIO-RKQ9R088.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase 2, mitochondrial (EC:2.7.1.21)
Alternative name(s):
Mt-TK
Gene namesi
Name:Tk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1913266. Tk2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: MGI
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838MitochondrionSequence AnalysisAdd
BLAST
Chaini39 – 270232Thymidine kinase 2, mitochondrialPRO_0000016844Add
BLAST

Proteomic databases

MaxQBiQ9R088.
PaxDbiQ9R088.
PRIDEiQ9R088.

PTM databases

PhosphoSiteiQ9R088.

Expressioni

Tissue specificityi

Found in most tissues; highly expressed in liver.

Gene expression databases

CleanExiMM_TK2.
GenevestigatoriQ9R088.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliQ9R088.
SMRiQ9R088. Positions 58-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1428.
HOGENOMiHOG000006872.
HOVERGENiHBG006216.
InParanoidiQ9R088.
PhylomeDBiQ9R088.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R088-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLRSLRSWA ARSPRSVGPG SSGSPGSLDS GAGPLWAPRR AWPPDKDREN
60 70 80 90 100
DKEKKAVVCI EGNIASGKTT CLEFFSNTTD VEVLMEPVLK WRNVHGHNPL
110 120 130 140 150
SLMYHDASRW GLTLQTYVQL TMLDQHTRPQ MSPVRLMERS IYSARYIFVE
160 170 180 190 200
NLYRGGKMPE VDYAILSEWF DWIVRNIDVS VDLIVYLRTT PEICYQRLKM
210 220 230 240 250
RCREEEKVIP MEYLHAIHRL YEEWLVNGSL FPAAAPVLVI EADHNLEKML
260 270
ELFEQNRARI LTPENWKHGP
Length:270
Mass (Da):31,209
Last modified:January 24, 2001 - v2
Checksum:i886F5B80D2C3EFE2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141P → L in AAF08104. (PubMed:10571069)Curated
Sequence conflicti23 – 231G → R in AAF08104. (PubMed:10571069)Curated
Sequence conflicti155 – 1551G → S in AAF08104. (PubMed:10571069)Curated
Sequence conflicti269 – 2702GP → WTLGLSDLQDSARNSPARAR CHGPRA(PubMed:10571069)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105217 mRNA. Translation: AAF08104.1.
AJ249341 mRNA. Translation: CAC07190.2.
CCDSiCCDS22573.1.
UniGeneiMm.183110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105217 mRNA. Translation: AAF08104.1 .
AJ249341 mRNA. Translation: CAC07190.2 .
CCDSi CCDS22573.1.
UniGenei Mm.183110.

3D structure databases

ProteinModelPortali Q9R088.
SMRi Q9R088. Positions 58-247.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9R088.

Proteomic databases

MaxQBi Q9R088.
PaxDbi Q9R088.
PRIDEi Q9R088.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:1913266. Tk2.

Phylogenomic databases

eggNOGi COG1428.
HOGENOMi HOG000006872.
HOVERGENi HBG006216.
InParanoidi Q9R088.
PhylomeDBi Q9R088.

Enzyme and pathway databases

BRENDAi 2.7.1.21. 3474.
SABIO-RK Q9R088.

Miscellaneous databases

PROi Q9R088.
SOURCEi Search...

Gene expression databases

CleanExi MM_TK2.
Genevestigatori Q9R088.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10513. PTHR10513. 1 hit.
Pfami PF01712. dNK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000705. DNK. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of mouse mitochondrial thymidine kinase 2 cDNA."
    Wettin K., Johansson M., Zheng X., Zhu C., Karlsson A.
    FEBS Lett. 460:103-106(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of full length mouse thymidine kinase 2: the N -terminal sequence directs import of the precursor protein into mitochondria."
    Wang L., Eriksson S.
    Biochem. J. 351:469-476(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiKITM_MOUSE
AccessioniPrimary (citable) accession number: Q9R088
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3