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Q9R088

- KITM_MOUSE

UniProt

Q9R088 - KITM_MOUSE

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Protein
Thymidine kinase 2, mitochondrial
Gene
Tk2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine. Also phosphorylates anti-viral and anti-cancer nucleoside analogs.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861Substrate By similarity
Binding sitei104 – 1041Substrate By similarity
Binding sitei115 – 1151Substrate By similarity
Active sitei138 – 1381Proton acceptor Reviewed prediction
Binding sitei139 – 1391Substrate By similarity
Binding sitei206 – 2061Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 709ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphotransferase activity, alcohol group as acceptor Source: InterPro
  3. thymidine kinase activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
  2. deoxyribonucleotide metabolic process Source: MGI
  3. mitochondrial DNA metabolic process Source: MGI
  4. mitochondrial DNA replication Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.21. 3474.
SABIO-RKQ9R088.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase 2, mitochondrial (EC:2.7.1.21)
Alternative name(s):
Mt-TK
Gene namesi
Name:Tk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1913266. Tk2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: MGI
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838Mitochondrion Reviewed prediction
Add
BLAST
Chaini39 – 270232Thymidine kinase 2, mitochondrial
PRO_0000016844Add
BLAST

Proteomic databases

PaxDbiQ9R088.
PRIDEiQ9R088.

PTM databases

PhosphoSiteiQ9R088.

Expressioni

Tissue specificityi

Found in most tissues; highly expressed in liver.

Gene expression databases

CleanExiMM_TK2.
GenevestigatoriQ9R088.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliQ9R088.
SMRiQ9R088. Positions 58-247.

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1428.
HOGENOMiHOG000006872.
HOVERGENiHBG006216.
InParanoidiQ9R088.
PhylomeDBiQ9R088.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R088-1 [UniParc]FASTAAdd to Basket

« Hide

MLLRSLRSWA ARSPRSVGPG SSGSPGSLDS GAGPLWAPRR AWPPDKDREN    50
DKEKKAVVCI EGNIASGKTT CLEFFSNTTD VEVLMEPVLK WRNVHGHNPL 100
SLMYHDASRW GLTLQTYVQL TMLDQHTRPQ MSPVRLMERS IYSARYIFVE 150
NLYRGGKMPE VDYAILSEWF DWIVRNIDVS VDLIVYLRTT PEICYQRLKM 200
RCREEEKVIP MEYLHAIHRL YEEWLVNGSL FPAAAPVLVI EADHNLEKML 250
ELFEQNRARI LTPENWKHGP 270
Length:270
Mass (Da):31,209
Last modified:January 24, 2001 - v2
Checksum:i886F5B80D2C3EFE2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141P → L in AAF08104. 1 Publication
Sequence conflicti23 – 231G → R in AAF08104. 1 Publication
Sequence conflicti155 – 1551G → S in AAF08104. 1 Publication
Sequence conflicti269 – 2702GP → WTLGLSDLQDSARNSPARAR CHGPRA1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF105217 mRNA. Translation: AAF08104.1.
AJ249341 mRNA. Translation: CAC07190.2.
CCDSiCCDS22573.1.
UniGeneiMm.183110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF105217 mRNA. Translation: AAF08104.1 .
AJ249341 mRNA. Translation: CAC07190.2 .
CCDSi CCDS22573.1.
UniGenei Mm.183110.

3D structure databases

ProteinModelPortali Q9R088.
SMRi Q9R088. Positions 58-247.
ModBasei Search...

Chemistry

BindingDBi Q9R088.

PTM databases

PhosphoSitei Q9R088.

Proteomic databases

PaxDbi Q9R088.
PRIDEi Q9R088.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:1913266. Tk2.

Phylogenomic databases

eggNOGi COG1428.
HOGENOMi HOG000006872.
HOVERGENi HBG006216.
InParanoidi Q9R088.
PhylomeDBi Q9R088.

Enzyme and pathway databases

BRENDAi 2.7.1.21. 3474.
SABIO-RK Q9R088.

Miscellaneous databases

PROi Q9R088.
SOURCEi Search...

Gene expression databases

CleanExi MM_TK2.
Genevestigatori Q9R088.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10513. PTHR10513. 1 hit.
Pfami PF01712. dNK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000705. DNK. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of mouse mitochondrial thymidine kinase 2 cDNA."
    Wettin K., Johansson M., Zheng X., Zhu C., Karlsson A.
    FEBS Lett. 460:103-106(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of full length mouse thymidine kinase 2: the N -terminal sequence directs import of the precursor protein into mitochondria."
    Wang L., Eriksson S.
    Biochem. J. 351:469-476(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiKITM_MOUSE
AccessioniPrimary (citable) accession number: Q9R088
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: September 3, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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