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Protein

Glypican-6

Gene

Gpc6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
R-MMU-2022928. HS-GAG biosynthesis.
R-MMU-2024096. HS-GAG degradation.
R-MMU-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Glypican-6
Cleaved into the following chain:
Gene namesi
Name:Gpc6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1346322. Gpc6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 530507Glypican-6PRO_0000012325Add
BLAST
Chaini24 – ?Secreted glypican-6PRO_0000333852
Propeptidei531 – 55525Removed in mature formSequence analysisPRO_0000012326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi530 – 5301GPI-anchor amidated serineSequence analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Heparan sulfate, Lipoprotein, Proteoglycan

Proteomic databases

MaxQBiQ9R087.
PaxDbiQ9R087.
PRIDEiQ9R087.

Expressioni

Tissue specificityi

In the cartilage growth-plate, gradient of expression with highest levels from the proliferative and pre-hypertrophic zones to lowest, if any, in the hypertrophic zones (at protein level).1 Publication

Developmental stagei

Detected from 7 to 17 dpc. Overall expression clearly diminishes after 13 dpc. Mostly expressed in mesoderm-derived tissues, but also present in some neurectoderm-derived sites. High expression limited to mesenchymal tissues. In 11.5 and 13.5 dpc lungs, mostly restricted to the peribronchial mesenchymal cells. In the aorta and other major blood vessels, found in the subendothelial smooth muscle cell layers. Also expressed in the outflow tract of the heart ventricle, but not in other parts of the heart. At 13.5 dpc, in the developing kidney, detected in the metanephric cap mesenchyme of the cortical region, in the condensing mesenchyme surrounding the ureteric branches. Not detected in the adrenal glands. At 11.5 and 13.5 dpc, expressed weakly in the liver septae, but not in the parenchyme. Very strong expression in both the stomach and intestine, in the submucosal layers, in the condensing splanchnic mesenchyme. At 13.5 dpc, expressed in the mesenchymal cells of pancreas, gonad, mesonephric tissue and genital eminence. At 13.5 dpc, expressed in the thymus. At 11.5 dpc, strong expression in the mesenchyme of the mandibular process, with highest expression in the mesenchymal cell layer just below the oral epithelium. Not detected in the overlying epithelium. At 13.5 dpc, highly expressed in the dental mesenchyme surrounding the epithelial bud and near the top of the lip furrow, as well as in the tongue. At 13.5 dpc, expressed in the cartilage primordia of the ear and snout. Highly expressed in intervertebral disks, but not detected in the notochord and vertebrae, both at 11.5 and 13.5 dpc. Highly expressed in mesenchymal condensations of both the forelimb and hindlimb. Overall low expression in the nervous system. At 11.5 dpc, low expression in the neuroepithelium of the hindbrain, the telencephalic vesicle and neuro-epithelial cells lining the mesencephalic vesicle. At 13.5 dpc, detected in the roof of the neopallial cortex, which gives rise to the future cerebral cortex. Weak expression also observed in the medulla oblongata, the choroid plexus, and the ventral mantle layer of the spinal cord. Stronger expression in the ganglia of the glossopharyngeal nerve. At 11.5 and 13.5 dpc, expressed in the mesenchyme surrounding the olfactory epithelium, but not in the epithelium itself. Similarly expressed in the mesenchymal tissues lining the dorsal root ganglia (perineurium), but not in the ganglia. Expression also observed in a few epithelial cells (ectodermal origin), including, at 11.5 dpc, the ventromedial wall of the otic vesicle and, at 13.5 dpc, the cochlea of the inner ear. In the eye, expressed not only in the neural retina but also the cells that compose the wall of the lens vesicle.1 Publication

Gene expression databases

BgeeiQ9R087.
CleanExiMM_GPC6.
ExpressionAtlasiQ9R087. baseline and differential.
GenevisibleiQ9R087. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000120362.

Structurei

3D structure databases

ProteinModelPortaliQ9R087.
SMRiQ9R087. Positions 27-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glypican family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3821. Eukaryota.
ENOG410XST2. LUCA.
GeneTreeiENSGT00550000074430.
HOGENOMiHOG000253003.
HOVERGENiHBG003464.
InParanoidiQ9R087.
KOiK08112.
OrthoDBiEOG761BTG.
PhylomeDBiQ9R087.

Family and domain databases

InterProiIPR001863. Glypican.
IPR031183. Glypican-6.
IPR019803. Glypican_CS.
[Graphical view]
PANTHERiPTHR10822. PTHR10822. 1 hit.
PTHR10822:SF31. PTHR10822:SF31. 1 hit.
PfamiPF01153. Glypican. 1 hit.
[Graphical view]
PROSITEiPS01207. GLYPICAN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSWIRAVIL PLSGLLLTLP AAADVKARSC SEVRQAYGAK GFSLADIPYQ
60 70 80 90 100
EIAGEHLRIC PQEYTCCTTE MEDKLSQQSK LEFENLVEET SHFVRTTFVS
110 120 130 140 150
RHKKFDEFFR ELLENAEKSL NDMFVRTYGM LYMQNSEVFQ DLFTELKRYY
160 170 180 190 200
TGGNVNLEEM LNDFWARLLE RMFQLINPQY HFSEDYLECV SKYTDQLKPF
210 220 230 240 250
GDVPRKLKIQ VTRAFIAART FVQGLTVGRE VANRVSKVSP TPGCIRALMK
260 270 280 290 300
MLYCPYCRGL PTVRPCNNYC LNVMKGCLAN QADLDTEWNL FIDAMLLVAE
310 320 330 340 350
RLEGPFNIES VMDPIDVKIS EAIMNMQENS MQVSAKVFQG CGQPKPAPAL
360 370 380 390 400
RSARSAPENF NTRFRPYNPE ERPTTAAGTS LDRLVTDIKE KLKLSKKVWS
410 420 430 440 450
ALPYTICKDE RVTAGTSNEE ECWNGHSKAR YLPEIMNDGL TNQINNPEVE
460 470 480 490 500
VDITRPDTFI RQQIMALRVM TNKLKNAYNG NDVNFQDTSD ESSGSGSGSG
510 520 530 540 550
CMDDVCPTEF EFVTTEAPAV DPDRREEESS ASKFSSSLIS WSLVCMVLAL

QRLYR
Length:555
Mass (Da):63,057
Last modified:May 1, 2000 - v1
Checksum:i621AFAFF58A839EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105268 mRNA. Translation: AAD55750.1.
CCDSiCCDS27330.1.
RefSeqiNP_035951.1. NM_011821.3.
UniGeneiMm.440025.

Genome annotation databases

EnsembliENSMUST00000078849; ENSMUSP00000077893; ENSMUSG00000058571.
ENSMUST00000088483; ENSMUSP00000085835; ENSMUSG00000058571.
GeneIDi23888.
KEGGimmu:23888.
UCSCiuc007uyl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105268 mRNA. Translation: AAD55750.1.
CCDSiCCDS27330.1.
RefSeqiNP_035951.1. NM_011821.3.
UniGeneiMm.440025.

3D structure databases

ProteinModelPortaliQ9R087.
SMRiQ9R087. Positions 27-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000120362.

Proteomic databases

MaxQBiQ9R087.
PaxDbiQ9R087.
PRIDEiQ9R087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078849; ENSMUSP00000077893; ENSMUSG00000058571.
ENSMUST00000088483; ENSMUSP00000085835; ENSMUSG00000058571.
GeneIDi23888.
KEGGimmu:23888.
UCSCiuc007uyl.2. mouse.

Organism-specific databases

CTDi10082.
MGIiMGI:1346322. Gpc6.

Phylogenomic databases

eggNOGiKOG3821. Eukaryota.
ENOG410XST2. LUCA.
GeneTreeiENSGT00550000074430.
HOGENOMiHOG000253003.
HOVERGENiHBG003464.
InParanoidiQ9R087.
KOiK08112.
OrthoDBiEOG761BTG.
PhylomeDBiQ9R087.

Enzyme and pathway databases

ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
R-MMU-2022928. HS-GAG biosynthesis.
R-MMU-2024096. HS-GAG degradation.
R-MMU-975634. Retinoid metabolism and transport.

Miscellaneous databases

ChiTaRSiGpc6. mouse.
NextBioi303639.
PROiQ9R087.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R087.
CleanExiMM_GPC6.
ExpressionAtlasiQ9R087. baseline and differential.
GenevisibleiQ9R087. MM.

Family and domain databases

InterProiIPR001863. Glypican.
IPR031183. Glypican-6.
IPR019803. Glypican_CS.
[Graphical view]
PANTHERiPTHR10822. PTHR10822. 1 hit.
PTHR10822:SF31. PTHR10822:SF31. 1 hit.
PfamiPF01153. Glypican. 1 hit.
[Graphical view]
PROSITEiPS01207. GLYPICAN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Glypican-6, a new member of the glypican family of cell surface heparan sulfate proteoglycans."
    Veugelers M., De Cat B., Ceulemans H., Bruystens A.-M., Coomans C., Duerr J., Vermeesch J., Marynen P., David G.
    J. Biol. Chem. 274:26968-26977(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: Swiss Webster / NIH.
    Tissue: Embryo.
  2. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  3. "Mutations in the heparan-sulfate proteoglycan glypican 6 (GPC6) impair endochondral ossification and cause recessive omodysplasia."
    Campos-Xavier A.B., Martinet D., Bateman J., Belluoccio D., Rowley L., Tan T.Y., Baxova A., Gustavson K.H., Borochowitz Z.U., Innes A.M., Unger S., Beckmann J.S., Mittaz L., Ballhausen D., Superti-Furga A., Savarirayan R., Bonafe L.
    Am. J. Hum. Genet. 84:760-770(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGPC6_MOUSE
AccessioniPrimary (citable) accession number: Q9R087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.