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Q9R064

- GORS2_RAT

UniProt

Q9R064 - GORS2_RAT

Protein

Golgi reassembly-stacking protein 2

Gene

Gorasp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane Tgfa.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. Golgi organization Source: RGD
    2. organelle organization Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgi reassembly-stacking protein 2
    Short name:
    GRS2
    Alternative name(s):
    Golgi reassembly-stacking protein of 55 kDa
    Short name:
    GRASP55
    Gene namesi
    Name:Gorasp2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619911. Gorasp2.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane 1 Publication; Lipid-anchor 1 Publication. Membrane By similarity; Peripheral membrane protein By similarity
    Note: Intermediate Golgi, membrane-associated.

    GO - Cellular componenti

    1. Golgi apparatus Source: MGI
    2. Golgi cisterna membrane Source: UniProtKB-SubCell
    3. Golgi medial cisterna Source: RGD

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi2 – 21G → A: No or few Golgi targeting, accumulates in the cytoplasm. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedCurated
    Chaini2 – 454453Golgi reassembly-stacking protein 2PRO_0000087547Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineCurated
    Modified residuei30 – 301Omega-N-methylated arginine1 Publication
    Modified residuei47 – 471Omega-N-methylated arginine1 Publication
    Modified residuei214 – 2141PhosphoserineBy similarity
    Modified residuei222 – 2221PhosphothreonineBy similarity
    Modified residuei225 – 2251Phosphothreonine; by MAPKBy similarity
    Modified residuei435 – 4351PhosphothreonineBy similarity
    Modified residuei451 – 4511PhosphoserineBy similarity

    Post-translational modificationi

    Myristoylated. Myristoylation is essential for the Golgi targeting.
    Palmitoylated.By similarity
    Phosphorylated in mitotic cells.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ9R064.
    PRIDEiQ9R064.

    PTM databases

    PhosphoSiteiQ9R064.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    GenevestigatoriQ9R064.

    Interactioni

    Subunit structurei

    Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130. Interacts with KCTD5 By similarity. Interacts with TMED2 and TMED3.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Blzf1Q6AYB85EBI-4422912,EBI-4422894
    MMP14P5028114EBI-4422912,EBI-992788From a different organism.
    Tmed10Q635842EBI-4422912,EBI-918648
    Tmed2Q635244EBI-4422912,EBI-918600
    Tmed9Q5I0E73EBI-4422912,EBI-920903

    Protein-protein interaction databases

    IntActiQ9R064. 17 interactions.
    MINTiMINT-4577436.

    Structurei

    Secondary structure

    1
    454
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi14 – 229
    Helixi27 – 304
    Turni35 – 373
    Beta strandi38 – 436
    Beta strandi50 – 534
    Helixi54 – 618
    Turni62 – 643
    Beta strandi67 – 737
    Turni74 – 763
    Beta strandi79 – 846
    Beta strandi88 – 969
    Beta strandi98 – 1047
    Turni106 – 1105
    Beta strandi113 – 1186
    Helixi123 – 1275
    Turni131 – 1333
    Beta strandi134 – 1407
    Helixi149 – 1557
    Turni156 – 1583
    Beta strandi161 – 1677
    Turni168 – 1714
    Beta strandi172 – 1787
    Beta strandi184 – 19714
    Beta strandi207 – 2104

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4KFWX-ray2.70A/B1-215[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7571PDZAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni194 – 1996Important for membrane bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi278 – 377100Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GORASP family.Curated
    Contains 1 PDZ (DHR) domain.Curated

    Phylogenomic databases

    eggNOGiCOG5233.
    HOGENOMiHOG000231920.
    HOVERGENiHBG051826.
    InParanoidiQ9R064.
    PhylomeDBiQ9R064.

    Family and domain databases

    Gene3Di2.30.42.10. 1 hit.
    InterProiIPR024958. GRASP55/65_PDZ.
    IPR007583. GRASP55_65.
    IPR001478. PDZ.
    [Graphical view]
    PANTHERiPTHR12893. PTHR12893. 1 hit.
    PfamiPF04495. GRASP55_65. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9R064-1 [UniParc]FASTAAdd to Basket

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    MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSISGSRLNK    50
    DNDTLKDLLK ANVEKPVKML IYSSKTLELR EASVTPSNLW GGQGLLGVSI 100
    RFCSFDGANE NVWHVLEVES NSPAALAGLR PHSDYIIGAD TVMNESEDLF 150
    SLIETHEAKP LKLYVYNTDT DNCREVIITP NSAWGGEGSL GCGIGYGYLH 200
    RIPTRPFEEG KKISLPGQMT GTPITPLKDG FTQVQLSSVS PPSLSPPGTA 250
    GVEQSLSGLS ISSAPPAVSN VLSTGVPTVP LLPPQVNQSL ASVPPMNPAA 300
    TLPSLMPLSA GLPNLPNLPS LSNFNLPAPH IMPGVGLPEL GKPGLPPLPS 350
    LPPRNVPGIA PLPMPSDFLP SFPLVPEGSS AASAGEPLSS LPAMGPPSDP 400
    VMTTAKADTS SLTVDVMSPA SKVPTTVEDR VSDCTPAMEK PVSAVTDANA 450
    SGAS 454
    Length:454
    Mass (Da):47,221
    Last modified:January 23, 2007 - v3
    Checksum:iBB6898C85CB6221C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF110267 mRNA. Translation: AAD55350.1.
    UniGeneiRn.145137.

    Genome annotation databases

    UCSCiRGD:619911. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF110267 mRNA. Translation: AAD55350.1 .
    UniGenei Rn.145137.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4KFW X-ray 2.70 A/B 1-215 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9R064. 17 interactions.
    MINTi MINT-4577436.

    PTM databases

    PhosphoSitei Q9R064.

    Proteomic databases

    PaxDbi Q9R064.
    PRIDEi Q9R064.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:619911. rat.

    Organism-specific databases

    RGDi 619911. Gorasp2.

    Phylogenomic databases

    eggNOGi COG5233.
    HOGENOMi HOG000231920.
    HOVERGENi HBG051826.
    InParanoidi Q9R064.
    PhylomeDBi Q9R064.

    Miscellaneous databases

    PROi Q9R064.

    Gene expression databases

    Genevestigatori Q9R064.

    Family and domain databases

    Gene3Di 2.30.42.10. 1 hit.
    InterProi IPR024958. GRASP55/65_PDZ.
    IPR007583. GRASP55_65.
    IPR001478. PDZ.
    [Graphical view ]
    PANTHERi PTHR12893. PTHR12893. 1 hit.
    Pfami PF04495. GRASP55_65. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system."
      Shorter J., Watson R., Giannakou M.-E., Clarke M., Warren G., Barr F.A.
      EMBO J. 18:4949-4960(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus."
      Barr F.A., Preisinger C., Kopajtich R., Koerner R.
      J. Cell Biol. 155:885-891(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TMED2 AND TMED3.
    3. Cited for: METHYLATION AT ARG-30 AND ARG-47, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGORS2_RAT
    AccessioniPrimary (citable) accession number: Q9R064
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3