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Q9R064 (GORS2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi reassembly-stacking protein 2

Short name=GRS2
Alternative name(s):
Golgi reassembly-stacking protein of 55 kDa
Short name=GRASP55
Gene names
Name:Gorasp2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane Tgfa.

Subunit structure

Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130. Interacts with KCTD5 By similarity. Interacts with TMED2 and TMED3. Ref.2

Subcellular location

Golgi apparatusGolgi stack membrane; Lipid-anchor. Membrane; Peripheral membrane protein By similarity. Note: Intermediate Golgi, membrane-associated. Ref.1

Tissue specificity

Ubiquitous. Ref.1

Post-translational modification

Myristoylated. Myristoylation is essential for the Golgi targeting.

Palmitoylated By similarity.

Phosphorylated in mitotic cells By similarity.

Sequence similarities

Belongs to the GORASP family.

Contains 1 PDZ (DHR) domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Probable
Chain2 – 454453Golgi reassembly-stacking protein 2
PRO_0000087547

Regions

Domain5 – 7571PDZ
Region194 – 1996Important for membrane binding By similarity
Compositional bias278 – 377100Pro-rich

Amino acid modifications

Modified residue301Omega-N-methylated arginine Ref.3
Modified residue471Omega-N-methylated arginine Ref.3
Modified residue2141Phosphoserine By similarity
Modified residue2221Phosphothreonine By similarity
Modified residue2251Phosphothreonine; by MAPK By similarity
Modified residue4351Phosphothreonine By similarity
Modified residue4511Phosphoserine By similarity
Lipidation21N-myristoyl glycine Probable

Experimental info

Mutagenesis21G → A: No or few Golgi targeting, accumulates in the cytoplasm.

Secondary structure

......................................... 454
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9R064 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: BB6898C85CB6221C

FASTA45447,221
        10         20         30         40         50         60 
MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSISGSRLNK DNDTLKDLLK 

        70         80         90        100        110        120 
ANVEKPVKML IYSSKTLELR EASVTPSNLW GGQGLLGVSI RFCSFDGANE NVWHVLEVES 

       130        140        150        160        170        180 
NSPAALAGLR PHSDYIIGAD TVMNESEDLF SLIETHEAKP LKLYVYNTDT DNCREVIITP 

       190        200        210        220        230        240 
NSAWGGEGSL GCGIGYGYLH RIPTRPFEEG KKISLPGQMT GTPITPLKDG FTQVQLSSVS 

       250        260        270        280        290        300 
PPSLSPPGTA GVEQSLSGLS ISSAPPAVSN VLSTGVPTVP LLPPQVNQSL ASVPPMNPAA 

       310        320        330        340        350        360 
TLPSLMPLSA GLPNLPNLPS LSNFNLPAPH IMPGVGLPEL GKPGLPPLPS LPPRNVPGIA 

       370        380        390        400        410        420 
PLPMPSDFLP SFPLVPEGSS AASAGEPLSS LPAMGPPSDP VMTTAKADTS SLTVDVMSPA 

       430        440        450 
SKVPTTVEDR VSDCTPAMEK PVSAVTDANA SGAS 

« Hide

References

[1]"GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system."
Shorter J., Watson R., Giannakou M.-E., Clarke M., Warren G., Barr F.A.
EMBO J. 18:4949-4960(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: Sprague-Dawley.
Tissue: Testis.
[2]"Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus."
Barr F.A., Preisinger C., Kopajtich R., Koerner R.
J. Cell Biol. 155:885-891(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TMED2 AND TMED3.
[3]"Organellar proteomics reveals Golgi arginine dimethylation."
Wu C.C., MacCoss M.J., Mardones G., Finnigan C., Mogelsvang S., Yates J.R. III, Howell K.E.
Mol. Biol. Cell 15:2907-2919(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT ARG-30 AND ARG-47, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF110267 mRNA. Translation: AAD55350.1.
UniGeneRn.145137.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4KFWX-ray2.70A/B1-215[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9R064. 17 interactions.
MINTMINT-4577436.

PTM databases

PhosphoSiteQ9R064.

Proteomic databases

PaxDbQ9R064.
PRIDEQ9R064.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:619911. rat.

Organism-specific databases

RGD619911. Gorasp2.

Phylogenomic databases

eggNOGCOG5233.
HOGENOMHOG000231920.
HOVERGENHBG051826.
InParanoidQ9R064.
PhylomeDBQ9R064.

Gene expression databases

GenevestigatorQ9R064.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERPTHR12893. PTHR12893. 1 hit.
PfamPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 2 hits.
ProtoNetSearch...

Other

PROQ9R064.

Entry information

Entry nameGORS2_RAT
AccessionPrimary (citable) accession number: Q9R064
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references