Q9R064 (GORS2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi reassembly-stacking protein 2
Short name=GRS2

Alternative name(s):
Golgi reassembly-stacking protein of 55 kDa
Short name=GRASP55

Gene names

Name:

Gorasp2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane Tgfa.
Subunit structure	Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130. Interacts with KCTD5 By similarity. Interacts with TMED2 and TMED3. Ref.2
Subcellular location	Golgi apparatus › Golgi stack membrane; Lipid-anchor. Membrane; Peripheral membrane protein By similarity. Note: Intermediate Golgi, membrane-associated. Ref.1
Tissue specificity	Ubiquitous. Ref.1
Post-translational modification	Myristoylated. Myristoylation is essential for the Golgi targeting. Palmitoylated By similarity. Phosphorylated in mitotic cells By similarity.
Sequence similarities	Belongs to the GORASP family. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
PTM	Lipoprotein Methylation Myristate Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from direct assay Ref.1. Source: RGD
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi medial cisterna Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Blzf1	Q6AYB8	5	EBI-4422912,EBI-4422894
Tmed10	Q63584	2	EBI-4422912,EBI-918648
Tmed2	Q63524	4	EBI-4422912,EBI-918600
Tmed9	Q5I0E7	3	EBI-4422912,EBI-920903

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Golgi reassembly-stacking protein 2		PRO_0000087547
Regions
	Domain	5 – 75	71	PDZ
	Region	194 – 199	6	Important for membrane binding By similarity
	Compositional bias	278 – 377	100	Pro-rich
Amino acid modifications
	Modified residue	30	1	Omega-N-methylated arginine Ref.3
	Modified residue	47	1	Omega-N-methylated arginine Ref.3
	Modified residue	214	1	Phosphoserine By similarity
	Modified residue	222	1	Phosphothreonine By similarity
	Modified residue	225	1	Phosphothreonine; by MAPK By similarity
	Modified residue	411	1	Phosphoserine By similarity
	Modified residue	432	1	Phosphoserine By similarity
	Modified residue	435	1	Phosphothreonine By similarity
	Modified residue	451	1	Phosphoserine By similarity
	Lipidation	2	1	N-myristoyl glycine Probable
Experimental info
	Mutagenesis	2	1	G → A: No or few Golgi targeting, accumulates in the cytoplasm.

Sequences

	Sequence		Length	Mass (Da)
	Q9R064 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BB6898C85CB6221C	FASTA	454	47,221
	10 20 30 40 50 60 MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSISGSRLNK DNDTLKDLLK 70 80 90 100 110 120 ANVEKPVKML IYSSKTLELR EASVTPSNLW GGQGLLGVSI RFCSFDGANE NVWHVLEVES 130 140 150 160 170 180 NSPAALAGLR PHSDYIIGAD TVMNESEDLF SLIETHEAKP LKLYVYNTDT DNCREVIITP 190 200 210 220 230 240 NSAWGGEGSL GCGIGYGYLH RIPTRPFEEG KKISLPGQMT GTPITPLKDG FTQVQLSSVS 250 260 270 280 290 300 PPSLSPPGTA GVEQSLSGLS ISSAPPAVSN VLSTGVPTVP LLPPQVNQSL ASVPPMNPAA 310 320 330 340 350 360 TLPSLMPLSA GLPNLPNLPS LSNFNLPAPH IMPGVGLPEL GKPGLPPLPS LPPRNVPGIA 370 380 390 400 410 420 PLPMPSDFLP SFPLVPEGSS AASAGEPLSS LPAMGPPSDP VMTTAKADTS SLTVDVMSPA 430 440 450 SKVPTTVEDR VSDCTPAMEK PVSAVTDANA SGAS

References

[1]	"GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system." Shorter J., Watson R., Giannakou M.-E., Clarke M., Warren G., Barr F.A. EMBO J. 18:4949-4960(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: Sprague-Dawley. Tissue: Testis.
[2]	"Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus." Barr F.A., Preisinger C., Kopajtich R., Koerner R. J. Cell Biol. 155:885-891(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TMED2 AND TMED3.
[3]	"Organellar proteomics reveals Golgi arginine dimethylation." Wu C.C., MacCoss M.J., Mardones G., Finnigan C., Mogelsvang S., Yates J.R. III, Howell K.E. Mol. Biol. Cell 15:2907-2919(2004) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT ARG-30 AND ARG-47, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF110267 mRNA. Translation: AAD55350.1.
IPI	IPI00480782.
UniGene	Rn.145137.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9R064. 9 interactions.
PTM databases
PhosphoSite	Q9R064.
Proteomic databases
PaxDb	Q9R064.
PRIDE	Q9R064.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:619911. rat.
Organism-specific databases
RGD	619911. Gorasp2.
Phylogenomic databases
eggNOG	COG5233.
HOGENOM	HOG000231920.
HOVERGEN	HBG051826.
InParanoid	Q9R064.
OrthoDB	EOG4PVP04.
Gene expression databases
ArrayExpress	Q9R064.
Genevestigator	Q9R064.
GermOnline	ENSRNOG00000023386. Rattus norvegicus.
Family and domain databases
InterPro	IPR024958. GRASP55/65_PDZ. IPR007583. GRASP55_65. IPR001478. PDZ. [Graphical view]
PANTHER	PTHR12893. PTHR12893. 1 hit.
Pfam	PF04495. GRASP55_65. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 2 hits.
PROSITE	PS50106. PDZ. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GORS2_RAT

Accession

Primary (citable) accession number: Q9R064

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program