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Protein

Golgi reassembly-stacking protein 2

Gene

Gorasp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane Tgfa.

GO - Biological processi

  • Golgi organization Source: RGD
  • organelle organization Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 2
Short name:
GRS2
Alternative name(s):
Golgi reassembly-stacking protein of 55 kDa
Short name:
GRASP55
Gene namesi
Name:Gorasp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619911. Gorasp2.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: MGI
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • Golgi medial cisterna Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: No or few Golgi targeting, accumulates in the cytoplasm. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000875472 – 454Golgi reassembly-stacking protein 2Add BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei30Dimethylated arginine1 Publication1
Modified residuei47Dimethylated arginine1 Publication1
Modified residuei214PhosphoserineBy similarity1
Modified residuei222PhosphothreonineBy similarity1
Modified residuei225Phosphothreonine; by MAPKBy similarity1
Modified residuei411PhosphoserineBy similarity1
Modified residuei435PhosphothreonineBy similarity1
Modified residuei443PhosphoserineBy similarity1
Modified residuei451PhosphoserineBy similarity1

Post-translational modificationi

Myristoylated. Myristoylation is essential for the Golgi targeting.
Palmitoylated.By similarity
Phosphorylated in mitotic cells.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9R064.
PRIDEiQ9R064.

PTM databases

iPTMnetiQ9R064.
PhosphoSitePlusiQ9R064.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Interactioni

Subunit structurei

Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130. Interacts with KCTD5 (By similarity). Interacts with TMED2 and TMED3.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Blzf1Q6AYB85EBI-4422912,EBI-4422894
MMP14P5028114EBI-4422912,EBI-992788From a different organism.
Tmed10Q635842EBI-4422912,EBI-918648
Tmed2Q635244EBI-4422912,EBI-918600
Tmed9Q5I0E73EBI-4422912,EBI-920903

Protein-protein interaction databases

IntActiQ9R064. 17 interactors.
MINTiMINT-4577436.
STRINGi10116.ENSRNOP00000036881.

Structurei

Secondary structure

1454
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 22Combined sources9
Helixi27 – 30Combined sources4
Turni35 – 37Combined sources3
Beta strandi38 – 43Combined sources6
Beta strandi50 – 53Combined sources4
Helixi54 – 61Combined sources8
Turni62 – 64Combined sources3
Beta strandi67 – 73Combined sources7
Turni74 – 76Combined sources3
Beta strandi79 – 84Combined sources6
Beta strandi88 – 96Combined sources9
Beta strandi98 – 104Combined sources7
Turni106 – 110Combined sources5
Beta strandi113 – 118Combined sources6
Helixi123 – 127Combined sources5
Turni131 – 133Combined sources3
Beta strandi134 – 140Combined sources7
Helixi149 – 155Combined sources7
Turni156 – 158Combined sources3
Beta strandi161 – 167Combined sources7
Turni168 – 171Combined sources4
Beta strandi172 – 178Combined sources7
Beta strandi184 – 197Combined sources14
Beta strandi207 – 210Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KFWX-ray2.70A/B1-215[»]
SMRiQ9R064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 75PDZAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 199Important for membrane bindingBy similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 377Pro-richAdd BLAST100

Sequence similaritiesi

Belongs to the GORASP family.Curated
Contains 1 PDZ (DHR) domain.Curated

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
HOGENOMiHOG000231920.
HOVERGENiHBG051826.
InParanoidiQ9R064.
PhylomeDBiQ9R064.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSISGSRLNK
60 70 80 90 100
DNDTLKDLLK ANVEKPVKML IYSSKTLELR EASVTPSNLW GGQGLLGVSI
110 120 130 140 150
RFCSFDGANE NVWHVLEVES NSPAALAGLR PHSDYIIGAD TVMNESEDLF
160 170 180 190 200
SLIETHEAKP LKLYVYNTDT DNCREVIITP NSAWGGEGSL GCGIGYGYLH
210 220 230 240 250
RIPTRPFEEG KKISLPGQMT GTPITPLKDG FTQVQLSSVS PPSLSPPGTA
260 270 280 290 300
GVEQSLSGLS ISSAPPAVSN VLSTGVPTVP LLPPQVNQSL ASVPPMNPAA
310 320 330 340 350
TLPSLMPLSA GLPNLPNLPS LSNFNLPAPH IMPGVGLPEL GKPGLPPLPS
360 370 380 390 400
LPPRNVPGIA PLPMPSDFLP SFPLVPEGSS AASAGEPLSS LPAMGPPSDP
410 420 430 440 450
VMTTAKADTS SLTVDVMSPA SKVPTTVEDR VSDCTPAMEK PVSAVTDANA

SGAS
Length:454
Mass (Da):47,221
Last modified:January 23, 2007 - v3
Checksum:iBB6898C85CB6221C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110267 mRNA. Translation: AAD55350.1.
UniGeneiRn.145137.

Genome annotation databases

UCSCiRGD:619911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110267 mRNA. Translation: AAD55350.1.
UniGeneiRn.145137.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KFWX-ray2.70A/B1-215[»]
SMRiQ9R064.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R064. 17 interactors.
MINTiMINT-4577436.
STRINGi10116.ENSRNOP00000036881.

PTM databases

iPTMnetiQ9R064.
PhosphoSitePlusiQ9R064.

Proteomic databases

PaxDbiQ9R064.
PRIDEiQ9R064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:619911. rat.

Organism-specific databases

RGDi619911. Gorasp2.

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
HOGENOMiHOG000231920.
HOVERGENiHBG051826.
InParanoidiQ9R064.
PhylomeDBiQ9R064.

Miscellaneous databases

PROiQ9R064.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGORS2_RAT
AccessioniPrimary (citable) accession number: Q9R064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.