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Q9R064

- GORS2_RAT

UniProt

Q9R064 - GORS2_RAT

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Protein
Golgi reassembly-stacking protein 2
Gene
Gorasp2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane Tgfa.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. Golgi organization Source: RGD
  2. organelle organization Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 2
Short name:
GRS2
Alternative name(s):
Golgi reassembly-stacking protein of 55 kDa
Short name:
GRASP55
Gene namesi
Name:Gorasp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619911. Gorasp2.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Lipid-anchor. Membrane; Peripheral membrane protein By similarity
Note: Intermediate Golgi, membrane-associated.1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. Golgi cisterna membrane Source: UniProtKB-SubCell
  3. Golgi medial cisterna Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: No or few Golgi targeting, accumulates in the cytoplasm.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Inferred
Chaini2 – 454453Golgi reassembly-stacking protein 2
PRO_0000087547Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine Inferred
Modified residuei30 – 301Omega-N-methylated arginine1 Publication
Modified residuei47 – 471Omega-N-methylated arginine1 Publication
Modified residuei214 – 2141Phosphoserine By similarity
Modified residuei222 – 2221Phosphothreonine By similarity
Modified residuei225 – 2251Phosphothreonine; by MAPK By similarity
Modified residuei435 – 4351Phosphothreonine By similarity
Modified residuei451 – 4511Phosphoserine By similarity

Post-translational modificationi

Myristoylated. Myristoylation is essential for the Golgi targeting.
Palmitoylated By similarity.
Phosphorylated in mitotic cells By similarity.

Keywords - PTMi

Lipoprotein, Methylation, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9R064.
PRIDEiQ9R064.

PTM databases

PhosphoSiteiQ9R064.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

GenevestigatoriQ9R064.

Interactioni

Subunit structurei

Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130. Interacts with KCTD5 By similarity. Interacts with TMED2 and TMED3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Blzf1Q6AYB85EBI-4422912,EBI-4422894
MMP14P5028114EBI-4422912,EBI-992788From a different organism.
Tmed10Q635842EBI-4422912,EBI-918648
Tmed2Q635244EBI-4422912,EBI-918600
Tmed9Q5I0E73EBI-4422912,EBI-920903

Protein-protein interaction databases

IntActiQ9R064. 17 interactions.
MINTiMINT-4577436.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 229
Helixi27 – 304
Turni35 – 373
Beta strandi38 – 436
Beta strandi50 – 534
Helixi54 – 618
Turni62 – 643
Beta strandi67 – 737
Turni74 – 763
Beta strandi79 – 846
Beta strandi88 – 969
Beta strandi98 – 1047
Turni106 – 1105
Beta strandi113 – 1186
Helixi123 – 1275
Turni131 – 1333
Beta strandi134 – 1407
Helixi149 – 1557
Turni156 – 1583
Beta strandi161 – 1677
Turni168 – 1714
Beta strandi172 – 1787
Beta strandi184 – 19714
Beta strandi207 – 2104

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KFWX-ray2.70A/B1-215[»]

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7571PDZ
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1996Important for membrane binding By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 377100Pro-rich
Add
BLAST

Sequence similaritiesi

Belongs to the GORASP family.
Contains 1 PDZ (DHR) domain.

Phylogenomic databases

eggNOGiCOG5233.
HOGENOMiHOG000231920.
HOVERGENiHBG051826.
InParanoidiQ9R064.
PhylomeDBiQ9R064.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R064-1 [UniParc]FASTAAdd to Basket

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MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSISGSRLNK    50
DNDTLKDLLK ANVEKPVKML IYSSKTLELR EASVTPSNLW GGQGLLGVSI 100
RFCSFDGANE NVWHVLEVES NSPAALAGLR PHSDYIIGAD TVMNESEDLF 150
SLIETHEAKP LKLYVYNTDT DNCREVIITP NSAWGGEGSL GCGIGYGYLH 200
RIPTRPFEEG KKISLPGQMT GTPITPLKDG FTQVQLSSVS PPSLSPPGTA 250
GVEQSLSGLS ISSAPPAVSN VLSTGVPTVP LLPPQVNQSL ASVPPMNPAA 300
TLPSLMPLSA GLPNLPNLPS LSNFNLPAPH IMPGVGLPEL GKPGLPPLPS 350
LPPRNVPGIA PLPMPSDFLP SFPLVPEGSS AASAGEPLSS LPAMGPPSDP 400
VMTTAKADTS SLTVDVMSPA SKVPTTVEDR VSDCTPAMEK PVSAVTDANA 450
SGAS 454
Length:454
Mass (Da):47,221
Last modified:January 23, 2007 - v3
Checksum:iBB6898C85CB6221C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF110267 mRNA. Translation: AAD55350.1.
UniGeneiRn.145137.

Genome annotation databases

UCSCiRGD:619911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF110267 mRNA. Translation: AAD55350.1 .
UniGenei Rn.145137.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4KFW X-ray 2.70 A/B 1-215 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9R064. 17 interactions.
MINTi MINT-4577436.

PTM databases

PhosphoSitei Q9R064.

Proteomic databases

PaxDbi Q9R064.
PRIDEi Q9R064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:619911. rat.

Organism-specific databases

RGDi 619911. Gorasp2.

Phylogenomic databases

eggNOGi COG5233.
HOGENOMi HOG000231920.
HOVERGENi HBG051826.
InParanoidi Q9R064.
PhylomeDBi Q9R064.

Miscellaneous databases

PROi Q9R064.

Gene expression databases

Genevestigatori Q9R064.

Family and domain databases

Gene3Di 2.30.42.10. 1 hit.
InterProi IPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view ]
PANTHERi PTHR12893. PTHR12893. 1 hit.
Pfami PF04495. GRASP55_65. 1 hit.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system."
    Shorter J., Watson R., Giannakou M.-E., Clarke M., Warren G., Barr F.A.
    EMBO J. 18:4949-4960(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus."
    Barr F.A., Preisinger C., Kopajtich R., Koerner R.
    J. Cell Biol. 155:885-891(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TMED2 AND TMED3.
  3. Cited for: METHYLATION AT ARG-30 AND ARG-47, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGORS2_RAT
AccessioniPrimary (citable) accession number: Q9R064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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