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Q9R064

- GORS2_RAT

UniProt

Q9R064 - GORS2_RAT

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Protein

Golgi reassembly-stacking protein 2

Gene

Gorasp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane Tgfa.

GO - Biological processi

  1. Golgi organization Source: RGD
  2. organelle organization Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 2
Short name:
GRS2
Alternative name(s):
Golgi reassembly-stacking protein of 55 kDa
Short name:
GRASP55
Gene namesi
Name:Gorasp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619911. Gorasp2.

Subcellular locationi

Golgi apparatusGolgi stack membrane 1 Publication; Lipid-anchor 1 Publication. Membrane By similarity; Peripheral membrane protein By similarity
Note: Intermediate Golgi, membrane-associated.

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. Golgi medial cisterna Source: RGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: No or few Golgi targeting, accumulates in the cytoplasm. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedCurated
Chaini2 – 454453Golgi reassembly-stacking protein 2PRO_0000087547Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineCurated
Modified residuei30 – 301Dimethylated arginine1 Publication
Modified residuei47 – 471Dimethylated arginine1 Publication
Modified residuei214 – 2141PhosphoserineBy similarity
Modified residuei222 – 2221PhosphothreonineBy similarity
Modified residuei225 – 2251Phosphothreonine; by MAPKBy similarity
Modified residuei435 – 4351PhosphothreonineBy similarity
Modified residuei451 – 4511PhosphoserineBy similarity

Post-translational modificationi

Myristoylated. Myristoylation is essential for the Golgi targeting.
Palmitoylated.By similarity
Phosphorylated in mitotic cells.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9R064.
PRIDEiQ9R064.

PTM databases

PhosphoSiteiQ9R064.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

GenevestigatoriQ9R064.

Interactioni

Subunit structurei

Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130. Interacts with KCTD5 (By similarity). Interacts with TMED2 and TMED3.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Blzf1Q6AYB85EBI-4422912,EBI-4422894
MMP14P5028114EBI-4422912,EBI-992788From a different organism.
Tmed10Q635842EBI-4422912,EBI-918648
Tmed2Q635244EBI-4422912,EBI-918600
Tmed9Q5I0E73EBI-4422912,EBI-920903

Protein-protein interaction databases

IntActiQ9R064. 17 interactions.
MINTiMINT-4577436.

Structurei

Secondary structure

1
454
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 229Combined sources
Helixi27 – 304Combined sources
Turni35 – 373Combined sources
Beta strandi38 – 436Combined sources
Beta strandi50 – 534Combined sources
Helixi54 – 618Combined sources
Turni62 – 643Combined sources
Beta strandi67 – 737Combined sources
Turni74 – 763Combined sources
Beta strandi79 – 846Combined sources
Beta strandi88 – 969Combined sources
Beta strandi98 – 1047Combined sources
Turni106 – 1105Combined sources
Beta strandi113 – 1186Combined sources
Helixi123 – 1275Combined sources
Turni131 – 1333Combined sources
Beta strandi134 – 1407Combined sources
Helixi149 – 1557Combined sources
Turni156 – 1583Combined sources
Beta strandi161 – 1677Combined sources
Turni168 – 1714Combined sources
Beta strandi172 – 1787Combined sources
Beta strandi184 – 19714Combined sources
Beta strandi207 – 2104Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KFWX-ray2.70A/B1-215[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7571PDZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1996Important for membrane bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 377100Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the GORASP family.Curated
Contains 1 PDZ (DHR) domain.Curated

Phylogenomic databases

eggNOGiCOG5233.
HOGENOMiHOG000231920.
HOVERGENiHBG051826.
InParanoidiQ9R064.
PhylomeDBiQ9R064.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R064-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSISGSRLNK
60 70 80 90 100
DNDTLKDLLK ANVEKPVKML IYSSKTLELR EASVTPSNLW GGQGLLGVSI
110 120 130 140 150
RFCSFDGANE NVWHVLEVES NSPAALAGLR PHSDYIIGAD TVMNESEDLF
160 170 180 190 200
SLIETHEAKP LKLYVYNTDT DNCREVIITP NSAWGGEGSL GCGIGYGYLH
210 220 230 240 250
RIPTRPFEEG KKISLPGQMT GTPITPLKDG FTQVQLSSVS PPSLSPPGTA
260 270 280 290 300
GVEQSLSGLS ISSAPPAVSN VLSTGVPTVP LLPPQVNQSL ASVPPMNPAA
310 320 330 340 350
TLPSLMPLSA GLPNLPNLPS LSNFNLPAPH IMPGVGLPEL GKPGLPPLPS
360 370 380 390 400
LPPRNVPGIA PLPMPSDFLP SFPLVPEGSS AASAGEPLSS LPAMGPPSDP
410 420 430 440 450
VMTTAKADTS SLTVDVMSPA SKVPTTVEDR VSDCTPAMEK PVSAVTDANA

SGAS
Length:454
Mass (Da):47,221
Last modified:January 23, 2007 - v3
Checksum:iBB6898C85CB6221C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF110267 mRNA. Translation: AAD55350.1.
UniGeneiRn.145137.

Genome annotation databases

UCSCiRGD:619911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF110267 mRNA. Translation: AAD55350.1 .
UniGenei Rn.145137.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4KFW X-ray 2.70 A/B 1-215 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9R064. 17 interactions.
MINTi MINT-4577436.

PTM databases

PhosphoSitei Q9R064.

Proteomic databases

PaxDbi Q9R064.
PRIDEi Q9R064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:619911. rat.

Organism-specific databases

RGDi 619911. Gorasp2.

Phylogenomic databases

eggNOGi COG5233.
HOGENOMi HOG000231920.
HOVERGENi HBG051826.
InParanoidi Q9R064.
PhylomeDBi Q9R064.

Miscellaneous databases

PROi Q9R064.

Gene expression databases

Genevestigatori Q9R064.

Family and domain databases

Gene3Di 2.30.42.10. 1 hit.
InterProi IPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view ]
PANTHERi PTHR12893. PTHR12893. 1 hit.
Pfami PF04495. GRASP55_65. 1 hit.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system."
    Shorter J., Watson R., Giannakou M.-E., Clarke M., Warren G., Barr F.A.
    EMBO J. 18:4949-4960(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus."
    Barr F.A., Preisinger C., Kopajtich R., Koerner R.
    J. Cell Biol. 155:885-891(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TMED2 AND TMED3.
  3. Cited for: METHYLATION AT ARG-30 AND ARG-47, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGORS2_RAT
AccessioniPrimary (citable) accession number: Q9R064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3