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Protein

Peroxiredoxin-5, mitochondrial

Gene

Prdx5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys Prx, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BRENDAi1.11.1.15. 5301.

Protein family/group databases

PeroxiBasei4452. RnoPrxV.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-5, mitochondrial (EC:1.11.1.15)
Alternative name(s):
Antioxidant enzyme B166
Short name:
AOEB166
PLP
Peroxiredoxin V
Short name:
Prx-V
Peroxisomal antioxidant enzyme
Thioredoxin peroxidase PMP20
Gene namesi
Name:Prdx5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71007. Prdx5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51MitochondrionSequence analysisAdd BLAST51
ChainiPRO_000002379952 – 213Peroxiredoxin-5, mitochondrialAdd BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74N6-acetyllysineBy similarity1
Modified residuei82N6-acetyllysine; alternateBy similarity1
Modified residuei82N6-succinyllysine; alternateBy similarity1
Disulfide bondi99 ↔ 203Redox-activePROSITE-ProRule annotation
Modified residuei115N6-succinyllysineBy similarity1
Modified residuei170PhosphoserineCombined sources1
Modified residuei181PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9R063.
PRIDEiQ9R063.

PTM databases

iPTMnetiQ9R063.
PhosphoSitePlusiQ9R063.
SwissPalmiQ9R063.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi250207. 1 interactor.
IntActiQ9R063. 1 interactor.
STRINGi10116.ENSRNOP00000028687.

Structurei

3D structure databases

ProteinModelPortaliQ9R063.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 213ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi211 – 213Microbody targeting signalBy similarity3

Sequence similaritiesi

Belongs to the peroxiredoxin family. Prx5 subfamily.Curated

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0541. Eukaryota.
COG0678. LUCA.
HOGENOMiHOG000255884.
HOVERGENiHBG053675.
InParanoidiQ9R063.
KOiK11187.
PhylomeDBiQ9R063.
TreeFamiTF105182.

Family and domain databases

InterProiView protein in InterPro
IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF08534. Redoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: Q9R063-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVQLRFCVLG SIAGSVLRAS ATWTCVAGRA GRKGAGWECG GARSFSSAAV
60 70 80 90 100
TMAPIKVGDT IPSVEVFEGE PGKKVNLAEL FKDKKGVLFG VPGAFTPGCS
110 120 130 140 150
KTHLPGFVEQ AGALKAKGAQ VVACLSVNDA FVTAEWGRAH QAEGKVQLLA
160 170 180 190 200
DPTGAFGKET DLLLDDSLVS LFGNRRLKRF SMVIDKGVVK ALNVEPDGTG
210
LTCSLAPNIL SQL
Length:213
Mass (Da):22,179
Last modified:May 1, 2000 - v1
Checksum:i9F0D03A4CC87708A
GO
Isoform Cytoplasmic+peroxisomal (identifier: Q9R063-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Show »
Length:162
Mass (Da):17,035
Checksum:i4230B34E574E2654
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti68E → G. 1
Natural varianti114L → P. 1
Natural varianti130A → V1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188321 – 51Missing in isoform Cytoplasmic+peroxisomal. CuratedAdd BLAST51

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110732 mRNA. Translation: AAF03751.1.
BC078771 mRNA. Translation: AAH78771.1.
RefSeqiNP_446062.1. NM_053610.1. [Q9R063-1]
UniGeneiRn.2944.

Genome annotation databases

GeneIDi113898.
KEGGirno:113898.
UCSCiRGD:71007. rat. [Q9R063-1]

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPRDX5_RAT
AccessioniPrimary (citable) accession number: Q9R063
Secondary accession number(s): Q68G22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families