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Protein

Cytosolic Fe-S cluster assembly factor NUBP2

Gene

Nubp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure (PubMed:23807208).UniRule annotation1 Publication

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi200 – 2001Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi203 – 2031Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP2UniRule annotation
Alternative name(s):
Nucleotide-binding protein 2UniRule annotation
Short name:
NBP 2UniRule annotation
Gene namesi
Name:Nubp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1347072. Nubp2.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: UniProtKB-SubCell
  • cilium Source: UniProtKB-KW
  • nucleus Source: MGI
  • spindle pole centrosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Cytosolic Fe-S cluster assembly factor NUBP2PRO_0000184946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineUniRule annotationBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9R061.
MaxQBiQ9R061.
PaxDbiQ9R061.
PeptideAtlasiQ9R061.
PRIDEiQ9R061.

PTM databases

PhosphoSiteiQ9R061.

Expressioni

Tissue specificityi

Widely expressed.

Developmental stagei

Expressed at 7, 11, 15 and 17 dpc.

Gene expression databases

BgeeiENSMUSG00000039183.
CleanExiMM_NUBP2.
GenevisibleiQ9R061. MM.

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (By similarity). Interacts with KIFC1 (PubMed:16638812). Interacts with NUBP1 (PubMed:16638812, PubMed:23807208).UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049319.

Structurei

3D structure databases

ProteinModelPortaliQ9R061.
SMRiQ9R061. Positions 21-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00550000075057.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ9R061.
OMAiSHLGADP.
OrthoDBiEOG091G0DY5.
PhylomeDBiQ9R061.
TreeFamiTF354321.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03039. NUBP2. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAAAGERAE PGNLAGVRHI ILVLSGKGGV GKSTISTELA LALRHQGKKV
60 70 80 90 100
GILDVDLCGP SIPHMLRAQG KAVHQCDNGW VPVFVDQEQS ISLMSVGFLL
110 120 130 140 150
ENPDEAVVWR GPKKHALIKQ FVSDVAWGQL DYLVVDTPPG TSDEHMATME
160 170 180 190 200
ALRPYRPLGA LVVTTPQAVS IGDVRRELTF CKKTGLQVIG VIENMSGFTC
210 220 230 240 250
PHCAECTNVF SSGSGEELAR LAGVPFLGSV PLDSQLTRSL EEGRDFIQEF
260 270
PKSTAYSALT SIAQRVVHRM SALCS
Length:275
Mass (Da):29,518
Last modified:May 1, 2000 - v1
Checksum:iFE0C23BE4691B4CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114169 mRNA. Translation: AAF01785.1.
AK003816 mRNA. Translation: BAB23013.1.
BC012635 mRNA. Translation: AAH12635.1.
CCDSiCCDS28500.1.
RefSeqiNP_036086.1. NM_011956.3.
UniGeneiMm.36718.

Genome annotation databases

EnsembliENSMUST00000044252; ENSMUSP00000049319; ENSMUSG00000039183.
GeneIDi26426.
KEGGimmu:26426.
UCSCiuc008ayo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114169 mRNA. Translation: AAF01785.1.
AK003816 mRNA. Translation: BAB23013.1.
BC012635 mRNA. Translation: AAH12635.1.
CCDSiCCDS28500.1.
RefSeqiNP_036086.1. NM_011956.3.
UniGeneiMm.36718.

3D structure databases

ProteinModelPortaliQ9R061.
SMRiQ9R061. Positions 21-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049319.

PTM databases

PhosphoSiteiQ9R061.

Proteomic databases

EPDiQ9R061.
MaxQBiQ9R061.
PaxDbiQ9R061.
PeptideAtlasiQ9R061.
PRIDEiQ9R061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044252; ENSMUSP00000049319; ENSMUSG00000039183.
GeneIDi26426.
KEGGimmu:26426.
UCSCiuc008ayo.2. mouse.

Organism-specific databases

CTDi10101.
MGIiMGI:1347072. Nubp2.

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00550000075057.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ9R061.
OMAiSHLGADP.
OrthoDBiEOG091G0DY5.
PhylomeDBiQ9R061.
TreeFamiTF354321.

Miscellaneous databases

PROiQ9R061.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039183.
CleanExiMM_NUBP2.
GenevisibleiQ9R061. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03039. NUBP2. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUBP2_MOUSE
AccessioniPrimary (citable) accession number: Q9R061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.