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Protein

Cytosolic Fe-S cluster assembly factor NUBP2

Gene

Nubp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure (PubMed:23807208).UniRule annotation1 Publication

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi200Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi203Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP2UniRule annotation
Alternative name(s):
Nucleotide-binding protein 2UniRule annotation
Short name:
NBP 2UniRule annotation
Gene namesi
Name:Nubp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1347072 Nubp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001849461 – 275Cytosolic Fe-S cluster assembly factor NUBP2Add BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineUniRule annotationBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9R061
MaxQBiQ9R061
PaxDbiQ9R061
PeptideAtlasiQ9R061
PRIDEiQ9R061

PTM databases

PhosphoSitePlusiQ9R061

Expressioni

Tissue specificityi

Widely expressed.

Developmental stagei

Expressed at 7, 11, 15 and 17 dpc.

Gene expression databases

BgeeiENSMUSG00000039183
CleanExiMM_NUBP2
GenevisibleiQ9R061 MM

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (By similarity). Interacts with KIFC1 (PubMed:16638812). Interacts with NUBP1 (PubMed:16638812, PubMed:23807208).UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi204978, 1 interactor
STRINGi10090.ENSMUSP00000049319

Structurei

3D structure databases

ProteinModelPortaliQ9R061
SMRiQ9R061
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ Eukaryota
COG0489 LUCA
GeneTreeiENSGT00550000075057
HOGENOMiHOG000079916
HOVERGENiHBG051027
InParanoidiQ9R061
OMAiRELTFCK
OrthoDBiEOG091G0DY5
PhylomeDBiQ9R061
TreeFamiTF354321

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03039 NUBP2, 1 hit
InterProiView protein in InterPro
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028600 NUBP2/Cfd1_eukaryotes
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9R061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAAAGERAE PGNLAGVRHI ILVLSGKGGV GKSTISTELA LALRHQGKKV
60 70 80 90 100
GILDVDLCGP SIPHMLRAQG KAVHQCDNGW VPVFVDQEQS ISLMSVGFLL
110 120 130 140 150
ENPDEAVVWR GPKKHALIKQ FVSDVAWGQL DYLVVDTPPG TSDEHMATME
160 170 180 190 200
ALRPYRPLGA LVVTTPQAVS IGDVRRELTF CKKTGLQVIG VIENMSGFTC
210 220 230 240 250
PHCAECTNVF SSGSGEELAR LAGVPFLGSV PLDSQLTRSL EEGRDFIQEF
260 270
PKSTAYSALT SIAQRVVHRM SALCS
Length:275
Mass (Da):29,518
Last modified:May 1, 2000 - v1
Checksum:iFE0C23BE4691B4CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114169 mRNA Translation: AAF01785.1
AK003816 mRNA Translation: BAB23013.1
BC012635 mRNA Translation: AAH12635.1
CCDSiCCDS28500.1
RefSeqiNP_036086.1, NM_011956.3
UniGeneiMm.36718

Genome annotation databases

EnsembliENSMUST00000044252; ENSMUSP00000049319; ENSMUSG00000039183
GeneIDi26426
KEGGimmu:26426
UCSCiuc008ayo.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNUBP2_MOUSE
AccessioniPrimary (citable) accession number: Q9R061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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