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Protein

Cytosolic Fe-S cluster assembly factor NUBP1

Gene

Nubp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (By similarity). Implicated in the regulation of centrosome duplication (PubMed:16638812, PubMed:23807208). Negatively regulates cilium formation and structure (PubMed:23807208).UniRule annotation2 Publications

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi22 – 221Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi25 – 251Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi31 – 311Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi235 – 2351Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi238 – 2381Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 698ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell growth Source: MGI
  • cell projection organization Source: UniProtKB-KW
  • cellular iron ion homeostasis Source: MGI
  • centrosome localization Source: MGI
  • iron-sulfur cluster assembly Source: UniProtKB
  • negative regulation of centrosome duplication Source: MGI
  • protein localization to cell cortex Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1UniRule annotation
Alternative name(s):
Nucleotide-binding protein 1UniRule annotation
Short name:
NBP 1UniRule annotation
Gene namesi
Name:Nubp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1347073. Nubp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Cytosolic Fe-S cluster assembly factor NUBP1PRO_0000184944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei319 – 3191PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9R060.
MaxQBiQ9R060.
PaxDbiQ9R060.
PRIDEiQ9R060.

PTM databases

PhosphoSiteiQ9R060.

Expressioni

Tissue specificityi

Expressed in trachea epithelial cells, and kidney inner medullary collecting duct cells.1 Publication

Inductioni

High and constant expression in cycling cells. Down-regulated upon cell cycle exit and quiescence.1 Publication

Gene expression databases

BgeeiQ9R060.
CleanExiMM_NUBP1.
GenevisibleiQ9R060. MM.

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (PubMed:16638812). Interacts with KIFC1 (PubMed:16638812). Interacts with NUBP2 (PubMed:16638812). Interacts with the BBS/CCT complex subunit CCT1 (PubMed:23807208).UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023146.

Structurei

3D structure databases

ProteinModelPortaliQ9R060.
SMRiQ9R060. Positions 44-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00390000009735.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ9R060.
OMAiMCAEMGI.
OrthoDBiEOG7D59NZ.
PhylomeDBiQ9R060.
TreeFamiTF300755.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03038. NUBP1.
InterProiIPR003593. AAA+_ATPase.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEAPHGCPG ADSAQAGRGA SCQGCPNQRL CASGAGAAPD PAVEEIREKM
60 70 80 90 100
KTVRHKLLVL SGKGGVGKST FSAHLAHGLA EDGDTQVALL DIDICGPSIP
110 120 130 140 150
KIMGLEGEQV HQSGSGWSPV YVDDNLGVMS VGFLLSSPDD AVIWRGPKKN
160 170 180 190 200
GMIKQFLRDV DWGDVDYLIV DTPPGTSDEH LSVVQYLAAA HIDGAVILTT
210 220 230 240 250
PQEVALQDVR KEISFCHKVK LPIIGVVENM SGFICPKCKK ESQIFPPTTG
260 270 280 290 300
GAEAMCQDLR IPLLGKVPLD PHIGKSCDKG QSFFVEAPDS PATAAYRSII
310 320
QRIRDFCNSH QSHAETLISP
Length:320
Mass (Da):34,085
Last modified:May 1, 2000 - v1
Checksum:iAB56BDF7D730F311
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701V → I in AAH55436 (PubMed:15489334).Curated
Sequence conflicti235 – 2351C → S in BAB32007 (PubMed:16141072).Curated
Sequence conflicti260 – 2601R → K in AAH55436 (PubMed:15489334).Curated
Sequence conflicti310 – 3101H → R in AAH55436 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114170 mRNA. Translation: AAF01786.1.
AK020141 mRNA. Translation: BAB32007.1.
AK157212 mRNA. Translation: BAE34002.1.
BC055436 mRNA. Translation: AAH55436.1.
CCDSiCCDS27948.1.
RefSeqiNP_036085.1. NM_011955.2.
UniGeneiMm.29037.
Mm.325732.

Genome annotation databases

EnsembliENSMUST00000023146; ENSMUSP00000023146; ENSMUSG00000022503.
GeneIDi26425.
KEGGimmu:26425.
UCSCiuc007ydo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114170 mRNA. Translation: AAF01786.1.
AK020141 mRNA. Translation: BAB32007.1.
AK157212 mRNA. Translation: BAE34002.1.
BC055436 mRNA. Translation: AAH55436.1.
CCDSiCCDS27948.1.
RefSeqiNP_036085.1. NM_011955.2.
UniGeneiMm.29037.
Mm.325732.

3D structure databases

ProteinModelPortaliQ9R060.
SMRiQ9R060. Positions 44-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023146.

PTM databases

PhosphoSiteiQ9R060.

Proteomic databases

EPDiQ9R060.
MaxQBiQ9R060.
PaxDbiQ9R060.
PRIDEiQ9R060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023146; ENSMUSP00000023146; ENSMUSG00000022503.
GeneIDi26425.
KEGGimmu:26425.
UCSCiuc007ydo.2. mouse.

Organism-specific databases

CTDi4682.
MGIiMGI:1347073. Nubp1.

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00390000009735.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ9R060.
OMAiMCAEMGI.
OrthoDBiEOG7D59NZ.
PhylomeDBiQ9R060.
TreeFamiTF300755.

Miscellaneous databases

ChiTaRSiNubp1. mouse.
NextBioi304457.
PROiQ9R060.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R060.
CleanExiMM_NUBP1.
GenevisibleiQ9R060. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03038. NUBP1.
InterProiIPR003593. AAA+_ATPase.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two novel mouse genes -- Nubp2, mapped to the T-complex on chromosome 17, and Nubp1, mapped to chromosome 16 -- establish a new gene family of nucleotide-binding proteins in eukaryotes."
    Nakashima H., Grahovac M.J., Mazzarella R., Fujiwara H., Kitchen J.R., Threat T.A., Ko M.S.H.
    Genomics 60:152-160(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Retina, Spleen and Wolffian duct.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication."
    Christodoulou A., Lederer C.W., Surrey T., Vernos I., Santama N.
    J. Cell Sci. 119:2035-2047(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KIFC1 AND NUBP2.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. Cited for: FUNCTION, INTERACTION WITH CCT1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNUBP1_MOUSE
AccessioniPrimary (citable) accession number: Q9R060
Secondary accession number(s): Q3U053, Q7TMI1, Q9CRM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: March 16, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

NIH3T3 cells expressing reduced levels of Nubp1 show an increase in number of centrosomes per cell and an increase in the fraction of multinucleated cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.