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Protein

Cytosolic Fe-S cluster assembly factor NUBP1

Gene

Nubp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (By similarity). Implicated in the regulation of centrosome duplication (PubMed:16638812, PubMed:23807208). Negatively regulates cilium formation and structure (PubMed:23807208).UniRule annotation2 Publications

Miscellaneous

NIH3T3 cells expressing reduced levels of Nubp1 show an increase in number of centrosomes per cell and an increase in the fraction of multinucleated cells.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi22Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi25Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi235Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi238Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 69ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cell projection organization Source: UniProtKB-KW
  • cellular iron ion homeostasis Source: MGI
  • centrosome localization Source: MGI
  • iron-sulfur cluster assembly Source: UniProtKB
  • negative regulation of centrosome duplication Source: MGI
  • protein localization to cell cortex Source: MGI

Keywordsi

Biological processCilium biogenesis/degradation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1UniRule annotation
Alternative name(s):
Nucleotide-binding protein 1UniRule annotation
Short name:
NBP 1UniRule annotation
Gene namesi
Name:Nubp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1347073 Nubp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001849441 – 320Cytosolic Fe-S cluster assembly factor NUBP1Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei319PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9R060
MaxQBiQ9R060
PaxDbiQ9R060
PeptideAtlasiQ9R060
PRIDEiQ9R060

PTM databases

PhosphoSitePlusiQ9R060

Expressioni

Tissue specificityi

Expressed in trachea epithelial cells, and kidney inner medullary collecting duct cells.1 Publication

Inductioni

High and constant expression in cycling cells. Down-regulated upon cell cycle exit and quiescence.1 Publication

Gene expression databases

BgeeiENSMUSG00000022503
CleanExiMM_NUBP1
GenevisibleiQ9R060 MM

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (PubMed:16638812). Interacts with KIFC1 (PubMed:16638812). Interacts with NUBP2 (PubMed:16638812). Interacts with the BBS/CCT complex subunit CCT1 (PubMed:23807208).UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023146

Structurei

3D structure databases

ProteinModelPortaliQ9R060
SMRiQ9R060
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ Eukaryota
COG0489 LUCA
GeneTreeiENSGT00390000009735
HOGENOMiHOG000079916
HOVERGENiHBG051027
InParanoidiQ9R060
OMAiCPNQAIC
OrthoDBiEOG091G0DY5
PhylomeDBiQ9R060
TreeFamiTF300755

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03038 NUBP1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028601 NUBP1/Nbp35
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9R060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEAPHGCPG ADSAQAGRGA SCQGCPNQRL CASGAGAAPD PAVEEIREKM
60 70 80 90 100
KTVRHKLLVL SGKGGVGKST FSAHLAHGLA EDGDTQVALL DIDICGPSIP
110 120 130 140 150
KIMGLEGEQV HQSGSGWSPV YVDDNLGVMS VGFLLSSPDD AVIWRGPKKN
160 170 180 190 200
GMIKQFLRDV DWGDVDYLIV DTPPGTSDEH LSVVQYLAAA HIDGAVILTT
210 220 230 240 250
PQEVALQDVR KEISFCHKVK LPIIGVVENM SGFICPKCKK ESQIFPPTTG
260 270 280 290 300
GAEAMCQDLR IPLLGKVPLD PHIGKSCDKG QSFFVEAPDS PATAAYRSII
310 320
QRIRDFCNSH QSHAETLISP
Length:320
Mass (Da):34,085
Last modified:May 1, 2000 - v1
Checksum:iAB56BDF7D730F311
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170V → I in AAH55436 (PubMed:15489334).Curated1
Sequence conflicti235C → S in BAB32007 (PubMed:16141072).Curated1
Sequence conflicti260R → K in AAH55436 (PubMed:15489334).Curated1
Sequence conflicti310H → R in AAH55436 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114170 mRNA Translation: AAF01786.1
AK020141 mRNA Translation: BAB32007.1
AK157212 mRNA Translation: BAE34002.1
BC055436 mRNA Translation: AAH55436.1
CCDSiCCDS27948.1
RefSeqiNP_036085.1, NM_011955.2
UniGeneiMm.29037
Mm.325732

Genome annotation databases

EnsembliENSMUST00000023146; ENSMUSP00000023146; ENSMUSG00000022503
GeneIDi26425
KEGGimmu:26425
UCSCiuc007ydo.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNUBP1_MOUSE
AccessioniPrimary (citable) accession number: Q9R060
Secondary accession number(s): Q3U053, Q7TMI1, Q9CRM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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