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Protein

Four and a half LIM domains protein 3

Gene

Fhl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. actin binding Source: MGI
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Four and a half LIM domains protein 3
Short name:
FHL-3
Alternative name(s):
Skeletal muscle LIM-protein 2
Short name:
SLIM-2
Gene namesi
Name:Fhl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1341092. Fhl3.

Subcellular locationi

GO - Cellular componenti

  1. focal adhesion Source: MGI
  2. nucleus Source: MGI
  3. stress fiber Source: MGI
  4. Z disc Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 289288Four and a half LIM domains protein 3PRO_0000075741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei157 – 1571N6-acetyllysine1 Publication
Modified residuei244 – 2441N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9R059.
PaxDbiQ9R059.
PRIDEiQ9R059.

PTM databases

PhosphoSiteiQ9R059.

Expressioni

Tissue specificityi

In the adult, expressed at high levels in skeletal muscle and, to a lesser extent, in heart, lung, skin and kidney. During embryonic development, expressed ubiquitously at low levels.2 Publications

Gene expression databases

CleanExiMM_FHL3.
ExpressionAtlasiQ9R059. baseline and differential.
GenevestigatoriQ9R059.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Sox15P432677EBI-7332617,EBI-7332587

Protein-protein interaction databases

IntActiQ9R059. 1 interaction.
MINTiMINT-4788495.

Structurei

3D structure databases

ProteinModelPortaliQ9R059.
SMRiQ9R059. Positions 1-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 9253LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini101 – 15353LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini162 – 21251LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST
Domaini221 – 27555LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

LIM domain, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiQ9R059.
OMAiYEDRHYH.
PhylomeDBiQ9R059.
TreeFamiTF314113.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 3 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAFDCAKC NESLYGRKYI QTDSGPYCVP CYDNTFANTC AECQQLIGHD
60 70 80 90 100
SRELFYEDRH FHEGCFRCCR CQRSLADEPF TCQDSELLCN ECYCTAFSSQ
110 120 130 140 150
CSACGETVMP GSRKLEYGGQ TWHEHCFLCS GCEQPLGSRS FVPDKGAHYC
160 170 180 190 200
VPCYENKFAP RCARCSKTLT QGGVTYRDQP WHRECLVCTG CKTPLAGQQF
210 220 230 240 250
TSRDDDPYCV ACFGELFAPK CSSCKRPITG GSGGGEGAGL GGGKYVSFED
260 270 280
RHWHHSCFSC ARCSTSLVGQ GFVPDGDQVL CQGCSQAGP
Length:289
Mass (Da):31,795
Last modified:October 3, 2012 - v2
Checksum:i38168919F5303237
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771D → G in AAD32623 (PubMed:10049693).Curated
Sequence conflicti91 – 911E → D in AAD32623 (PubMed:10049693).Curated
Sequence conflicti95 – 951T → S in AAD32623 (PubMed:10049693).Curated
Sequence conflicti137 – 1371G → A in AAD53231 (PubMed:10906474).Curated
Sequence conflicti157 – 1593KFA → NLT in AAF73159 (Ref. 5) Curated
Sequence conflicti192 – 1921K → Q in AAD32623 (PubMed:10049693).Curated
Sequence conflicti199 – 1991Q → H in AAD53231 (PubMed:10906474).Curated
Sequence conflicti205 – 2051D → E in AAD32623 (PubMed:10049693).Curated
Sequence conflicti225 – 2251K → N in AAD53231 (PubMed:10906474).Curated
Sequence conflicti229 – 23810TGGSGGGEGA → V in AAD32623 (PubMed:10049693).Curated
Sequence conflicti235 – 2351G → A in AAD53231 (PubMed:10906474).Curated
Sequence conflicti256 – 2561S → N in AAD32623 (PubMed:10049693).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134772 mRNA. Translation: AAD32623.1.
AF114382 mRNA. Translation: AAD53231.1.
AL606907 Genomic DNA. No translation available.
BC144903 mRNA. Translation: AAI44904.1.
BC145939 mRNA. Translation: AAI45940.1.
AF149826 mRNA. Translation: AAF73159.1.
CCDSiCCDS38874.1.
RefSeqiNP_034343.2. NM_010213.3.
XP_006502831.1. XM_006502768.2.
UniGeneiMm.331856.

Genome annotation databases

EnsembliENSMUST00000038684; ENSMUSP00000040150; ENSMUSG00000032643.
GeneIDi14201.
KEGGimmu:14201.
UCSCiuc008uqv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134772 mRNA. Translation: AAD32623.1.
AF114382 mRNA. Translation: AAD53231.1.
AL606907 Genomic DNA. No translation available.
BC144903 mRNA. Translation: AAI44904.1.
BC145939 mRNA. Translation: AAI45940.1.
AF149826 mRNA. Translation: AAF73159.1.
CCDSiCCDS38874.1.
RefSeqiNP_034343.2. NM_010213.3.
XP_006502831.1. XM_006502768.2.
UniGeneiMm.331856.

3D structure databases

ProteinModelPortaliQ9R059.
SMRiQ9R059. Positions 1-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R059. 1 interaction.
MINTiMINT-4788495.

PTM databases

PhosphoSiteiQ9R059.

Proteomic databases

MaxQBiQ9R059.
PaxDbiQ9R059.
PRIDEiQ9R059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038684; ENSMUSP00000040150; ENSMUSG00000032643.
GeneIDi14201.
KEGGimmu:14201.
UCSCiuc008uqv.2. mouse.

Organism-specific databases

CTDi2275.
MGIiMGI:1341092. Fhl3.

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiQ9R059.
OMAiYEDRHYH.
PhylomeDBiQ9R059.
TreeFamiTF314113.

Miscellaneous databases

NextBioi13895005.
PROiQ9R059.
SOURCEiSearch...

Gene expression databases

CleanExiMM_FHL3.
ExpressionAtlasiQ9R059. baseline and differential.
GenevestigatoriQ9R059.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 3 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle."
    Morgan M.J., Madgwick A.J.A.
    Biochem. Biophys. Res. Commun. 255:245-250(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system."
    Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J.
    Mech. Dev. 95:259-265(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "The cloning, sequencing and characterization of a mouse FHL3, which contains four and a half LIM domains."
    Li H.Y., Lee S.M.Y., Tsui S.K.W., Chan K.K., Kotaka M., Chim S.S.C., Lee C.Y., Fung K.P., Waye M.M.Y.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-228.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-157 AND LYS-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiFHL3_MOUSE
AccessioniPrimary (citable) accession number: Q9R059
Secondary accession number(s): A6H6N4, Q9JLP5, Q9WUH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: October 3, 2012
Last modified: April 1, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.