Q9R059 (FHL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Four and a half LIM domains protein 3
Short name=FHL-3

Alternative name(s):
Skeletal muscle LIM-protein 2
Short name=SLIM-2

Gene names

Name:

Fhl3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Tissue specificity	In the adult, expressed at high levels in skeletal muscle and, to a lesser extent, in heart, lung, skin and kidney. During embryonic development, expressed ubiquitously at low levels. Ref.1 Ref.2
Sequence similarities	Contains 4 LIM zinc-binding domains.

Ontologies

Keywords
Domain	LIM domain Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from direct assay PubMed 12704194. Source: MGI
Cellular_component	Z disc Inferred from direct assay PubMed 12704194. Source: MGI nucleus Inferred from direct assay PubMed 12704194. Source: MGI stress fiber Inferred from direct assay PubMed 12704194. Source: MGI
Molecular_function	actin binding Inferred from direct assay PubMed 12704194. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Four and a half LIM domains protein 3		PRO_0000075741
Regions
	Domain	40 – 92	53	LIM zinc-binding 1
	Domain	101 – 153	53	LIM zinc-binding 2
	Domain	162 – 212	51	LIM zinc-binding 3
	Domain	221 – 275	55	LIM zinc-binding 4
	Zinc finger	7 – 31	25	C4-type Potential
Experimental info
	Sequence conflict	77	1	D → G in AAD32623. Ref.1
	Sequence conflict	91	1	E → D in AAD32623. Ref.1
	Sequence conflict	95	1	T → S in AAD32623. Ref.1
	Sequence conflict	137	1	G → A in AAD53231. Ref.2
	Sequence conflict	157 – 159	3	KFA → NLT in AAF73159. Ref.5
	Sequence conflict	192	1	K → Q in AAD32623. Ref.1
	Sequence conflict	199	1	Q → H in AAD53231. Ref.2
	Sequence conflict	205	1	D → E in AAD32623. Ref.1
	Sequence conflict	225	1	K → N in AAD53231. Ref.2
	Sequence conflict	229 – 238	10	TGGSGGGEGA → V in AAD32623. Ref.1
	Sequence conflict	235	1	G → A in AAD53231. Ref.2
	Sequence conflict	256	1	S → N in AAD32623. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9R059 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 38168919F5303237	FASTA	289	31,795
	10 20 30 40 50 60 MSEAFDCAKC NESLYGRKYI QTDSGPYCVP CYDNTFANTC AECQQLIGHD SRELFYEDRH 70 80 90 100 110 120 FHEGCFRCCR CQRSLADEPF TCQDSELLCN ECYCTAFSSQ CSACGETVMP GSRKLEYGGQ 130 140 150 160 170 180 TWHEHCFLCS GCEQPLGSRS FVPDKGAHYC VPCYENKFAP RCARCSKTLT QGGVTYRDQP 190 200 210 220 230 240 WHRECLVCTG CKTPLAGQQF TSRDDDPYCV ACFGELFAPK CSSCKRPITG GSGGGEGAGL 250 260 270 280 GGGKYVSFED RHWHHSCFSC ARCSTSLVGQ GFVPDGDQVL CQGCSQAGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle." Morgan M.J., Madgwick A.J.A. Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system." Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J. Mech. Dev. 95:259-265(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The cloning, sequencing and characterization of a mouse FHL3, which contains four and a half LIM domains." Li H.Y., Lee S.M.Y., Tsui S.K.W., Chan K.K., Kotaka M., Chim S.S.C., Lee C.Y., Fung K.P., Waye M.M.Y. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF134772 mRNA. Translation: AAD32623.1. AF114382 mRNA. Translation: AAD53231.1. AL606907 Genomic DNA. No translation available. BC144903 mRNA. Translation: AAI44904.1. BC145939 mRNA. Translation: AAI45940.1. AF149826 mRNA. Translation: AAF73159.1.
IPI	IPI00345772. IPI00828338.
RefSeq	NP_034343.2. NM_010213.3.
UniGene	Mm.331856.
3D structure databases
ProteinModelPortal	Q9R059.
SMR	Q9R059. Positions 30-286.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4788495.
PTM databases
PhosphoSite	Q9R059.
Proteomic databases
PaxDb	Q9R059.
PRIDE	Q9R059.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038684; ENSMUSP00000040150; ENSMUSG00000032643.
GeneID	14201.
KEGG	mmu:14201.
Organism-specific databases
CTD	2275.
MGI	MGI:1341092. Fhl3.
Phylogenomic databases
eggNOG	NOG314122.
GeneTree	ENSGT00690000101670.
HOGENOM	HOG000231075.
HOVERGEN	HBG074526.
InParanoid	Q9R059.
OMA	QFTSRDD.
Gene expression databases
CleanEx	MM_FHL3.
Genevestigator	Q9R059.
GermOnline	ENSMUSG00000032643. Mus musculus.
Family and domain databases
Gene3D	2.10.110.10. 5 hits.
InterPro	IPR001781. Znf_LIM. [Graphical view]
Pfam	PF00412. LIM. 4 hits. [Graphical view]
SMART	SM00132. LIM. 4 hits. [Graphical view]
PROSITE	PS00478. LIM_DOMAIN_1. 3 hits. PS50023. LIM_DOMAIN_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	13895005.
SOURCE	Search...

Entry information

Entry name

FHL3_MOUSE

Accession

Primary (citable) accession number: Q9R059
Secondary accession number(s): A6H6N4, Q9JLP5, Q9WUH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program