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Reviewed, UniProtKB/Swiss-Prot Q9R049 (AMFR_MOUSE)

Last modified January 19, 2010. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Autocrine motility factor receptor
      Short name=AMF receptor
    EC=6.3.2.19
Gene names
Name: Amfr
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length643 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Specific receptor for the autocrine motility factor. Possesses ubiquitin ligase activity and can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. Ref.3 UniProtKB P26442 UniProtKB Q9UKV5

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. UniProtKB Q9UKV5

Subunit structure

Interacts with UBE2G2/UBC7 through a region distinct from the RING finger. Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Ref.4 UniProtKB Q9UKV5

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in heart, brain, liver, lung, skeletal muscle, kidney and testis. Not detected in spleen. Ref.1

Sequence similarities

Contains 1 CUE domain.

Contains 1 RING-type zinc finger.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q9R049-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9R049-2)

The sequence of this isoform differs from the canonical sequence as follows:
     57-60: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 643643Autocrine motility factor receptor
PRO_0000064580

Regions

Transmembrane82 – 10221 Potential
Transmembrane122 – 14221 Potential
Transmembrane186 – 20621 Potential
Transmembrane215 – 23521 Potential
Transmembrane254 – 27421 Potential
Transmembrane276 – 29621 Potential
Transmembrane429 – 44921 Potential
Domain456 – 49843CUE
Zinc finger341 – 37939RING-type

Amino acid modifications

Modified residue5421Phosphoserine Ref.5 Ref.6

Natural variations

Alternative sequence57 – 604Missing in isoform 2.
VSP_008224

Experimental info

Sequence conflict4121R → S in AAD56721. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 2.
Checksum: 7119277725982F79

FASTA64373,105
        10         20         30         40         50         60 
MPLLFLERFP WPSLRTYTGL SGLALLGTIV SAYRALSQPE DGSGEPEPLT APLQPEALAP 

        70         80         90        100        110        120 
ARLTAGGPRA RDVAQYLLSD SLFVWVLVNT ACCVLMLVAK LIQCIVFGPL RVSERQHLKD 

       130        140        150        160        170        180 
KFWNFIFYKF IFIFGVLNVQ TVEEVVMWCL WFAGLVFLHL MVQLCKDRFE YLSFSPTTPM 

       190        200        210        220        230        240 
SSHGRVLSLL IAMLLSCCGL AVVCCVTGYT HGMHTLAFMA AESLLVTVRT AHVILRYVIH 

       250        260        270        280        290        300 
LWDLNHEGTW EGKGTYVYYT DFVMELALLS LDLMHHIHML LFGNIWLSMA SLVIFMQLRY 

       310        320        330        340        350        360 
LFHEVQRRIR RHKNYLRVVG NMEARFAVAT PEELAVNNDD CAICWDSMQA ARKLPCGHLF 

       370        380        390        400        410        420 
HNSCLRSWLE QDTSCPTCRM SLNIADGSRA REDHQGENLD ENLVPVAAAE GRPRLNQHNH 

       430        440        450        460        470        480 
FFHFDGSRIA SWLPSFSVEV MHTTNILGIT QASNSQLNAM AHQIQEMFPQ VPYHLVLQDL 

       490        500        510        520        530        540 
QMTRSVEITT DNILEGRIQV PFPTQRSDSL RPALNSPVER PSPDLEEGEA SVQTERVPLD 

       550        560        570        580        590        600 
LSPRLEETLD FSEVELEPIE VEDFEARGSR FSKSADERQR MLVQRKDDLL QQARKRFLNK 

       610        620        630        640 
SSEDDGASER LLPSEGTSSD PVTLRRRMLA AAAERRLQRQ RTT 

« Hide

Isoform 2.

Checksum: 22FF6303EEA033F1
Show »

FASTA63972,753

References

« Hide 'large scale' references
[1]"The autocrine motility factor receptor gene encodes a novel type of seven transmembrane protein."
Shimizu K., Tani M., Watanabe H., Nagamachi Y., Niinaka Y., Shiroishi T., Ohwada S., Raz A., Yokota J.
FEBS Lett. 456:295-300(1999) [PubMed: 10456327] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Lung and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: FVB/N.
Tissue: Colon and Mammary gland.
[3]"Overexpression of autocrine motility factor receptor (AMFR) in NIH3T3 fibroblasts induces cell transformation."
Onishi Y., Tsukada K., Yokota J., Raz A.
Clin. Exp. Metastasis 20:51-58(2003) [PubMed: 12650607] [Abstract]
Cited for: ROLE IN METASTASIS.
[4]"The AAA ATPase p97 links peptide N-glycanase to the endoplasmic reticulum-associated E3 ligase autocrine motility factor receptor."
Li G., Zhao G., Zhou X., Schindelin H., Lennarz W.J.
Proc. Natl. Acad. Sci. U.S.A. 103:8348-8353(2006) [PubMed: 16709668] [Abstract]
Cited for: INTERACTION WITH NGLY1; PSMC1; SAKS1; RAD23B AND VCP.
[5]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-542, MASS SPECTROMETRY.
Tissue: Brain cortex.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-542, MASS SPECTROMETRY.
Tissue: Macrophage.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF124144 mRNA. Translation: AAD56721.1.
BC003256 mRNA. Translation: AAH03256.1.
BC034538 mRNA. Translation: AAH34538.1.
BC040338 mRNA. Translation: AAH40338.1.
IPIIPI00319880.
IPI00351315.
RefSeqNP_035917.2.
UniGeneMm.34641

3D structure databases

SMRQ9R049. Positions 330-386, 456-502.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9R049.

PTM databases

PhosphoSiteQ9R049.

Proteomic databases

PRIDEQ9R049.

Genome annotation databases

EnsemblENSMUST00000053766; ENSMUSP00000052258; ENSMUSG00000031751; Mus musculus. [Genome view]
GeneID23802.
KEGGmmu:23802.
UCSCuc009mvn.1. mouse.

Organism-specific databases

CTD23802.
MGIMGI:1345634. Amfr.

Phylogenomic databases

HOGENOMHBG445825.
HOVERGENQ9R049.
InParanoidQ9R049.
OMAARDVAQY.

Enzyme and pathway databases

BRENDA6.3.2.19. 244.

Gene expression databases

ArrayExpressQ9R049.
BgeeQ9R049.
CleanExMM_AMFR.
GenevestigatorQ9R049.
GermOnlineENSMUSG00000031751. Mus musculus.

Family and domain databases

InterProIPR003892. CUE.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
[Graphical view]
PfamPF02845. CUE. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTSM00546. CUE. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEPS51140. CUE. 1 hit.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameAMFR_MOUSE
AccessionPrimary (citable) accession number: Q9R049
Secondary accession number(s): Q8K008, Q99LH5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 1, 2003
Last modified: January 19, 2010
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents