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Protein

Serine/threonine-protein kinase PLK3

Gene

Plk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91ATPPROSITE-ProRule annotation1
Active sitei185Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 76ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • p53 binding Source: UniProtKB
  • protein kinase activity Source: RGD
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle, DNA damage
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain
R-RNO-6804756 Regulation of TP53 Activity through Phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PLK3 (EC:2.7.11.21)
Alternative name(s):
Cytokine-inducible serine/threonine-protein kinase
FGF-inducible kinase
Polo-like kinase 3
Short name:
PLK-3
Gene namesi
Name:Plk3
Synonyms:Cnk, Fnk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi62039 Plk3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004147091 – 647Serine/threonine-protein kinase PLK3Add BLAST647

Post-translational modificationi

Phosphorylated in an ATM-dependent manner following DNA damage. Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9R011
PRIDEiQ9R011

PTM databases

PhosphoSitePlusiQ9R011

Expressioni

Tissue specificityi

Constitutively expressed in post-mitotic neurons.1 Publication

Inductioni

By the intense activity associated with seizures.1 Publication

Gene expression databases

BgeeiENSRNOG00000018484
GenevisibleiQ9R011 RN

Interactioni

Subunit structurei

Interacts with GOLGB1 (By similarity). Interacts (via the POLO-box domain) with CIB1; leading to inhibit PLK3 kinase activity.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248673, 1 interactor
STRINGi10116.ENSRNOP00000025197

Structurei

3D structure databases

ProteinModelPortaliQ9R011
SMRiQ9R011
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 314Protein kinasePROSITE-ProRule annotationAdd BLAST253
Domaini471 – 534POLO box 1PROSITE-ProRule annotationAdd BLAST64
Domaini568 – 638POLO box 2PROSITE-ProRule annotationAdd BLAST71

Domaini

The POLO box domains act as phosphopeptide-binding module that recognize and bind serine-[phosphothreonine/phosphoserine]-(proline/X) motifs. PLK3 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them. The POLO box domains mediates localization to the centrosome (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0575 Eukaryota
ENOG410XQBP LUCA
GeneTreeiENSGT00530000062954
HOGENOMiHOG000248546
HOVERGENiHBG001843
InParanoidiQ9R011
KOiK08862
OMAiLCALRNC
OrthoDBiEOG091G0D89
TreeFamiTF101089

Family and domain databases

CDDicd13118 POLO_box_1, 1 hit
cd13117 POLO_box_2, 1 hit
Gene3Di3.30.1120.30, 3 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR033701 POLO_box_1
IPR033695 POLO_box_2
IPR000959 POLO_box_dom
IPR036947 POLO_box_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR020658 Ser/Thr_kinase_Plk3
PANTHERiPTHR24345:SF42 PTHR24345:SF42, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00659 POLO_box, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50078 POLO_BOX, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9R011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPAAGFLSP RPFPRAAAPS SPPAGPGPPA SASPRSEPGV LAGPQTPDAS
60 70 80 90 100
RLITDPRSGR TYIKGRLLGK GGFARCYEAT DTETSIAYAV KVIPQSRVAK
110 120 130 140 150
PHQREKIINE IELHRDLQHR HIVRFSHHFE DADNIYIFLE LCSRKSLAHI
160 170 180 190 200
WKARHTLLEP EVRYYLRQIL SGLKYLHQRG ILHRDLKLGN FFITDNMELK
210 220 230 240 250
VGDFGLAARL EPPEQRKKTI CGTPNYVAPE VLLRQGHGPE ADVWSLGCVM
260 270 280 290 300
YTLLCGSPPF ETADLKETYR CIKQVHYTLP ASLSLPARQL LAAILRASPR
310 320 330 340 350
DRPSIEQILR HDFFTKGYTP DRLPVSSCVT VPDLTPPNPA RSLFAKVTKS
360 370 380 390 400
LFGRRKSKNK NHSEEQDNVS CLVSGLMRTS IGHPDVRPEA PAASALAPVS
410 420 430 440 450
LVETAAEDSS PRGTLASSGD GFEEGLTVTT VVESALCALR NCVAFMPPAE
460 470 480 490 500
QNPAPLAQPE PLVWVSKWVD YSNKFGFGYQ LSSRRVAVLF NDGTHMALSA
510 520 530 540 550
NRKTVHYNPT STKHFSFSVG SVPRALQPQL GILRYFASYM EQHLMKGGDL
560 570 580 590 600
PSVEEVEVPA PPLLLQWVKT DQALLMLFSD GTVQVNFYGD HTKLILSGWE
610 620 630 640
PLLVTFVARN RSACTYLASH LRQLGCSPDL RQRLRYALRL LRDRSPA
Length:647
Mass (Da):71,768
Last modified:December 14, 2011 - v2
Checksum:i44FAFD446E5F9999
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136584 mRNA Translation: AAF08367.1
RefSeqiNP_071523.1, NM_022187.1
UniGeneiRn.9284

Genome annotation databases

EnsembliENSRNOT00000025197; ENSRNOP00000025197; ENSRNOG00000018484
GeneIDi58936
KEGGirno:58936
UCSCiRGD:62039 rat

Similar proteinsi

Entry informationi

Entry nameiPLK3_RAT
AccessioniPrimary (citable) accession number: Q9R011
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: December 14, 2011
Last modified: May 23, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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