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Protein

Integrin beta-1-binding protein 2

Gene

Itgb1bp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role during maturation and/or organization of muscles cells.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5Zinc 1PROSITE-ProRule annotation1
Metal bindingi10Zinc 1PROSITE-ProRule annotation1
Metal bindingi24Zinc 1PROSITE-ProRule annotation1
Metal bindingi27Zinc 2PROSITE-ProRule annotation1
Metal bindingi42Zinc 2PROSITE-ProRule annotation1
Metal bindingi43Zinc 2PROSITE-ProRule annotation1
Metal bindingi59Zinc 2PROSITE-ProRule annotation1
Metal bindingi64Zinc 1PROSITE-ProRule annotation1
Metal bindingi150Zinc 3PROSITE-ProRule annotation1
Metal bindingi155Zinc 3PROSITE-ProRule annotation1
Metal bindingi169Zinc 3PROSITE-ProRule annotation1
Metal bindingi172Zinc 4PROSITE-ProRule annotation1
Metal bindingi187Zinc 4PROSITE-ProRule annotation1
Metal bindingi188Zinc 4PROSITE-ProRule annotation1
Metal bindingi204Zinc 4PROSITE-ProRule annotation1
Metal bindingi209Zinc 3PROSITE-ProRule annotation1

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • zinc ion binding Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-1-binding protein 2
Alternative name(s):
Melusin
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1353420. Itgb1bp2.

Subcellular locationi

GO - Cellular componenti

  • Z disc Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842681 – 350Integrin beta-1-binding protein 2Add BLAST350

Proteomic databases

MaxQBiQ9R000.
PaxDbiQ9R000.
PRIDEiQ9R000.

PTM databases

iPTMnetiQ9R000.
PhosphoSitePlusiQ9R000.

Expressioni

Tissue specificityi

Expressed in skeletal and cardiac muscles but not in other tissues. Is localized in rows flanking the Z line containing alpha-actinin.

Developmental stagei

Detectable in embryo limbs at day 15, reached a maximum in newborn, and declined in adult limb muscles. During heart development level remains steady from embryonic day 15 to adult stage.

Inductioni

During muscle regeneration.

Gene expression databases

BgeeiENSMUSG00000031312.
CleanExiMM_ITGB1BP2.
GenevisibleiQ9R000. MM.

Interactioni

Subunit structurei

Interacts with beta-1 integrin subunit. This interaction is regulated by divalent cations, and it occurs only in absence of calcium.

Binary interactionsi

WithEntry#Exp.IntActNotes
EcdQ9CS742EBI-7922331,EBI-7922565
Hsp90aa1P079015EBI-7922331,EBI-78930

Protein-protein interaction databases

BioGridi205012. 7 interactors.
IntActiQ9R000. 2 interactors.
MINTiMINT-6538502.
STRINGi10090.ENSMUSP00000033674.

Structurei

3D structure databases

ProteinModelPortaliQ9R000.
SMRiQ9R000.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 64CHORD 1PROSITE-ProRule annotationAdd BLAST60
Domaini150 – 209CHORD 2PROSITE-ProRule annotationAdd BLAST60
Domaini216 – 305CSPROSITE-ProRule annotationAdd BLAST90

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi28 – 31SH3-bindingSequence analysis4
Motifi70 – 79SH3-bindingSequence analysis10
Motifi159 – 162SH2-bindingSequence analysis4
Motifi173 – 176SH3-bindingSequence analysis4
Motifi235 – 238SH2-bindingSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 59Cys-richAdd BLAST55
Compositional biasi150 – 204Cys-richAdd BLAST55
Compositional biasi321 – 350Asp/Glu-rich (acidic)Add BLAST30

Domaini

The tail domain binds to the cytoplasmic domain of both integrin beta-1a and beta-1d isoforms. The presence of Ca2+ ions does not prevent binding of a fragment consisting of the second cysteine rich repeat and the tail domain but prevents the binding of the full-length protein.

Sequence similaritiesi

Contains 2 CHORD domains.PROSITE-ProRule annotation
Contains 1 CS domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiKOG1667. Eukaryota.
ENOG410XPV6. LUCA.
GeneTreeiENSGT00390000005180.
HOGENOMiHOG000207093.
HOVERGENiHBG052156.
InParanoidiQ9R000.
KOiK16735.
OMAiTIYGQIP.
OrthoDBiEOG091G0BY9.
PhylomeDBiQ9R000.
TreeFamiTF105394.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007051. CHORD_dom.
IPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF04968. CHORD. 2 hits.
PF04969. CS. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51401. CHORD. 2 hits.
PS51203. CS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLCYNKGC GQHFDPNTNL PDSCRYHPGV PIFHDALKGW SCCRKRTVDF
60 70 80 90 100
SEFLNIKGCT VGLHCAEKLP EVPPQPEGPA TSSLQEQKPL NTIPKSAETL
110 120 130 140 150
FRERPKSEMP PKLLPLLISQ ALGVALEQKE LDQEPGAGLD NSLIWTGSSC
160 170 180 190 200
QNPGCDAVYQ GPESDATPCT YHPGAPRFHE GMKSWSCCGI QTLDFGAFLA
210 220 230 240 250
QPGCRVGRHD WAKQLPASCR HDWHQTDSVV VLTVYGQIPL PAFNWVKASQ
260 270 280 290 300
TELHVHIVFD GNRVFQAQMK LWGVINVEQS SVSLMPSRVE ISLVKADPGS
310 320 330 340 350
WAQLEHPDSL AEKARAGVLL EMDEEESEDS DDDLSWTEEE DEEEEEAMGE
Length:350
Mass (Da):38,768
Last modified:May 1, 2000 - v1
Checksum:i6DEBCCF805DE9289
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148S → F in BAB23069 (PubMed:16141072).Curated1
Sequence conflicti172H → Y in BAB23069 (PubMed:16141072).Curated1
Sequence conflicti182 – 189MKSWSCCG → KKFLKLLC in BAB23069 (PubMed:16141072).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140691 mRNA. Translation: AAF01677.1.
AK003906 mRNA. Translation: BAB23069.1.
BC030035 mRNA. Translation: AAH30035.1.
CCDSiCCDS41079.1.
RefSeqiNP_038740.1. NM_013712.2.
UniGeneiMm.46232.

Genome annotation databases

EnsembliENSMUST00000033674; ENSMUSP00000033674; ENSMUSG00000031312.
GeneIDi26549.
KEGGimmu:26549.
UCSCiuc009txu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140691 mRNA. Translation: AAF01677.1.
AK003906 mRNA. Translation: BAB23069.1.
BC030035 mRNA. Translation: AAH30035.1.
CCDSiCCDS41079.1.
RefSeqiNP_038740.1. NM_013712.2.
UniGeneiMm.46232.

3D structure databases

ProteinModelPortaliQ9R000.
SMRiQ9R000.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205012. 7 interactors.
IntActiQ9R000. 2 interactors.
MINTiMINT-6538502.
STRINGi10090.ENSMUSP00000033674.

PTM databases

iPTMnetiQ9R000.
PhosphoSitePlusiQ9R000.

Proteomic databases

MaxQBiQ9R000.
PaxDbiQ9R000.
PRIDEiQ9R000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033674; ENSMUSP00000033674; ENSMUSG00000031312.
GeneIDi26549.
KEGGimmu:26549.
UCSCiuc009txu.1. mouse.

Organism-specific databases

CTDi26548.
MGIiMGI:1353420. Itgb1bp2.

Phylogenomic databases

eggNOGiKOG1667. Eukaryota.
ENOG410XPV6. LUCA.
GeneTreeiENSGT00390000005180.
HOGENOMiHOG000207093.
HOVERGENiHBG052156.
InParanoidiQ9R000.
KOiK16735.
OMAiTIYGQIP.
OrthoDBiEOG091G0BY9.
PhylomeDBiQ9R000.
TreeFamiTF105394.

Miscellaneous databases

PROiQ9R000.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031312.
CleanExiMM_ITGB1BP2.
GenevisibleiQ9R000. MM.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007051. CHORD_dom.
IPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF04968. CHORD. 2 hits.
PF04969. CS. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51401. CHORD. 2 hits.
PS51203. CS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITBP2_MOUSE
AccessioniPrimary (citable) accession number: Q9R000
Secondary accession number(s): Q9D161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.