Q9QZU9 (UB2L6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin/ISG15-conjugating enzyme E2 L6
EC=6.3.2.19

Alternative name(s):
UbcM8
Ubiquitin carrier protein L6
Ubiquitin-protein ligase L6

Gene names

Name:	Ube2l6
Synonyms:	Ubce8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3 By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RNF19A, RNF19B and RNF144B. Interacts with FLT3 (tyrosine phosphorylated) By similarity.
Post-translational modification	ISGylated By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAD55978.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ISG15-protein conjugation Inferred from direct assay PubMed 16139798. Source: MGI modification-dependent protein catabolic process Inferred from direct assay PubMed 15485925. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ISG15 ligase activity Inferred from direct assay PubMed 16139798. Source: MGI ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Ubiquitin/ISG15-conjugating enzyme E2 L6		PRO_0000082479
Sites
	Active site	86	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	29	1	D → Y in AAD55978. Ref.1
	Sequence conflict	63	1	F → L in AAH08238. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9QZU9 [UniParc]. Last modified May 26, 2009. Version 3. Checksum: 71EEFF050CDE57F1	FASTA	153	17,841
	10 20 30 40 50 60 MMASKRVAKE LESLSKELPP YLRQLSSDDA NVLVWHMLLL PDQLPYGLKA FQVRIDFPRE 70 80 90 100 110 120 YPFKPPTLRF TTKIYHPNVR EDGLVCLPLI SNENWKPYTK PYQVLEALNV LVSKPNLEEP 130 140 150 VRLELADLLT QNPEMFRKKA EEFTLKFGVD RPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Progression from mammary hyperplasia to adenocarcinoma in MMTV-FGF8b transgenic mice is associated with altered expression of CD63, IGFBP7/mac25, alpha-synuclein, and UbcM8." Cook G., Lawshe A., MacArthur C.A. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Bone marrow, Embryo and Embryonic liver.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF159230 mRNA. Translation: AAD55978.1. Different initiation. AK010942 mRNA. Translation: BAB27282.1. AK013452 mRNA. Translation: BAB28861.1. AK152010 mRNA. Translation: BAE30873.1. AK152113 mRNA. Translation: BAE30957.1. AK168695 mRNA. Translation: BAE40539.1. AK171629 mRNA. Translation: BAE42574.1. CH466519 Genomic DNA. Translation: EDL27303.1. BC008238 mRNA. Translation: AAH08238.1.
IPI	IPI00874750.
RefSeq	NP_064333.2. NM_019949.2.
UniGene	Mm.38261.
3D structure databases
ProteinModelPortal	Q9QZU9.
SMR	Q9QZU9. Positions 3-152.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000107264.
PTM databases
PhosphoSite	Q9QZU9.
Proteomic databases
PaxDb	Q9QZU9.
PRIDE	Q9QZU9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102642; ENSMUSP00000099702; ENSMUSG00000027078.
GeneID	56791.
KEGG	mmu:56791.
UCSC	uc012byn.1. mouse.
Organism-specific databases
CTD	9246.
MGI	MGI:1914500. Ube2l6.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00700000104033.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
KO	K04553.
OMA	PEKPPYN.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9QZU9.
Bgee	Q9QZU9.
CleanEx	MM_UBE2L6.
Genevestigator	Q9QZU9.
GermOnline	ENSMUSG00000027078. Mus musculus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	UBE2L6. mouse.
NextBio	313332.
SOURCE	Search...

Entry information

Entry name

UB2L6_MOUSE

Accession

Primary (citable) accession number: Q9QZU9
Secondary accession number(s): Q3U8R0, Q922F1, Q9CQN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	May 26, 2009
Last modified:	April 3, 2013
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program