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Protein

Gamma-butyrobetaine dioxygenase

Gene

Bbox1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-carnitine from gamma-butyrobetaine.

Catalytic activityi

4-trimethylammoniobutanoate + 2-oxoglutarate + O2 = 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
  • L-ascorbate

Pathwayi: carnitine biosynthesis

This protein is involved in the pathway carnitine biosynthesis, which is part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the pathway carnitine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381ZincBy similarity
Metal bindingi40 – 401ZincBy similarity
Metal bindingi43 – 431ZincBy similarity
Metal bindingi82 – 821ZincBy similarity
Metal bindingi202 – 2021Iron; catalyticBy similarity
Metal bindingi204 – 2041Iron; catalyticBy similarity
Metal bindingi347 – 3471Iron; catalyticBy similarity

GO - Molecular functioni

  • gamma-butyrobetaine dioxygenase activity Source: UniProtKB
  • iron ion binding Source: InterPro
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • carnitine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Carnitine biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14431.
BRENDAi1.14.11.1. 5301.
SABIO-RKQ9QZU7.
UniPathwayiUPA00118.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-butyrobetaine dioxygenase (EC:1.14.11.1)
Alternative name(s):
Gamma-butyrobetaine hydroxylase
Short name:
Gamma-BBH
Gamma-butyrobetaine,2-oxoglutarate dioxygenase
Gene namesi
Name:Bbox1
Synonyms:Bbh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619756. Bbox1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Gamma-butyrobetaine dioxygenasePRO_0000207087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei351 – 3511PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QZU7.
PRIDEiQ9QZU7.

PTM databases

iPTMnetiQ9QZU7.
PhosphoSiteiQ9QZU7.

Expressioni

Tissue specificityi

Expressed in the liver and in some extend in the testis and the epididymis.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006197.

Structurei

3D structure databases

ProteinModelPortaliQ9QZU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-BBH/TMLD family.Curated

Phylogenomic databases

eggNOGiKOG3888. Eukaryota.
COG2175. LUCA.
HOGENOMiHOG000231427.
HOVERGENiHBG031125.
InParanoidiQ9QZU7.
KOiK00471.
PhylomeDBiQ9QZU7.
TreeFamiTF313805.

Family and domain databases

InterProiIPR010376. DUF971.
IPR012775. GBBH.
IPR003819. TauD/TfdA-like.
[Graphical view]
PfamiPF06155. DUF971. 1 hit.
PF02668. TauD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02409. carnitine_bodg. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9QZU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCAILKAEA VDGARLMQIF WHDGAESLYP AVWLRDNCQC SDCYLHSAKA
60 70 80 90 100
RKLLLEALDV NIRMDDLTFD QKKVYITWPN GHYSEFEANW LKKRCFSQEA
110 120 130 140 150
RAGLQGELFL PECQYWGSEL QLPTLNFEDV LNDDDHAYKW LSSLKKVGIV
160 170 180 190 200
RLTGAADKRG EIIKLGKRIG FLYLTFYGHT WQVQDKIDAN NVAYTTGKLS
210 220 230 240 250
FHTDYPALHH PPGVQLLHCI KQTVTGGDSE IVDGFNVCQK LKEKNPQAFS
260 270 280 290 300
ILSSTFVDFT DIGVDYCDFS VQSKHKIIEL DDKGQVVRIN FNNATRDTVF
310 320 330 340 350
DVPIERVQPF YAALKEFVDL MNSKEYKYTF KMNPGDVITF DNWRLLHGRR
360 370 380
SYEAGTEISR HLEGAYADWD VVMSRLRILR QRVMNGN
Length:387
Mass (Da):44,546
Last modified:May 1, 2000 - v1
Checksum:i357804BFB35BF274
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF160958 mRNA. Translation: AAD53264.1.
RefSeqiNP_072151.1. NM_022629.1.
UniGeneiRn.16333.

Genome annotation databases

GeneIDi64564.
KEGGirno:64564.
UCSCiRGD:619756. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF160958 mRNA. Translation: AAD53264.1.
RefSeqiNP_072151.1. NM_022629.1.
UniGeneiRn.16333.

3D structure databases

ProteinModelPortaliQ9QZU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006197.

PTM databases

iPTMnetiQ9QZU7.
PhosphoSiteiQ9QZU7.

Proteomic databases

PaxDbiQ9QZU7.
PRIDEiQ9QZU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64564.
KEGGirno:64564.
UCSCiRGD:619756. rat.

Organism-specific databases

CTDi8424.
RGDi619756. Bbox1.

Phylogenomic databases

eggNOGiKOG3888. Eukaryota.
COG2175. LUCA.
HOGENOMiHOG000231427.
HOVERGENiHBG031125.
InParanoidiQ9QZU7.
KOiK00471.
PhylomeDBiQ9QZU7.
TreeFamiTF313805.

Enzyme and pathway databases

UniPathwayiUPA00118.
BioCyciMetaCyc:MONOMER-14431.
BRENDAi1.14.11.1. 5301.
SABIO-RKQ9QZU7.

Miscellaneous databases

NextBioi613498.
PROiQ9QZU7.

Family and domain databases

InterProiIPR010376. DUF971.
IPR012775. GBBH.
IPR003819. TauD/TfdA-like.
[Graphical view]
PfamiPF06155. DUF971. 1 hit.
PF02668. TauD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02409. carnitine_bodg. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the cDNA encoding the rat liver gamma-butyrobetaine hydroxylase."
    Galland S., Le Borgne F., Bouchard F., Georges B., Clouet P., Grand-Jean F., Demarquoy J.
    Biochim. Biophys. Acta 1441:85-92(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Carnitine biosynthesis: identification of the cDNA encoding human gamma-butyrobetaine hydroxylase."
    Vaz F.M., van Gool S., Ofman R., Ijlst L., Wanders R.J.A.
    Biochem. Biophys. Res. Commun. 250:506-510(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBODG_RAT
AccessioniPrimary (citable) accession number: Q9QZU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 1, 2000
Last modified: February 17, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.