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Protein

E3 ubiquitin-protein ligase RNF25

Gene

Rnf25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2. Stimulates transcription mediated by NF-kappa-B (By similarity).By similarity2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri134 – 199RING-typePROSITE-ProRule annotationAdd BLAST66

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NF-kappaB binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF25 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 25
RING finger protein AO7
RING-type E3 ubiquitin transferase RNF25Curated
Gene namesi
Name:Rnf25
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1890215 Rnf25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi134C → S: No E2 binding. Loss of ubiquitination activity. 1 Publication1
Mutagenesisi137C → S: No E2 binding. Loss of ubiquitination activity. 1 Publication1
Mutagenesisi152C → S: No E2 binding. Loss of ubiquitination activity. 1 Publication1
Mutagenesisi157H → C: No E2 binding. Loss of ubiquitination activity. 1 Publication1
Mutagenesisi158C → S: No E2 binding. Loss of ubiquitination activity. 1 Publication1
Mutagenesisi160C → S: No E2 binding. Loss of ubiquitination activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560671 – 456E3 ubiquitin-protein ligase RNF25Add BLAST456

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ9QZR0
MaxQBiQ9QZR0
PaxDbiQ9QZR0
PRIDEiQ9QZR0

PTM databases

iPTMnetiQ9QZR0
PhosphoSitePlusiQ9QZR0

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000026171
CleanExiMM_RNF25
ExpressionAtlasiQ9QZR0 baseline and differential
GenevisibleiQ9QZR0 MM

Interactioni

Subunit structurei

Interacts with UBE2D2, and may also interact with UBE2E1 and UBE2E3. Interacts with RELA (By similarity). Interacts with NKD2.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2083135 interactors.
STRINGi10090.ENSMUSP00000027357

Structurei

3D structure databases

ProteinModelPortaliQ9QZR0
SMRiQ9QZR0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 127RWDPROSITE-ProRule annotationAdd BLAST110

Domaini

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri134 – 199RING-typePROSITE-ProRule annotationAdd BLAST66

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4445 Eukaryota
ENOG4111H5K LUCA
GeneTreeiENSGT00390000001557
HOGENOMiHOG000012992
HOVERGENiHBG055249
InParanoidiQ9QZR0
KOiK10640
OMAiQDSQYVC
OrthoDBiEOG091G0IBN
PhylomeDBiQ9QZR0
TreeFamiTF324097

Family and domain databases

Gene3Di3.10.110.101 hit
3.30.40.101 hit
InterProiView protein in InterPro
IPR006575 RWD-domain
IPR016135 UBQ-conjugating_enzyme/RWD
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QZR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASASTAAG EEDWVLPSEV EVLESIYLDE LQVMKGNGRS PWEIFITLHP
60 70 80 90 100
ATAEVQDSQF VCFTLVLRIP VQYPHEVPQI SIRNPRGLSD EQIHKISQAL
110 120 130 140 150
GHVAKEGLGT AMLYELIEKG KEILTDNNIP HGQCVICLYG FQEKEAFTKT
160 170 180 190 200
PCYHYFHCHC LARYIQHMEQ ELTTQEQEQE RQHVVTKQKA VGVQCPVCRE
210 220 230 240 250
PLVYDLASLK AAPEPQQPME LYQPSAESLR QQEELKLLYQ RQQEKGGIID
260 270 280 290 300
LEAERNRYFI SLQQPPAALE PESAVDVSRE PQPPNALSAE QSTSLADQST
310 320 330 340 350
LPTSLPMTTQ YTYEKTSGAG PNQQRPGETQ KSVLDPPRHG RGSWRQYDRR
360 370 380 390 400
HPKGGECCTP KGTSEIHELP PPEKPLKETV DLKAEPRNKG LTGHPQEKGP
410 420 430 440 450
GSWQGPSARR TRDCARWERS KNRTPGSCYP HLPRGQGAYR SGTRREPLGL

ESEEGS
Length:456
Mass (Da):51,227
Last modified:June 6, 2002 - v2
Checksum:i75FA1351F8E9C4E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182 – 183QH → HD in AAD48057 (PubMed:10500182).Curated2
Sequence conflicti244E → K in BAB22071 (PubMed:16141072).Curated1
Sequence conflicti445R → G in BAB22071 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF171060 mRNA Translation: AAD48057.1
AK002399 mRNA Translation: BAB22071.1
AK171752 mRNA Translation: BAE42650.1
BC022715 mRNA Translation: AAH22715.1
BC048821 mRNA Translation: AAH48821.1
CCDSiCCDS15052.1
RefSeqiNP_001292159.1, NM_001305230.1
NP_067288.2, NM_021313.3
UniGeneiMm.280920
Mm.448381

Genome annotation databases

EnsembliENSMUST00000027357; ENSMUSP00000027357; ENSMUSG00000026171
GeneIDi57751
KEGGimmu:57751
UCSCiuc007bms.3 mouse

Similar proteinsi

Entry informationi

Entry nameiRNF25_MOUSE
AccessioniPrimary (citable) accession number: Q9QZR0
Secondary accession number(s): Q3TAL8, Q9DCW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: February 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome