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Q9QZQ8

- H2AY_MOUSE

UniProt

Q9QZQ8 - H2AY_MOUSE

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Protein

Core histone macro-H2A.1

Gene
H2afy
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.1 Publication

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. dosage compensation Source: MGI
  3. negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: UniProt
  4. nucleosome assembly Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198626. Meiotic synapsis.
REACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.
REACT_27235. Meiotic Recombination.
REACT_75800. Meiotic Synapsis.

Names & Taxonomyi

Protein namesi
Recommended name:
Core histone macro-H2A.1
Short name:
Histone macroH2A1
Short name:
mH2A1
Alternative name(s):
H2A.y
H2A/y
Gene namesi
Name:H2afy
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:1349392. H2afy.

Subcellular locationi

Nucleus. Chromosome
Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin.2 Publications

GO - Cellular componenti

  1. Barr body Source: MGI
  2. centrosome Source: MGI
  3. condensed chromosome Source: MGI
  4. nucleosome Source: UniProtKB-KW
  5. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Core histone macro-H2A.1PRO_0000227904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181N6-methyllysine By similarity
Modified residuei116 – 1161N6-acetyllysine; alternate1 Publication
Cross-linki116 – 116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate By similarity
Cross-linki117 – 117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei123 – 1231N6-acetyllysine1 Publication
Modified residuei129 – 1291Phosphothreonine1 Publication
Modified residuei170 – 1701Phosphoserine By similarity
Modified residuei173 – 1731Phosphoserine By similarity
Modified residuei178 – 1781Phosphothreonine By similarity

Post-translational modificationi

Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin By similarity.

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9QZQ8.
PaxDbiQ9QZQ8.
PRIDEiQ9QZQ8.

PTM databases

PhosphoSiteiQ9QZQ8.

Expressioni

Tissue specificityi

Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.2 Publications

Gene expression databases

BgeeiQ9QZQ8.
CleanExiMM_H2AFY.
GenevestigatoriQ9QZQ8.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 By similarity.2 Publications

Protein-protein interaction databases

BioGridi205061. 1 interaction.
IntActiQ9QZQ8. 3 interactions.
MINTiMINT-4121159.

Structurei

3D structure databases

ProteinModelPortaliQ9QZQ8.
SMRiQ9QZQ8. Positions 10-117, 181-369.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 117116Histone H2AAdd
BLAST
Domaini184 – 370187MacroAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi116 – 16146Lys-richAdd
BLAST

Sequence similaritiesi

Contains 1 histone H2A domain.
Contains 1 Macro domain.

Phylogenomic databases

eggNOGiCOG2110.
GeneTreeiENSGT00740000115227.
HOGENOMiHOG000234653.
HOVERGENiHBG009342.
InParanoidiQ9QZQ8.
KOiK11251.
OMAiQVVQADI.
OrthoDBiEOG71G9VP.
PhylomeDBiQ9QZQ8.
TreeFamiTF332276.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR021171. Core_histone_macro-H2A.
IPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR002119. Histone_H2A.
IPR002589. Macro_dom.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
PF01661. Macro. 1 hit.
[Graphical view]
PIRSFiPIRSF037942. Core_histone_macro-H2A. 1 hit.
PRINTSiPR00620. HISTONEH2A.
SMARTiSM00506. A1pp. 1 hit.
SM00414. H2A. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS51154. MACRO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 2 (identifier: Q9QZQ8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA    50
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI 100
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVAKKTG 150
GKKGARKSKK KQGEVSKAAS ADSTTEGTPT DGFTVLSTKS LFLGQKLNLI 200
HSEISNLAGF EVEAIINPTN ADIDLKDDLG NTLEKKGGKE FVEAVLELRK 250
KNGPLEVAGA AISAGHGLPA KFVIHCNSPV WGADKCEELL EKTVKNCLAL 300
ADDRKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY 350
FMLFDSESIG IYVQEMAKLD AN 372

Note: Major form.

Length:372
Mass (Da):39,735
Last modified:January 23, 2007 - v3
Checksum:i076CF76A34FACAA0
GO
Isoform 1 (identifier: Q9QZQ8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-217: NLIHSEISNLAGFEVEAIIN → QVVQADIASIDSDAVVH
     221-229: ADIDLKDDL → TDFYTGGEV

Show »
Length:369
Mass (Da):39,290
Checksum:i33F123DE5C9C46EC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei198 – 21720NLIHS…EAIIN → QVVQADIASIDSDAVVH in isoform 1. VSP_017596Add
BLAST
Alternative sequencei221 – 2299ADIDLKDDL → TDFYTGGEV in isoform 1. VSP_017597

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF171080 mRNA. Translation: AAD53745.1.
AF171081 mRNA. Translation: AAD53746.1.
AB071988 mRNA. Translation: BAB68541.1.
BC006955 mRNA. Translation: AAH06955.1.
CCDSiCCDS26557.1. [Q9QZQ8-1]
CCDS49278.1. [Q9QZQ8-2]
RefSeqiNP_001152985.1. NM_001159513.1.
NP_001152986.1. NM_001159514.1. [Q9QZQ8-2]
NP_001152987.1. NM_001159515.1.
NP_036145.1. NM_012015.2. [Q9QZQ8-1]
UniGeneiMm.283802.
Mm.478369.

Genome annotation databases

EnsembliENSMUST00000016081; ENSMUSP00000016081; ENSMUSG00000015937. [Q9QZQ8-1]
ENSMUST00000045788; ENSMUSP00000038221; ENSMUSG00000015937. [Q9QZQ8-2]
GeneIDi26914.
KEGGimmu:26914.
UCSCiuc007qsf.2. mouse. [Q9QZQ8-1]
uc007qsg.2. mouse. [Q9QZQ8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF171080 mRNA. Translation: AAD53745.1 .
AF171081 mRNA. Translation: AAD53746.1 .
AB071988 mRNA. Translation: BAB68541.1 .
BC006955 mRNA. Translation: AAH06955.1 .
CCDSi CCDS26557.1. [Q9QZQ8-1 ]
CCDS49278.1. [Q9QZQ8-2 ]
RefSeqi NP_001152985.1. NM_001159513.1.
NP_001152986.1. NM_001159514.1. [Q9QZQ8-2 ]
NP_001152987.1. NM_001159515.1.
NP_036145.1. NM_012015.2. [Q9QZQ8-1 ]
UniGenei Mm.283802.
Mm.478369.

3D structure databases

ProteinModelPortali Q9QZQ8.
SMRi Q9QZQ8. Positions 10-117, 181-369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 205061. 1 interaction.
IntActi Q9QZQ8. 3 interactions.
MINTi MINT-4121159.

PTM databases

PhosphoSitei Q9QZQ8.

Proteomic databases

MaxQBi Q9QZQ8.
PaxDbi Q9QZQ8.
PRIDEi Q9QZQ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000016081 ; ENSMUSP00000016081 ; ENSMUSG00000015937 . [Q9QZQ8-1 ]
ENSMUST00000045788 ; ENSMUSP00000038221 ; ENSMUSG00000015937 . [Q9QZQ8-2 ]
GeneIDi 26914.
KEGGi mmu:26914.
UCSCi uc007qsf.2. mouse. [Q9QZQ8-1 ]
uc007qsg.2. mouse. [Q9QZQ8-2 ]

Organism-specific databases

CTDi 9555.
MGIi MGI:1349392. H2afy.

Phylogenomic databases

eggNOGi COG2110.
GeneTreei ENSGT00740000115227.
HOGENOMi HOG000234653.
HOVERGENi HBG009342.
InParanoidi Q9QZQ8.
KOi K11251.
OMAi QVVQADI.
OrthoDBi EOG71G9VP.
PhylomeDBi Q9QZQ8.
TreeFami TF332276.

Enzyme and pathway databases

Reactomei REACT_198626. Meiotic synapsis.
REACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.
REACT_27235. Meiotic Recombination.
REACT_75800. Meiotic Synapsis.

Miscellaneous databases

ChiTaRSi H2AFY. mouse.
NextBioi 304793.
PROi Q9QZQ8.
SOURCEi Search...

Gene expression databases

Bgeei Q9QZQ8.
CleanExi MM_H2AFY.
Genevestigatori Q9QZQ8.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR021171. Core_histone_macro-H2A.
IPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR002119. Histone_H2A.
IPR002589. Macro_dom.
[Graphical view ]
Pfami PF00125. Histone. 1 hit.
PF01661. Macro. 1 hit.
[Graphical view ]
PIRSFi PIRSF037942. Core_histone_macro-H2A. 1 hit.
PRINTSi PR00620. HISTONEH2A.
SMARTi SM00506. A1pp. 1 hit.
SM00414. H2A. 1 hit.
[Graphical view ]
SUPFAMi SSF47113. SSF47113. 1 hit.
PROSITEi PS51154. MACRO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Messenger RNAs encoding mouse histone macroH2A1 isoforms are expressed at similar levels in male and female cells and result from alternative splicing."
    Rasmussen T.P., Huang T., Mastrangelo M.-A., Loring J., Panning B., Jaenisch R.
    Nucleic Acids Res. 27:3685-3689(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 197-229 (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: 129/SvEvTacfBr.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH SPOP.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals."
    Costanzi C., Pehrson J.R.
    Nature 393:599-601(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "MACROH2A2, a new member of the MACROH2A core histone family."
    Costanzi C., Pehrson J.R.
    J. Biol. Chem. 276:21776-21784(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence."
    Grigoryev S.A., Nikitina T., Pehrson J.R., Singh P.B., Woodcock C.L.
    J. Cell Sci. 117:6153-6162(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. Cited for: INTERACTION WITH HDAC1 AND HDAC2, FUNCTION.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-116 AND LYS-123, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiH2AY_MOUSE
AccessioniPrimary (citable) accession number: Q9QZQ8
Secondary accession number(s): Q91VZ2, Q9QZQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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