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Q9QZQ8

- H2AY_MOUSE

UniProt

Q9QZQ8 - H2AY_MOUSE

Protein

Core histone macro-H2A.1

Gene

H2afy

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.1 Publication

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. dosage compensation Source: MGI
    3. negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: UniProt
    4. nucleosome assembly Source: InterPro

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198626. Meiotic synapsis.
    REACT_200667. NoRC negatively regulates rRNA expression.
    REACT_214440. NoRC negatively regulates rRNA expression.
    REACT_27235. Meiotic Recombination.
    REACT_75800. Meiotic Synapsis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Core histone macro-H2A.1
    Short name:
    Histone macroH2A1
    Short name:
    mH2A1
    Alternative name(s):
    H2A.y
    H2A/y
    Gene namesi
    Name:H2afy
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1349392. H2afy.

    Subcellular locationi

    Nucleus. Chromosome
    Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin.

    GO - Cellular componenti

    1. Barr body Source: MGI
    2. centrosome Source: MGI
    3. condensed chromosome Source: MGI
    4. nucleosome Source: UniProtKB-KW
    5. nucleus Source: MGI

    Keywords - Cellular componenti

    Chromosome, Nucleosome core, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Core histone macro-H2A.1PRO_0000227904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei18 – 181N6-methyllysineBy similarity
    Modified residuei116 – 1161N6-acetyllysine; alternate1 Publication
    Cross-linki116 – 116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
    Cross-linki117 – 117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei123 – 1231N6-acetyllysine1 Publication
    Modified residuei129 – 1291Phosphothreonine1 Publication
    Modified residuei170 – 1701PhosphoserineBy similarity
    Modified residuei173 – 1731PhosphoserineBy similarity
    Modified residuei178 – 1781PhosphothreonineBy similarity

    Post-translational modificationi

    Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin By similarity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ9QZQ8.
    PaxDbiQ9QZQ8.
    PRIDEiQ9QZQ8.

    PTM databases

    PhosphoSiteiQ9QZQ8.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.2 Publications

    Gene expression databases

    BgeeiQ9QZQ8.
    CleanExiMM_H2AFY.
    GenevestigatoriQ9QZQ8.

    Interactioni

    Subunit structurei

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi205061. 1 interaction.
    IntActiQ9QZQ8. 3 interactions.
    MINTiMINT-4121159.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QZQ8.
    SMRiQ9QZQ8. Positions 10-117, 181-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 117116Histone H2AAdd
    BLAST
    Domaini184 – 370187MacroPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi116 – 16146Lys-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 histone H2A domain.Curated
    Contains 1 Macro domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2110.
    GeneTreeiENSGT00740000115227.
    HOGENOMiHOG000234653.
    HOVERGENiHBG009342.
    InParanoidiQ9QZQ8.
    KOiK11251.
    OMAiQVVQADI.
    OrthoDBiEOG71G9VP.
    PhylomeDBiQ9QZQ8.
    TreeFamiTF332276.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR021171. Core_histone_macro-H2A.
    IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR002119. Histone_H2A.
    IPR002589. Macro_dom.
    [Graphical view]
    PfamiPF00125. Histone. 1 hit.
    PF01661. Macro. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037942. Core_histone_macro-H2A. 1 hit.
    PRINTSiPR00620. HISTONEH2A.
    SMARTiSM00506. A1pp. 1 hit.
    SM00414. H2A. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.
    PROSITEiPS51154. MACRO. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: Q9QZQ8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA    50
    AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI 100
    ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVAKKTG 150
    GKKGARKSKK KQGEVSKAAS ADSTTEGTPT DGFTVLSTKS LFLGQKLNLI 200
    HSEISNLAGF EVEAIINPTN ADIDLKDDLG NTLEKKGGKE FVEAVLELRK 250
    KNGPLEVAGA AISAGHGLPA KFVIHCNSPV WGADKCEELL EKTVKNCLAL 300
    ADDRKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY 350
    FMLFDSESIG IYVQEMAKLD AN 372

    Note: Major form.

    Length:372
    Mass (Da):39,735
    Last modified:January 23, 2007 - v3
    Checksum:i076CF76A34FACAA0
    GO
    Isoform 1 (identifier: Q9QZQ8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         198-217: NLIHSEISNLAGFEVEAIIN → QVVQADIASIDSDAVVH
         221-229: ADIDLKDDL → TDFYTGGEV

    Show »
    Length:369
    Mass (Da):39,290
    Checksum:i33F123DE5C9C46EC
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei198 – 21720NLIHS…EAIIN → QVVQADIASIDSDAVVH in isoform 1. 2 PublicationsVSP_017596Add
    BLAST
    Alternative sequencei221 – 2299ADIDLKDDL → TDFYTGGEV in isoform 1. 2 PublicationsVSP_017597

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF171080 mRNA. Translation: AAD53745.1.
    AF171081 mRNA. Translation: AAD53746.1.
    AB071988 mRNA. Translation: BAB68541.1.
    BC006955 mRNA. Translation: AAH06955.1.
    CCDSiCCDS26557.1. [Q9QZQ8-1]
    CCDS49278.1. [Q9QZQ8-2]
    RefSeqiNP_001152985.1. NM_001159513.1.
    NP_001152986.1. NM_001159514.1. [Q9QZQ8-2]
    NP_001152987.1. NM_001159515.1.
    NP_036145.1. NM_012015.2. [Q9QZQ8-1]
    UniGeneiMm.283802.
    Mm.478369.

    Genome annotation databases

    EnsembliENSMUST00000016081; ENSMUSP00000016081; ENSMUSG00000015937. [Q9QZQ8-1]
    ENSMUST00000045788; ENSMUSP00000038221; ENSMUSG00000015937. [Q9QZQ8-2]
    GeneIDi26914.
    KEGGimmu:26914.
    UCSCiuc007qsf.2. mouse. [Q9QZQ8-1]
    uc007qsg.2. mouse. [Q9QZQ8-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF171080 mRNA. Translation: AAD53745.1 .
    AF171081 mRNA. Translation: AAD53746.1 .
    AB071988 mRNA. Translation: BAB68541.1 .
    BC006955 mRNA. Translation: AAH06955.1 .
    CCDSi CCDS26557.1. [Q9QZQ8-1 ]
    CCDS49278.1. [Q9QZQ8-2 ]
    RefSeqi NP_001152985.1. NM_001159513.1.
    NP_001152986.1. NM_001159514.1. [Q9QZQ8-2 ]
    NP_001152987.1. NM_001159515.1.
    NP_036145.1. NM_012015.2. [Q9QZQ8-1 ]
    UniGenei Mm.283802.
    Mm.478369.

    3D structure databases

    ProteinModelPortali Q9QZQ8.
    SMRi Q9QZQ8. Positions 10-117, 181-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 205061. 1 interaction.
    IntActi Q9QZQ8. 3 interactions.
    MINTi MINT-4121159.

    PTM databases

    PhosphoSitei Q9QZQ8.

    Proteomic databases

    MaxQBi Q9QZQ8.
    PaxDbi Q9QZQ8.
    PRIDEi Q9QZQ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000016081 ; ENSMUSP00000016081 ; ENSMUSG00000015937 . [Q9QZQ8-1 ]
    ENSMUST00000045788 ; ENSMUSP00000038221 ; ENSMUSG00000015937 . [Q9QZQ8-2 ]
    GeneIDi 26914.
    KEGGi mmu:26914.
    UCSCi uc007qsf.2. mouse. [Q9QZQ8-1 ]
    uc007qsg.2. mouse. [Q9QZQ8-2 ]

    Organism-specific databases

    CTDi 9555.
    MGIi MGI:1349392. H2afy.

    Phylogenomic databases

    eggNOGi COG2110.
    GeneTreei ENSGT00740000115227.
    HOGENOMi HOG000234653.
    HOVERGENi HBG009342.
    InParanoidi Q9QZQ8.
    KOi K11251.
    OMAi QVVQADI.
    OrthoDBi EOG71G9VP.
    PhylomeDBi Q9QZQ8.
    TreeFami TF332276.

    Enzyme and pathway databases

    Reactomei REACT_198626. Meiotic synapsis.
    REACT_200667. NoRC negatively regulates rRNA expression.
    REACT_214440. NoRC negatively regulates rRNA expression.
    REACT_27235. Meiotic Recombination.
    REACT_75800. Meiotic Synapsis.

    Miscellaneous databases

    ChiTaRSi H2AFY. mouse.
    NextBioi 304793.
    PROi Q9QZQ8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9QZQ8.
    CleanExi MM_H2AFY.
    Genevestigatori Q9QZQ8.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR021171. Core_histone_macro-H2A.
    IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR002119. Histone_H2A.
    IPR002589. Macro_dom.
    [Graphical view ]
    Pfami PF00125. Histone. 1 hit.
    PF01661. Macro. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037942. Core_histone_macro-H2A. 1 hit.
    PRINTSi PR00620. HISTONEH2A.
    SMARTi SM00506. A1pp. 1 hit.
    SM00414. H2A. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    PROSITEi PS51154. MACRO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Messenger RNAs encoding mouse histone macroH2A1 isoforms are expressed at similar levels in male and female cells and result from alternative splicing."
      Rasmussen T.P., Huang T., Mastrangelo M.-A., Loring J., Panning B., Jaenisch R.
      Nucleic Acids Res. 27:3685-3689(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 197-229 (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: 129/SvEvTacfBr.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH SPOP.
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals."
      Costanzi C., Pehrson J.R.
      Nature 393:599-601(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "MACROH2A2, a new member of the MACROH2A core histone family."
      Costanzi C., Pehrson J.R.
      J. Biol. Chem. 276:21776-21784(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence."
      Grigoryev S.A., Nikitina T., Pehrson J.R., Singh P.B., Woodcock C.L.
      J. Cell Sci. 117:6153-6162(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. Cited for: INTERACTION WITH HDAC1 AND HDAC2, FUNCTION.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-116 AND LYS-123, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiH2AY_MOUSE
    AccessioniPrimary (citable) accession number: Q9QZQ8
    Secondary accession number(s): Q91VZ2, Q9QZQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3