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Protein

Protein NOV homolog

Gene

Nov

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival. Acts by binding to integrins or membrane receptors such as NOTCH1. Essential regulator of hematopoietic stem and progenitor cell function. Inhibits myogenic differentiation through the activation of Notch-signaling pathway. Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling. Ligand of integrins ITGAV:ITGB3 and ITGA5:ITGB1, acts directly upon endothelial cells to stimulate pro-angiogenic activities and induces angiogenesis. In endothelial cells, supports cell adhesion, induces directed cell migration (chemotaxis) and promotes cell survival. Plays also a role in cutaneous wound healing acting as integrin receptor ligand. Supports skin fibroblast adhesion through ITGA5:ITGB1 and ITGA6:ITGB1 and induces fibroblast chemotaxis through ITGAV:ITGB5. Seems to enhance bFGF-induced DNA synthesis in fibroblasts (By similarity). Involved in bone regeneration as a negative regulator (By similarity). Enhances the articular chondrocytic phenotype, whereas it repressed the one representing endochondral ossification (PubMed:21871891). Impairs pancreatic beta-cell function, inhibits beta-cell proliferation and insulin secretion (By similarity). Plays a role as negative regulator of endothelial pro-inflammatory activation reducing monocyte adhesion, its anti-inflammatory effects occur secondary to the inhibition of NF-kappaB signaling pathway (By similarity). Contributes to the control and coordination of inflammatory processes in atherosclerosis (By similarity). Attenuates inflammatory pain through regulation of IL1B- and TNF-induced MMP9, MMP2 and CCL2 expression. Inhibits MMP9 expression through ITGB1 engagement (PubMed:22353423).By similarity2 Publications

GO - Molecular functioni

  • growth factor activity Source: RGD
  • heparin binding Source: GO_Central
  • integrin binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NOV homolog
Short name:
NovH
Alternative name(s):
CCN family member 3
Nephroblastoma-overexpressed gene protein homolog
Gene namesi
Name:Nov
Synonyms:Ccn3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621553. Nov.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasm Source: UniProtKB
  • dendrite Source: RGD
  • extracellular matrix Source: RGD
  • extracellular region Source: UniProtKB
  • gap junction Source: UniProtKB
  • intracellular membrane-bounded organelle Source: Ensembl
  • neuronal cell body Source: RGD
  • proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Gap junction, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 351330Protein NOV homologPRO_0000014417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Disulfide bondi258 ↔ 295By similarity
Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence analysis
Disulfide bondi275 ↔ 309By similarity
Disulfide bondi286 ↔ 325By similarity
Disulfide bondi289 ↔ 327By similarity
Disulfide bondi294 ↔ 331By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9QZQ5.
PRIDEiQ9QZQ5.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in neurons of dorsal root ganglia and dorsal horn of the spinal cord (at protein level) (PubMed:22353423). Expressed in astrocytes (at protein level) (PubMed:15213231). In cartilage, dominantly expressed in the chondrocyte territorial matrix (PubMed:21871891).4 Publications

Inductioni

During persistent inflammatory pain the expression levels are down-regulated.1 Publication

Gene expression databases

GenevisibleiQ9QZQ5. RN.

Interactioni

Subunit structurei

Interacts with FBLN1. Interacts (via CTCK domain) with NOTCH1 (via the EGF-like repeat region) (By similarity). Interacts with GJA1/CX43 (PubMed:15181016, PubMed:15213231). Interacts with ITGA5:ITGB1, ITGAV:ITGB3 and ITGAV:ITGB5 (By similarity).By similarity2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011904.

Structurei

3D structure databases

ProteinModelPortaliQ9QZQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 9975IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini102 – 16867VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini199 – 24446TSP type-1PROSITE-ProRule annotationAdd
BLAST
Domaini258 – 33275CTCKPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CCN family.Curated
Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IH0W. Eukaryota.
ENOG4111F77. LUCA.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiQ9QZQ5.
OMAiQCEMVKQ.
OrthoDBiEOG7N8ZXB.
PhylomeDBiQ9QZQ5.
TreeFamiTF326070.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. TSP1_rpt.
IPR001007. VWF_dom.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFLRKQCL CLGFLLLHLL NQVSATLRCP SRCPSQCPSI SPTCAPGVRS
60 70 80 90 100
VLDGCSCCPV CARQRGESCS EMRPCDQSSG LYCDRSADPN NETGICMVPE
110 120 130 140 150
GDNCVFDGVI YRNGEKFEPN CQYHCTCRDG QIGCVPRCQL DVLLPGPDCP
160 170 180 190 200
APKKVAVPGE CCEKWTCGSE EKGTLGGLAL PAYRPEATVG VELSDSSINC
210 220 230 240 250
IEQTTEWSAC SKSCGMGLST RVTNRNLQCE MVKQTRLCMV RPCEQEPGEA
260 270 280 290 300
TDMKGKKCLR TKKSLKSIHL QFKNCTSLYT YKPRFCGICS DGRCCTPFNT
310 320 330 340 350
KTIQVEFQCL PGQIIKKPVM VIGTCTCHSN CPQNNEAFLQ ELELKTSRGE

M
Length:351
Mass (Da):38,510
Last modified:May 1, 2000 - v1
Checksum:i02619707DE7C1BFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF171936 mRNA. Translation: AAD49371.1.
BC072548 mRNA. Translation: AAH72548.1.
RefSeqiNP_110495.1. NM_030868.2.
UniGeneiRn.162955.

Genome annotation databases

EnsembliENSRNOT00000011904; ENSRNOP00000011904; ENSRNOG00000008697.
GeneIDi81526.
KEGGirno:81526.
UCSCiRGD:621553. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF171936 mRNA. Translation: AAD49371.1.
BC072548 mRNA. Translation: AAH72548.1.
RefSeqiNP_110495.1. NM_030868.2.
UniGeneiRn.162955.

3D structure databases

ProteinModelPortaliQ9QZQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011904.

Proteomic databases

PaxDbiQ9QZQ5.
PRIDEiQ9QZQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011904; ENSRNOP00000011904; ENSRNOG00000008697.
GeneIDi81526.
KEGGirno:81526.
UCSCiRGD:621553. rat.

Organism-specific databases

CTDi4856.
RGDi621553. Nov.

Phylogenomic databases

eggNOGiENOG410IH0W. Eukaryota.
ENOG4111F77. LUCA.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiQ9QZQ5.
OMAiQCEMVKQ.
OrthoDBiEOG7N8ZXB.
PhylomeDBiQ9QZQ5.
TreeFamiTF326070.

Miscellaneous databases

NextBioi615061.
PROiQ9QZQ5.

Gene expression databases

GenevisibleiQ9QZQ5. RN.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. TSP1_rpt.
IPR001007. VWF_dom.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nephroblastoma overexpressed gene (NOV) codes for a growth factor that induces protein tyrosine phosphorylation."
    Liu C., Liu X.J., Crowe P.D., Kelner G.S., Fan J., Barry G., Manu F., Ling N., De Souza E.B., Maki R.A.
    Gene 238:471-478(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Connexin43 interacts with NOV: a possible mechanism for negative regulation of cell growth in choriocarcinoma cells."
    Gellhaus A., Dong X., Propson S., Maass K., Klein-Hitpass L., Kibschull M., Traub O., Willecke K., Perbal B., Lye S.J., Winterhager E.
    J. Biol. Chem. 279:36931-36942(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GJA1.
  4. "CCN3 (NOV) interacts with connexin43 in C6 glioma cells: possible mechanism of connexin-mediated growth suppression."
    Fu C.T., Bechberger J.F., Ozog M.A., Perbal B., Naus C.C.
    J. Biol. Chem. 279:36943-36950(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH GJA1, TISSUE SPECIFICITY.
  5. "Novel effects of CCN3 that may direct the differentiation of chondrocytes."
    Janune D., Kubota S., Nishida T., Kawaki H., Perbal B., Iida S., Takigawa M.
    FEBS Lett. 585:3033-3040(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "NOV/CCN3 attenuates inflammatory pain through regulation of matrix metalloproteinases-2 and -9."
    Kular L., Rivat C., Lelongt B., Calmel C., Laurent M., Pohl M., Kitabgi P., Melik-Parsadaniantz S., Martinerie C.
    J. Neuroinflamm. 9:36-36(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY INFLAMMATORY PAIN.

Entry informationi

Entry nameiNOV_RAT
AccessioniPrimary (citable) accession number: Q9QZQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: May 1, 2000
Last modified: January 20, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.