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Protein

Cannabinoid receptor 2

Gene

Cnr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis (By similarity).By similarity

GO - Molecular functioni

  • cannabinoid receptor activity Source: RGD
  • G-protein coupled receptor activity Source: RGD

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: RGD
  • inflammatory response Source: UniProtKB-KW
  • negative regulation of action potential Source: RGD
  • negative regulation of inflammatory response Source: RGD
  • negative regulation of mast cell activation Source: RGD
  • negative regulation of nitric-oxide synthase activity Source: RGD
  • negative regulation of synaptic transmission, GABAergic Source: RGD
  • response to amphetamine Source: RGD
  • response to lipopolysaccharide Source: RGD
  • sensory perception of pain Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Inflammatory response

Enzyme and pathway databases

ReactomeiR-RNO-373076. Class A/1 (Rhodopsin-like receptors).
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Cannabinoid receptor 2
Short name:
CB-2
Short name:
CB2
Short name:
rCB2
Gene namesi
Name:Cnr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi619713. Cnr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333ExtracellularSequence analysisAdd
BLAST
Transmembranei34 – 5926Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini60 – 7112CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9221Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini93 – 10412ExtracellularSequence analysisAdd
BLAST
Transmembranei105 – 12925Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini130 – 14920CytoplasmicSequence analysisAdd
BLAST
Transmembranei150 – 17223Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini173 – 18816ExtracellularSequence analysisAdd
BLAST
Transmembranei189 – 21426Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini215 – 24632CytoplasmicSequence analysisAdd
BLAST
Transmembranei247 – 26721Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini268 – 27912ExtracellularSequence analysisAdd
BLAST
Transmembranei280 – 30122Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini302 – 36059CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • dendrite Source: RGD
  • extrinsic component of cytoplasmic side of plasma membrane Source: RGD
  • integral component of membrane Source: RGD
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • perikaryon Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2111385.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Cannabinoid receptor 2PRO_0000069325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi11 – 111N-linked (GlcNAc...)Sequence analysis
Modified residuei335 – 3351PhosphoserineBy similarity
Modified residuei336 – 3361PhosphoserineBy similarity
Modified residuei338 – 3381PhosphothreonineBy similarity
Modified residuei352 – 3521PhosphoserineBy similarity

Post-translational modificationi

Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9QZN9.

PTM databases

PhosphoSiteiQ9QZN9.

Expressioni

Tissue specificityi

Expressed in spleen and brain by neurons and glial cells (at protein level). Expressed in lung, testis and thymus but not in heart, liver or kidney. Expressed in cerebellum, cortex and brainstem.4 Publications

Gene expression databases

BgeeiENSRNOG00000009260.
ExpressionAtlasiQ9QZN9. baseline and differential.
GenevisibleiQ9QZN9. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012342.

Chemistry

BindingDBiQ9QZN9.

Structurei

3D structure databases

ProteinModelPortaliQ9QZN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiQ9QZN9.
KOiK04278.
OMAiCNFVNFH.
OrthoDBiEOG091G05O3.
PhylomeDBiQ9QZN9.
TreeFamiTF330052.

Family and domain databases

InterProiIPR001551. Canbinoid_rcpt_2.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR22750:SF10. PTHR22750:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00523. CANABINOID2R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QZN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGCRELELT NGSNGGLEFN PMKEYMILSD AQQIAVAVLC TLMGLLSALE
60 70 80 90 100
NVAVLYLILS SQRLRRKPSY LFIGSLAGAD FLASVIFACN FVIFHVFHGV
110 120 130 140 150
DSRNIFLLKI GSVTMTFTAS VGSLLLTAVD RYLCLCYPPT YKALVTRGRA
160 170 180 190 200
LVALGVMWVL SALISYLPLM GWTCCPSPCS ELFPLIPNDY LLGWLLFIAI
210 220 230 240 250
LFSGIIYTYG YVLWKAHQHV ASLAEHQDRQ VPGIARMRLD VRLAKTLGLV
260 270 280 290 300
MAVLLICWFP ALALMGHSLV TTLSDKVKEA FAFCSMLCLV NSMINPIIYA
310 320 330 340 350
LRSGEIRSAA QHCLTGWKKY LQGLGSEGKE EAPKSSVTET EAEVKTTTGP
360
GSRTPGCSNC
Note: No experimental confirmation available.
Length:360
Mass (Da):39,359
Last modified:February 10, 2009 - v3
Checksum:iE523C77055B24BE0
GO
Isoform 2 (identifier: Q9QZN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     344-360: VKTTTGPGSRTPGCSNC → TLVLKDKQEL...PNKAGQSTLL

Show »
Length:410
Mass (Da):45,163
Checksum:iAA1D8C55E78BF8B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241A → T in AAG09710 (PubMed:12084572).Curated
Sequence conflicti224 – 2241A → T in AAG22010 (PubMed:12084572).Curated
Sequence conflicti224 – 2241A → T in AAG22011 (PubMed:12084572).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei344 – 36017VKTTT…GCSNC → TLVLKDKQELGGDCLLRTSS IHSPMLSLADSANRQDVRPH CPEELTWWCSVRRPISLPNK AGQSTLL in isoform 2. 1 PublicationVSP_036231Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176350 Genomic DNA. Translation: AAF08535.1.
AF218846 mRNA. Translation: AAG09710.1.
AF286721 Genomic DNA. Translation: AAG22010.1.
AF286722 Genomic DNA. Translation: AAG22011.1.
RefSeqiNP_001157614.1. NM_001164142.3. [Q9QZN9-1]
NP_001157615.1. NM_001164143.3. [Q9QZN9-1]
NP_065418.3. NM_020543.4. [Q9QZN9-2]
UniGeneiRn.48776.

Genome annotation databases

EnsembliENSRNOT00000012342; ENSRNOP00000012342; ENSRNOG00000009260. [Q9QZN9-2]
ENSRNOT00000074998; ENSRNOP00000066697; ENSRNOG00000009260. [Q9QZN9-1]
GeneIDi57302.
KEGGirno:57302.
UCSCiRGD:619713. rat. [Q9QZN9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176350 Genomic DNA. Translation: AAF08535.1.
AF218846 mRNA. Translation: AAG09710.1.
AF286721 Genomic DNA. Translation: AAG22010.1.
AF286722 Genomic DNA. Translation: AAG22011.1.
RefSeqiNP_001157614.1. NM_001164142.3. [Q9QZN9-1]
NP_001157615.1. NM_001164143.3. [Q9QZN9-1]
NP_065418.3. NM_020543.4. [Q9QZN9-2]
UniGeneiRn.48776.

3D structure databases

ProteinModelPortaliQ9QZN9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012342.

Chemistry

BindingDBiQ9QZN9.
ChEMBLiCHEMBL2111385.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9QZN9.

Proteomic databases

PaxDbiQ9QZN9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012342; ENSRNOP00000012342; ENSRNOG00000009260. [Q9QZN9-2]
ENSRNOT00000074998; ENSRNOP00000066697; ENSRNOG00000009260. [Q9QZN9-1]
GeneIDi57302.
KEGGirno:57302.
UCSCiRGD:619713. rat. [Q9QZN9-1]

Organism-specific databases

CTDi1269.
RGDi619713. Cnr2.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiQ9QZN9.
KOiK04278.
OMAiCNFVNFH.
OrthoDBiEOG091G05O3.
PhylomeDBiQ9QZN9.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiR-RNO-373076. Class A/1 (Rhodopsin-like receptors).
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiQ9QZN9.

Gene expression databases

BgeeiENSRNOG00000009260.
ExpressionAtlasiQ9QZN9. baseline and differential.
GenevisibleiQ9QZN9. RN.

Family and domain databases

InterProiIPR001551. Canbinoid_rcpt_2.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR22750:SF10. PTHR22750:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00523. CANABINOID2R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNR2_RAT
AccessioniPrimary (citable) accession number: Q9QZN9
Secondary accession number(s): Q9EP74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 10, 2009
Last modified: September 7, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.