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Protein

Sodium/potassium/calcium exchanger 1

Gene

Slc24a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca2+ and 1 K+ in exchange for 4 Na+.

GO - Molecular functioni

  • calcium, potassium:sodium antiporter activity Source: RGD
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • calcium ion transmembrane transport Source: RGD
  • response to stimulus Source: UniProtKB-KW
  • sodium ion transmembrane transport Source: GOC
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antiport, Calcium transport, Ion transport, Sensory transduction, Symport, Transport, Vision

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium/calcium exchanger 1
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 1
Retinal rod Na-Ca+K exchanger
Solute carrier family 24 member 1
Gene namesi
Name:Slc24a1
Synonyms:Nckx1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620080. Slc24a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 419419ExtracellularSequence analysisAdd
BLAST
Transmembranei420 – 44021HelicalSequence analysisAdd
BLAST
Topological domaini441 – 46424CytoplasmicSequence analysisAdd
BLAST
Transmembranei465 – 48521HelicalSequence analysisAdd
BLAST
Topological domaini486 – 4916ExtracellularSequence analysis
Transmembranei492 – 51221HelicalSequence analysisAdd
BLAST
Topological domaini513 – 5197CytoplasmicSequence analysis
Transmembranei520 – 54425HelicalSequence analysisAdd
BLAST
Topological domaini545 – 5528ExtracellularSequence analysis
Transmembranei553 – 56917HelicalSequence analysisAdd
BLAST
Topological domaini570 – 989420CytoplasmicSequence analysisAdd
BLAST
Transmembranei990 – 101021HelicalSequence analysisAdd
BLAST
Topological domaini1011 – 10177ExtracellularSequence analysis
Transmembranei1018 – 103821HelicalSequence analysisAdd
BLAST
Topological domaini1039 – 105315CytoplasmicSequence analysisAdd
BLAST
Transmembranei1054 – 107421HelicalSequence analysisAdd
BLAST
Topological domaini1075 – 109218ExtracellularSequence analysisAdd
BLAST
Transmembranei1093 – 111321HelicalSequence analysisAdd
BLAST
Topological domaini1114 – 11218CytoplasmicSequence analysis
Transmembranei1122 – 114221HelicalSequence analysisAdd
BLAST
Topological domaini1143 – 11508ExtracellularSequence analysis
Transmembranei1151 – 117121HelicalSequence analysisAdd
BLAST
Topological domaini1172 – 118110CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intrinsic component of plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11811181Sodium/potassium/calcium exchanger 1PRO_0000223304Add
BLAST
Signal peptidei1 – ?Not cleavedBy similarity

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence analysis
Modified residuei625 – 6251PhosphoserineSequence analysis
Modified residuei690 – 6901PhosphothreonineBy similarity

Post-translational modificationi

The uncleaved signal sequence is required for efficient membrane targeting and proper membrane integration and topology.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9QZM6.
PRIDEiQ9QZM6.

Expressioni

Tissue specificityi

Highly expressed in the eye.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051962.

Structurei

3D structure databases

ProteinModelPortaliQ9QZM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati461 – 50141Alpha-1Add
BLAST
Repeati730 – 741121Add
BLAST
Repeati742 – 754132Add
BLAST
Repeati755 – 766123Add
BLAST
Repeati767 – 778124Add
BLAST
Repeati779 – 791135Add
BLAST
Repeati792 – 804136Add
BLAST
Repeati805 – 817137Add
BLAST
Repeati818 – 830138Add
BLAST
Repeati831 – 843139Add
BLAST
Repeati844 – 8561310Add
BLAST
Repeati857 – 8691311Add
BLAST
Repeati870 – 8811212Add
BLAST
Repeati882 – 8931213Add
BLAST
Repeati894 – 9051214Add
BLAST
Repeati1061 – 109232Alpha-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni730 – 90517614 X approximate tandem repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi952 – 97423Poly-GluAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
HOGENOMiHOG000231933.
HOVERGENiHBG104097.
InParanoidiQ9QZM6.
KOiK13749.
PhylomeDBiQ9QZM6.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR004817. SLC24A1.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 4 hits.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00927. 2A1904. 1 hit.
TIGR00367. TIGR00367. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QZM6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKLIRMGTQ ERRLLRPKRL HWSRLLFFLG MLIIGSTYQH LRRPQNPPSM
60 70 80 90 100
WTKVSSQQPI KLAIRDLPND EITVTDKDPP EASSEVEGEM LAPQDTVIIG
110 120 130 140 150
EAAPSIAMED TPNPPITTKI TPTSLKNNYS PTTAGTRRQK ENIPPTPSRA
160 170 180 190 200
PSHFISTSGR QRVKSYTPKP RGGRKSSSPT HTREEGRMHT PSPAGAPRTI
210 220 230 240 250
SPTSTEKDSE TTATYRMLEP RSERTAGKTS LKRMVPNTRT FLTREVETDL
260 270 280 290 300
VTSPSLVGKN TLGSPRRGER NSSTTPLRAV LQPTPATSEE QVTASIRMGS
310 320 330 340 350
TPATTEGSTA AQRIGNPLSG TSTPAVRIAS VTDREKRPST AHGTLVTPQV
360 370 380 390 400
KAVLTTQVHR CVVVKPAPAV PMTPSPSMTA ILFPEAPTSG PSALPPGWPN
410 420 430 440 450
LHPKAEYPPD LFSVEDRRQG WVVLHIFGMT YVFVALAIVC DEYFVPALGV
460 470 480 490 500
ITDKLQISED VAGATFMAAG GSAPELFTSL IGVFISHSNV GIGTIVGSAV
510 520 530 540 550
FNILFVIGTC ALFSREILNL TWWPLFRDVS FYILDLSMLI VFFLDSLIAW
560 570 580 590 600
WESLLLLLAY ALYVFTMKWN KQIERWVKEQ LSRRPVAKVM ALGDLSKPSD
610 620 630 640 650
GAIEENEQQD NKKLKLPSVL TRGSSSASLH NSIIRSTIYH LMLHSLDPLG
660 670 680 690 700
EARPSKDKQE SLNQEARVLP QTKAESSSDE EEPAELPAVT VTPAPAPEDK
710 720 730 740 750
GDQEEDPGCQ EDVDEAEHRG DMTGEEGERE TEAEGKKDEE GETEAERKED
760 770 780 790 800
GQEEETETKG KEKQEGETES EGKDEQEGET EAEGKEADHE GETEAEGKEV
810 820 830 840 850
EHEGETEAEG TEDEQEGETE AEGKEVEQEG ETEAEGKEVE HEVETEAERK
860 870 880 890 900
ETNHEGETEA EGKEADHEGE TEAEGNVEHQ GETEAEGKVE HEGETEAGEK
910 920 930 940 950
DEHEGQSETQ ADDTEVKDGE GEAEANAEDQ CETAQGEKGA DGGGGSDGGD
960 970 980 990 1000
SEEEEDEEDE EEEEEEEEEE EEEESEEPLS LEWPESRQKQ AIYLFLLPIV
1010 1020 1030 1040 1050
FPLWLTIPDV RRQEARKFFV ITFLGSIIWI AMFSYLMVWW AHQVGETIGI
1060 1070 1080 1090 1100
SEEIMGLTIL AAGTSIPDLI TSVIVARKGL GDMAVSSSVG SNIFDITVGL
1110 1120 1130 1140 1150
PVPWLLFSLI NALQPIPVSS NGLFCAIVLL FLMLLFVIFS IASCKWRMNK
1160 1170 1180
ILGFTMFLLY FVFLVISVML EDRIISCPVS V
Length:1,181
Mass (Da):129,981
Last modified:May 1, 2000 - v1
Checksum:iB063C1C1193696AE
GO
Isoform 2 (identifier: Q9QZM6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     598-710: Missing.

Show »
Length:1,068
Mass (Da):117,757
Checksum:i860796B1423E167B
GO
Isoform 3 (identifier: Q9QZM6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     616-710: Missing.

Show »
Length:1,086
Mass (Da):119,782
Checksum:i43790F23B8664175
GO
Isoform 4 (identifier: Q9QZM6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     652-679: Missing.

Show »
Length:1,153
Mass (Da):126,899
Checksum:i93DD50700AC53DCB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei598 – 710113Missing in isoform 2. CuratedVSP_006161Add
BLAST
Alternative sequencei616 – 71095Missing in isoform 3. CuratedVSP_006162Add
BLAST
Alternative sequencei652 – 67928Missing in isoform 4. CuratedVSP_006163Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176688 mRNA. Translation: AAD53121.1.
U49235 mRNA. Translation: AAB37753.1.
RefSeqiNP_064475.1. NM_020090.1. [Q9QZM6-1]
UniGeneiRn.205732.

Genome annotation databases

GeneIDi56814.
KEGGirno:56814.
UCSCiRGD:620080. rat. [Q9QZM6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176688 mRNA. Translation: AAD53121.1.
U49235 mRNA. Translation: AAB37753.1.
RefSeqiNP_064475.1. NM_020090.1. [Q9QZM6-1]
UniGeneiRn.205732.

3D structure databases

ProteinModelPortaliQ9QZM6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051962.

Proteomic databases

PaxDbiQ9QZM6.
PRIDEiQ9QZM6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56814.
KEGGirno:56814.
UCSCiRGD:620080. rat. [Q9QZM6-1]

Organism-specific databases

CTDi9187.
RGDi620080. Slc24a1.

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
HOGENOMiHOG000231933.
HOVERGENiHBG104097.
InParanoidiQ9QZM6.
KOiK13749.
PhylomeDBiQ9QZM6.

Miscellaneous databases

PROiQ9QZM6.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR004817. SLC24A1.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 4 hits.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00927. 2A1904. 1 hit.
TIGR00367. TIGR00367. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Alternatively spliced isoforms of the rat eye sodium/calcium+potassium exchanger NCKX1."
    Poon S., Leach S., Li X.-F., Tucker J.E., Schnetkamp P.P.M., Lytton J.
    Am. J. Physiol. 278:C651-C660(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: Sprague-Dawley.
    Tissue: Eye.
  2. White K.E., Gesek F.A., Friedman P.A.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1067-1155.

Entry informationi

Entry nameiNCKX1_RAT
AccessioniPrimary (citable) accession number: Q9QZM6
Secondary accession number(s): Q62932
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.