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Q9QZM5

- ABI1_RAT

UniProt

Q9QZM5 - ABI1_RAT

Protein

Abl interactor 1

Gene

Abi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein tyrosine kinase activator activity Source: Ensembl

    GO - Biological processi

    1. cellular component movement Source: Ensembl
    2. lamellipodium morphogenesis Source: Ensembl
    3. megakaryocyte development Source: Ensembl
    4. peptidyl-tyrosine phosphorylation Source: Ensembl
    5. somitogenesis Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Abl interactor 1
    Alternative name(s):
    Abelson interactor 1
    Short name:
    Abi-1
    Eps8 SH3 domain-binding protein
    Short name:
    Eps8-binding protein
    e3B1
    Gene namesi
    Name:Abi1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 17

    Organism-specific databases

    RGDi621008. Abi1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus. Cell projectionlamellipodium. Cell projectionfilopodium. Cell projectiongrowth cone. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cytoplasmcytoskeleton
    Note: Localized to protruding lamellipodia and filopodia tips. May shuttle from the postsynaptic densities to the nucleus.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasm Source: UniProtKB-SubCell
    3. filopodium Source: UniProtKB-SubCell
    4. growth cone Source: UniProtKB-SubCell
    5. lamellipodium Source: UniProtKB-SubCell
    6. nucleus Source: UniProtKB-SubCell
    7. postsynaptic density Source: UniProtKB-SubCell
    8. postsynaptic membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi53 – 531Y → A: Not phosphorylated and perinuclear upon NMDA treatment. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 476475Abl interactor 1PRO_0000191789Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei53 – 531Phosphotyrosine1 Publication
    Modified residuei178 – 1781PhosphoserineBy similarity
    Modified residuei208 – 2081PhosphotyrosineBy similarity
    Modified residuei211 – 2111PhosphoserineBy similarity
    Modified residuei217 – 2171PhosphoserineBy similarity
    Modified residuei220 – 2201PhosphoserineBy similarity
    Modified residuei291 – 2911PhosphoserineBy similarity
    Modified residuei423 – 4231PhosphotyrosineBy similarity
    Modified residuei475 – 4751PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9QZM5.
    PRIDEiQ9QZM5.

    PTM databases

    PhosphoSiteiQ9QZM5.

    Expressioni

    Tissue specificityi

    Widely expressed in brain, high levels detected in cortex, hippocampus and cerebellum (at protein level).1 Publication

    Developmental stagei

    Low protein levels at birth that increase progressively at least until 14 days after birth.1 Publication

    Gene expression databases

    GenevestigatoriQ9QZM5.

    Interactioni

    Subunit structurei

    Interacts with ABL1, ENAH, STX1A, SNAP25, VAMP2, EPS8, SOS1, SOS2, GRB2, SPTA1, NCKAP1/NAP1, the first SH3 domain of NCK1 and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Interacts (via SH3 domain) with SHANK2 and SHANK3, but not SHANK1; the interaction is direct. Interacts with the heterodimer MYC:MAX; the interaction may enhance MYC:MAX transcriptional activity.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MaxP521642EBI-920097,EBI-1184963
    Shank3Q9JLU47EBI-920097,EBI-6271152

    Protein-protein interaction databases

    DIPiDIP-36968N.
    IntActiQ9QZM5. 9 interactions.
    MINTiMINT-4508312.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QZM5.
    SMRiQ9QZM5. Positions 404-471.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 10763t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST
    Domaini414 – 47360SH3PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi332 – 38655Pro-richAdd
    BLAST

    Domaini

    The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.By similarity

    Sequence similaritiesi

    Belongs to the ABI family.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG262939.
    GeneTreeiENSGT00390000003756.
    HOGENOMiHOG000293213.
    HOVERGENiHBG050446.
    OMAiMPMFDDS.
    OrthoDBiEOG7J17ZT.
    PhylomeDBiQ9QZM5.

    Family and domain databases

    InterProiIPR028457. ABI.
    IPR028456. ABI1.
    IPR012849. Abl-interactor_HHR_dom.
    IPR001452. SH3_domain.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PANTHERiPTHR10460. PTHR10460. 1 hit.
    PTHR10460:SF2. PTHR10460:SF2. 1 hit.
    PfamiPF07815. Abi_HHR. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9QZM5-1 [UniParc]FASTAAdd to Basket

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    MAELQMLLEE EIPSGKRALI ESYQNLTRVA DYCENNYIQA TDKRKALEET    50
    KAYTTQSLAS VAYQINALAN NVLQLLDIQA SQLRRMESSI NHISQTVDIH 100
    KEKVARREIG ILTTNKNTSR THKIIAPANM ERPVRYIRKP IDYTVLDDVG 150
    HGVKHGNNQP ARTGTLSRTN PPTQKPPSPP VSGRGTLGRN TPYKTLEPVK 200
    PPTVPNDYMT SPARLGSQHS PGRTASLNQR PRTHSGSSGG SGSRENSGSS 250
    SIGIPIAVPT PSPPTAGPAA PGAAPGSQYG TMTRQISRHN STTSSTSSGG 300
    YRRTPSVTAQ FSAQPHVNGG PLYSQNSISI APPPPPMPQL TPQIPLTGFV 350
    ARVQENIADS PTPPPPPPPD DIPMFDDSPP PPPPPPVDYE DEEAAVVQYS 400
    DPYADGDPAW APKNYIEKVV AIYDYTKDKD DELSFKEGAI IYVIKKNDDG 450
    WFEGVCNRVT GLFPGNYVES IMHYTD 476
    Length:476
    Mass (Da):51,705
    Last modified:January 23, 2007 - v3
    Checksum:iA2B8CC377090BEA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176784 mRNA. Translation: AAD55263.1.
    RefSeqiXP_006254416.1. XM_006254354.1.
    UniGeneiRn.43675.

    Genome annotation databases

    EnsembliENSRNOT00000057572; ENSRNOP00000054381; ENSRNOG00000031325.
    GeneIDi79249.
    KEGGirno:79249.
    UCSCiRGD:621008. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176784 mRNA. Translation: AAD55263.1 .
    RefSeqi XP_006254416.1. XM_006254354.1.
    UniGenei Rn.43675.

    3D structure databases

    ProteinModelPortali Q9QZM5.
    SMRi Q9QZM5. Positions 404-471.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36968N.
    IntActi Q9QZM5. 9 interactions.
    MINTi MINT-4508312.

    PTM databases

    PhosphoSitei Q9QZM5.

    Proteomic databases

    PaxDbi Q9QZM5.
    PRIDEi Q9QZM5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000057572 ; ENSRNOP00000054381 ; ENSRNOG00000031325 .
    GeneIDi 79249.
    KEGGi rno:79249.
    UCSCi RGD:621008. rat.

    Organism-specific databases

    CTDi 10006.
    RGDi 621008. Abi1.

    Phylogenomic databases

    eggNOGi NOG262939.
    GeneTreei ENSGT00390000003756.
    HOGENOMi HOG000293213.
    HOVERGENi HBG050446.
    OMAi MPMFDDS.
    OrthoDBi EOG7J17ZT.
    PhylomeDBi Q9QZM5.

    Enzyme and pathway databases

    Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    PROi Q9QZM5.

    Gene expression databases

    Genevestigatori Q9QZM5.

    Family and domain databases

    InterProi IPR028457. ABI.
    IPR028456. ABI1.
    IPR012849. Abl-interactor_HHR_dom.
    IPR001452. SH3_domain.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    PANTHERi PTHR10460. PTHR10460. 1 hit.
    PTHR10460:SF2. PTHR10460:SF2. 1 hit.
    Pfami PF07815. Abi_HHR. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA sequence of the rat eps8 binding protein (e3B1)."
      Nicolas G., Galand C., Lecomte M.C.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    2. "Localization and phosphorylation of Abl-interactor proteins, Abi-1 and Abi-2, in the developing nervous system."
      Courtney K.D., Grove M., Vandongen H., Vandongen A., LaMantia A.-S., Pendergast A.M.
      Mol. Cell. Neurosci. 16:244-257(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    3. "Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation."
      Proepper C., Johannsen S., Liebau S., Dahl J., Vaida B., Bockmann J., Kreutz M.R., Gundelfinger E.D., Boeckers T.M.
      EMBO J. 26:1397-1409(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN NEUROGENESIS, INTERACTION WITH EPS8; MYC; MAX; SHANK2; SHANK3 AND WASF1, PHOSPHORYLATION AT TYR-53, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF TYR-53.

    Entry informationi

    Entry nameiABI1_RAT
    AccessioniPrimary (citable) accession number: Q9QZM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3