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Q9QZM5

- ABI1_RAT

UniProt

Q9QZM5 - ABI1_RAT

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Protein

Abl interactor 1

Gene
Abi1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein tyrosine kinase activator activity Source: Ensembl

GO - Biological processi

  1. cellular component movement Source: Ensembl
  2. lamellipodium morphogenesis Source: Ensembl
  3. megakaryocyte development Source: Ensembl
  4. peptidyl-tyrosine phosphorylation Source: Ensembl
  5. somitogenesis Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Abl interactor 1
Alternative name(s):
Abelson interactor 1
Short name:
Abi-1
Eps8 SH3 domain-binding protein
Short name:
Eps8-binding protein
e3B1
Gene namesi
Name:Abi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi621008. Abi1.

Subcellular locationi

Cytoplasm By similarity. Nucleus. Cell projectionlamellipodium. Cell projectionfilopodium. Cell projectiongrowth cone. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cytoplasmcytoskeleton
Note: Localized to protruding lamellipodia and filopodia tips. May shuttle from the postsynaptic densities to the nucleus.2 Publications

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB-SubCell
  3. filopodium Source: UniProtKB-SubCell
  4. growth cone Source: UniProtKB-SubCell
  5. lamellipodium Source: UniProtKB-SubCell
  6. nucleus Source: UniProtKB-SubCell
  7. postsynaptic density Source: UniProtKB-SubCell
  8. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi53 – 531Y → A: Not phosphorylated and perinuclear upon NMDA treatment. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 476475Abl interactor 1PRO_0000191789Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei53 – 531Phosphotyrosine1 Publication
Modified residuei178 – 1781Phosphoserine By similarity
Modified residuei208 – 2081Phosphotyrosine By similarity
Modified residuei211 – 2111Phosphoserine By similarity
Modified residuei217 – 2171Phosphoserine By similarity
Modified residuei220 – 2201Phosphoserine By similarity
Modified residuei291 – 2911Phosphoserine By similarity
Modified residuei423 – 4231Phosphotyrosine By similarity
Modified residuei475 – 4751Phosphothreonine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QZM5.
PRIDEiQ9QZM5.

PTM databases

PhosphoSiteiQ9QZM5.

Expressioni

Tissue specificityi

Widely expressed in brain, high levels detected in cortex, hippocampus and cerebellum (at protein level).1 Publication

Developmental stagei

Low protein levels at birth that increase progressively at least until 14 days after birth.1 Publication

Gene expression databases

GenevestigatoriQ9QZM5.

Interactioni

Subunit structurei

Interacts with ABL1, ENAH, STX1A, SNAP25, VAMP2, EPS8, SOS1, SOS2, GRB2, SPTA1, NCKAP1/NAP1, the first SH3 domain of NCK1 and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Interacts (via SH3 domain) with SHANK2 and SHANK3, but not SHANK1; the interaction is direct. Interacts with the heterodimer MYC:MAX; the interaction may enhance MYC:MAX transcriptional activity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MaxP521642EBI-920097,EBI-1184963
Shank3Q9JLU47EBI-920097,EBI-6271152

Protein-protein interaction databases

DIPiDIP-36968N.
IntActiQ9QZM5. 9 interactions.
MINTiMINT-4508312.

Structurei

3D structure databases

ProteinModelPortaliQ9QZM5.
SMRiQ9QZM5. Positions 404-471.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 10763t-SNARE coiled-coil homologyAdd
BLAST
Domaini414 – 47360SH3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi332 – 38655Pro-richAdd
BLAST

Domaini

The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A By similarity.

Sequence similaritiesi

Belongs to the ABI family.
Contains 1 SH3 domain.

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG262939.
GeneTreeiENSGT00390000003756.
HOGENOMiHOG000293213.
HOVERGENiHBG050446.
OMAiMPMFDDS.
OrthoDBiEOG7J17ZT.
PhylomeDBiQ9QZM5.

Family and domain databases

InterProiIPR028457. ABI.
IPR028456. ABI1.
IPR012849. Abl-interactor_HHR_dom.
IPR001452. SH3_domain.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR10460. PTHR10460. 1 hit.
PTHR10460:SF2. PTHR10460:SF2. 1 hit.
PfamiPF07815. Abi_HHR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZM5-1 [UniParc]FASTAAdd to Basket

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MAELQMLLEE EIPSGKRALI ESYQNLTRVA DYCENNYIQA TDKRKALEET    50
KAYTTQSLAS VAYQINALAN NVLQLLDIQA SQLRRMESSI NHISQTVDIH 100
KEKVARREIG ILTTNKNTSR THKIIAPANM ERPVRYIRKP IDYTVLDDVG 150
HGVKHGNNQP ARTGTLSRTN PPTQKPPSPP VSGRGTLGRN TPYKTLEPVK 200
PPTVPNDYMT SPARLGSQHS PGRTASLNQR PRTHSGSSGG SGSRENSGSS 250
SIGIPIAVPT PSPPTAGPAA PGAAPGSQYG TMTRQISRHN STTSSTSSGG 300
YRRTPSVTAQ FSAQPHVNGG PLYSQNSISI APPPPPMPQL TPQIPLTGFV 350
ARVQENIADS PTPPPPPPPD DIPMFDDSPP PPPPPPVDYE DEEAAVVQYS 400
DPYADGDPAW APKNYIEKVV AIYDYTKDKD DELSFKEGAI IYVIKKNDDG 450
WFEGVCNRVT GLFPGNYVES IMHYTD 476
Length:476
Mass (Da):51,705
Last modified:January 23, 2007 - v3
Checksum:iA2B8CC377090BEA6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF176784 mRNA. Translation: AAD55263.1.
RefSeqiXP_006254416.1. XM_006254354.1.
UniGeneiRn.43675.

Genome annotation databases

EnsembliENSRNOT00000057572; ENSRNOP00000054381; ENSRNOG00000031325.
GeneIDi79249.
KEGGirno:79249.
UCSCiRGD:621008. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF176784 mRNA. Translation: AAD55263.1 .
RefSeqi XP_006254416.1. XM_006254354.1.
UniGenei Rn.43675.

3D structure databases

ProteinModelPortali Q9QZM5.
SMRi Q9QZM5. Positions 404-471.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36968N.
IntActi Q9QZM5. 9 interactions.
MINTi MINT-4508312.

PTM databases

PhosphoSitei Q9QZM5.

Proteomic databases

PaxDbi Q9QZM5.
PRIDEi Q9QZM5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000057572 ; ENSRNOP00000054381 ; ENSRNOG00000031325 .
GeneIDi 79249.
KEGGi rno:79249.
UCSCi RGD:621008. rat.

Organism-specific databases

CTDi 10006.
RGDi 621008. Abi1.

Phylogenomic databases

eggNOGi NOG262939.
GeneTreei ENSGT00390000003756.
HOGENOMi HOG000293213.
HOVERGENi HBG050446.
OMAi MPMFDDS.
OrthoDBi EOG7J17ZT.
PhylomeDBi Q9QZM5.

Enzyme and pathway databases

Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.

Miscellaneous databases

PROi Q9QZM5.

Gene expression databases

Genevestigatori Q9QZM5.

Family and domain databases

InterProi IPR028457. ABI.
IPR028456. ABI1.
IPR012849. Abl-interactor_HHR_dom.
IPR001452. SH3_domain.
IPR000727. T_SNARE_dom.
[Graphical view ]
PANTHERi PTHR10460. PTHR10460. 1 hit.
PTHR10460:SF2. PTHR10460:SF2. 1 hit.
Pfami PF07815. Abi_HHR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA sequence of the rat eps8 binding protein (e3B1)."
    Nicolas G., Galand C., Lecomte M.C.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Localization and phosphorylation of Abl-interactor proteins, Abi-1 and Abi-2, in the developing nervous system."
    Courtney K.D., Grove M., Vandongen H., Vandongen A., LaMantia A.-S., Pendergast A.M.
    Mol. Cell. Neurosci. 16:244-257(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation."
    Proepper C., Johannsen S., Liebau S., Dahl J., Vaida B., Bockmann J., Kreutz M.R., Gundelfinger E.D., Boeckers T.M.
    EMBO J. 26:1397-1409(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEUROGENESIS, INTERACTION WITH EPS8; MYC; MAX; SHANK2; SHANK3 AND WASF1, PHOSPHORYLATION AT TYR-53, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF TYR-53.

Entry informationi

Entry nameiABI1_RAT
AccessioniPrimary (citable) accession number: Q9QZM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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