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Q9QZL6

- UBP21_MOUSE

UniProt

Q9QZL6 - UBP21_MOUSE

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Protein

Ubiquitin carboxyl-terminal hydrolase 21

Gene
Usp21, Usp23
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211Nucleophile
Metal bindingi385 – 3851Zinc By similarity
Metal bindingi388 – 3881Zinc By similarity
Metal bindingi438 – 4381Zinc By similarity
Metal bindingi441 – 4411Zinc By similarity
Active sitei519 – 5191Proton acceptor By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. NEDD8-specific protease activity Source: UniProtKB
  4. transcription coactivator activity Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB
  6. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. histone deubiquitination Source: UniProtKB
  2. positive regulation of transcription, DNA-templated Source: UniProtKB
  3. transcription, DNA-templated Source: UniProtKB-KW
  4. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.034.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 21 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21
Gene namesi
Name:Usp21
Synonyms:Usp23
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1353665. Usp21.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi221 – 2211C → A: Abolishes ability to deubiquitinate histone H2A and ability to regulate transcription. 1 Publication
Mutagenesisi519 – 5191H → A: Abolishes ability to deubiquitinate histone H2A and ability to regulate transcription. 1 Publication
Mutagenesisi535 – 5351D → N: Abolishes ability to deubiquitinate histone H2A and ability to regulate transcription. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Ubiquitin carboxyl-terminal hydrolase 21PRO_0000080649Add
BLAST

Proteomic databases

PaxDbiQ9QZL6.
PRIDEiQ9QZL6.

PTM databases

PhosphoSiteiQ9QZL6.

Expressioni

Gene expression databases

BgeeiQ9QZL6.
CleanExiMM_USP21.
GenevestigatoriQ9QZL6.

Interactioni

Protein-protein interaction databases

BioGridi206018. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9QZL6.
SMRiQ9QZL6. Positions 202-560.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 559348USPAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 15219Nuclear export signal By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00750000117710.
HOGENOMiHOG000231498.
HOVERGENiHBG011164.
KOiK11833.
OrthoDBiEOG7FR7GN.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QZL6-1 [UniParc]FASTAAdd to Basket

« Hide

MPQASEHRLG RTREPPVNVQ PRVGAKIPFP PRARSKERRN PVPGPNSMLR    50
PLPPRPGPPD ERLKKLELGR GRTSGSRPRG PLRADHGVPL PGSPPPAVAL 100
PLPSRTNLAR SKSVSSGDLR PMGIALGGHR GAGELGAALS RLALRPEPPT 150
LRRSTSLRRL GGFPGPPTLL SIRTEPPTSH GSFHMISARP SEPFYSDDKM 200
AHHTLLLGSG HVGLRNLGNT CFLNAVLQCL SSTRPLRDFC LRRDFRQEVP 250
GGGRAQELTE AFADVIGALW HPDSCEAVNP TRFRAVFQKY VPSFSGYSQQ 300
DAQEFLKLLM ERLHLEINRR GRRAPPILAS GPVPSPPRRG GGALHEEPEL 350
SDDDRANLMW KRYLEREDSK IVDLFVGQLK SCLKCQACGY RSTTFEVFCD 400
LSLPIPKKGF AGGKVSLRDC FSLFTKEEEL ESENAPVCDR CRQKTRSTKK 450
LTVQRFPRIL VLHLNRFSTS RGSIKKSSVG VDFPLQRLSL GDFASDKAGS 500
PVYQLYALCN HSGSVHYGHY TALCRCQTGW HVYNDSRVSP VSENQVASSE 550
GYVLFYQLMQ EPLRCL 566
Length:566
Mass (Da):62,673
Last modified:May 1, 2000 - v1
Checksum:iF2E1828E9BDB1AFD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2201T → TLPQ in BAB27431. 1 Publication
Sequence conflicti338 – 3381R → C in BAB27431. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF177759 mRNA. Translation: AAD54322.1.
AK011148 mRNA. Translation: BAB27431.1.
BC021903 mRNA. Translation: AAH21903.1.
CCDSiCCDS35774.1.
RefSeqiNP_038947.2. NM_013919.4.
UniGeneiMm.27510.

Genome annotation databases

EnsembliENSMUST00000111306; ENSMUSP00000106938; ENSMUSG00000053483.
GeneIDi30941.
KEGGimmu:30941.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF177759 mRNA. Translation: AAD54322.1 .
AK011148 mRNA. Translation: BAB27431.1 .
BC021903 mRNA. Translation: AAH21903.1 .
CCDSi CCDS35774.1.
RefSeqi NP_038947.2. NM_013919.4.
UniGenei Mm.27510.

3D structure databases

ProteinModelPortali Q9QZL6.
SMRi Q9QZL6. Positions 202-560.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 206018. 3 interactions.

Protein family/group databases

MEROPSi C19.034.

PTM databases

PhosphoSitei Q9QZL6.

Proteomic databases

PaxDbi Q9QZL6.
PRIDEi Q9QZL6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000111306 ; ENSMUSP00000106938 ; ENSMUSG00000053483 .
GeneIDi 30941.
KEGGi mmu:30941.

Organism-specific databases

CTDi 27005.
MGIi MGI:1353665. Usp21.

Phylogenomic databases

eggNOGi COG5533.
GeneTreei ENSGT00750000117710.
HOGENOMi HOG000231498.
HOVERGENi HBG011164.
KOi K11833.
OrthoDBi EOG7FR7GN.

Miscellaneous databases

NextBioi 307382.
PROi Q9QZL6.
SOURCEi Search...

Gene expression databases

Bgeei Q9QZL6.
CleanExi MM_USP21.
Genevestigatori Q9QZL6.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing, tissue distribution and chromosomal assignment of a novel ubiquitin-specific protease USP23."
    Smith T.S., Southan C.
    Biochim. Biophys. Acta 1490:184-188(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. "Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation."
    Nakagawa T., Kajitani T., Togo S., Masuko N., Ohdan H., Hishikawa Y., Koji T., Matsuyama T., Ikura T., Muramatsu M., Ito T.
    Genes Dev. 22:37-49(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF CYS-221; HIS-519 AND ASP-535.

Entry informationi

Entry nameiUBP21_MOUSE
AccessioniPrimary (citable) accession number: Q9QZL6
Secondary accession number(s): Q9D0R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3