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Protein

Ubiquitin carboxyl-terminal hydrolase 21

Gene

Usp21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211Nucleophile
Metal bindingi385 – 3851ZincBy similarity
Metal bindingi388 – 3881ZincBy similarity
Metal bindingi438 – 4381ZincBy similarity
Metal bindingi441 – 4411ZincBy similarity
Active sitei519 – 5191Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 21 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21
Gene namesi
Name:Usp21
Synonyms:Usp23
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1353665. Usp21.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi221 – 2211C → A: Abolishes ability to deubiquitinate histone H2A and ability to regulate transcription. 1 Publication
Mutagenesisi519 – 5191H → A: Abolishes ability to deubiquitinate histone H2A and ability to regulate transcription. 1 Publication
Mutagenesisi535 – 5351D → N: Abolishes ability to deubiquitinate histone H2A and ability to regulate transcription. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Ubiquitin carboxyl-terminal hydrolase 21PRO_0000080649Add
BLAST

Proteomic databases

PaxDbiQ9QZL6.
PRIDEiQ9QZL6.

PTM databases

PhosphoSiteiQ9QZL6.

Expressioni

Gene expression databases

BgeeiQ9QZL6.
CleanExiMM_USP21.
ExpressionAtlasiQ9QZL6. baseline and differential.
GenevisibleiQ9QZL6. MM.

Interactioni

Protein-protein interaction databases

BioGridi206018. 3 interactions.
STRINGi10090.ENSMUSP00000064002.

Structurei

3D structure databases

ProteinModelPortaliQ9QZL6.
SMRiQ9QZL6. Positions 202-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 559348USPAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 15219Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP21 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000231498.
HOVERGENiHBG011164.
InParanoidiQ9QZL6.
KOiK11833.
OrthoDBiEOG7FR7GN.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QZL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQASEHRLG RTREPPVNVQ PRVGAKIPFP PRARSKERRN PVPGPNSMLR
60 70 80 90 100
PLPPRPGPPD ERLKKLELGR GRTSGSRPRG PLRADHGVPL PGSPPPAVAL
110 120 130 140 150
PLPSRTNLAR SKSVSSGDLR PMGIALGGHR GAGELGAALS RLALRPEPPT
160 170 180 190 200
LRRSTSLRRL GGFPGPPTLL SIRTEPPTSH GSFHMISARP SEPFYSDDKM
210 220 230 240 250
AHHTLLLGSG HVGLRNLGNT CFLNAVLQCL SSTRPLRDFC LRRDFRQEVP
260 270 280 290 300
GGGRAQELTE AFADVIGALW HPDSCEAVNP TRFRAVFQKY VPSFSGYSQQ
310 320 330 340 350
DAQEFLKLLM ERLHLEINRR GRRAPPILAS GPVPSPPRRG GGALHEEPEL
360 370 380 390 400
SDDDRANLMW KRYLEREDSK IVDLFVGQLK SCLKCQACGY RSTTFEVFCD
410 420 430 440 450
LSLPIPKKGF AGGKVSLRDC FSLFTKEEEL ESENAPVCDR CRQKTRSTKK
460 470 480 490 500
LTVQRFPRIL VLHLNRFSTS RGSIKKSSVG VDFPLQRLSL GDFASDKAGS
510 520 530 540 550
PVYQLYALCN HSGSVHYGHY TALCRCQTGW HVYNDSRVSP VSENQVASSE
560
GYVLFYQLMQ EPLRCL
Length:566
Mass (Da):62,673
Last modified:May 1, 2000 - v1
Checksum:iF2E1828E9BDB1AFD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2201T → TLPQ in BAB27431 (PubMed:16141072).Curated
Sequence conflicti338 – 3381R → C in BAB27431 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177759 mRNA. Translation: AAD54322.1.
AK011148 mRNA. Translation: BAB27431.1.
BC021903 mRNA. Translation: AAH21903.1.
CCDSiCCDS35774.1.
RefSeqiNP_038947.2. NM_013919.4.
UniGeneiMm.27510.

Genome annotation databases

GeneIDi30941.
KEGGimmu:30941.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177759 mRNA. Translation: AAD54322.1.
AK011148 mRNA. Translation: BAB27431.1.
BC021903 mRNA. Translation: AAH21903.1.
CCDSiCCDS35774.1.
RefSeqiNP_038947.2. NM_013919.4.
UniGeneiMm.27510.

3D structure databases

ProteinModelPortaliQ9QZL6.
SMRiQ9QZL6. Positions 202-560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206018. 3 interactions.
STRINGi10090.ENSMUSP00000064002.

PTM databases

PhosphoSiteiQ9QZL6.

Proteomic databases

PaxDbiQ9QZL6.
PRIDEiQ9QZL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi30941.
KEGGimmu:30941.

Organism-specific databases

CTDi27005.
MGIiMGI:1353665. Usp21.

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000231498.
HOVERGENiHBG011164.
InParanoidiQ9QZL6.
KOiK11833.
OrthoDBiEOG7FR7GN.

Miscellaneous databases

NextBioi307382.
PROiQ9QZL6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QZL6.
CleanExiMM_USP21.
ExpressionAtlasiQ9QZL6. baseline and differential.
GenevisibleiQ9QZL6. MM.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing, tissue distribution and chromosomal assignment of a novel ubiquitin-specific protease USP23."
    Smith T.S., Southan C.
    Biochim. Biophys. Acta 1490:184-188(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. "Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation."
    Nakagawa T., Kajitani T., Togo S., Masuko N., Ohdan H., Hishikawa Y., Koji T., Matsuyama T., Ikura T., Muramatsu M., Ito T.
    Genes Dev. 22:37-49(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF CYS-221; HIS-519 AND ASP-535.

Entry informationi

Entry nameiUBP21_MOUSE
AccessioniPrimary (citable) accession number: Q9QZL6
Secondary accession number(s): Q9D0R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: June 24, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.