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Protein

Carbohydrate sulfotransferase 3

Gene

Chst3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 1437PAPSBy similarity
Nucleotide bindingi297 – 3059PAPSBy similarity

GO - Molecular functioni

  1. chondroitin 6-sulfotransferase activity Source: UniProtKB-EC
  2. proteoglycan sulfotransferase activity Source: RGD

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. peripheral nervous system axon regeneration Source: RGD
  3. positive regulation of cellular component movement Source: RGD
  4. sulfur compound metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BRENDAi2.8.2.17. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 3 (EC:2.8.2.17)
Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name:
C6ST-1
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
Short name:
GST-0
Gene namesi
Name:Chst3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620355. Chst3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei20 – 3819Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini39 – 474436LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Carbohydrate sulfotransferase 3PRO_0000085190Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi459 – 4591N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9QZL2.

PTM databases

PhosphoSiteiQ9QZL2.

Expressioni

Gene expression databases

GenevestigatoriQ9QZL2.

Interactioni

Protein-protein interaction databases

MINTiMINT-4998399.
STRINGi10116.ENSRNOP00000000697.

Structurei

3D structure databases

ProteinModelPortaliQ9QZL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiQ9QZL2.
KOiK01020.
PhylomeDBiQ9QZL2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9QZL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKGLALPQD CRDLVHNLKI RGRYVLFLAF VVIVFIFIEK ENKIISRVSD
60 70 80 90 100
KLKQIPQFVA DANSTDPALL LSENASLLSL SELDSTFSHL RSRLHNLSLQ
110 120 130 140 150
LGIAPAMEAQ GQEAVAEKPS QQAGAGTRRH VLLMATTRTG SSFVGEFFNQ
160 170 180 190 200
QGNIFYLFEP LWHIERTVFF QQGGASAAGS ALVYRDVLKQ LLLCDLYVLE
210 220 230 240 250
PFISPPPEDH LTQFLFRRGS SRSLCEDPVC TPFVKKVFEK YHCRNRHCGP
260 270 280 290 300
LNVTLAAEAC RRKDHMALKV VRIRQLEFLQ PLAEDPRLDL RVIQLVRDPR
310 320 330 340 350
AVLASRMVAF AGKYESWKKW LSEGQDQLSE NEVQRLRGNC ENIRLSAELG
360 370 380 390 400
LRQPAWLRGR YMLVRYEDVA RRPLQKAREM YRSAGIPLTP QVEDWIQKNT
410 420 430 440 450
QAARDSSDVY STQKNSSEQF EKWRFSIPFK LAQVVQAVCG PAMHLFGHKL
460 470
AKDTASLTNR SISLLEERGT FWVT
Length:474
Mass (Da):54,018
Last modified:March 1, 2001 - v2
Checksum:i83EC94C3894CE77E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178689 mRNA. Translation: AAD54386.2.
RefSeqiNP_445860.1. NM_053408.1.
UniGeneiRn.64646.

Genome annotation databases

GeneIDi84468.
KEGGirno:84468.
UCSCiRGD:620355. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178689 mRNA. Translation: AAD54386.2.
RefSeqiNP_445860.1. NM_053408.1.
UniGeneiRn.64646.

3D structure databases

ProteinModelPortaliQ9QZL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4998399.
STRINGi10116.ENSRNOP00000000697.

PTM databases

PhosphoSiteiQ9QZL2.

Proteomic databases

PRIDEiQ9QZL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84468.
KEGGirno:84468.
UCSCiRGD:620355. rat.

Organism-specific databases

CTDi9469.
RGDi620355. Chst3.

Phylogenomic databases

eggNOGiNOG80862.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiQ9QZL2.
KOiK01020.
PhylomeDBiQ9QZL2.

Enzyme and pathway databases

BRENDAi2.8.2.17. 5301.

Miscellaneous databases

NextBioi616944.
PROiQ9QZL2.

Gene expression databases

GenevestigatoriQ9QZL2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Chondroitin 6-sulfotransferase of rat sciatic nerve."
    Li X., Kwok C.-F., Shum K.-Y.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiCHST3_RAT
AccessioniPrimary (citable) accession number: Q9QZL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.