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Q9QZL2 (CHST3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbohydrate sulfotransferase 3

EC=2.8.2.17
Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name=C6ST-1
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
Short name=GST-0
Gene names
Name:Chst3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen By similarity.

Catalytic activity

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Carbohydrate sulfotransferase 3
PRO_0000085190

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 3819Helical; Signal-anchor for type II membrane protein; Potential
Topological domain39 – 474436Lumenal Potential
Nucleotide binding137 – 1437PAPS By similarity
Nucleotide binding297 – 3059PAPS By similarity

Amino acid modifications

Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation2521N-linked (GlcNAc...) Potential
Glycosylation4151N-linked (GlcNAc...) Potential
Glycosylation4591N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9QZL2 [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: 83EC94C3894CE77E

FASTA47454,018
        10         20         30         40         50         60 
MEKGLALPQD CRDLVHNLKI RGRYVLFLAF VVIVFIFIEK ENKIISRVSD KLKQIPQFVA 

        70         80         90        100        110        120 
DANSTDPALL LSENASLLSL SELDSTFSHL RSRLHNLSLQ LGIAPAMEAQ GQEAVAEKPS 

       130        140        150        160        170        180 
QQAGAGTRRH VLLMATTRTG SSFVGEFFNQ QGNIFYLFEP LWHIERTVFF QQGGASAAGS 

       190        200        210        220        230        240 
ALVYRDVLKQ LLLCDLYVLE PFISPPPEDH LTQFLFRRGS SRSLCEDPVC TPFVKKVFEK 

       250        260        270        280        290        300 
YHCRNRHCGP LNVTLAAEAC RRKDHMALKV VRIRQLEFLQ PLAEDPRLDL RVIQLVRDPR 

       310        320        330        340        350        360 
AVLASRMVAF AGKYESWKKW LSEGQDQLSE NEVQRLRGNC ENIRLSAELG LRQPAWLRGR 

       370        380        390        400        410        420 
YMLVRYEDVA RRPLQKAREM YRSAGIPLTP QVEDWIQKNT QAARDSSDVY STQKNSSEQF 

       430        440        450        460        470 
EKWRFSIPFK LAQVVQAVCG PAMHLFGHKL AKDTASLTNR SISLLEERGT FWVT 

« Hide

References

[1]"Chondroitin 6-sulfotransferase of rat sciatic nerve."
Li X., Kwok C.-F., Shum K.-Y.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF178689 mRNA. Translation: AAD54386.2.
RefSeqNP_445860.1. NM_053408.1.
UniGeneRn.64646.

3D structure databases

ProteinModelPortalQ9QZL2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4998399.
STRING10116.ENSRNOP00000000697.

PTM databases

PhosphoSiteQ9QZL2.

Proteomic databases

PRIDEQ9QZL2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID84468.
KEGGrno:84468.
UCSCRGD:620355. rat.

Organism-specific databases

CTD9469.
RGD620355. Chst3.

Phylogenomic databases

eggNOGNOG80862.
HOGENOMHOG000261614.
HOVERGENHBG106811.
InParanoidQ9QZL2.
KOK01020.
PhylomeDBQ9QZL2.

Enzyme and pathway databases

BRENDA2.8.2.17. 5301.

Gene expression databases

GenevestigatorQ9QZL2.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMSSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

NextBio616944.
PROQ9QZL2.

Entry information

Entry nameCHST3_RAT
AccessionPrimary (citable) accession number: Q9QZL2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families