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Protein

Deoxyribonuclease-2-beta

Gene

Dnase2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes DNA under acidic conditions. Does not require divalent cations for activity. Participates in the degradation of nuclear DNA during lens cell differentiation.1 Publication

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

GO - Molecular functioni

  • deoxyribonuclease II activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.22.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-2-beta (EC:3.1.22.1)
Alternative name(s):
DNase II-like acid DNase
DNase2-like acid DNase
Deoxyribonuclease II beta
Short name:
DNase II beta
Endonuclease DLAD
Gene namesi
Name:Dnase2b
Synonyms:Dlad
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70901. Dnase2b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 356334Deoxyribonuclease-2-betaPRO_0000007297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9QZK9.
PRIDEiQ9QZK9.

Expressioni

Tissue specificityi

Liver specific.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021861.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase II family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiQ9QZK9.
KOiK01158.
PhylomeDBiQ9QZK9.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQPLKAAL PLLFVALSGV LGTPVISCIN EDGKAVDWFA FYKLPRRTSR
60 70 80 90 100
GGTGMGLDYL YLDSTMRTWS KSHHLINSSR SSLGRTLEQL YEAHNAKNDT
110 120 130 140 150
AYLIYNDAVP ASVNYSGNYG HAKGLLVWNR VQGFWLIHSI PKFPPVPEKG
160 170 180 190 200
YEYPSSGRQY AQSGLCITLK YSQYETIDSQ LLVFQPNIYS CFIPNIFRWE
210 220 230 240 250
LIHMPQMCAK SSASKIPSRR LTVLQSAQGL NFLHFAKSTF YTDDIFAAWI
260 270 280 290 300
AQKLKVHLLV ESWQRKNHEL PSNCSLPYHV YNIKAIRGPL QSDFPSHHDH
310 320 330 340 350
SKWCVSTKDS QARWTCIGDL NRSPHQALRS GGFICSKNRY IYQSFDRLVS

HYASCN
Length:356
Mass (Da):40,472
Last modified:May 1, 2000 - v1
Checksum:i5CBC259F42DD6741
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178974 mRNA. Translation: AAF13596.1.
PIRiJC7131.
RefSeqiNP_067696.1. NM_021664.1.
UniGeneiRn.41640.

Genome annotation databases

GeneIDi59296.
KEGGirno:59296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178974 mRNA. Translation: AAF13596.1.
PIRiJC7131.
RefSeqiNP_067696.1. NM_021664.1.
UniGeneiRn.41640.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021861.

Proteomic databases

PaxDbiQ9QZK9.
PRIDEiQ9QZK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi59296.
KEGGirno:59296.

Organism-specific databases

CTDi58511.
RGDi70901. Dnase2b.

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiQ9QZK9.
KOiK01158.
PhylomeDBiQ9QZK9.

Enzyme and pathway databases

BRENDAi3.1.22.1. 5301.

Miscellaneous databases

PROiQ9QZK9.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a cDNA encoding a rat DNase II-like acid DNase."
    Tanuma S., Shiokawa D.
    Biochem. Biophys. Res. Commun. 265:395-399(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Liver.
  2. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 48-71 AND 211-219, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiDNS2B_RAT
AccessioniPrimary (citable) accession number: Q9QZK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibited by aurintricarboxylic acid and Zn2+.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.