Q9QZK9 (DNS2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyribonuclease-2-beta
EC=3.1.22.1

Alternative name(s):
DNase II-like acid DNase
DNase2-like acid DNase
Deoxyribonuclease II beta
Short name=DNase II beta
Endonuclease DLAD

Gene names

Name:	Dnase2b
Synonyms:	Dlad

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes DNA under acidic conditions. Does not require divalent cations for activity. Participates in the degradation of nuclear DNA during lens cell differentiation. Ref.1
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.
Subcellular location	Lysosome Probable.
Tissue specificity	Liver specific. Ref.1
Miscellaneous	Inhibited by aurintricarboxylic acid and Zn2+.
Sequence similarities	Belongs to the DNase II family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	deoxyribonuclease II activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 356	334	Deoxyribonuclease-2-beta		PRO_0000007297
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	273	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9QZK9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5CBC259F42DD6741	FASTA	356	40,472
	10 20 30 40 50 60 MTAQPLKAAL PLLFVALSGV LGTPVISCIN EDGKAVDWFA FYKLPRRTSR GGTGMGLDYL 70 80 90 100 110 120 YLDSTMRTWS KSHHLINSSR SSLGRTLEQL YEAHNAKNDT AYLIYNDAVP ASVNYSGNYG 130 140 150 160 170 180 HAKGLLVWNR VQGFWLIHSI PKFPPVPEKG YEYPSSGRQY AQSGLCITLK YSQYETIDSQ 190 200 210 220 230 240 LLVFQPNIYS CFIPNIFRWE LIHMPQMCAK SSASKIPSRR LTVLQSAQGL NFLHFAKSTF 250 260 270 280 290 300 YTDDIFAAWI AQKLKVHLLV ESWQRKNHEL PSNCSLPYHV YNIKAIRGPL QSDFPSHHDH 310 320 330 340 350 SKWCVSTKDS QARWTCIGDL NRSPHQALRS GGFICSKNRY IYQSFDRLVS HYASCN

References

[1]	"Cloning of a cDNA encoding a rat DNase II-like acid DNase." Tanuma S., Shiokawa D. Biochem. Biophys. Res. Commun. 265:395-399(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Wistar. Tissue: Liver.
[2]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 48-71 AND 211-219, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF178974 mRNA. Translation: AAF13596.1.
IPI	IPI00215104.
PIR	JC7131.
RefSeq	NP_067696.1. NM_021664.1.
UniGene	Rn.41640.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000021861.
Proteomic databases
PRIDE	Q9QZK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	59296.
KEGG	rno:59296.
Organism-specific databases
CTD	58511.
RGD	70901. Dnase2b.
Phylogenomic databases
eggNOG	NOG145330.
HOGENOM	HOG000261682.
HOVERGEN	HBG051387.
InParanoid	Q9QZK9.
KO	K01158.
OrthoDB	EOG4MGS7K.
Gene expression databases
Genevestigator	Q9QZK9.
GermOnline	ENSRNOG00000016262. Rattus norvegicus.
Family and domain databases
InterPro	IPR004947. DNase_II. [Graphical view]
PANTHER	PTHR10858. PTHR10858. 1 hit.
Pfam	PF03265. DNase_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611825.

Entry information

Entry name

DNS2B_RAT

Accession

Primary (citable) accession number: Q9QZK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program