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Protein

Deoxyribonuclease-2-alpha

Gene

Dnase2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei298 – 2981By similarity

GO - Molecular functioni

  • deoxyribonuclease II activity Source: GO_Central
  • endodeoxyribonuclease activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BRENDAi3.1.22.1. 5301.
ReactomeiR-RNO-432720. Lysosome Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-2-alpha (EC:3.1.22.1)
Alternative name(s):
Acid DNase
Deoxyribonuclease II alpha
Short name:
DNase II alpha
Lysosomal DNase II
Gene namesi
Name:Dnase2
Synonyms:Dnase2a, Dnl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi621457. Dnase2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 350331Deoxyribonuclease-2-alphaPRO_0000007294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi22 ↔ 162Sequence analysis
Glycosylationi89 – 891N-linked (GlcNAc...)By similarity
Glycosylationi215 – 2151N-linked (GlcNAc...)By similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)By similarity
Disulfide bondi270 ↔ 350Sequence analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)By similarity
Disulfide bondi311 ↔ 330Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9QZK8.
PRIDEiQ9QZK8.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSRNOG00000023830.
GenevisibleiQ9QZK8. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014887.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase II family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
GeneTreeiENSGT00390000002634.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiQ9QZK8.
KOiK01158.
OMAiLQVQFWQ.
OrthoDBiEOG091G08N7.
PhylomeDBiQ9QZK8.
TreeFamiTF314536.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSSLLLA ALLWVPAEAL SCYGDSGRPV DWFVVYKLPA NSGSGDKPWK
60 70 80 90 100
GLMYKYMDQN SEGWQDGVGH IDSKDGAVGL TLQPLYQKNS SQLAFLLYND
110 120 130 140 150
QPPKSSSAQD SSSRGHTKGV LLLDQEGGFW LVHSVPRFPS PASSGAYSWP
160 170 180 190 200
PNARTYGQTL LCVSLPFSQF PGIGKQLTYT YPLVYDHKLE GIFAQKLPDL
210 220 230 240 250
EEVTKGHHVL REPWNSSVIL TSRAGTTFQS FAKFGKFGDD LYSGWLAAAL
260 270 280 290 300
GTNLQVQFWP NSPGILPSNC SGTHKILDVT ETGFPGPSGP TFNATEDHSK
310 320 330 340 350
WCVAPEGPWV CVGDMNRNKR ETHRGGGTLC TQVPALWKAF RSLVKACKPC
Length:350
Mass (Da):38,175
Last modified:May 1, 2000 - v1
Checksum:i6483AF5AC13C5111
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178975 mRNA. Translation: AAF13597.1.
BC091124 mRNA. Translation: AAH91124.1.
RefSeqiNP_612548.1. NM_138539.2.
UniGeneiRn.9509.

Genome annotation databases

EnsembliENSRNOT00000014887; ENSRNOP00000014887; ENSRNOG00000023830.
GeneIDi171575.
KEGGirno:171575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178975 mRNA. Translation: AAF13597.1.
BC091124 mRNA. Translation: AAH91124.1.
RefSeqiNP_612548.1. NM_138539.2.
UniGeneiRn.9509.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014887.

Proteomic databases

PaxDbiQ9QZK8.
PRIDEiQ9QZK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014887; ENSRNOP00000014887; ENSRNOG00000023830.
GeneIDi171575.
KEGGirno:171575.

Organism-specific databases

CTDi1777.
RGDi621457. Dnase2.

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
GeneTreeiENSGT00390000002634.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiQ9QZK8.
KOiK01158.
OMAiLQVQFWQ.
OrthoDBiEOG091G08N7.
PhylomeDBiQ9QZK8.
TreeFamiTF314536.

Enzyme and pathway databases

BRENDAi3.1.22.1. 5301.
ReactomeiR-RNO-432720. Lysosome Vesicle Biogenesis.

Miscellaneous databases

PROiQ9QZK8.

Gene expression databases

BgeeiENSRNOG00000023830.
GenevisibleiQ9QZK8. RN.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNS2A_RAT
AccessioniPrimary (citable) accession number: Q9QZK8
Secondary accession number(s): Q5BKC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Not required for the generation of the characteristic DNA fragmentation observed in apoptotic cells, but for the degradation of DNA from dying cells.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.