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Protein

Docking protein 3

Gene

Dok3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function.1 Publication

GO - Biological processi

  • Ras protein signal transduction Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Docking protein 3
Alternative name(s):
Downstream of tyrosine kinase 3
p62(dok)-like protein
Short name:
DOK-L
Gene namesi
Name:Dok3
Synonyms:Dokl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1351490. Dok3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi209R → A: Greatly reduced ABL1-binding; reduced tyrosine phosphorylation. 1 Publication1
Mutagenesisi224R → A: Greatly reduced ABL1-binding; reduced tyrosine phosphorylation. 1 Publication1
Mutagenesisi325Y → F: Enhanced reduction of BCR-induced IL2 secretion. No effect on interaction with INPP5D SH2 domain. Abolishes interaction with CSK SH2 domain. No effect on BCR-triggered tyrosine phosphorylation. Abolishes reduction of BCR-induced IL2 secretion, the interaction with INPP5D SH2 domain and BCR-triggered tyrosine phosphorylation; when associated with F-343. 1 Publication1
Mutagenesisi343Y → F: Some reduction of BCR-induced IL2 secretion. Some interaction with INPP5D SH2 domain. Some decrease in BCR-triggered tyrosine phosphorylation. Abolishes reduction of BCR-induced IL2 secretion, the interaction with INPP5D SH2 domain and BCR-triggered tyrosine phosphorylation; when associated with F-325. 1 Publication1
Mutagenesisi378Y → F: No effect on the reduction of BCR-induced IL2 secretion, nor on the interaction with the SH2 domain of INPP5D or CSK nor on BCR-triggered tyrosine phosphorylation; when associated with F-399. 1 Publication1
Mutagenesisi399Y → F: No effect on the reduction of BCR-induced IL2 secretion, nor on the interaction with the SH2 domain of INPP5D or CSK nor on BCR-triggered tyrosine phosphorylation; when associated with F-378. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872731 – 444Docking protein 3Add BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138PhosphoserineBy similarity1
Modified residuei274PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei325PhosphotyrosineCombined sources1
Modified residuei371PhosphoserineCombined sources1

Post-translational modificationi

Constitutively tyrosine-phosphorylated.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QZK7.
PaxDbiQ9QZK7.
PRIDEiQ9QZK7.

PTM databases

iPTMnetiQ9QZK7.
PhosphoSitePlusiQ9QZK7.

Expressioni

Tissue specificityi

Predominantly expressed in bone marrow, spleen and lung. Low levels in heart, brain, liver, muscle, thymus, kidney and testis. Highly expressed in B-cells and macrophages.2 Publications

Gene expression databases

BgeeiENSMUSG00000035711.
CleanExiMM_DOK3.
ExpressionAtlasiQ9QZK7. baseline and differential.
GenevisibleiQ9QZK7. MM.

Interactioni

Subunit structurei

On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-325 and Tyr-343 are required for interaction with INPP5D. Only Tyr-325 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
gagP033323EBI-2906753,EBI-935477From a different organism.

Protein-protein interaction databases

BioGridi205149. 7 interactors.
IntActiQ9QZK7. 4 interactors.
MINTiMINT-2491135.
STRINGi10090.ENSMUSP00000046695.

Structurei

3D structure databases

ProteinModelPortaliQ9QZK7.
SMRiQ9QZK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 123PHAdd BLAST117
Domaini157 – 261IRS-type PTBPROSITE-ProRule annotationAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi238 – 281Pro-richAdd BLAST44
Compositional biasi289 – 320Pro-richAdd BLAST32

Domaini

PTB domain mediates receptor interaction.By similarity

Sequence similaritiesi

Belongs to the DOK family. Type A subfamily.Curated
Contains 1 IRS-type PTB domain.PROSITE-ProRule annotation
Contains 1 PH domain.Curated

Phylogenomic databases

eggNOGiKOG4047. Eukaryota.
ENOG410XS2S. LUCA.
GeneTreeiENSGT00730000110348.
HOGENOMiHOG000112245.
HOVERGENiHBG051391.
InParanoidiQ9QZK7.
OMAiKGPAPCD.
OrthoDBiEOG091G075T.
PhylomeDBiQ9QZK7.
TreeFamiTF324994.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR002404. IRS_PTB.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF02174. IRS. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS51064. IRS_PTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q9QZK7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESVEPPVKD GILYQQHVKF GKKCWRKVWA LLYAGGPSGV ARLESWDVRD
60 70 80 90 100
GGLGPAGDRS TGPSRRGERR VIRLADCVSV LPADGESCPR DTGAFLITTT
110 120 130 140 150
ERSHLLAAQH RQSWVDPICQ LAFPGTGECS SGSGQAESPK RGFVPMEENS
160 170 180 190 200
IYSSWQEVTE FPVIVQRTEA TSRCQLKGPY LLVLGQDDIQ LRETSKPQAC
210 220 230 240 250
FSWPYRFLRK YGSDKGVFSF EAGRRCDSGE GLFAFSSPRA PDICGVVAAA
260 270 280 290 300
IARQRERLPE LAMSPPCPLP RALSLPSLEP PGELREVAPG FELPTPRKLP
310 320 330 340 350
LTDPGPQSLP LLLSPTQEGP ASGLYASVCK QTSKHTGTAE HLYENVCMLE
360 370 380 390 400
ASPGLTNGGP EAQEGPPGGR SPLGSPIYHN TEDLSWPGSA QDSNLEAQYR
410 420 430 440
RLLELELDEA GSAGRSGAQA GIKAKLVTLL TRERKKGPAP CDRP
Note: Major, and shortest isoform.
Length:444
Mass (Da):48,027
Last modified:May 1, 2000 - v1
Checksum:i124B705712202D9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179242 mRNA. Translation: AAF14285.1.
AF237580 mRNA. Translation: AAF61309.1.
CCDSiCCDS26548.1. [Q9QZK7-1]
RefSeqiNP_038767.1. NM_013739.2. [Q9QZK7-1]
XP_017171013.1. XM_017315524.1. [Q9QZK7-1]
UniGeneiMm.33910.

Genome annotation databases

EnsembliENSMUST00000047877; ENSMUSP00000046695; ENSMUSG00000035711. [Q9QZK7-1]
GeneIDi27261.
KEGGimmu:27261.
UCSCiuc007qrm.1. mouse. [Q9QZK7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179242 mRNA. Translation: AAF14285.1.
AF237580 mRNA. Translation: AAF61309.1.
CCDSiCCDS26548.1. [Q9QZK7-1]
RefSeqiNP_038767.1. NM_013739.2. [Q9QZK7-1]
XP_017171013.1. XM_017315524.1. [Q9QZK7-1]
UniGeneiMm.33910.

3D structure databases

ProteinModelPortaliQ9QZK7.
SMRiQ9QZK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205149. 7 interactors.
IntActiQ9QZK7. 4 interactors.
MINTiMINT-2491135.
STRINGi10090.ENSMUSP00000046695.

PTM databases

iPTMnetiQ9QZK7.
PhosphoSitePlusiQ9QZK7.

Proteomic databases

MaxQBiQ9QZK7.
PaxDbiQ9QZK7.
PRIDEiQ9QZK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047877; ENSMUSP00000046695; ENSMUSG00000035711. [Q9QZK7-1]
GeneIDi27261.
KEGGimmu:27261.
UCSCiuc007qrm.1. mouse. [Q9QZK7-1]

Organism-specific databases

CTDi79930.
MGIiMGI:1351490. Dok3.

Phylogenomic databases

eggNOGiKOG4047. Eukaryota.
ENOG410XS2S. LUCA.
GeneTreeiENSGT00730000110348.
HOGENOMiHOG000112245.
HOVERGENiHBG051391.
InParanoidiQ9QZK7.
OMAiKGPAPCD.
OrthoDBiEOG091G075T.
PhylomeDBiQ9QZK7.
TreeFamiTF324994.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9QZK7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035711.
CleanExiMM_DOK3.
ExpressionAtlasiQ9QZK7. baseline and differential.
GenevisibleiQ9QZK7. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR002404. IRS_PTB.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF02174. IRS. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS51064. IRS_PTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOK3_MOUSE
AccessioniPrimary (citable) accession number: Q9QZK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression of DOK3 inhibits the transforming activity of v-ABL, the ABL oncogene.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.