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Protein

Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial

Gene

Ecsit

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development.2 Publications
Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.By similarity

GO - Molecular functioni

  1. oxidoreductase activity, acting on NAD(P)H Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: MGI
  3. signal transducer activity Source: MGI

GO - Biological processi

  1. BMP signaling pathway Source: MGI
  2. innate immune response Source: UniProtKB-KW
  3. mesoderm formation Source: MGI
  4. oxidation-reduction process Source: MGI
  5. regulation of oxidoreductase activity Source: UniProtKB
  6. regulation of transcription from RNA polymerase II promoter Source: MGI
  7. transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Alternative name(s):
Protein SITPEC
Gene namesi
Name:Ecsit
Synonyms:Sitpec
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1349469. Ecsit.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication. Mitochondrion By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. mitochondrion Source: UniProtKB
  3. nucleoplasm Source: MGI
  4. nucleus Source: UniProtKB
  5. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848MitochondrionSequence AnalysisAdd
BLAST
Chaini49 – 435387Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrialPRO_0000291987Add
BLAST

Proteomic databases

MaxQBiQ9QZH6.
PaxDbiQ9QZH6.
PRIDEiQ9QZH6.

PTM databases

PhosphoSiteiQ9QZH6.

Expressioni

Tissue specificityi

Detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Detected in embryonic mesoderm and epiblast, and in extraembryonic ectoderm.2 Publications

Gene expression databases

BgeeiQ9QZH6.
CleanExiMM_ECSIT.
ExpressionAtlasiQ9QZH6. baseline and differential.
GenevestigatoriQ9QZH6.

Interactioni

Subunit structurei

Interacts with NDUFAF1 (By similarity). Interacts with ACAD9 (By similarity). Part of the mitochondrial complex I assembly (MCIA) complex. The complex comprises at least TMEM126B, NDUFAF1, ECSIT, and ACAD9 (By similarity). Interacts with MAP3K1, SMAD4 and TRAF6. Interacts with SMAD1 only after BMP4-treatment. Interacts with TRIM59 (PubMed:22588174).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRAF6Q9Y4K32EBI-527020,EBI-359276From a different organism.
Traf6P701964EBI-527020,EBI-448028

Protein-protein interaction databases

BioGridi205076. 5 interactions.
DIPiDIP-33901N.
IntActiQ9QZH6. 3 interactions.

Family & Domainsi

Sequence similaritiesi

Belongs to the ECSIT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG260596.
HOGENOMiHOG000112324.
HOVERGENiHBG059532.
InParanoidiQ9QZH6.
KOiK04405.
OrthoDBiEOG76T9RM.
PhylomeDBiQ9QZH6.
TreeFamiTF314943.

Family and domain databases

InterProiIPR029342. ECIST_C.
IPR010418. ECSIT.
[Graphical view]
PANTHERiPTHR13113. PTHR13113. 1 hit.
PfamiPF14784. ECIST_Cterm. 1 hit.
PF06239. ECSIT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWVQVNLLV RSLSRGWGGL CRPALSGTPF AQVSLQALRG LHCSAATHKD
60 70 80 90 100
EPWLVPRPPE PQRKPIKVPA MHEDLFKPSG NRERDKASFL NAVRSFGAHN
110 120 130 140 150
VRKRGHVDFI YLALRKMPEF GVERDLSVYN LLLDVFPKEV FRPRNVIQRI
160 170 180 190 200
FVHYPRQQEC GVAVLEQMER HGVMPSAETE FLLIQIFGRK SYPMLKFLRM
210 220 230 240 250
KLWFTRFKNI NPYPVPRDLP QDPLDLAKLG LRHMEPDLSA KVTVYQMSLP
260 270 280 290 300
SDSTGMEDPT QPHIVGIQSP DQQAALARHN PSRPVFVEGP FPLWLRNKCV
310 320 330 340 350
YYHILRADLP PPEEEKVEEI PEEWELYYPQ KLDLEYSRSG WDDYEFDVDE
360 370 380 390 400
VTEGPVFAMC MAGAHDQATL IKWIQGLQET NPTLAQIPVV FRLARSTGEL
410 420 430
LTTSRLEGQS PPHSPPKGPE EDDETIQAEQ QQGQS
Length:435
Mass (Da):49,799
Last modified:June 25, 2007 - v2
Checksum:iA2ABA68F1E2C6BF3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751L → S in AAF01219 (PubMed:10465784).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182510 mRNA. Translation: AAF01219.1.
BC004583 mRNA. Translation: AAH04583.1.
BC016424 mRNA. Translation: AAH16424.1.
CCDSiCCDS22920.1.
RefSeqiNP_001240826.1. NM_001253897.1.
NP_001240827.1. NM_001253898.1.
NP_036159.1. NM_012029.2.
XP_006510433.1. XM_006510370.2.
UniGeneiMm.425568.

Genome annotation databases

GeneIDi26940.
KEGGimmu:26940.
UCSCiuc009ons.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182510 mRNA. Translation: AAF01219.1.
BC004583 mRNA. Translation: AAH04583.1.
BC016424 mRNA. Translation: AAH16424.1.
CCDSiCCDS22920.1.
RefSeqiNP_001240826.1. NM_001253897.1.
NP_001240827.1. NM_001253898.1.
NP_036159.1. NM_012029.2.
XP_006510433.1. XM_006510370.2.
UniGeneiMm.425568.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205076. 5 interactions.
DIPiDIP-33901N.
IntActiQ9QZH6. 3 interactions.

PTM databases

PhosphoSiteiQ9QZH6.

Proteomic databases

MaxQBiQ9QZH6.
PaxDbiQ9QZH6.
PRIDEiQ9QZH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi26940.
KEGGimmu:26940.
UCSCiuc009ons.2. mouse.

Organism-specific databases

CTDi51295.
MGIiMGI:1349469. Ecsit.

Phylogenomic databases

eggNOGiNOG260596.
HOGENOMiHOG000112324.
HOVERGENiHBG059532.
InParanoidiQ9QZH6.
KOiK04405.
OrthoDBiEOG76T9RM.
PhylomeDBiQ9QZH6.
TreeFamiTF314943.

Miscellaneous databases

NextBioi304857.
PROiQ9QZH6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QZH6.
CleanExiMM_ECSIT.
ExpressionAtlasiQ9QZH6. baseline and differential.
GenevestigatoriQ9QZH6.

Family and domain databases

InterProiIPR029342. ECIST_C.
IPR010418. ECSIT.
[Graphical view]
PANTHERiPTHR13113. PTHR13113. 1 hit.
PfamiPF14784. ECIST_Cterm. 1 hit.
PF06239. ECSIT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway."
    Kopp E., Medzhitov R., Carothers J., Xiao C., Douglas I., Janeway C.A., Ghosh S.
    Genes Dev. 13:2059-2071(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TRAF6 AND MAP3K1, FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  3. "Ecsit is required for Bmp signaling and mesoderm formation during mouse embryogenesis."
    Xiao C., Shim J.-H., Klueppel M., Zhang S.S.-M., Dong C., Flavell R.A., Fu X.-Y., Wrana J.L., Hogan B.L.M., Ghosh S.
    Genes Dev. 17:2933-2949(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMAD1 AND SMAD4, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  4. "TRIM59 interacts with ECSIT and negatively regulates NF-kappaB and IRF-3/7-mediated signal pathways."
    Kondo T., Watanabe M., Hatakeyama S.
    Biochem. Biophys. Res. Commun. 422:501-507(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIM59.

Entry informationi

Entry nameiECSIT_MOUSE
AccessioniPrimary (citable) accession number: Q9QZH6
Secondary accession number(s): Q91V53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 25, 2007
Last sequence update: June 25, 2007
Last modified: March 31, 2015
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.