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Q9QZH6 (ECSIT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Alternative name(s):
Protein SITPEC
Gene names
Name:Ecsit
Synonyms:Sitpec
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. Ref.1 Ref.3

Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase By similarity. Ref.1 Ref.3

Subunit structure

Interacts with NDUFAF1 By similarity. Part of the mitochondrial complex I assembly (MCIA) complex. The complex comprises at least TMEM126B, NDUFAF1, ECSIT, and ACAD9 By similarity. Interacts with MAP3K1, SMAD4 and TRAF6. Interacts with SMAD1 only after BMP4-treatment. Ref.1 Ref.3

Subcellular location

Cytoplasm. Nucleus. Mitochondrion By similarity Ref.3.

Tissue specificity

Detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Detected in embryonic mesoderm and epiblast, and in extraembryonic ectoderm. Ref.1 Ref.3

Sequence similarities

Belongs to the ECSIT family.

Ontologies

Keywords
   Biological processImmunity
Innate immunity
   Cellular componentCytoplasm
Mitochondrion
Nucleus
   DomainTransit peptide
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from direct assay Ref.3. Source: MGI

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

mesoderm formation

Inferred from mutant phenotype Ref.3. Source: MGI

oxidation-reduction process

Inferred from sequence or structural similarity. Source: GOC

regulation of oxidoreductase activity

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.3. Source: MGI

transmembrane receptor protein serine/threonine kinase signaling pathway

Inferred from direct assay Ref.1. Source: MGI

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrion

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

transcription factor complex

Inferred from direct assay Ref.3. Source: MGI

   Molecular_functionoxidoreductase activity, acting on NAD(P)H

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.3. Source: MGI

signal transducer activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TRAF6Q9Y4K32EBI-527020,EBI-359276From a different organism.
Traf6P701964EBI-527020,EBI-448028

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4848Mitochondrion Potential
Chain49 – 435387Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
PRO_0000291987

Experimental info

Sequence conflict751L → S in AAF01219. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9QZH6 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: A2ABA68F1E2C6BF3

FASTA43549,799
        10         20         30         40         50         60 
MSWVQVNLLV RSLSRGWGGL CRPALSGTPF AQVSLQALRG LHCSAATHKD EPWLVPRPPE 

        70         80         90        100        110        120 
PQRKPIKVPA MHEDLFKPSG NRERDKASFL NAVRSFGAHN VRKRGHVDFI YLALRKMPEF 

       130        140        150        160        170        180 
GVERDLSVYN LLLDVFPKEV FRPRNVIQRI FVHYPRQQEC GVAVLEQMER HGVMPSAETE 

       190        200        210        220        230        240 
FLLIQIFGRK SYPMLKFLRM KLWFTRFKNI NPYPVPRDLP QDPLDLAKLG LRHMEPDLSA 

       250        260        270        280        290        300 
KVTVYQMSLP SDSTGMEDPT QPHIVGIQSP DQQAALARHN PSRPVFVEGP FPLWLRNKCV 

       310        320        330        340        350        360 
YYHILRADLP PPEEEKVEEI PEEWELYYPQ KLDLEYSRSG WDDYEFDVDE VTEGPVFAMC 

       370        380        390        400        410        420 
MAGAHDQATL IKWIQGLQET NPTLAQIPVV FRLARSTGEL LTTSRLEGQS PPHSPPKGPE 

       430 
EDDETIQAEQ QQGQS 

« Hide

References

« Hide 'large scale' references
[1]"ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway."
Kopp E., Medzhitov R., Carothers J., Xiao C., Douglas I., Janeway C.A., Ghosh S.
Genes Dev. 13:2059-2071(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TRAF6 AND MAP3K1, FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II and FVB/N.
Tissue: Mammary tumor.
[3]"Ecsit is required for Bmp signaling and mesoderm formation during mouse embryogenesis."
Xiao C., Shim J.-H., Klueppel M., Zhang S.S.-M., Dong C., Flavell R.A., Fu X.-Y., Wrana J.L., Hogan B.L.M., Ghosh S.
Genes Dev. 17:2933-2949(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMAD1 AND SMAD4, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF182510 mRNA. Translation: AAF01219.1.
BC004583 mRNA. Translation: AAH04583.1.
BC016424 mRNA. Translation: AAH16424.1.
RefSeqNP_001240826.1. NM_001253897.1.
NP_001240827.1. NM_001253898.1.
NP_036159.1. NM_012029.2.
XP_006510432.1. XM_006510369.1.
XP_006510433.1. XM_006510370.1.
UniGeneMm.425568.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid205076. 5 interactions.
DIPDIP-33901N.
IntActQ9QZH6. 3 interactions.

PTM databases

PhosphoSiteQ9QZH6.

Proteomic databases

PaxDbQ9QZH6.
PRIDEQ9QZH6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000098937; ENSMUSP00000096537; ENSMUSG00000066839.
ENSMUST00000179422; ENSMUSP00000137424; ENSMUSG00000066839.
ENSMUST00000180180; ENSMUSP00000136247; ENSMUSG00000066839.
GeneID26940.
KEGGmmu:26940.
UCSCuc009ons.2. mouse.

Organism-specific databases

CTD51295.
MGIMGI:1349469. Ecsit.

Phylogenomic databases

eggNOGNOG260596.
GeneTreeENSGT00390000005147.
HOGENOMHOG000112324.
HOVERGENHBG059532.
InParanoidQ9QZH6.
KOK04405.
OrthoDBEOG76T9RM.
PhylomeDBQ9QZH6.
TreeFamTF314943.

Gene expression databases

ArrayExpressQ9QZH6.
BgeeQ9QZH6.
CleanExMM_ECSIT.
GenevestigatorQ9QZH6.

Family and domain databases

InterProIPR010418. ECSIT.
[Graphical view]
PANTHERPTHR13113. PTHR13113. 1 hit.
PfamPF06239. ECSIT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio304857.
PROQ9QZH6.
SOURCESearch...

Entry information

Entry nameECSIT_MOUSE
AccessionPrimary (citable) accession number: Q9QZH6
Secondary accession number(s): Q91V53
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: April 16, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot