Q9QZH4 (AAKB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-AMP-activated protein kinase subunit beta-2
Short name=AMPK subunit beta-2

Gene names

Name:

Prkab2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) By similarity.
Subunit structure	AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3) By similarity.
Post-translational modification	Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Ref.1 Ref.2
Sequence similarities	Belongs to the 5'-AMP-activated protein kinase beta subunit family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	AMP-activated protein kinase complex Inferred from direct assay PubMed 16648175. Source: RGD apical plasma membrane Inferred from direct assay PubMed 18256313. Source: UniProtKB cAMP-dependent protein kinase complex Inferred from direct assay Ref.1. Source: RGD cytoplasm Inferred from direct assay PubMed 18256313. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	5'-AMP-activated protein kinase subunit beta-2		PRO_0000204370
Amino acid modifications
	Modified residue	38	1	Phosphoserine; by ULK1 Ref.2
	Modified residue	39	1	Phosphothreonine; by ULK1 Ref.2
	Modified residue	68	1	Phosphoserine; by ULK1 Ref.2
	Modified residue	107	1	Phosphoserine By similarity
	Modified residue	173	1	Phosphoserine; by ULK1 Ref.2
	Modified residue	183	1	Phosphoserine Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9QZH4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D881451A03287BEA	FASTA	271	30,227
	10 20 30 40 50 60 MGNTTSERVS GERHGAKAAR AEGGGHGPGK EHKIMVGSTD DPSVFSLPDS KLPGDKEFVP 70 80 90 100 110 120 WQQDLDDSVK PTQQARPTVI RWSEGGKEVF ISGSFNNWST KIPLIKSHND FVAILDLPEG 130 140 150 160 170 180 EHQYKFFVDG QWVHDPSEPV VTSQLGTINN LIHVKKSDFE VFDALKLDSM ESSETSCRDL 190 200 210 220 230 240 SSSPPGPYGQ EMYVFRSEER FKSPPILPPH LLQVILNKDT NISCDPALLP EPNHVMLNHL 250 260 270 YALSTKDSVM VLSATHRYKK KYVTTLLYKP I

References

[1]	"Expression of the AMP-activated protein kinase beta1 and beta2 subunits in skeletal muscle." Chen Z., Heierhorst J., Mann R.J., Mitchelhill K.I., Michell B.J., Witters L.A., Lynch G.S., Kemp B.E., Stapleton D. FEBS Lett. 460:343-348(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-183. Strain: Sprague-Dawley. Tissue: Skeletal muscle.
[2]	"Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop." Loffler A.S., Alers S., Dieterle A.M., Keppeler H., Franz-Wachtel M., Kundu M., Campbell D.G., Wesselborg S., Alessi D.R., Stork B. Autophagy 7:696-706(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY ULK1 AND ULK2, PHOSPHORYLATION AT SER-38; THR-39; SER-68 AND SER-173.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF182717 mRNA. Translation: AAF01293.1.
IPI	IPI00214997.
RefSeq	NP_072149.1. NM_022627.2.
UniGene	Rn.207202.
3D structure databases
ProteinModelPortal	Q9QZH4.
SMR	Q9QZH4. Positions 74-162, 189-271.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48.
PTM databases
PhosphoSite	Q9QZH4.
Protocols and materials databases
DNASU	64562.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	64562.
KEGG	rno:64562.
Organism-specific databases
CTD	5565.
RGD	620905. Prkab2.
Phylogenomic databases
eggNOG	NOG238368.
HOGENOM	HOG000230597.
HOVERGEN	HBG050430.
InParanoid	Q9QZH4.
KO	K07199.
Enzyme and pathway databases
Reactome	REACT_113568. Metabolism.
Gene expression databases
ArrayExpress	Q9QZH4.
Genevestigator	Q9QZH4.
GermOnline	ENSRNOG00000018166. Rattus norvegicus.
Family and domain databases
InterPro	IPR006828. AMP_prot_kin_bsu_interact-dom. [Graphical view]
Pfam	PF04739. AMPKBI. 1 hit. [Graphical view]
SMART	SM01010. AMPKBI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613490.

Entry information

Entry name

AAKB2_RAT

Accession

Primary (citable) accession number: Q9QZH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program