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Protein

Peptidyl-prolyl cis-trans isomerase E

Gene

Ppie

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.By similarity

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, RNA-binding, Rotamase
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6798695 Neutrophil degranulation
R-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase E (EC:5.2.1.8By similarity)
Short name:
PPIase E
Alternative name(s):
Cyclophilin E
Cyclophilin-33
Rotamase E
Gene namesi
Name:Ppie
Synonyms:Cyp331 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917118 Ppie

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000641581 – 301Peptidyl-prolyl cis-trans isomerase EAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei91PhosphoserineBy similarity1
Modified residuei97PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9QZH3
MaxQBiQ9QZH3
PaxDbiQ9QZH3
PeptideAtlasiQ9QZH3
PRIDEiQ9QZH3

2D gel databases

REPRODUCTION-2DPAGEiIPI00111343

PTM databases

iPTMnetiQ9QZH3
PhosphoSitePlusiQ9QZH3

Expressioni

Gene expression databases

BgeeiENSMUSG00000028651
CleanExiMM_PPIE
GenevisibleiQ9QZH3 MM

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19. Interacts (via RNA-binding domain) with KMT2A (via the third PHD-type zinc-finger).By similarity

Protein-protein interaction databases

BioGridi207784, 1 interactor
STRINGi10090.ENSMUSP00000030404

Structurei

3D structure databases

ProteinModelPortaliQ9QZH3
SMRiQ9QZH3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 83RRMPROSITE-ProRule annotationAdd BLAST79
Domaini143 – 299PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST157

Domaini

The RRM domain mediates both interaction with RNA and with KMT2A (via the third PHD-type zinc-finger), but has much higher affinity for the KMT2A PHD-type zinc-finger.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0111 Eukaryota
COG0652 LUCA
COG0724 LUCA
GeneTreeiENSGT00760000119119
HOGENOMiHOG000065981
HOVERGENiHBG001065
InParanoidiQ9QZH3
KOiK09564
OMAiFNLKHNS
OrthoDBiEOG091G0BGL
PhylomeDBiQ9QZH3
TreeFamiTF313817

Family and domain databases

CDDicd12347 RRM_PPIE, 1 hit
Gene3Di2.40.100.10, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR029000 Cyclophilin-like_dom_sf
IPR024936 Cyclophilin-type_PPIase
IPR020892 Cyclophilin-type_PPIase_CS
IPR002130 Cyclophilin-type_PPIase_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR016304 PPIE
IPR034168 PPIE_RRM
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR11071 PTHR11071, 1 hit
PfamiView protein in Pfam
PF00160 Pro_isomerase, 1 hit
PF00076 RRM_1, 1 hit
PIRSFiPIRSF001475 PPI_cyclophilin_E, 1 hit
PRINTSiPR00153 CSAPPISMRASE
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF50891 SSF50891, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS00170 CSA_PPIASE_1, 1 hit
PS50072 CSA_PPIASE_2, 1 hit
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QZH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA
60 70 80 90 100
FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKEG SSRPVWSDDD
110 120 130 140 150
WLKKFSGKTL EENKEEEGPE PPKAEAQEGE PTAKKARSNP QVYMDIKIGN
160 170 180 190 200
KPAGRIQMLL RSDVVPMTAE NFRCLCTHEK GFGFKGSSFH RIIPQFMCQG
210 220 230 240 250
GDFTNHNGTG GKSIYGKKFD DENFILKHTG PGLLSMANSG PNTNGSQFFL
260 270 280 290 300
TCDKTDWLDG KHVVFGEVTE GLDVLRQIEA QGSKDGKPKQ KVMIADCGEY

M
Length:301
Mass (Da):33,449
Last modified:September 26, 2003 - v2
Checksum:i856F7AE7F721B219
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62A → P in AAF01030 (Ref. 3) Curated1
Sequence conflicti241P → S in AAF01030 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008177 mRNA Translation: BAB25512.1
BC045154 mRNA Translation: AAH45154.1
AF182825 mRNA Translation: AAF01030.1
CCDSiCCDS18610.1
RefSeqiNP_062362.1, NM_019489.5
UniGeneiMm.126873

Genome annotation databases

EnsembliENSMUST00000030404; ENSMUSP00000030404; ENSMUSG00000028651
GeneIDi56031
KEGGimmu:56031
UCSCiuc008uow.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPPIE_MOUSE
AccessioniPrimary (citable) accession number: Q9QZH3
Secondary accession number(s): Q9D8C0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: September 26, 2003
Last modified: April 25, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health