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Q9QZH0 (FZD4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Frizzled-4

Short name=Fz-4
Short name=rFz4
Alternative name(s):
CD_antigen=CD344
Gene names
Name:Fzd4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length538 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues By similarity.

Subunit structure

Interacts with MAGI3 and NDP. Component of a complex, at least composed of TSPAN12, FZD4 and norrin (NDP) By similarity.

Subcellular location

Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein By similarity.

Domain

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity.

The FZ domain is involved in binding with Wnt ligands By similarity.

Post-translational modification

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
G-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway, calcium modulating pathway

Inferred from electronic annotation. Source: Ensembl

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

canonical Wnt signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

cerebellum vasculature morphogenesis

Inferred from electronic annotation. Source: Ensembl

extracellular matrix-cell signaling

Inferred from electronic annotation. Source: Ensembl

locomotion involved in locomotory behavior

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell-substrate adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of JUN kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

progesterone secretion

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of vascular endothelial growth factor receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

retinal blood vessel morphogenesis

Inferred from electronic annotation. Source: Ensembl

sensory perception of sound

Inferred from electronic annotation. Source: Ensembl

substrate adhesion-dependent cell spreading

Inferred from Biological aspect of Ancestor. Source: RefGenome

vasculogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentcell projection

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell surface

Inferred from electronic annotation. Source: Ensembl

cell-cell junction

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

PDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from physical interaction PubMed 18302287. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3737 Potential
Chain38 – 538501Frizzled-4
PRO_0000012987

Regions

Topological domain38 – 223186Extracellular Potential
Transmembrane224 – 24421Helical; Name=1; Potential
Topological domain245 – 25511Cytoplasmic Potential
Transmembrane256 – 27621Helical; Name=2; Potential
Topological domain277 – 30327Extracellular Potential
Transmembrane304 – 32421Helical; Name=3; Potential
Topological domain325 – 34521Cytoplasmic Potential
Transmembrane346 – 36621Helical; Name=4; Potential
Topological domain367 – 39024Extracellular Potential
Transmembrane391 – 41121Helical; Name=5; Potential
Topological domain412 – 43726Cytoplasmic Potential
Transmembrane438 – 45821Helical; Name=6; Potential
Topological domain459 – 47820Extracellular Potential
Transmembrane479 – 49921Helical; Name=7; Potential
Topological domain500 – 53839Cytoplasmic Potential
Domain41 – 162122FZ
Motif500 – 5056Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity
Motif536 – 5383PDZ-binding

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation3821N-linked (GlcNAc...) Potential
Disulfide bond46 ↔ 107 By similarity
Disulfide bond54 ↔ 100 By similarity
Disulfide bond91 ↔ 129 By similarity
Disulfide bond118 ↔ 159 By similarity
Disulfide bond122 ↔ 146 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QZH0 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 07DAAE606B46E005

FASTA53860,355
        10         20         30         40         50         60 
MAWQGTGPSV RGMPGGVRLR LGLLLLQLLL LQRPALGFGD EEERRCDPIR IAMCQNLGYN 

        70         80         90        100        110        120 
VTKMPNLVGH ELQTDAELQL TTFTPLIQYG CSSQLQFFLC SVYVPMCTEK INIPIGPCGG 

       130        140        150        160        170        180 
MCLSVKRRCE PVLKEFGFAW PDSLNCSKFP PQNDHNHMCM EGPGDEEVPL PHKTPIQPGE 

       190        200        210        220        230        240 
ECHSVGTNSD QYIWVKRSLN CVLKCGYDAG LYSRSAKEFT DIWMAVWASL CFISTTFTVL 

       250        260        270        280        290        300 
TFLIDSSRFS YPERPIIFLS MCYNIYSIAY IVRLTVGRER ISCDFEEAAE PVLIQEGLKN 

       310        320        330        340        350        360 
TGCAIIFLLM YFFGMASSIW WVILTLTWFL AAGLKWGHEA IEMHSSYFHI AAWAIPAVKT 

       370        380        390        400        410        420 
IVILIMRLVD ADELTGLCYV GNQSLDALTG FVVAPLFTYL VIGTLFIAAG LVALFKIRSN 

       430        440        450        460        470        480 
LQKDGTKTDK LERLMVKIGV FSVLYTVPAT CVIACYFYEI SNWALFRYSA DDSNMAVEML 

       490        500        510        520        530 
KIFMSLLVGI TSGMWIWSAK TLHTWQKCSN RLVNSGKVKR EKRGNGWVKP GKGNETVV 

« Hide

References

[1]"Expression of a putative seven-pass transmembrane frizzled receptor in the Corpus Luteum (CL) of the rat during pregnancy and involution."
Walther P.R., Lacher M.D., Saurer S., Friis R.R.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Corpus luteum.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF183910 mRNA. Translation: AAF01036.1.
RefSeqNP_072145.1. NM_022623.2.
UniGeneRn.18388.
Rn.48736.

3D structure databases

ProteinModelPortalQ9QZH0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023205.

Protein family/group databases

MEROPSI93.001.
GPCRDBSearch...

PTM databases

PhosphoSiteQ9QZH0.

Proteomic databases

PaxDbQ9QZH0.
PRIDEQ9QZH0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023205; ENSRNOP00000023205; ENSRNOG00000016848.
GeneID64558.
KEGGrno:64558.
UCSCRGD:71017. rat.

Organism-specific databases

CTD8322.
RGD71017. Fzd4.

Phylogenomic databases

eggNOGNOG284049.
GeneTreeENSGT00750000117488.
HOGENOMHOG000233236.
HOVERGENHBG006977.
InParanoidQ9QZH0.
KOK02354.
OMASMCLSVK.
OrthoDBEOG7M3J01.
PhylomeDBQ9QZH0.
TreeFamTF317907.

Gene expression databases

GenevestigatorQ9QZH0.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026551. FZD4.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF23. PTHR11309:SF23. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613472.
PROQ9QZH0.

Entry information

Entry nameFZD4_RAT
AccessionPrimary (citable) accession number: Q9QZH0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries