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Protein

DNA repair endonuclease XPF

Gene

Ercc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link (By similarity).By similarity

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMagnesium

Enzyme and pathway databases

ReactomeiR-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6783310 Fanconi Anemia Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair endonuclease XPF (EC:3.1.-.-)
Alternative name(s):
DNA excision repair protein ERCC-4
Gene namesi
Name:Ercc4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1354163 Ercc4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988541 – 917DNA repair endonuclease XPFAdd BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei289N6-acetyllysineBy similarity1
Modified residuei522PhosphoserineCombined sources1
Modified residuei765PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9QZD4
MaxQBiQ9QZD4
PaxDbiQ9QZD4
PeptideAtlasiQ9QZD4
PRIDEiQ9QZD4

PTM databases

iPTMnetiQ9QZD4
PhosphoSitePlusiQ9QZD4

Expressioni

Gene expression databases

BgeeiENSMUSG00000022545
ExpressionAtlasiQ9QZD4 baseline and differential
GenevisibleiQ9QZD4 MM

Interactioni

Subunit structurei

Heterodimer composed of ERCC1 and XPF/ERCC4. Interacts with SLX4/BTBD12; this interaction is direct and links the ERCC1-XPF/ERCC1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206047, 3 interactors
STRINGi10090.ENSMUSP00000023206

Structurei

3D structure databases

ProteinModelPortaliQ9QZD4
SMRiQ9QZD4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini684 – 764ERCC4Add BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 457Helicase-likeBy similarityAdd BLAST457
Regioni233 – 254Leucine-zipper 1Add BLAST22
Regioni270 – 298Leucine-zipper 2Add BLAST29
Regioni659 – 814NucleaseBy similarityAdd BLAST156
Regioni838 – 906HhH2, dimerization with ERCC1By similarityAdd BLAST69

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi487 – 492Nuclear localization signalSequence analysis6

Sequence similaritiesi

Belongs to the XPF family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0442 Eukaryota
COG1948 LUCA
GeneTreeiENSGT00390000004394
HOGENOMiHOG000202204
HOVERGENiHBG051500
InParanoidiQ9QZD4
KOiK10848
OMAiRNVRVYF
OrthoDBiEOG091G0554
PhylomeDBiQ9QZD4
TreeFamiTF101234

Family and domain databases

InterProiView protein in InterPro
IPR006166 ERCC4_domain
IPR011335 Restrct_endonuc-II-like
IPR010994 RuvA_2-like
IPR006167 XPF
PfamiView protein in Pfam
PF02732 ERCC4, 1 hit
SMARTiView protein in SMART
SM00891 ERCC4, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF52980 SSF52980, 1 hit
TIGRFAMsiTIGR00596 rad1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QZD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGLSGERR SMAPLLEYER QQVLELLDSD GLVVCARGLG TDRLLYHFLR
60 70 80 90 100
LHCHPACLVL VLNTQPAEEE YFINQLKIEG VEHLPRRVTN EIASNSRYEV
110 120 130 140 150
YTQGGIIFAT SRILVVDFLT GRIPSDLITG ILVYRAHRII ESCQEAFILR
160 170 180 190 200
LFRQKNKRGF IKAFTDNAVA FDTGFCHVER VMRNLFVRKL YLWPRFHVAV
210 220 230 240 250
NSFLEQHKPE VVEIHVSMTP AMLAIQTAIL DILNACLKEL KCHNPSLEVE
260 270 280 290 300
DLSLENALGK PFDKTIRHYL DPLWHQLGAK TKSLVQDLKI LRTLLQYLSQ
310 320 330 340 350
YDCVTFLNLL ESLRATEKVF GQNSGWLFLD ASTSMFVNAR ARVYRVPDVK
360 370 380 390 400
LNKKAKTSEK TSSPEVQETK KELVLESNPK WEALTDVLKE IEAENKESEA
410 420 430 440 450
LGGPGRVLIC ASDDRTCCQL RDYLSAGAET FLLRLYRKTF EKDGKAEEVW
460 470 480 490 500
VNVRKGDGPK RTTKSDKRPK AAPNKERASA KRGAPLKRKK QELTLTQVLG
510 520 530 540 550
SAEEPPEDKA LEEDLCRQTS SSPEGCGVEI KRESFDLNVS SDAAYGILKE
560 570 580 590 600
PLTIIHPLLG CSDPYALTRV LHEVEPRYVV LYDAELTFVR QLEIYRASRP
610 620 630 640 650
GKPLRVYFLI YGGSTEEQRY LTALRKEKEA FEKLIREKAS MVVPEEREGR
660 670 680 690 700
DETNLDLARG SAALDAPTDT RKAGGQEQNG TQSSIVVDMR EFRSELPSLI
710 720 730 740 750
HRRGIDIEPV TLEVGDYILT PELCVERKSV SDLIGSLHSG RLYSQCLAMS
760 770 780 790 800
RYYRRPVLLI EFDPSKPFSL APRGAFFQEM SSSDVSSKLT LLTLHFPRLR
810 820 830 840 850
LLWCPSPHAT AELFEELKQN KPQPDAATAM AITADSETLP ESDRYNPGPQ
860 870 880 890 900
DFVLKMPGVN AKNCRSLMNQ VKNIAELATL SLERLTTILG HSGNAKQLHD
910
FLHTAYADLV SKGRVRK
Length:917
Mass (Da):103,690
Last modified:May 5, 2009 - v3
Checksum:i2206DC264BC4E233
GO

Sequence cautioni

The sequence AAH26792 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30D → N in AAF03157 (PubMed:10644440).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF189285 mRNA Translation: AAF03157.1
AC004155 Genomic DNA Translation: AAC03240.1
BC026792 mRNA Translation: AAH26792.1 Different initiation.
CCDSiCCDS37256.1
RefSeqiNP_056584.2, NM_015769.2
UniGeneiMm.287837

Genome annotation databases

EnsembliENSMUST00000023206; ENSMUSP00000023206; ENSMUSG00000022545
GeneIDi50505
KEGGimmu:50505
UCSCiuc007yfx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiXPF_MOUSE
AccessioniPrimary (citable) accession number: Q9QZD4
Secondary accession number(s): O54810, Q8R0I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 5, 2009
Last modified: May 23, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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