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Protein

Growth factor receptor-bound protein 7

Gene

Grb7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei238Important for lipid binding and for stimulation of cell migrationBy similarity1
Sitei514Important for dimerization and for HRAS activationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, RNA-binding
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-1306955. GRB7 events in ERBB2 signaling.
R-RNO-1433557. Signaling by SCF-KIT.
R-RNO-186763. Downstream signal transduction.
R-RNO-210993. Tie2 Signaling.
R-RNO-8853659. RET signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth factor receptor-bound protein 7
Alternative name(s):
Epidermal growth factor receptor GRB-7
GRB7 adapter protein
Gene namesi
Name:Grb7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi619759. Grb7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi461R → K: Reduces interaction with INSR. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004122101 – 535Growth factor receptor-bound protein 7Add BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187Phosphotyrosine; by FAK1By similarity1
Modified residuei341Phosphotyrosine; by FAK1By similarity1
Modified residuei364PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to activation of receptor kinases. Phosphorylated on tyrosine residues by TEK/TIE2. Phosphorylated on tyrosine residues by PTK2/FAK1, and possibly also other kinases. Phosphorylation is enhanced by activation of receptor kinases by a cognate ligand. Tyrosine phosphorylation is essential for activation of down-stream protein kinases. Phosphorylation decreases affinity for target mRNA molecules (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QZC5.
PRIDEiQ9QZC5.

PTM databases

iPTMnetiQ9QZC5.
PhosphoSitePlusiQ9QZC5.

Expressioni

Tissue specificityi

Detected in liver, kidney and placenta, and at lower levels in lung.1 Publication

Gene expression databases

BgeeiENSRNOG00000006990.
GenevisibleiQ9QZC5. RN.

Interactioni

Subunit structurei

Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1 (By similarity). Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009299.

Structurei

3D structure databases

ProteinModelPortaliQ9QZC5.
SMRiQ9QZC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 185Ras-associatingPROSITE-ProRule annotationAdd BLAST87
Domaini228 – 341PHPROSITE-ProRule annotationAdd BLAST114
Domaini434 – 530SH2PROSITE-ProRule annotationAdd BLAST97

Domaini

The PH domain mediates interaction with membranes containing phosphoinositides.By similarity

Sequence similaritiesi

Belongs to the GRB7/10/14 family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG3751. Eukaryota.
ENOG410XXC8. LUCA.
GeneTreeiENSGT00550000074537.
HOGENOMiHOG000231904.
HOVERGENiHBG000468.
InParanoidiQ9QZC5.
OMAiLSDENWG.
OrthoDBiEOG091G135G.
PhylomeDBiQ9QZC5.
TreeFamiTF317511.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR015042. BPS-dom.
IPR035032. Grb7.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
PANTHERiPTHR11243:SF35. PTHR11243:SF35. 1 hit.
PfamiView protein in Pfam
PF08947. BPS. 1 hit.
PF00169. PH. 1 hit.
PF00788. RA. 1 hit.
PF00017. SH2. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiView protein in PROSITE
PS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9QZC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDLSPSHL SSSPEDVCPT PGTPPETPPP PDNPPPGDVK RSQPLPIPSS
60 70 80 90 100
RKLREEEFQA TSLPSIPNPF PELCSPPSQK PILGGSSGAR GLLPRDSSRL
110 120 130 140 150
CVVKVYSEDG ACRSVEVAAG ATARHVCEML VQRAHALSDE NWGLVECHPY
160 170 180 190 200
LALERGLEDH ESVVEVQEAW PVGGDSRFIF RKNFAKYELF KSPPHTLFPE
210 220 230 240 250
KMVSSCLDTP TGISHEDLIQ NFLNAGSFPE IQGFLQLRGS GRGSGRKLWK
260 270 280 290 300
RFFCFLRRSG LYYSTKGTSK DPRHLQYVAD INESNVYVVT QGRKLYGIPT
310 320 330 340 350
DFGFCVKPNK LRNGHKGLHI FCSEDEQSRT CWLSAFRLFK YGVQLYKNYQ
360 370 380 390 400
QAQSRHLRLS YLGSPPLRSV SDNTLVAMDF SGHAGRVIEN PQEALSAATE
410 420 430 440 450
EAQAWRKKTN HRLSLPTPCS GLSLSAAIHR TQPWFHGRIS REESQRLIGQ
460 470 480 490 500
QGLVDGVFLV RESQRNPQGF VLSLCHLQKV KHYLILPSED EGCLYFSMDD
510 520 530
GQTRFTDLLQ LVEFHQLNRG ILPCLLRHCC ARVAL
Length:535
Mass (Da):59,889
Last modified:May 1, 2000 - v1
Checksum:i15DB67C4D19B89E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190121 mRNA. Translation: AAF01776.1.
CH473948 Genomic DNA. Translation: EDM05933.1.
BC081757 mRNA. Translation: AAH81757.1.
RefSeqiNP_445855.1. NM_053403.2.
XP_006247494.1. XM_006247432.3.
XP_006247495.1. XM_006247433.3.
XP_008766347.1. XM_008768125.1.
XP_017453048.1. XM_017597559.1.
XP_017453049.1. XM_017597560.1.
XP_017453050.1. XM_017597561.1.
UniGeneiRn.28109.

Genome annotation databases

EnsembliENSRNOT00000009299; ENSRNOP00000009299; ENSRNOG00000006990.
GeneIDi84427.
KEGGirno:84427.
UCSCiRGD:619759. rat.

Similar proteinsi

Entry informationi

Entry nameiGRB7_RAT
AccessioniPrimary (citable) accession number: Q9QZC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families