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Protein

Programmed cell death 6-interacting protein

Gene

Pdcd6ip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (By similarity). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).By similarity

GO - Molecular functioni

  • SH3 domain binding Source: RGD

GO - Biological processi

Keywordsi

Biological processApoptosis, Cell cycle, Cell division, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 6-interacting protein
Alternative name(s):
ALG-2-interacting protein 1
Gene namesi
Name:Pdcd6ip
Synonyms:Aip1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68357. Pdcd6ip.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication
  • Melanosome By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Secretedexosome By similarity
  • Cell junctiontight junction By similarity
  • MidbodyMidbody ring By similarity

  • Note: Colocalized with CEP55 in the midbody during cytokinesis and at centrosomes in non-dividing cells (By similarity). Component of the actomyosin-tight junction complex (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Secreted, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003492962 – 873Programmed cell death 6-interacting proteinAdd BLAST872

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei215N6-acetyllysineBy similarity1
Modified residuei484PhosphothreonineBy similarity1
Modified residuei486PhosphoserineBy similarity1
Modified residuei735PhosphoserineBy similarity1
Modified residuei742PhosphothreonineBy similarity1
Modified residuei745PhosphothreonineBy similarity1
Modified residuei749Omega-N-methylarginineBy similarity1

Post-translational modificationi

May be phosphorylated on tyrosine residues by activated PDGFRB.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QZA2.
PRIDEiQ9QZA2.

2D gel databases

World-2DPAGEi0004:Q9QZA2.

PTM databases

iPTMnetiQ9QZA2.
PhosphoSitePlusiQ9QZA2.

Expressioni

Tissue specificityi

Expressed in astrocytes and glioma cells.

Gene expression databases

BgeeiENSRNOG00000008981.
GenevisibleiQ9QZA2. RN.

Interactioni

Subunit structurei

Self-associates (By similarity). Interacts with SH3KBP1 (PubMed:10858458). Interacts with PDCD6 in a calcium-dependent manner (By similarity). Interacts with TSG101 in a calcium-dependent manner; PDCD6IP homooligomerization may be required for TSG101-binding (By similarity). Interacts with SGSM3 (By similarity). Directly interacts with CHMP4A, CHMP4B and CHMP4C (By similarity). Directly interacts with CEP55 in a 1:2 stoechiometry; this interaction is required for PDCD6IP targeting to the midbody (By similarity). May interact with PDGFRB (By similarity). Interacts with SH3GL1 and SH3GL2/endophilin-1 (By similarity). Forms a complex with SDCBP and SDC2 (By similarity). Found in a complex with F-actin, TJP1/ZO-1 and PARD3 (By similarity). Interacts with CD2AP (By similarity).By similarity1 Publication

GO - Molecular functioni

  • SH3 domain binding Source: RGD

Protein-protein interaction databases

BioGridi272390. 1 interactor.
MINTiMINT-4567363.
STRINGi10116.ENSRNOP00000012114.

Structurei

3D structure databases

SMRiQ9QZA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 397BRO1PROSITE-ProRule annotationAdd BLAST395

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 508Interaction with CHMP4A, CHMP4B and CHMP4CBy similarityAdd BLAST333
Regioni423 – 873Interaction with SDCBPBy similarityAdd BLAST451
Regioni508 – 873Self-associationBy similarityAdd BLAST366
Regioni722 – 725Interaction with TSG101By similarity4
Regioni802 – 811Interaction with CEP55By similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi722 – 865Pro-richAdd BLAST144

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
HOGENOMiHOG000006938.
HOVERGENiHBG053533.
InParanoidiQ9QZA2.
OrthoDBiEOG091G04NC.
PhylomeDBiQ9QZA2.

Family and domain databases

InterProiView protein in InterPro
IPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
PfamiView protein in Pfam
PF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
SMARTiView protein in SMART
SM01041. BRO1. 1 hit.
PROSITEiView protein in PROSITE
PS51180. BRO1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR
60 70 80 90 100
RSALGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD
110 120 130 140 150
KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA
160 170 180 190 200
KQYQFASGAF LHIKDTVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF
210 220 230 240 250
LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDALPKY FYFQEVFPTL
260 270 280 290 300
AAKQCIMQAN AEYHQSILAK QQKKFGEEIA RLQHAAELIK NVASRYDEYV
310 320 330 340 350
NVKDFSDKIN RALAAAKKDN DFIYHDRVPD LKDLDPIGKA TLVKPTPVNV
360 370 380 390 400
PISQKFTDLF EKMVPVSVQQ SLAVFSQRKA DLVNRSIAQM REATTLANGV
410 420 430 440 450
LASLNLPAAI EDVSGDTVPQ SILTKSTAVV EQGGIQTVDQ LIKELPELLQ
460 470 480 490 500
RNREILEESL RLLDEEEATD NDLRAKFKDR WQRTPSNDLY KPLRAEGAKF
510 520 530 540 550
RAVLDKAVQA DGQVKERYQS HRDTIALLCK PEPELNAAIP SANPAKTMQG
560 570 580 590 600
SEVVNVLKSL LSNLDEIKKE REGLENDLKS VNFDMTSKFL TALAQDGVIN
610 620 630 640 650
EEALSVTELD RIYGGLTTKV QESLKKQEGL LKNIQVSHQE FSKMKQSNSE
660 670 680 690 700
ASLREEVLKN LATAYDNFVE LVANLKEGTK FYNELTEILV RFQNKCSDIV
710 720 730 740 750
FARKTERDEL LKDLQQSIAR EPSAPSIPPP AYQSSPAGGH ATAPTPAPRT
760 770 780 790 800
MPPAKPQPPA RPPPPVLPAN RVPPAAAATA PAGVGTASAA PPQTPGSAPP
810 820 830 840 850
PQAQGPPYPT YPGYPGYCQM PMPMGYNPYT YGQYNMPYPP VYHQSPGQAP
860 870
YPGPQQPTYP FPQPPQQSYY PQQ
Length:873
Mass (Da):96,631
Last modified:September 2, 2008 - v2
Checksum:iFD7B5D57825D571D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03063785 Genomic DNA. No translation available.
AABR03064061 Genomic DNA. No translation available.
AF192757 mRNA. Translation: AAF07179.1.
RefSeqiXP_006244059.1. XM_006243997.2.
UniGeneiRn.101381.
Rn.1588.
Rn.226240.

Genome annotation databases

GeneIDi501083.
UCSCiRGD:68357. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPDC6I_RAT
AccessioniPrimary (citable) accession number: Q9QZA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: September 2, 2008
Last modified: July 5, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome