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Protein

Programmed cell death 6-interacting protein

Gene

Pdcd6ip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (By similarity).By similarity

GO - Molecular functioni

  • SH3 domain binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 6-interacting protein
Alternative name(s):
ALG-2-interacting protein 1
Gene namesi
Name:Pdcd6ip
Synonyms:Aip1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68357. Pdcd6ip.

Subcellular locationi

  • Melanosome By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Cytoplasmcytosol 1 Publication
  • Secretedexosome By similarity

  • Note: Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB-SubCell
  • melanosome Source: UniProtKB-SubCell
  • microtubule organizing center Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 873872Programmed cell death 6-interacting proteinPRO_0000349296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei215 – 2151N6-acetyllysineBy similarity
Modified residuei484 – 4841PhosphothreonineBy similarity
Modified residuei486 – 4861PhosphoserineBy similarity
Modified residuei735 – 7351PhosphoserineBy similarity
Modified residuei742 – 7421PhosphothreonineBy similarity
Modified residuei745 – 7451PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QZA2.
PRIDEiQ9QZA2.

2D gel databases

World-2DPAGE0004:Q9QZA2.

PTM databases

iPTMnetiQ9QZA2.
PhosphoSiteiQ9QZA2.

Expressioni

Tissue specificityi

Expressed in astrocytes and glioma cells.

Gene expression databases

BgeeiENSRNOG00000008981.
GenevisibleiQ9QZA2. RN.

Interactioni

Subunit structurei

Interacts with SH3KBP1 (PubMed:10858458). Interacts with PDCD6; the interaction is calcium-dependent (By similarity). Interacts with TSG101 (By similarity). Interacts with SGSM3. Self-associates. Interacts with CHMP4A; the interaction is direct. Interacts with CHMP4B; the interaction is direct. Interacts with CHMP4C; the interaction is direct. Interacts with CEP55; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoichiometry. Interacts with SH3GL1 and SH3GL2. Interacts with SDCBP. Forms a complex with SDCBP and SDC2 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • SH3 domain binding Source: RGD

Protein-protein interaction databases

BioGridi272390. 1 interaction.
MINTiMINT-4567363.
STRINGi10116.ENSRNOP00000012114.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 397395BRO1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 508333Interaction with CHMP4A, CHMP4B and CHMP4CBy similarityAdd
BLAST
Regioni423 – 873451Interaction with SDCBPBy similarityAdd
BLAST
Regioni508 – 873366Self-associationBy similarityAdd
BLAST
Regioni722 – 7254Interaction with TSG101By similarity
Regioni802 – 81110Interaction with CEP55By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi722 – 865144Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 BRO1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
HOGENOMiHOG000006938.
HOVERGENiHBG053533.
InParanoidiQ9QZA2.
OrthoDBiEOG091G04NC.
PhylomeDBiQ9QZA2.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR
60 70 80 90 100
RSALGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD
110 120 130 140 150
KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA
160 170 180 190 200
KQYQFASGAF LHIKDTVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF
210 220 230 240 250
LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDALPKY FYFQEVFPTL
260 270 280 290 300
AAKQCIMQAN AEYHQSILAK QQKKFGEEIA RLQHAAELIK NVASRYDEYV
310 320 330 340 350
NVKDFSDKIN RALAAAKKDN DFIYHDRVPD LKDLDPIGKA TLVKPTPVNV
360 370 380 390 400
PISQKFTDLF EKMVPVSVQQ SLAVFSQRKA DLVNRSIAQM REATTLANGV
410 420 430 440 450
LASLNLPAAI EDVSGDTVPQ SILTKSTAVV EQGGIQTVDQ LIKELPELLQ
460 470 480 490 500
RNREILEESL RLLDEEEATD NDLRAKFKDR WQRTPSNDLY KPLRAEGAKF
510 520 530 540 550
RAVLDKAVQA DGQVKERYQS HRDTIALLCK PEPELNAAIP SANPAKTMQG
560 570 580 590 600
SEVVNVLKSL LSNLDEIKKE REGLENDLKS VNFDMTSKFL TALAQDGVIN
610 620 630 640 650
EEALSVTELD RIYGGLTTKV QESLKKQEGL LKNIQVSHQE FSKMKQSNSE
660 670 680 690 700
ASLREEVLKN LATAYDNFVE LVANLKEGTK FYNELTEILV RFQNKCSDIV
710 720 730 740 750
FARKTERDEL LKDLQQSIAR EPSAPSIPPP AYQSSPAGGH ATAPTPAPRT
760 770 780 790 800
MPPAKPQPPA RPPPPVLPAN RVPPAAAATA PAGVGTASAA PPQTPGSAPP
810 820 830 840 850
PQAQGPPYPT YPGYPGYCQM PMPMGYNPYT YGQYNMPYPP VYHQSPGQAP
860 870
YPGPQQPTYP FPQPPQQSYY PQQ
Length:873
Mass (Da):96,631
Last modified:September 2, 2008 - v2
Checksum:iFD7B5D57825D571D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03063785 Genomic DNA. No translation available.
AABR03064061 Genomic DNA. No translation available.
AF192757 mRNA. Translation: AAF07179.1.
RefSeqiXP_006244059.1. XM_006243997.1.
UniGeneiRn.101381.
Rn.1588.
Rn.226240.

Genome annotation databases

GeneIDi501083.
UCSCiRGD:68357. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03063785 Genomic DNA. No translation available.
AABR03064061 Genomic DNA. No translation available.
AF192757 mRNA. Translation: AAF07179.1.
RefSeqiXP_006244059.1. XM_006243997.1.
UniGeneiRn.101381.
Rn.1588.
Rn.226240.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi272390. 1 interaction.
MINTiMINT-4567363.
STRINGi10116.ENSRNOP00000012114.

PTM databases

iPTMnetiQ9QZA2.
PhosphoSiteiQ9QZA2.

2D gel databases

World-2DPAGE0004:Q9QZA2.

Proteomic databases

PaxDbiQ9QZA2.
PRIDEiQ9QZA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi501083.
UCSCiRGD:68357. rat.

Organism-specific databases

CTDi10015.
RGDi68357. Pdcd6ip.

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
HOGENOMiHOG000006938.
HOVERGENiHBG053533.
InParanoidiQ9QZA2.
OrthoDBiEOG091G04NC.
PhylomeDBiQ9QZA2.

Miscellaneous databases

PROiQ9QZA2.

Gene expression databases

BgeeiENSRNOG00000008981.
GenevisibleiQ9QZA2. RN.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDC6I_RAT
AccessioniPrimary (citable) accession number: Q9QZA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: September 2, 2008
Last modified: September 7, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.