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Protein

Interferon-inducible GTPase 1

Gene

Iigp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase with low activity. Has higher affinity for GDP than for GTP. Plays a role in resistance to intracellular pathogens. Required for disruption of the parasitophorous vacuole formed following T.gondii infection and subsequent killing of the parasite. Mediates resistance to C.trachomatis infection by targeting bacterial inclusions to autophagosomes for subsequent lysosomal destruction.4 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84GTP1 Publication8
Nucleotide bindingi102 – 106GTP1 Publication5
Nucleotide bindingi184 – 186GTP1 Publication3
Nucleotide bindingi231 – 233GTP1 Publication3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • identical protein binding Source: MGI

GO - Biological processi

  • cellular response to interferon-beta Source: MGI
  • cytokine-mediated signaling pathway Source: MGI
  • defense response to Gram-negative bacterium Source: UniProtKB
  • defense response to protozoan Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • regulation of autophagy Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-inducible GTPase 1 (EC:3.6.5.-)
Gene namesi
Name:Iigp1
Synonyms:Irga6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1926259. Iigp1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • nuclear membrane Source: UniProtKB-SubCell
  • symbiont-containing vacuole membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Protein is detected exclusively in the aqueous phase. 1 Publication1
Mutagenesisi82K → A: Constitutively active. Binds GTP but fails to hydrolyze it. Does not localize to the parasitophorous vacuole membrane following T.gondii infection. 2 Publications1
Mutagenesisi83S → N: Abrogates interaction with HOOK3. Greatly reduces binding affinity for GDP and GTP. Abolishes GTP-dependent oligomer formation. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00002235272 – 413Interferon-inducible GTPase 1Add BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineCurated1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiQ9QZ85.
PaxDbiQ9QZ85.
PRIDEiQ9QZ85.

PTM databases

iPTMnetiQ9QZ85.
PhosphoSitePlusiQ9QZ85.

Expressioni

Inductioni

Up-regulated by IFNG, IFNA1 and lipopolysaccharide (LPS) within 20 hours. Transiently up-regulated during the early stages of infection by Listeria monocytogenes. After 6 days expression is back to basal levels.2 Publications

Gene expression databases

BgeeiENSMUSG00000054072.
CleanExiMM_IIGP1.
ExpressionAtlasiQ9QZ85. baseline and differential.
GenevisibleiQ9QZ85. MM.

Interactioni

Subunit structurei

Monomer, as apoenzyme and in the GDP-bound form. Homooligomer, upon GTP binding. Interacts with HOOK3.3 Publications

GO - Molecular functioni

  • identical protein binding Source: MGI

Protein-protein interaction databases

BioGridi208563. 2 interactors.
DIPiDIP-58958N.
IntActiQ9QZ85. 4 interactors.
MINTiMINT-1857405.
STRINGi10090.ENSMUSP00000032473.

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 25Combined sources10
Helixi29 – 31Combined sources3
Helixi36 – 47Combined sources12
Helixi51 – 66Combined sources16
Beta strandi70 – 75Combined sources6
Helixi82 – 90Combined sources9
Beta strandi94 – 96Combined sources3
Helixi104 – 108Combined sources5
Beta strandi112 – 115Combined sources4
Beta strandi122 – 126Combined sources5
Helixi130 – 132Combined sources3
Helixi137 – 143Combined sources7
Helixi146 – 148Combined sources3
Beta strandi150 – 159Combined sources10
Helixi162 – 173Combined sources12
Beta strandi179 – 183Combined sources5
Helixi185 – 195Combined sources11
Turni197 – 199Combined sources3
Helixi202 – 219Combined sources18
Beta strandi221 – 223Combined sources3
Beta strandi227 – 229Combined sources3
Turni233 – 236Combined sources4
Beta strandi237 – 239Combined sources3
Helixi240 – 249Combined sources10
Helixi253 – 255Combined sources3
Helixi256 – 262Combined sources7
Helixi268 – 288Combined sources21
Beta strandi292 – 294Combined sources3
Helixi296 – 298Combined sources3
Helixi303 – 319Combined sources17
Helixi324 – 333Combined sources10
Helixi338 – 342Combined sources5
Helixi347 – 349Combined sources3
Beta strandi355 – 357Combined sources3
Helixi359 – 374Combined sources16
Beta strandi377 – 379Combined sources3
Helixi386 – 409Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TPZX-ray2.00A/B1-411[»]
1TQ2X-ray2.70A/B1-411[»]
1TQ4X-ray1.95A1-413[»]
1TQ6X-ray2.70A1-413[»]
1TQDX-ray2.30A/B1-413[»]
4LV5X-ray1.70B1-413[»]
4LV8X-ray1.72B1-413[»]
5FPHX-ray3.20A/B/C/D/E/F/G1-413[»]
ProteinModelPortaliQ9QZ85.
SMRiQ9QZ85.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QZ85.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 250IRG-type GAdd BLAST183

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410J4GH. Eukaryota.
ENOG41125KF. LUCA.
GeneTreeiENSGT00390000014733.
HOGENOMiHOG000220822.
HOVERGENiHBG054304.
InParanoidiQ9QZ85.
OMAiVELNIAI.
OrthoDBiEOG091G0B2S.
PhylomeDBiQ9QZ85.
TreeFamiTF331897.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR030385. G_IRG_dom.
IPR007743. Immunity-related_GTPase-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF05049. IIGP. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51716. G_IRG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZ85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQLFSSPKS DENNDLPSSF TGYFKKFNTG RKIISQEILN LIELRMRKGN
60 70 80 90 100
IQLTNSAISD ALKEIDSSVL NVAVTGETGS GKSSFINTLR GIGNEEEGAA
110 120 130 140 150
KTGVVEVTME RHPYKHPNIP NVVFWDLPGI GSTNFPPNTY LEKMKFYEYD
160 170 180 190 200
FFIIISATRF KKNDIDIAKA ISMMKKEFYF VRTKVDSDIT NEADGKPQTF
210 220 230 240 250
DKEKVLQDIR LNCVNTFREN GIAEPPIFLL SNKNVCHYDF PVLMDKLISD
260 270 280 290 300
LPIYKRHNFM VSLPNITDSV IEKKRQFLKQ RIWLEGFAAD LVNIIPSLTF
310 320 330 340 350
LLDSDLETLK KSMKFYRTVF GVDETSLQRL ARDWEIEVDQ VEAMIKSPAV
360 370 380 390 400
FKPTDEETIQ ERLSRYIQEF CLANGYLLPK NSFLKEIFYL KYYFLDMVTE
410
DAKTLLKEIC LRN
Length:413
Mass (Da):47,572
Last modified:February 21, 2006 - v2
Checksum:i44B5A95FCA58A37A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138N → D in AAF07195 (PubMed:11907101).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007971 mRNA. Translation: CAA07798.1.
AF194871 mRNA. Translation: AAF07195.1.
AK153060 mRNA. Translation: BAE31686.1.
CT010300 mRNA. Translation: CAJ18508.1.
BC004649 mRNA. Translation: AAH04649.1.
CCDSiCCDS29270.1.
RefSeqiNP_001139747.1. NM_001146275.1.
NP_068564.4. NM_021792.4.
XP_011245279.1. XM_011246977.2.
XP_011245280.1. XM_011246978.2.
XP_011245281.1. XM_011246979.2.
XP_011245282.1. XM_011246980.1.
UniGeneiMm.261140.

Genome annotation databases

EnsembliENSMUST00000032473; ENSMUSP00000032473; ENSMUSG00000054072.
ENSMUST00000066912; ENSMUSP00000063390; ENSMUSG00000054072.
GeneIDi60440.
KEGGimmu:60440.
UCSCiuc008fac.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007971 mRNA. Translation: CAA07798.1.
AF194871 mRNA. Translation: AAF07195.1.
AK153060 mRNA. Translation: BAE31686.1.
CT010300 mRNA. Translation: CAJ18508.1.
BC004649 mRNA. Translation: AAH04649.1.
CCDSiCCDS29270.1.
RefSeqiNP_001139747.1. NM_001146275.1.
NP_068564.4. NM_021792.4.
XP_011245279.1. XM_011246977.2.
XP_011245280.1. XM_011246978.2.
XP_011245281.1. XM_011246979.2.
XP_011245282.1. XM_011246980.1.
UniGeneiMm.261140.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TPZX-ray2.00A/B1-411[»]
1TQ2X-ray2.70A/B1-411[»]
1TQ4X-ray1.95A1-413[»]
1TQ6X-ray2.70A1-413[»]
1TQDX-ray2.30A/B1-413[»]
4LV5X-ray1.70B1-413[»]
4LV8X-ray1.72B1-413[»]
5FPHX-ray3.20A/B/C/D/E/F/G1-413[»]
ProteinModelPortaliQ9QZ85.
SMRiQ9QZ85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208563. 2 interactors.
DIPiDIP-58958N.
IntActiQ9QZ85. 4 interactors.
MINTiMINT-1857405.
STRINGi10090.ENSMUSP00000032473.

PTM databases

iPTMnetiQ9QZ85.
PhosphoSitePlusiQ9QZ85.

Proteomic databases

EPDiQ9QZ85.
PaxDbiQ9QZ85.
PRIDEiQ9QZ85.

Protocols and materials databases

DNASUi60440.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032473; ENSMUSP00000032473; ENSMUSG00000054072.
ENSMUST00000066912; ENSMUSP00000063390; ENSMUSG00000054072.
GeneIDi60440.
KEGGimmu:60440.
UCSCiuc008fac.2. mouse.

Organism-specific databases

CTDi60440.
MGIiMGI:1926259. Iigp1.

Phylogenomic databases

eggNOGiENOG410J4GH. Eukaryota.
ENOG41125KF. LUCA.
GeneTreeiENSGT00390000014733.
HOGENOMiHOG000220822.
HOVERGENiHBG054304.
InParanoidiQ9QZ85.
OMAiVELNIAI.
OrthoDBiEOG091G0B2S.
PhylomeDBiQ9QZ85.
TreeFamiTF331897.

Miscellaneous databases

EvolutionaryTraceiQ9QZ85.
PROiQ9QZ85.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054072.
CleanExiMM_IIGP1.
ExpressionAtlasiQ9QZ85. baseline and differential.
GenevisibleiQ9QZ85. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR030385. G_IRG_dom.
IPR007743. Immunity-related_GTPase-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF05049. IIGP. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51716. G_IRG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIIGP1_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ85
Secondary accession number(s): Q9Z1M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 21, 2006
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.