Reviewed, UniProtKB/Swiss-Prot Q9QZ82 (CP11A_MOUSE)

Names and origin

Protein names

Recommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    Cytochrome P450 11A1
    CYPXIA1
    P450(scc)
    Cholesterol desmolase

Gene names

Name:	Cyp11a1
Synonyms:	Cyp11a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone By similarity.
Catalytic activity	Cholesterol + reduced adrenal ferredoxin + O2 = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from direct assay. Source: MGI cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cholesterol monooxygenase (side-chain-cleaving) activity Inferred from direct assay. Source: MGI electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Mitochondrion By similarity
	Chain	37 – 526	490	Cholesterol side-chain cleavage enzyme, mitochondrial		PRO_0000003587
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QZ82-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E5029738EE4ECB71	FASTA	526	60,315
	10 20 30 40 50 60 MLAKGLSLRS VLVKGCQPFL SPTWQGPVLS TGKGAGTSTS SPRSFNEIPS PGDNGWLNLY 70 80 90 100 110 120 HFWRESGTQK IHYHQMQSFQ KYGPIYREKL GTLESVYIVD PKDASILFSC EGPNPERFLV 130 140 150 160 170 180 PPWVAYHQYY QRPIGVLFKS SDAWKKDRIV LNQEVMAPGA IKNFVPLLEG VAQDFIKVLH 190 200 210 220 230 240 RRIKQQNSGN FSGVISDDLF RFSFESISSV IFGERMGMLE EIVDPEAQRF INAVYQMFHT 250 260 270 280 290 300 SVPMLNLPPD FFRLLRTKTW KDHAAAWDVI FNKADEYTQN FYWDLRQKRD FSQYPGVLYS 310 320 330 340 350 360 LLGGNKLPFK NIQANITEML AGGVDTTSMT LQWNLYEMAH NLKVQEMLRA EVLAARRQAQ 370 380 390 400 410 420 GDMAKMVQLV PLLKASIKET LRLHPISVTL QRYTVNDLVL RNYKIPAKTL VQVASFAMGR 430 440 450 460 470 480 DPGFFPNPNK FDPTRWLEKS QNTTHFRYLG FGWGVRQCLG RRIAELEMTI LLINLLENFR 490 500 510 520 IEVQNLRDVG TKFSLILMPE NPILFNFQPL KQDLGPAVTR KDNTVN

References

[1]	Tanaka M., Hennebold J.H., Adashi E.Y. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF195119 mRNA. Translation: AAF03897.1.
IPI	IPI00136928.
RefSeq	NP_062753.3.
UniGene	Mm.302865
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9QZ82.
Proteomic databases
PRIDE	Q9QZ82.
Genome annotation databases
Ensembl	ENSMUST00000034874; ENSMUSP00000034874; ENSMUSG00000032323; Mus musculus. [Genome view]
GeneID	13070.
KEGG	mmu:13070.
UCSC	uc009pwa.1. mouse.
Organism-specific databases
CTD	13070.
MGI	MGI:88582. Cyp11a1.
Phylogenomic databases
HOGENOM	Q9QZ82.
HOVERGEN	Q9QZ82.
OMA	NQEVMAP.
Enzyme and pathway databases
BRENDA	1.14.15.6. 244.
Gene expression databases
ArrayExpress	Q9QZ82.
Bgee	Q9QZ82.
Genevestigator	Q9QZ82.
GermOnline	ENSMUSG00000032323. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283004.
SOURCE	Search...

Entry information

Entry name

CP11A_MOUSE

Accession

Primary (citable) accession number: Q9QZ82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)