Q9QZ82 (CP11A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholesterol side-chain cleavage enzyme, mitochondrial
EC=1.14.15.6

Alternative name(s):
CYPXIA1
Cholesterol desmolase
Cytochrome P450 11A1
Cytochrome P450(scc)

Gene names

Name:	Cyp11a1
Synonyms:	Cyp11a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone By similarity.
Catalytic activity	Cholesterol + 6 reduced adrenodoxin + 3 O2 = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subunit structure	Interacts with FDX1/adrenodoxin By similarity.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis Sterol metabolism
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C21-steroid hormone biosynthetic process Inferred from direct assay PubMed 7527351. Source: MGI Leydig cell differentiation Inferred from electronic annotation. Source: Compara Schwann cell differentiation Inferred from electronic annotation. Source: Compara biphenyl metabolic process Inferred from electronic annotation. Source: Compara cellular response to antibiotic Inferred from electronic annotation. Source: Compara cellular response to cAMP Inferred from electronic annotation. Source: Compara cellular response to cadmium ion Inferred from electronic annotation. Source: Compara cellular response to fibroblast growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to follicle-stimulating hormone stimulus Inferred from electronic annotation. Source: Compara cellular response to interleukin-1 Inferred from electronic annotation. Source: Compara cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara cellular response to peptide hormone stimulus Inferred from electronic annotation. Source: Compara cellular response to transforming growth factor beta stimulus Inferred from electronic annotation. Source: Compara cellular response to tumor necrosis factor Inferred from electronic annotation. Source: Compara cerebellum development Inferred from electronic annotation. Source: Compara cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW dibenzo-p-dioxin metabolic process Inferred from electronic annotation. Source: Compara estrogen biosynthetic process Inferred from electronic annotation. Source: Compara fractalkine metabolic process Inferred from electronic annotation. Source: Compara granulosa cell differentiation Inferred from electronic annotation. Source: Compara hippocampus development Inferred from electronic annotation. Source: Compara maternal process involved in female pregnancy Inferred from electronic annotation. Source: Compara mating behavior Inferred from electronic annotation. Source: Compara phenol-containing compound metabolic process Inferred from electronic annotation. Source: Compara phthalate metabolic process Inferred from electronic annotation. Source: Compara progesterone biosynthetic process Inferred from electronic annotation. Source: Compara response to L-ascorbic acid Inferred from electronic annotation. Source: Compara response to acid Inferred from electronic annotation. Source: Compara response to alkaloid Inferred from electronic annotation. Source: Compara response to corticosterone stimulus Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to fungicide Inferred from electronic annotation. Source: Compara response to gamma radiation Inferred from electronic annotation. Source: Compara response to genistein Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from electronic annotation. Source: Compara response to insecticide Inferred from electronic annotation. Source: Compara response to salt stress Inferred from electronic annotation. Source: Compara response to vitamin E Inferred from electronic annotation. Source: Compara testosterone biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial crista Inferred from electronic annotation. Source: Compara mitochondrion Inferred from direct assay PubMed 14651853PubMed 18614015PubMed 8111631. Source: MGI perikaryon Inferred from electronic annotation. Source: Compara
Molecular_function	cholesterol binding Inferred from electronic annotation. Source: Compara cholesterol monooxygenase (side-chain-cleaving) activity Inferred from direct assay PubMed 7527351. Source: MGI electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Mitochondrion By similarity
	Chain	37 – 526	490	Cholesterol side-chain cleavage enzyme, mitochondrial		PRO_0000003587
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QZ82 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E5029738EE4ECB71	FASTA	526	60,315
	10 20 30 40 50 60 MLAKGLSLRS VLVKGCQPFL SPTWQGPVLS TGKGAGTSTS SPRSFNEIPS PGDNGWLNLY 70 80 90 100 110 120 HFWRESGTQK IHYHQMQSFQ KYGPIYREKL GTLESVYIVD PKDASILFSC EGPNPERFLV 130 140 150 160 170 180 PPWVAYHQYY QRPIGVLFKS SDAWKKDRIV LNQEVMAPGA IKNFVPLLEG VAQDFIKVLH 190 200 210 220 230 240 RRIKQQNSGN FSGVISDDLF RFSFESISSV IFGERMGMLE EIVDPEAQRF INAVYQMFHT 250 260 270 280 290 300 SVPMLNLPPD FFRLLRTKTW KDHAAAWDVI FNKADEYTQN FYWDLRQKRD FSQYPGVLYS 310 320 330 340 350 360 LLGGNKLPFK NIQANITEML AGGVDTTSMT LQWNLYEMAH NLKVQEMLRA EVLAARRQAQ 370 380 390 400 410 420 GDMAKMVQLV PLLKASIKET LRLHPISVTL QRYTVNDLVL RNYKIPAKTL VQVASFAMGR 430 440 450 460 470 480 DPGFFPNPNK FDPTRWLEKS QNTTHFRYLG FGWGVRQCLG RRIAELEMTI LLINLLENFR 490 500 510 520 IEVQNLRDVG TKFSLILMPE NPILFNFQPL KQDLGPAVTR KDNTVN

References

[1]	Tanaka M., Hennebold J.H., Adashi E.Y. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF195119 mRNA. Translation: AAF03897.1.
IPI	IPI00136928.
RefSeq	NP_062753.3. NM_019779.3.
UniGene	Mm.302865.
3D structure databases
ProteinModelPortal	Q9QZ82.
SMR	Q9QZ82. Positions 41-510.
ModBase	Search...
PTM databases
PhosphoSite	Q9QZ82.
Proteomic databases
PaxDb	Q9QZ82.
PRIDE	Q9QZ82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034874; ENSMUSP00000034874; ENSMUSG00000032323.
GeneID	13070.
KEGG	mmu:13070.
UCSC	uc009pwa.1. mouse.
Organism-specific databases
CTD	1583.
MGI	MGI:88582. Cyp11a1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00550000074304.
HOGENOM	HOG000013161.
HOVERGEN	HBG051098.
InParanoid	Q9QZ82.
KO	K00498.
OMA	RDYMIPA.
OrthoDB	EOG4GXFMF.
Enzyme and pathway databases
UniPathway	UPA00229.
Gene expression databases
Bgee	Q9QZ82.
Genevestigator	Q9QZ82.
GermOnline	ENSMUSG00000032323. Mus musculus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	283004.
SOURCE	Search...

Entry information

Entry name

CP11A_MOUSE

Accession

Primary (citable) accession number: Q9QZ82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program