Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DCN1-like protein 1

Gene

Dcun1d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic in vivo.1 Publication

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
DCN1-like protein 1
Alternative name(s):
DCUN1 domain-containing protein 1
Defective in cullin neddylation protein 1-like protein 1
Testis-specific protein 3
Gene namesi
Name:Dcun1d1
Synonyms:Dcun1l1, Rp42, Tes3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2150386. Dcun1d1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259DCN1-like protein 1PRO_0000129499Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9QZ73.
MaxQBiQ9QZ73.
PaxDbiQ9QZ73.
PRIDEiQ9QZ73.

PTM databases

iPTMnetiQ9QZ73.
PhosphoSiteiQ9QZ73.

Expressioni

Tissue specificityi

Highly expressed in testis. Also expressed in brain, heart, liver, skeletal muscle and kidney. In brain, preferentially expressed in the telencephalon ventricular and subventricular zones, albeit at low levels. In adult testis, expressed in cells above seminiferous tubules, but only weakly in spermatogonia.2 Publications

Developmental stagei

Expressed in the developing forebrain, midbrain and hindbrain at early stages of neuronal development.1 Publication

Gene expression databases

BgeeiQ9QZ73.
ExpressionAtlasiQ9QZ73. baseline and differential.
GenevisibleiQ9QZ73. MM.

Interactioni

Subunit structurei

Part of an E3 complex for neddylation composed of cullins, RBX1, UBE2M and CAND1. Interacts (via the C-terminus 50 AA) with CUL1, CUL2, CUL3, CUL4 and CUL5. Binds neddylated CUL1. Interacts (via the C-terminus 50 AA) directly with RBX1. Interacts preferentially with UBE2M-NEDD8 thioester (via N-terminus 1-26 AA) than with free UBE2M. UBE2M N-terminal acetylation increases the affinity of this interaction by about 2 orders of magnitude. Interacts with CAND1 when in complex with CUL1-RBX1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103817.

Structurei

3D structure databases

ProteinModelPortaliQ9QZ73.
SMRiQ9QZ73. Positions 6-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4538UBA-likeAdd
BLAST
Domaini60 – 248189DCUN1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DCUN1 domain.PROSITE-ProRule annotation
Contains 1 UBA-like domain.Curated

Phylogenomic databases

eggNOGiKOG3077. Eukaryota.
ENOG410XTIJ. LUCA.
GeneTreeiENSGT00550000074529.
HOGENOMiHOG000241761.
HOVERGENiHBG055256.
InParanoidiQ9QZ73.
KOiK17822.
OMAiEQTAIYC.
OrthoDBiEOG7S4X6Q.
PhylomeDBiQ9QZ73.

Family and domain databases

InterProiIPR014764. DCN-prot.
IPR005176. PONY_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR12281. PTHR12281. 1 hit.
PfamiPF03556. Cullin_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51229. DCUN1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QZ73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLKSSQKD KVRQFMIFTQ SSEKTAVSCL SQNDWKLDVA TDNFFQNPEL
60 70 80 90 100
YIRESVKGSL DRKKLEQLYT RYKDPQDENK IGIDGIQQFC DDLALDPASI
110 120 130 140 150
SVLIIAWKFR AATQCEFSKQ EFMDGMTELG CDSIEKLKAQ IPKMEQELKE
160 170 180 190 200
PGRFKDFYQF TFNFAKNPGQ KGLDLEMAIA YWNLVLNGRF KFLDLWNKFL
210 220 230 240 250
LEHHKRSIPK DTWNLLLDFS SMIADDMSNY DEEGAWPVLI DDFVEFARPQ

IAGTKSTTV
Length:259
Mass (Da):30,097
Last modified:May 1, 2000 - v1
Checksum:i06E095D5F58EF20A
GO

Sequence cautioni

The sequence BAC26390.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → S in BAC26390 (PubMed:16141072).Curated
Sequence conflicti115 – 1151C → F in BAC26390 (PubMed:16141072).Curated
Sequence conflicti259 – 2591V → L in BAC26390 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198092 mRNA. Translation: AAF04863.1.
AK008657 mRNA. Translation: BAB25813.1.
AK029314 mRNA. Translation: BAC26390.1. Different initiation.
AK155081 mRNA. Translation: BAE33033.1.
AK165651 mRNA. Translation: BAE38318.1.
BC020161 mRNA. Translation: AAH20161.1.
BC031666 mRNA. Translation: AAH31666.1.
BC037431 mRNA. Translation: AAH37431.1.
CCDSiCCDS57207.1.
RefSeqiNP_001192290.1. NM_001205361.1.
UniGeneiMm.374810.
Mm.379305.
Mm.441473.
Mm.445013.
Mm.474495.

Genome annotation databases

EnsembliENSMUST00000108182; ENSMUSP00000103817; ENSMUSG00000027708.
GeneIDi114893.
KEGGimmu:114893.
UCSCiuc008oyw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198092 mRNA. Translation: AAF04863.1.
AK008657 mRNA. Translation: BAB25813.1.
AK029314 mRNA. Translation: BAC26390.1. Different initiation.
AK155081 mRNA. Translation: BAE33033.1.
AK165651 mRNA. Translation: BAE38318.1.
BC020161 mRNA. Translation: AAH20161.1.
BC031666 mRNA. Translation: AAH31666.1.
BC037431 mRNA. Translation: AAH37431.1.
CCDSiCCDS57207.1.
RefSeqiNP_001192290.1. NM_001205361.1.
UniGeneiMm.374810.
Mm.379305.
Mm.441473.
Mm.445013.
Mm.474495.

3D structure databases

ProteinModelPortaliQ9QZ73.
SMRiQ9QZ73. Positions 6-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103817.

PTM databases

iPTMnetiQ9QZ73.
PhosphoSiteiQ9QZ73.

Proteomic databases

EPDiQ9QZ73.
MaxQBiQ9QZ73.
PaxDbiQ9QZ73.
PRIDEiQ9QZ73.

Protocols and materials databases

DNASUi114893.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108182; ENSMUSP00000103817; ENSMUSG00000027708.
GeneIDi114893.
KEGGimmu:114893.
UCSCiuc008oyw.2. mouse.

Organism-specific databases

CTDi54165.
MGIiMGI:2150386. Dcun1d1.

Phylogenomic databases

eggNOGiKOG3077. Eukaryota.
ENOG410XTIJ. LUCA.
GeneTreeiENSGT00550000074529.
HOGENOMiHOG000241761.
HOVERGENiHBG055256.
InParanoidiQ9QZ73.
KOiK17822.
OMAiEQTAIYC.
OrthoDBiEOG7S4X6Q.
PhylomeDBiQ9QZ73.

Miscellaneous databases

NextBioi368913.
PROiQ9QZ73.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QZ73.
ExpressionAtlasiQ9QZ73. baseline and differential.
GenevisibleiQ9QZ73. MM.

Family and domain databases

InterProiIPR014764. DCN-prot.
IPR005176. PONY_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR12281. PTHR12281. 1 hit.
PfamiPF03556. Cullin_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51229. DCUN1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new family of genes and pseudogenes potentially expressing testis-specific and brain-specific leucine-zipper proteins in man and mouse."
    Pourcel C., Jaubert J., Hadchouel M., Wu X., Schweizer J.
    Gene 249:105-113(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Cloning and expression analysis of a novel gene, RP42, mapping to an autism susceptibility locus on 6q16."
    Mas C., Bourgeois F., Bulfone A., Levacher B., Mugnier C., Simonneau M.
    Genomics 65:70-74(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Telencephalon.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Colon, Head and Stomach.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. Cited for: DEVELOPMENTAL STAGE, FUNCTION.

Entry informationi

Entry nameiDCNL1_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ73
Secondary accession number(s): Q3TMX2, Q8CDZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.