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Protein

DCN1-like protein 1

Gene

Dcun1d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (By similarity). Acts also as an oncogene facilitating malignant transformation and carcinogenic in vivo (PubMed:20563250).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei115Essential for interaction with UBE2MBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
DCN1-like protein 1
Alternative name(s):
DCUN1 domain-containing protein 1
Defective in cullin neddylation protein 1-like protein 1
Testis-specific protein 3
Gene namesi
Name:Dcun1d1
Synonyms:Dcun1l1, Rp42, Tes3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2150386 Dcun1d1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001294991 – 259DCN1-like protein 1Add BLAST259

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9QZ73
MaxQBiQ9QZ73
PaxDbiQ9QZ73
PRIDEiQ9QZ73

PTM databases

iPTMnetiQ9QZ73
PhosphoSitePlusiQ9QZ73

Expressioni

Tissue specificityi

Highly expressed in testis. Also expressed in brain, heart, liver, skeletal muscle and kidney. In brain, preferentially expressed in the telencephalon ventricular and subventricular zones, albeit at low levels. In adult testis, expressed in cells above seminiferous tubules, but only weakly in spermatogonia.2 Publications

Developmental stagei

Expressed in the developing forebrain, midbrain and hindbrain at early stages of neuronal development.1 Publication

Gene expression databases

BgeeiENSMUSG00000027708
ExpressionAtlasiQ9QZ73 baseline and differential
GenevisibleiQ9QZ73 MM

Interactioni

Subunit structurei

Part of an E3 complex for neddylation composed of cullins, RBX1, UBE2M and CAND1. Interacts (via the C-terminus 50 AA) with CUL1, CUL2, CUL3, CUL4 and CUL5. Binds neddylated CUL1. Interacts (via the C-terminus 50 AA) directly with RBX1. Interacts (via DCUN1 domain) with UBE2M (acetylated at N-terminal methionine). Interacts preferentially with UBE2M-NEDD8 thioester (via N-terminus 1-26 AA) than with free UBE2M. UBE2M N-terminal acetylation increases the affinity of this interaction by about 2 orders of magnitude. Interacts with CAND1 when in complex with CUL1-RBX1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103817

Structurei

3D structure databases

ProteinModelPortaliQ9QZ73
SMRiQ9QZ73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 45UBA-likeAdd BLAST38
Domaini60 – 248DCUN1PROSITE-ProRule annotationAdd BLAST189

Domaini

The DCUN1 domain, also known as PONY domain mediates its interaction with N-terminally acetylated UBE2M.By similarity

Phylogenomic databases

eggNOGiKOG3077 Eukaryota
ENOG410XTIJ LUCA
GeneTreeiENSGT00550000074529
HOGENOMiHOG000241761
HOVERGENiHBG055256
InParanoidiQ9QZ73
KOiK17822
OMAiMITDDMS
OrthoDBiEOG091G0HHH
PhylomeDBiQ9QZ73

Family and domain databases

InterProiView protein in InterPro
IPR014764 DCN-prot
IPR005176 PONY_dom
IPR009060 UBA-like_sf
PANTHERiPTHR12281 PTHR12281, 1 hit
PfamiView protein in Pfam
PF03556 Cullin_binding, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
PROSITEiView protein in PROSITE
PS51229 DCUN1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QZ73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLKSSQKD KVRQFMIFTQ SSEKTAVSCL SQNDWKLDVA TDNFFQNPEL
60 70 80 90 100
YIRESVKGSL DRKKLEQLYT RYKDPQDENK IGIDGIQQFC DDLALDPASI
110 120 130 140 150
SVLIIAWKFR AATQCEFSKQ EFMDGMTELG CDSIEKLKAQ IPKMEQELKE
160 170 180 190 200
PGRFKDFYQF TFNFAKNPGQ KGLDLEMAIA YWNLVLNGRF KFLDLWNKFL
210 220 230 240 250
LEHHKRSIPK DTWNLLLDFS SMIADDMSNY DEEGAWPVLI DDFVEFARPQ

IAGTKSTTV
Length:259
Mass (Da):30,097
Last modified:May 1, 2000 - v1
Checksum:i06E095D5F58EF20A
GO

Sequence cautioni

The sequence BAC26390 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13R → S in BAC26390 (PubMed:16141072).Curated1
Sequence conflicti115C → F in BAC26390 (PubMed:16141072).Curated1
Sequence conflicti259V → L in BAC26390 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198092 mRNA Translation: AAF04863.1
AK008657 mRNA Translation: BAB25813.1
AK029314 mRNA Translation: BAC26390.1 Different initiation.
AK155081 mRNA Translation: BAE33033.1
AK165651 mRNA Translation: BAE38318.1
BC020161 mRNA Translation: AAH20161.1
BC031666 mRNA Translation: AAH31666.1
BC037431 mRNA Translation: AAH37431.1
CCDSiCCDS57207.1
RefSeqiNP_001192290.1, NM_001205361.1
UniGeneiMm.374810
Mm.379305
Mm.441473
Mm.445013
Mm.474495

Genome annotation databases

EnsembliENSMUST00000108182; ENSMUSP00000103817; ENSMUSG00000027708
GeneIDi114893
KEGGimmu:114893
UCSCiuc008oyw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiDCNL1_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ73
Secondary accession number(s): Q3TMX2, Q8CDZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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