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Protein

Protein phosphatase 1D

Gene

Ppm1d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-18' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi98 – 981Manganese 1By similarity
Metal bindingi98 – 981Manganese 2By similarity
Metal bindingi99 – 991Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi307 – 3071Manganese 2By similarity
Metal bindingi359 – 3591Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

  • G2/M transition of mitotic cell cycle Source: MGI
  • peptidyl-threonine dephosphorylation Source: MGI
  • response to bacterium Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1D (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform delta
Short name:
PP2C-delta
Protein phosphatase magnesium-dependent 1 delta
p53-induced protein phosphatase 1
Gene namesi
Name:Ppm1d
Synonyms:Wip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1858214. Ppm1d.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Protein phosphatase 1DPRO_0000057753Add
BLAST

Proteomic databases

MaxQBiQ9QZ67.
PaxDbiQ9QZ67.
PRIDEiQ9QZ67.

PTM databases

PhosphoSiteiQ9QZ67.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Inductioni

By p53.

Gene expression databases

BgeeiQ9QZ67.
ExpressionAtlasiQ9QZ67. baseline and differential.
GenevisibleiQ9QZ67. MM.

Interactioni

Subunit structurei

Interacts with CHEK1 and CHEK2; dephosphorylates them.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020835.

Structurei

3D structure databases

ProteinModelPortaliQ9QZ67.
SMRiQ9QZ67. Positions 92-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 368312PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9494Interaction with CHEK1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00770000120571.
HOVERGENiHBG058897.
InParanoidiQ9QZ67.
KOiK10147.
OMAiNTIMDQK.
OrthoDBiEOG7Z95M0.
TreeFamiTF313481.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QZ67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLYSLGVS VFSDQGGRKY MEDVTQIVVE PEPAAEDKPA PVPRRALGLP
60 70 80 90 100
ATPTLAGVGP SEKGPAAARD PAPDAAASLP AGRCCRRRSS VAFFAVCDGH
110 120 130 140 150
GGREAAQFAR EHLWGFIKKQ KGFTSSEPAK VCAAIRKGFL ACHLAMWKKL
160 170 180 190 200
AEWPKTMTGL PSTSGTTASV VIIRGMKMYV AHVGDSGVVL GIQDDPKDDF
210 220 230 240 250
VRAVEVTQDH KPELPKERER IEGLGGSVMN KSGVNRVVWK RPRLTHSGPV
260 270 280 290 300
RRSTVIDQIP FLAVARALGD LWSYDFFSGK FVVSPEPDTS VHTLDPRKHK
310 320 330 340 350
YIILGSDGLW NMVPPQDAIS MCQDQEEKKY LMGEQGQSCA KMLVNRALGR
360 370 380 390 400
WRQRMLRADN TSAIVICISP EVDNQGNFTN EDELFLNLTD SPTYNSQETC
410 420 430 440 450
VMTSSPSSTP PIKSPEEDAW PRLSSKDHIP ALVRSNAFSE KFLEVPAEIA
460 470 480 490 500
RGNIQTVVMT SKDSETLEEN CPKALTLRIH DSLNNTLSVG LIPTNSTNTI
510 520 530 540 550
MDQKNLKMST PGQMKAQEVE RTPPANFKRT LEESNSGPLM KKHRRNGLSR
560 570 580 590
SSGAQASSLP TASQRRHSVK LTLRRRLRGQ RKMGNPLLHQ HRKTVCVC
Length:598
Mass (Da):65,723
Last modified:July 27, 2011 - v2
Checksum:i4DB70375685BBC15
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti532 – 5321E → K in AAF09251 (PubMed:10756097).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200464 mRNA. Translation: AAF09251.1.
BX323026 Genomic DNA. Translation: CAI26197.1.
CCDSiCCDS25194.1.
RefSeqiNP_058606.3. NM_016910.3.
UniGeneiMm.45609.

Genome annotation databases

EnsembliENSMUST00000020835; ENSMUSP00000020835; ENSMUSG00000020525.
GeneIDi53892.
KEGGimmu:53892.
UCSCiuc007krl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200464 mRNA. Translation: AAF09251.1.
BX323026 Genomic DNA. Translation: CAI26197.1.
CCDSiCCDS25194.1.
RefSeqiNP_058606.3. NM_016910.3.
UniGeneiMm.45609.

3D structure databases

ProteinModelPortaliQ9QZ67.
SMRiQ9QZ67. Positions 92-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020835.

PTM databases

PhosphoSiteiQ9QZ67.

Proteomic databases

MaxQBiQ9QZ67.
PaxDbiQ9QZ67.
PRIDEiQ9QZ67.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020835; ENSMUSP00000020835; ENSMUSG00000020525.
GeneIDi53892.
KEGGimmu:53892.
UCSCiuc007krl.2. mouse.

Organism-specific databases

CTDi8493.
MGIiMGI:1858214. Ppm1d.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00770000120571.
HOVERGENiHBG058897.
InParanoidiQ9QZ67.
KOiK10147.
OMAiNTIMDQK.
OrthoDBiEOG7Z95M0.
TreeFamiTF313481.

Miscellaneous databases

NextBioi310783.
PROiQ9QZ67.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QZ67.
ExpressionAtlasiQ9QZ67. baseline and differential.
GenevisibleiQ9QZ67. MM.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The structure and expression of the murine wildtype p53-induced phosphatase 1 (Wip1) gene."
    Choi J., Appella E., Donehower L.A.
    Genomics 64:298-306(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiPPM1D_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ67
Secondary accession number(s): B1B0B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.