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Q9QZ67 (PPM1D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1D

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2C isoform delta
Short name=PP2C-delta
Protein phosphatase magnesium-dependent 1 delta
p53-induced protein phosphatase 1
Gene names
Name:Ppm1d
Synonyms:Wip1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-18' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Interacts with CHEK1 and CHEK2; dephosphorylates them By similarity.

Tissue specificity

Ubiquitously expressed. Ref.1

Induction

By p53.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 598598Protein phosphatase 1D
PRO_0000057753

Regions

Domain15 – 371357PP2C-like
Region1 – 9494Interaction with CHEK1 By similarity

Sites

Metal binding981Manganese 1 By similarity
Metal binding981Manganese 2 By similarity
Metal binding991Manganese 1; via carbonyl oxygen By similarity
Metal binding3071Manganese 2 By similarity
Metal binding3591Manganese 2 By similarity

Experimental info

Sequence conflict5321E → K in AAF09251. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9QZ67 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 4DB70375685BBC15

FASTA59865,723
        10         20         30         40         50         60 
MAGLYSLGVS VFSDQGGRKY MEDVTQIVVE PEPAAEDKPA PVPRRALGLP ATPTLAGVGP 

        70         80         90        100        110        120 
SEKGPAAARD PAPDAAASLP AGRCCRRRSS VAFFAVCDGH GGREAAQFAR EHLWGFIKKQ 

       130        140        150        160        170        180 
KGFTSSEPAK VCAAIRKGFL ACHLAMWKKL AEWPKTMTGL PSTSGTTASV VIIRGMKMYV 

       190        200        210        220        230        240 
AHVGDSGVVL GIQDDPKDDF VRAVEVTQDH KPELPKERER IEGLGGSVMN KSGVNRVVWK 

       250        260        270        280        290        300 
RPRLTHSGPV RRSTVIDQIP FLAVARALGD LWSYDFFSGK FVVSPEPDTS VHTLDPRKHK 

       310        320        330        340        350        360 
YIILGSDGLW NMVPPQDAIS MCQDQEEKKY LMGEQGQSCA KMLVNRALGR WRQRMLRADN 

       370        380        390        400        410        420 
TSAIVICISP EVDNQGNFTN EDELFLNLTD SPTYNSQETC VMTSSPSSTP PIKSPEEDAW 

       430        440        450        460        470        480 
PRLSSKDHIP ALVRSNAFSE KFLEVPAEIA RGNIQTVVMT SKDSETLEEN CPKALTLRIH 

       490        500        510        520        530        540 
DSLNNTLSVG LIPTNSTNTI MDQKNLKMST PGQMKAQEVE RTPPANFKRT LEESNSGPLM 

       550        560        570        580        590 
KKHRRNGLSR SSGAQASSLP TASQRRHSVK LTLRRRLRGQ RKMGNPLLHQ HRKTVCVC 

« Hide

References

« Hide 'large scale' references
[1]"The structure and expression of the murine wildtype p53-induced phosphatase 1 (Wip1) gene."
Choi J., Appella E., Donehower L.A.
Genomics 64:298-306(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: 129/SvJ.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF200464 mRNA. Translation: AAF09251.1.
BX323026 Genomic DNA. Translation: CAI26197.1.
CCDSCCDS25194.1.
RefSeqNP_058606.3. NM_016910.3.
UniGeneMm.45609.

3D structure databases

ProteinModelPortalQ9QZ67.
SMRQ9QZ67. Positions 92-367.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000020835.

PTM databases

PhosphoSiteQ9QZ67.

Proteomic databases

PaxDbQ9QZ67.
PRIDEQ9QZ67.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020835; ENSMUSP00000020835; ENSMUSG00000020525.
GeneID53892.
KEGGmmu:53892.
UCSCuc007krl.2. mouse.

Organism-specific databases

CTD8493.
MGIMGI:1858214. Ppm1d.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00730000110861.
HOVERGENHBG058897.
InParanoidB1B0B0.
KOK10147.
OMAVIPSKDP.
OrthoDBEOG7Z95M0.
TreeFamTF313481.

Gene expression databases

ArrayExpressQ9QZ67.
BgeeQ9QZ67.
GenevestigatorQ9QZ67.

Family and domain databases

Gene3D3.60.40.10. 2 hits.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 2 hits.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio310783.
PROQ9QZ67.
SOURCESearch...

Entry information

Entry namePPM1D_MOUSE
AccessionPrimary (citable) accession number: Q9QZ67
Secondary accession number(s): B1B0B0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot