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Protein

Protein DBF4 homolog A

Gene

Dbf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri288 – 33649DBF4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68962. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DBF4 homolog A
Alternative name(s):
MuDBF4
Gene namesi
Name:Dbf4
Synonyms:Dbf4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1351328. Dbf4.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663Protein DBF4 homolog APRO_0000234062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei311 – 3111PhosphoserineBy similarity
Modified residuei344 – 3441PhosphothreonineBy similarity
Modified residuei353 – 3531PhosphoserineBy similarity
Modified residuei358 – 3581PhosphoserineBy similarity
Modified residuei412 – 4121PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QZ41.
PRIDEiQ9QZ41.

PTM databases

iPTMnetiQ9QZ41.
PhosphoSiteiQ9QZ41.

Expressioni

Inductioni

In a cell cycle-dependent manner. Induced at low level through G1. Increased during S phase and decreased at the end of S phase.1 Publication

Gene expression databases

BgeeiENSMUSG00000002297.
CleanExiMM_DBF4.
ExpressionAtlasiQ9QZ41. baseline and differential.
GenevisibleiQ9QZ41. MM.

Interactioni

Subunit structurei

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132906.

Structurei

3D structure databases

ProteinModelPortaliQ9QZ41.
SMRiQ9QZ41. Positions 214-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 12889BRCT 1Add
BLAST
Domaini154 – 17926BRCT 2Add
BLAST

Sequence similaritiesi

Contains 2 BRCT domains.Curated
Contains 1 DBF4-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri288 – 33649DBF4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4139. Eukaryota.
COG5067. LUCA.
GeneTreeiENSGT00530000063909.
HOGENOMiHOG000060164.
HOVERGENiHBG063274.
InParanoidiQ9QZ41.
KOiK06629.
OMAiDIWEEEN.
OrthoDBiEOG091G06P9.
PhylomeDBiQ9QZ41.
TreeFamiTF332790.

Family and domain databases

Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QZ41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLETMRIHS KAPLPGGIQD RNEKNRPSLK SLKADNRLEK SKYKPLWGKI
60 70 80 90 100
FYLDLPSITI CEKLQKDIKE LGGRVEEFLS KDISYFVSNK KEAKYAQTLG
110 120 130 140 150
RVSPVPSPES AYTAETTSPH PSHDGSSFKS QDRVCLSRGK LLAEKAVKDH
160 170 180 190 200
DFIPANSILS NALSWGVKIL HIDDIRYYIE QKKKALSALK KSSASGKDAG
210 220 230 240 250
KKAGPGIQKT RTGRLKKPFL KVEDVNRCYR PFYLQLPSLP CINYFLQKPC
260 270 280 290 300
SPFDIEKSSS VQKQAQPKLR INMDGDKCGT PVQLQLKEKR KKGYCECCLQ
310 320 330 340 350
KYEDLETHLL SEKHRNFAQS NQYQVVDDIV SQLVFDFVEY GRDTPQKKRI
360 370 380 390 400
RYSVGSLSSV SANVLKNTAP KEKPLLEPNF QKDVGESSGH LLKPNSQYEE
410 420 430 440 450
TQKPEEKHGF ASEPTTYSSA GLKGCDRKPV SMFNASEPDP EQEYAQLPLR
460 470 480 490 500
DSTPEHQVTE GRNDGEQRVD PAPGVSQSCG QVSHLSTESN LPQPQLAADI
510 520 530 540 550
TQLSAKDLQE KGFHVVIGHA SDLVALNTSK EQLTMKARTP PCSPQEPHEC
560 570 580 590 600
DTENMENLPC GKIQRKVRML LGQQKANAEP SAELDKKRTE YLPAHEDRTC
610 620 630 640 650
GSPVQSLLDL FQTSEEKSEF LGFTGYTENS GICDVLDIWE EENSSTLLST
660
FFSSPSTSAF VGF
Length:663
Mass (Da):74,176
Last modified:May 1, 2000 - v1
Checksum:i72E05CB87C3B1650
GO
Isoform 2 (identifier: Q9QZ41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-213: TG → R

Note: No experimental confirmation available.
Show »
Length:662
Mass (Da):74,174
Checksum:iAB29F064CCF77202
GO
Isoform 3 (identifier: Q9QZ41-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-321: HLLSEKHRNFAQSN → VNVIFHLGLVFLKI
     322-663: Missing.

Note: No experimental confirmation available.
Show »
Length:321
Mass (Da):36,324
Checksum:i741B172CF52A4579
GO

Sequence cautioni

The sequence AAH69983 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei212 – 2132TG → R in isoform 2. 2 PublicationsVSP_018205
Alternative sequencei308 – 32114HLLSE…FAQSN → VNVIFHLGLVFLKI in isoform 3. 1 PublicationVSP_018206Add
BLAST
Alternative sequencei322 – 663342Missing in isoform 3. 1 PublicationVSP_018207Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003132 mRNA. Translation: CAB56847.1.
AK014480 mRNA. Translation: BAB29383.1.
AK132403 mRNA. Translation: BAE21148.1.
AK163408 mRNA. Translation: BAE37338.1.
BC069983 mRNA. Translation: AAH69983.1. Sequence problems.
BC145392 mRNA. Translation: AAI45393.1.
CCDSiCCDS19081.1. [Q9QZ41-1]
CCDS57324.1. [Q9QZ41-2]
RefSeqiNP_001177646.1. NM_001190717.1. [Q9QZ41-2]
NP_038754.1. NM_013726.3. [Q9QZ41-1]
UniGeneiMm.292470.

Genome annotation databases

EnsembliENSMUST00000002368; ENSMUSP00000002368; ENSMUSG00000002297. [Q9QZ41-2]
ENSMUST00000171808; ENSMUSP00000132906; ENSMUSG00000002297. [Q9QZ41-1]
GeneIDi27214.
KEGGimmu:27214.
UCSCiuc008wkc.2. mouse. [Q9QZ41-1]
uc008wkd.2. mouse. [Q9QZ41-2]
uc008wke.2. mouse. [Q9QZ41-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003132 mRNA. Translation: CAB56847.1.
AK014480 mRNA. Translation: BAB29383.1.
AK132403 mRNA. Translation: BAE21148.1.
AK163408 mRNA. Translation: BAE37338.1.
BC069983 mRNA. Translation: AAH69983.1. Sequence problems.
BC145392 mRNA. Translation: AAI45393.1.
CCDSiCCDS19081.1. [Q9QZ41-1]
CCDS57324.1. [Q9QZ41-2]
RefSeqiNP_001177646.1. NM_001190717.1. [Q9QZ41-2]
NP_038754.1. NM_013726.3. [Q9QZ41-1]
UniGeneiMm.292470.

3D structure databases

ProteinModelPortaliQ9QZ41.
SMRiQ9QZ41. Positions 214-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132906.

PTM databases

iPTMnetiQ9QZ41.
PhosphoSiteiQ9QZ41.

Proteomic databases

PaxDbiQ9QZ41.
PRIDEiQ9QZ41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002368; ENSMUSP00000002368; ENSMUSG00000002297. [Q9QZ41-2]
ENSMUST00000171808; ENSMUSP00000132906; ENSMUSG00000002297. [Q9QZ41-1]
GeneIDi27214.
KEGGimmu:27214.
UCSCiuc008wkc.2. mouse. [Q9QZ41-1]
uc008wkd.2. mouse. [Q9QZ41-2]
uc008wke.2. mouse. [Q9QZ41-3]

Organism-specific databases

CTDi10926.
MGIiMGI:1351328. Dbf4.

Phylogenomic databases

eggNOGiKOG4139. Eukaryota.
COG5067. LUCA.
GeneTreeiENSGT00530000063909.
HOGENOMiHOG000060164.
HOVERGENiHBG063274.
InParanoidiQ9QZ41.
KOiK06629.
OMAiDIWEEEN.
OrthoDBiEOG091G06P9.
PhylomeDBiQ9QZ41.
TreeFamiTF332790.

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68962. Activation of the pre-replicative complex.

Miscellaneous databases

ChiTaRSiDbf4. mouse.
PROiQ9QZ41.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002297.
CleanExiMM_DBF4.
ExpressionAtlasiQ9QZ41. baseline and differential.
GenevisibleiQ9QZ41. MM.

Family and domain databases

Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBF4A_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ41
Secondary accession number(s): B7ZNS0
, Q3TQP4, Q3V1K2, Q6NSQ1, Q9CXF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.