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Protein

Protein DBF4 homolog A

Gene

Dbf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri288 – 336DBF4-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, DNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-176187 Activation of ATR in response to replication stress
R-MMU-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DBF4 homolog A
Alternative name(s):
MuDBF4
Gene namesi
Name:Dbf4
Synonyms:Dbf4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1351328 Dbf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002340621 – 663Protein DBF4 homolog AAdd BLAST663

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei311PhosphoserineBy similarity1
Modified residuei344PhosphothreonineBy similarity1
Modified residuei353PhosphoserineBy similarity1
Modified residuei358PhosphoserineBy similarity1
Modified residuei412PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QZ41
PRIDEiQ9QZ41

PTM databases

iPTMnetiQ9QZ41
PhosphoSitePlusiQ9QZ41

Expressioni

Inductioni

In a cell cycle-dependent manner. Induced at low level through G1. Increased during S phase and decreased at the end of S phase.1 Publication

Gene expression databases

BgeeiENSMUSG00000002297
CleanExiMM_DBF4
ExpressionAtlasiQ9QZ41 baseline and differential
GenevisibleiQ9QZ41 MM

Interactioni

Subunit structurei

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132906

Structurei

3D structure databases

ProteinModelPortaliQ9QZ41
SMRiQ9QZ41
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 128BRCT 1Add BLAST89
Domaini154 – 179BRCT 2Add BLAST26

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri288 – 336DBF4-typePROSITE-ProRule annotationAdd BLAST49

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4139 Eukaryota
COG5067 LUCA
GeneTreeiENSGT00530000063909
HOGENOMiHOG000060164
HOVERGENiHBG063274
InParanoidiQ9QZ41
KOiK06629
OMAiDIWEEEN
OrthoDBiEOG091G06P9
PhylomeDBiQ9QZ41
TreeFamiTF332790

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.30.160.680, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR006572 Znf_DBF
IPR038545 Znf_DBF_sf
PfamiView protein in Pfam
PF07535 zf-DBF, 1 hit
SMARTiView protein in SMART
SM00586 ZnF_DBF, 1 hit
PROSITEiView protein in PROSITE
PS51265 ZF_DBF4, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QZ41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLETMRIHS KAPLPGGIQD RNEKNRPSLK SLKADNRLEK SKYKPLWGKI
60 70 80 90 100
FYLDLPSITI CEKLQKDIKE LGGRVEEFLS KDISYFVSNK KEAKYAQTLG
110 120 130 140 150
RVSPVPSPES AYTAETTSPH PSHDGSSFKS QDRVCLSRGK LLAEKAVKDH
160 170 180 190 200
DFIPANSILS NALSWGVKIL HIDDIRYYIE QKKKALSALK KSSASGKDAG
210 220 230 240 250
KKAGPGIQKT RTGRLKKPFL KVEDVNRCYR PFYLQLPSLP CINYFLQKPC
260 270 280 290 300
SPFDIEKSSS VQKQAQPKLR INMDGDKCGT PVQLQLKEKR KKGYCECCLQ
310 320 330 340 350
KYEDLETHLL SEKHRNFAQS NQYQVVDDIV SQLVFDFVEY GRDTPQKKRI
360 370 380 390 400
RYSVGSLSSV SANVLKNTAP KEKPLLEPNF QKDVGESSGH LLKPNSQYEE
410 420 430 440 450
TQKPEEKHGF ASEPTTYSSA GLKGCDRKPV SMFNASEPDP EQEYAQLPLR
460 470 480 490 500
DSTPEHQVTE GRNDGEQRVD PAPGVSQSCG QVSHLSTESN LPQPQLAADI
510 520 530 540 550
TQLSAKDLQE KGFHVVIGHA SDLVALNTSK EQLTMKARTP PCSPQEPHEC
560 570 580 590 600
DTENMENLPC GKIQRKVRML LGQQKANAEP SAELDKKRTE YLPAHEDRTC
610 620 630 640 650
GSPVQSLLDL FQTSEEKSEF LGFTGYTENS GICDVLDIWE EENSSTLLST
660
FFSSPSTSAF VGF
Length:663
Mass (Da):74,176
Last modified:May 1, 2000 - v1
Checksum:i72E05CB87C3B1650
GO
Isoform 2 (identifier: Q9QZ41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-213: TG → R

Note: No experimental confirmation available.
Show »
Length:662
Mass (Da):74,174
Checksum:iAB29F064CCF77202
GO
Isoform 3 (identifier: Q9QZ41-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-321: HLLSEKHRNFAQSN → VNVIFHLGLVFLKI
     322-663: Missing.

Note: No experimental confirmation available.
Show »
Length:321
Mass (Da):36,324
Checksum:i741B172CF52A4579
GO

Sequence cautioni

The sequence AAH69983 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018205212 – 213TG → R in isoform 2. 2 Publications2
Alternative sequenceiVSP_018206308 – 321HLLSE…FAQSN → VNVIFHLGLVFLKI in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_018207322 – 663Missing in isoform 3. 1 PublicationAdd BLAST342

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003132 mRNA Translation: CAB56847.1
AK014480 mRNA Translation: BAB29383.1
AK132403 mRNA Translation: BAE21148.1
AK163408 mRNA Translation: BAE37338.1
BC069983 mRNA Translation: AAH69983.1 Sequence problems.
BC145392 mRNA Translation: AAI45393.1
CCDSiCCDS19081.1 [Q9QZ41-1]
CCDS57324.1 [Q9QZ41-2]
RefSeqiNP_001177646.1, NM_001190717.1 [Q9QZ41-2]
NP_038754.1, NM_013726.3 [Q9QZ41-1]
UniGeneiMm.292470

Genome annotation databases

EnsembliENSMUST00000002368; ENSMUSP00000002368; ENSMUSG00000002297 [Q9QZ41-2]
ENSMUST00000171808; ENSMUSP00000132906; ENSMUSG00000002297 [Q9QZ41-1]
GeneIDi27214
KEGGimmu:27214
UCSCiuc008wkc.2 mouse [Q9QZ41-1]
uc008wkd.2 mouse [Q9QZ41-2]
uc008wke.2 mouse [Q9QZ41-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDBF4A_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ41
Secondary accession number(s): B7ZNS0
, Q3TQP4, Q3V1K2, Q6NSQ1, Q9CXF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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