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Protein

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1

Gene

St6galnac1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transfers CMP-NeuAc with an alpha-2,6-linkage to the GalNAc residues of GalNAc-O-Ser/Thr, Gal-beta-1,3-GalNAc-O-Ser/Thr and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-O-Ser/Thr are substrates. Higher activity towards GalNAc-O-Ser/Thr.1 Publication

Catalytic activityi

CMP-N-acetylneuraminate + glycano-(1->3)-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein = CMP + glycano-((2->6)-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity Source: MGI

GO - Biological processi

  • ganglioside biosynthetic process Source: MGI
  • oligosaccharide biosynthetic process Source: MGI
  • protein N-linked glycosylation via asparagine Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 (EC:2.4.99.3)
Alternative name(s):
GalNAc alpha-2,6-sialyltransferase I
ST6GalNAc I
Short name:
ST6GalNAcI
Sialyltransferase 7A
Short name:
SIAT7-A
Gene namesi
Name:St6galnac1
Synonyms:Siat7a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1341826. St6galnac1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini34 – 526493LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1PRO_0000149270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence analysis
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence analysis
Disulfide bondi293 ↔ 461By similarity
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis
Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9QZ39.
PaxDbiQ9QZ39.
PRIDEiQ9QZ39.

PTM databases

iPTMnetiQ9QZ39.
PhosphoSiteiQ9QZ39.

Expressioni

Tissue specificityi

Submaxillary gland, mammary gland, spleen and colon.

Gene expression databases

BgeeiQ9QZ39.
GenevisibleiQ9QZ39. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000009732.

Structurei

3D structure databases

ProteinModelPortaliQ9QZ39.
SMRiQ9QZ39. Positions 239-515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000231097.
HOVERGENiHBG073351.
InParanoidiQ9QZ39.
KOiK03479.
OMAiWLQNIFL.
OrthoDBiEOG7J17ZK.
TreeFamiTF354325.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QZ39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRYCRGLSQ RQAFLLLTVL ALLFILLFVV KDPRAKDSRC QFIWKNDASA
60 70 80 90 100
QENQQKAEPQ VPIMTLSPRV HNKETTSVSS KDLKKQEREA VQGEQAEGKE
110 120 130 140 150
KRKLETIRPA PENPQSKAEP AAKTPVSEHL DKLPRAPGAL STRKTPMATG
160 170 180 190 200
AVPAKKKVVQ ATKSPASSPH PTTRRRQRLK ASEFKSEPRW DFEEEYSLDM
210 220 230 240 250
SSLQTNCSAS VKIKASKSPW LQNIFLPNIT LFLDSGRFTQ SEWNRLEHFA
260 270 280 290 300
PPFGFMELNQ SLVQKVVTRF PPVRQQQLLL ASLPTGYSKC ITCAVVGNGG
310 320 330 340 350
ILNDSRVGRE IDSHDYVFRL SGAVIKGYEQ DVGTRTSFYG FTAFSLTQSI
360 370 380 390 400
LILGRRGFQH VPLGKDVRYL HFLEGTRDYE WLEAMFLNQT LAKTHLSWFR
410 420 430 440 450
HRPQEAFRNA LDLDRYLLLH PDFLRYMKNR FLRSKTLDTA HWRIYRPTTG
460 470 480 490 500
ALLLLTALHL CDKVSAYGFI TEGHQRFSDH YYDTSWKRLI FYINHDFRLE
510 520
RMVWKRLHDE GIIWLYQRPQ SDKAKN
Length:526
Mass (Da):60,734
Last modified:July 27, 2011 - v2
Checksum:i682A5E35E3C142D6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401C → R in CAA72137 (PubMed:10788794).Curated
Sequence conflicti44 – 452WK → LN in CAA72137 (PubMed:10788794).Curated
Sequence conflicti48 – 481A → S in CAA72137 (PubMed:10788794).Curated
Sequence conflicti53 – 542NQ → IL in CAA72137 (PubMed:10788794).Curated
Sequence conflicti61 – 611V → G in CAA72137 (PubMed:10788794).Curated
Sequence conflicti75 – 751T → A in CAA72137 (PubMed:10788794).Curated
Sequence conflicti133 – 1331L → V in CAA72137 (PubMed:10788794).Curated
Sequence conflicti136 – 1361A → T in CAA72137 (PubMed:10788794).Curated
Sequence conflicti378 – 3781D → N in CAA72137 (PubMed:10788794).Curated
Sequence conflicti475 – 4751Q → E in CAA72137 (PubMed:10788794).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11274 mRNA. Translation: CAA72137.1.
Y10294 Genomic DNA. Translation: CAA71341.1.
AL645542 Genomic DNA. Translation: CAM25277.1.
BC141095 mRNA. Translation: AAI41096.1.
BC145373 mRNA. Translation: AAI45374.1.
CCDSiCCDS36380.1.
PIRiJC7248.
RefSeqiNP_035501.2. NM_011371.2.
UniGeneiMm.436887.
Mm.491644.

Genome annotation databases

EnsembliENSMUST00000009732; ENSMUSP00000009732; ENSMUSG00000009588.
GeneIDi20445.
KEGGimmu:20445.
UCSCiuc007mme.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST6GalNAc I

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11274 mRNA. Translation: CAA72137.1.
Y10294 Genomic DNA. Translation: CAA71341.1.
AL645542 Genomic DNA. Translation: CAM25277.1.
BC141095 mRNA. Translation: AAI41096.1.
BC145373 mRNA. Translation: AAI45374.1.
CCDSiCCDS36380.1.
PIRiJC7248.
RefSeqiNP_035501.2. NM_011371.2.
UniGeneiMm.436887.
Mm.491644.

3D structure databases

ProteinModelPortaliQ9QZ39.
SMRiQ9QZ39. Positions 239-515.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000009732.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiQ9QZ39.
PhosphoSiteiQ9QZ39.

Proteomic databases

MaxQBiQ9QZ39.
PaxDbiQ9QZ39.
PRIDEiQ9QZ39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009732; ENSMUSP00000009732; ENSMUSG00000009588.
GeneIDi20445.
KEGGimmu:20445.
UCSCiuc007mme.1. mouse.

Organism-specific databases

CTDi55808.
MGIiMGI:1341826. St6galnac1.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000231097.
HOVERGENiHBG073351.
InParanoidiQ9QZ39.
KOiK03479.
OMAiWLQNIFL.
OrthoDBiEOG7J17ZK.
TreeFamiTF354325.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-4085001. Sialic acid metabolism.

Miscellaneous databases

ChiTaRSiSt6galnac1. mouse.
PROiQ9QZ39.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QZ39.
GenevisibleiQ9QZ39. MM.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and genomic analysis of mouse GalNAc alpha-2,6-sialyltransferase (ST6GalNAc I)."
    Kurosawa N., Takashima S., Kono M., Ikehara Y., Inoue M., Tachida Y., Narimatsu H., Tsuji S.
    J. Biochem. 127:845-854(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION.
    Tissue: Submandibular gland.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiSIA7A_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ39
Secondary accession number(s): A2AA23, Q9JJP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.