Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial

Gene

Nfu1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Iron-sulfur cluster scaffold protein which can assemble [4Fe-2S] clusters and deliver them to target proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi210Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1
Metal bindingi213Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandIron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
Alternative name(s):
HIRA-interacting protein 5
Short name:
mHIRIP5
Gene namesi
Name:Nfu1
Synonyms:Hirip5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1913290. Nfu1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
ChainiPRO_000016619230 – 255NFU1 iron-sulfur cluster scaffold homolog, mitochondrialAdd BLAST226

Proteomic databases

EPDiQ9QZ23.
MaxQBiQ9QZ23.
PaxDbiQ9QZ23.
PeptideAtlasiQ9QZ23.
PRIDEiQ9QZ23.

PTM databases

PhosphoSitePlusiQ9QZ23.
SwissPalmiQ9QZ23.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029993.
ExpressionAtlasiQ9QZ23. baseline and differential.

Interactioni

Subunit structurei

Monomer and homohexamer; the apo-NFU1 is a monomer, while the holo-NFU1 is a hexamer composed of a trimer of dimer that is probably linked by some 4Fe-4S cluster. Interacts with HIRA and EPM2A/laforin. Interacts with BOLA3.By similarity

Protein-protein interaction databases

IntActiQ9QZ23. 1 interactor.
MINTiMINT-1853650.
STRINGi10090.ENSMUSP00000032060.

Structurei

Secondary structure

1255
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi168 – 179Combined sources12
Helixi182 – 188Combined sources7
Beta strandi194 – 198Combined sources5
Beta strandi201 – 204Combined sources4
Helixi214 – 219Combined sources6
Helixi221 – 231Combined sources11
Beta strandi238 – 240Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEHNMR-A163-241[»]
ProteinModelPortaliQ9QZ23.
SMRiQ9QZ23.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QZ23.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 241NifUAdd BLAST69

Sequence similaritiesi

Belongs to the NifU family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2358. Eukaryota.
COG0694. LUCA.
GeneTreeiENSGT00390000011296.
HOGENOMiHOG000023557.
HOVERGENiHBG054438.
InParanoidiQ9QZ23.
PhylomeDBiQ9QZ23.
TreeFamiTF315076.

Family and domain databases

Gene3Di3.30.1370.70. 1 hit.
3.30.300.130. 1 hit.
InterProiView protein in InterPro
IPR034904. FSCA_dom.
IPR014824. Nfu/NifU_N.
IPR017065. NFU1.
IPR035433. NFU1-like.
IPR001075. NIF_FeS_clus_asmbl_NifU_C.
PANTHERiPTHR11178:SF30. PTHR11178:SF30. 1 hit.
PfamiView protein in Pfam
PF08712. Nfu_N. 1 hit.
PF01106. NifU. 1 hit.
PIRSFiPIRSF036773. HIRIP5. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002830. NIF_FeS_clus_asmbl_NifU_C. 1 hit.
SMARTiView protein in SMART
SM00932. Nfu_N. 1 hit.
SUPFAMiSSF110836. SSF110836. 1 hit.
SSF117916. SSF117916. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZ23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAERAWGA AVGVVRLCRR FCHVATPHTF KKQPLHQYVR RPLFPLRAPL
60 70 80 90 100
CNTVRFMFIQ TQDTPNPNSL KFIPGKPVLE TRTMDFPTPA AAFRSPLARQ
110 120 130 140 150
LFRIEGVKSV FFGPDFITVT KENEELDWNL LKPDIYATIM DFFASGLPLV
160 170 180 190 200
TEETPPPPGE AGSEEDDEVV AMIKELLDTR IRPTVQEDGG DVIYRGFEDG
210 220 230 240 250
IVRLKLQGSC TSCPSSIITL KSGIQNMLQF YIPEVEGVEQ VMDDDESDEK

EANSS
Length:255
Mass (Da):28,568
Last modified:July 22, 2008 - v2
Checksum:iE7DA6A0333E2EB5E
GO

Sequence cautioni

The sequence AAH18355 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC24985 differs from that shown. Reason: Frameshift at positions 33 and 48.Curated
The sequence CAB57314 differs from that shown. Reason: Frameshift at positions 5, 8 and 50.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11 – 12AV → TS in AAQ73785 (PubMed:12915448).Curated2
Sequence conflicti19R → K in CAB57314 (PubMed:11342215).Curated1
Sequence conflicti39V → A in CAB57314 (PubMed:11342215).Curated1
Sequence conflicti39V → A in AAH18355 (PubMed:15489334).Curated1
Sequence conflicti39V → A in AAQ73785 (PubMed:12915448).Curated1
Sequence conflicti48A → H in CAB57314 (PubMed:11342215).Curated1
Sequence conflicti163S → SS in BAC24985 (PubMed:16141072).Curated1
Sequence conflicti163S → SS in BAE26508 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002244 mRNA. Translation: BAC24985.1. Frameshift.
AK003730 mRNA. Translation: BAB22965.1.
AK145558 mRNA. Translation: BAE26508.1.
AJ132616 mRNA. Translation: CAB57314.1. Frameshift.
BC018355 mRNA. Translation: AAH18355.1. Different initiation.
AY335195 mRNA. Translation: AAQ73785.1.
CCDSiCCDS85088.1.
RefSeqiNP_001164062.1. NM_001170591.1.
NP_064429.2. NM_020045.3.
XP_006506482.1. XM_006506419.1.
XP_006506483.1. XM_006506420.1.
UniGeneiMm.23809.

Genome annotation databases

EnsembliENSMUST00000117583; ENSMUSP00000113332; ENSMUSG00000029993.
GeneIDi56748.
KEGGimmu:56748.
UCSCiuc009csv.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiNFU1_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ23
Secondary accession number(s): Q3ULE0
, Q6VNZ7, Q8CF80, Q9D1B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: July 22, 2008
Last modified: August 30, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families