Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9QZ08 (NAGK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetyl-D-glucosamine kinase

Short name=N-acetylglucosamine kinase
EC=2.7.1.59
Alternative name(s):
GlcNAc kinase
Gene names
Name:Nagk
Synonyms:Gnk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity. Ref.1

Catalytic activity

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.

Pathway

Amino-sugar metabolism; N-acetylneuraminate degradation. Ref.3

Subunit structure

Homodimer By similarity.

Tissue specificity

Ubiquitous. Ref.1

Sequence similarities

Belongs to the eukaryotic-type N-acetylglucosamine kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 343342N-acetyl-D-glucosamine kinase
PRO_0000096697

Regions

Nucleotide binding6 – 138ATP By similarity
Region129 – 1302Substrate binding By similarity
Region145 – 1473Substrate binding By similarity

Sites

Binding site361Substrate By similarity
Binding site1071Substrate By similarity
Binding site1521Substrate By similarity
Binding site2711ATP By similarity
Binding site2751ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue761Phosphoserine By similarity
Modified residue2051Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QZ08 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A3832E98C9B3228E

FASTA34337,268
        10         20         30         40         50         60 
MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTDQCVERIN EMVDRAKQKA 

        70         80         90        100        110        120 
GVDPLVPLRS LGLSLSGGEQ EDAVRLLIEE LRHRFPNLSE NYLITTDAAG SIATATPDGG 

       130        140        150        160        170        180 
IVLISGTGSN CRLINPDGSE SGCGGWGHMM GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD 

       190        200        210        220        230        240 
IGHVKQAMFD YFQVPDRLGI LTHLYRDFDK CKFAGFCQKI AEGAHQGDPL SRYIFRKAGE 

       250        260        270        280        290        300 
MLGRHVVAVL PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTLG REQQAQNSFS 

       310        320        330        340 
SFTLMKLRHS SALGGASLGA RHIGYHLPMD YSINAIAFYS YTF 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of murine and human N-acetylglucosamine kinase."
Hinderlich S., Berger M., Schwarzkopf M., Effertz K., Reutter W.
Eur. J. Biochem. 267:3301-3308(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid."
Bergfeld A.K., Pearce O.M., Diaz S.L., Pham T., Varki A.
J. Biol. Chem. 287:28865-28881(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: PATHWAY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ242909 mRNA. Translation: CAB61849.1.
BC004689 mRNA. Translation: AAH04689.1.
CCDSCCDS20286.1.
RefSeqNP_062415.1. NM_019542.2.
UniGeneMm.190650.

3D structure databases

ProteinModelPortalQ9QZ08.
SMRQ9QZ08. Positions 2-343.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9QZ08. 1 interaction.
MINTMINT-224654.

PTM databases

PhosphoSiteQ9QZ08.

2D gel databases

REPRODUCTION-2DPAGEQ9QZ08.

Proteomic databases

MaxQBQ9QZ08.
PaxDbQ9QZ08.
PRIDEQ9QZ08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037376; ENSMUSP00000042026; ENSMUSG00000034744.
GeneID56174.
KEGGmmu:56174.
UCSCuc009coh.2. mouse.

Organism-specific databases

CTD55577.
MGIMGI:1860418. Nagk.

Phylogenomic databases

eggNOGCOG2971.
GeneTreeENSGT00510000047418.
HOGENOMHOG000007248.
HOVERGENHBG052570.
KOK00884.
OrthoDBEOG7V1FR7.
PhylomeDBQ9QZ08.
TreeFamTF314158.

Enzyme and pathway databases

BRENDA2.7.1.59. 3474.
SABIO-RKQ9QZ08.
UniPathwayUPA00629.

Gene expression databases

ArrayExpressQ9QZ08.
BgeeQ9QZ08.
CleanExMM_NAGK.
GenevestigatorQ9QZ08.

Family and domain databases

InterProIPR002731. ATPase_BadF.
[Graphical view]
PfamPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311940.
PROQ9QZ08.
SOURCESearch...

Entry information

Entry nameNAGK_MOUSE
AccessionPrimary (citable) accession number: Q9QZ08
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot