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Protein

N-acetyl-D-glucosamine kinase

Gene

Nagk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.1 Publication

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.

Pathwayi: N-acetylneuraminate degradation

This protein is involved in the pathway N-acetylneuraminate degradation, which is part of Amino-sugar metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenBy similarity1
Binding sitei36SubstrateBy similarity1
Binding sitei107SubstrateBy similarity1
Binding sitei127ATPBy similarity1
Binding sitei152SubstrateBy similarity1
Binding sitei214ATP; via carbonyl oxygenBy similarity1
Binding sitei271ATPBy similarity1
Binding sitei275ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • N-acetylglucosamine kinase activity Source: MGI
  • N-acylmannosamine kinase activity Source: MGI

GO - Biological processi

  • N-acetylglucosamine metabolic process Source: MGI
  • N-acetylneuraminate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.59. 3474.
ReactomeiR-MMU-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ9QZ08.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-D-glucosamine kinase (EC:2.7.1.59)
Short name:
N-acetylglucosamine kinase
Alternative name(s):
GlcNAc kinase
Gene namesi
Name:Nagk
Synonyms:Gnk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1860418. Nagk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000966972 – 343N-acetyl-D-glucosamine kinaseAdd BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei76PhosphoserineCombined sources1
Modified residuei205PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9QZ08.
MaxQBiQ9QZ08.
PaxDbiQ9QZ08.
PRIDEiQ9QZ08.

2D gel databases

REPRODUCTION-2DPAGEQ9QZ08.

PTM databases

iPTMnetiQ9QZ08.
PhosphoSitePlusiQ9QZ08.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000034744.
CleanExiMM_NAGK.
ExpressionAtlasiQ9QZ08. baseline and differential.
GenevisibleiQ9QZ08. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ9QZ08. 1 interactor.
MINTiMINT-224654.
STRINGi10090.ENSMUSP00000042026.

Structurei

3D structure databases

ProteinModelPortaliQ9QZ08.
SMRiQ9QZ08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 130Substrate bindingBy similarity2
Regioni145 – 147Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1794. Eukaryota.
COG2971. LUCA.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiQ9QZ08.
KOiK00884.
PhylomeDBiQ9QZ08.
TreeFamiTF314158.

Family and domain databases

InterProiIPR002731. ATPase_BadF.
[Graphical view]
PfamiPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QZ08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTDQCVERIN
60 70 80 90 100
EMVDRAKQKA GVDPLVPLRS LGLSLSGGEQ EDAVRLLIEE LRHRFPNLSE
110 120 130 140 150
NYLITTDAAG SIATATPDGG IVLISGTGSN CRLINPDGSE SGCGGWGHMM
160 170 180 190 200
GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD IGHVKQAMFD YFQVPDRLGI
210 220 230 240 250
LTHLYRDFDK CKFAGFCQKI AEGAHQGDPL SRYIFRKAGE MLGRHVVAVL
260 270 280 290 300
PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTLG REQQAQNSFS
310 320 330 340
SFTLMKLRHS SALGGASLGA RHIGYHLPMD YSINAIAFYS YTF
Length:343
Mass (Da):37,268
Last modified:January 23, 2007 - v3
Checksum:iA3832E98C9B3228E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242909 mRNA. Translation: CAB61849.1.
BC004689 mRNA. Translation: AAH04689.1.
CCDSiCCDS20286.1.
RefSeqiNP_062415.1. NM_019542.2.
UniGeneiMm.190650.

Genome annotation databases

EnsembliENSMUST00000037376; ENSMUSP00000042026; ENSMUSG00000034744.
GeneIDi56174.
KEGGimmu:56174.
UCSCiuc009coh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242909 mRNA. Translation: CAB61849.1.
BC004689 mRNA. Translation: AAH04689.1.
CCDSiCCDS20286.1.
RefSeqiNP_062415.1. NM_019542.2.
UniGeneiMm.190650.

3D structure databases

ProteinModelPortaliQ9QZ08.
SMRiQ9QZ08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QZ08. 1 interactor.
MINTiMINT-224654.
STRINGi10090.ENSMUSP00000042026.

PTM databases

iPTMnetiQ9QZ08.
PhosphoSitePlusiQ9QZ08.

2D gel databases

REPRODUCTION-2DPAGEQ9QZ08.

Proteomic databases

EPDiQ9QZ08.
MaxQBiQ9QZ08.
PaxDbiQ9QZ08.
PRIDEiQ9QZ08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037376; ENSMUSP00000042026; ENSMUSG00000034744.
GeneIDi56174.
KEGGimmu:56174.
UCSCiuc009coh.2. mouse.

Organism-specific databases

CTDi55577.
MGIiMGI:1860418. Nagk.

Phylogenomic databases

eggNOGiKOG1794. Eukaryota.
COG2971. LUCA.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiQ9QZ08.
KOiK00884.
PhylomeDBiQ9QZ08.
TreeFamiTF314158.

Enzyme and pathway databases

UniPathwayiUPA00629.
BRENDAi2.7.1.59. 3474.
ReactomeiR-MMU-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ9QZ08.

Miscellaneous databases

PROiQ9QZ08.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034744.
CleanExiMM_NAGK.
ExpressionAtlasiQ9QZ08. baseline and differential.
GenevisibleiQ9QZ08. MM.

Family and domain databases

InterProiIPR002731. ATPase_BadF.
[Graphical view]
PfamiPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAGK_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.