Q9QZ08 (NAGK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
Short name=N-acetylglucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:	Nagk
Synonyms:	Gnk

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity. Ref.1
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation. Ref.3
Subunit structure	Homodimer By similarity.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the eukaryotic-type N-acetylglucosamine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from direct assay Ref.1. Source: MGI N-acetylneuraminate catabolic process Traceable author statement Ref.3. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acetylglucosamine kinase activity Inferred from direct assay Ref.1. Source: MGI N-acylmannosamine kinase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 343	342	N-acetyl-D-glucosamine kinase		PRO_0000096697
Regions
	Nucleotide binding	6 – 13	8	ATP By similarity
	Region	129 – 130	2	Substrate binding By similarity
	Region	145 – 147	3	Substrate binding By similarity
Sites
	Binding site	36	1	Substrate By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	271	1	ATP By similarity
	Binding site	275	1	ATP By similarity
Amino acid modifications
	Modified residue	76	1	Phosphoserine By similarity
	Modified residue	205	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QZ08 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A3832E98C9B3228E	FASTA	343	37,268
	10 20 30 40 50 60 MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTDQCVERIN EMVDRAKQKA 70 80 90 100 110 120 GVDPLVPLRS LGLSLSGGEQ EDAVRLLIEE LRHRFPNLSE NYLITTDAAG SIATATPDGG 130 140 150 160 170 180 IVLISGTGSN CRLINPDGSE SGCGGWGHMM GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD 190 200 210 220 230 240 IGHVKQAMFD YFQVPDRLGI LTHLYRDFDK CKFAGFCQKI AEGAHQGDPL SRYIFRKAGE 250 260 270 280 290 300 MLGRHVVAVL PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTLG REQQAQNSFS 310 320 330 340 SFTLMKLRHS SALGGASLGA RHIGYHLPMD YSINAIAFYS YTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of murine and human N-acetylglucosamine kinase." Hinderlich S., Berger M., Schwarzkopf M., Effertz K., Reutter W. Eur. J. Biochem. 267:3301-3308(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid." Bergfeld A.K., Pearce O.M., Diaz S.L., Pham T., Varki A. J. Biol. Chem. 287:28865-28881(2012) [PubMed] [Europe PMC] [Abstract] Cited for: PATHWAY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ242909 mRNA. Translation: CAB61849.1. BC004689 mRNA. Translation: AAH04689.1.
IPI	IPI00136625.
RefSeq	NP_062415.1. NM_019542.2.
UniGene	Mm.190650.
3D structure databases
ProteinModelPortal	Q9QZ08.
SMR	Q9QZ08. Positions 2-343.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QZ08. 1 interaction.
PTM databases
PhosphoSite	Q9QZ08.
2D gel databases
REPRODUCTION-2DPAGE	Q9QZ08.
Proteomic databases
PaxDb	Q9QZ08.
PRIDE	Q9QZ08.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000037376; ENSMUSP00000042026; ENSMUSG00000034744.
GeneID	56174.
KEGG	mmu:56174.
Organism-specific databases
CTD	55577.
MGI	MGI:1860418. Nagk.
Phylogenomic databases
eggNOG	COG2971.
GeneTree	ENSGT00510000047418.
HOGENOM	HOG000007248.
HOVERGEN	HBG052570.
KO	K00884.
OrthoDB	EOG4GF3FF.
Enzyme and pathway databases
BRENDA	2.7.1.59. 3474.
SABIO-RK	Q9QZ08.
UniPathway	UPA00629.
Gene expression databases
ArrayExpress	Q9QZ08.
Bgee	Q9QZ08.
CleanEx	MM_NAGK.
Genevestigator	Q9QZ08.
GermOnline	ENSMUSG00000034744. Mus musculus.
Family and domain databases
InterPro	IPR002731. ATPase_BadF. [Graphical view]
Pfam	PF01869. BcrAD_BadFG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	311940.
SOURCE	Search...

Entry information

Entry name

NAGK_MOUSE

Accession

Primary (citable) accession number: Q9QZ08

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program