Reviewed, UniProtKB/Swiss-Prot Q9QZ06 (TOLIP_MOUSE)

Names and origin

Protein names

Recommended name:
    Toll-interacting protein

Gene names

Name:

Tollip

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
Subunit structure	Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2 By similarity.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney, thymus, pancreas and testis.
Sequence similarities	Belongs to the tollip family. Contains 1 C2 domain. Contains 1 CUE domain.

Ontologies

Keywords
Biological process	Immune response Inflammatory response
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	immune response Inferred from electronic annotation. Source: UniProtKB-KW inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Il1rap	Q61730	1	EBI-74272,EBI-525035
Il1rl1	P14719	1	EBI-74272,EBI-525078
Irak1	Q62406	2	EBI-74272,EBI-448533
Tom1	O88746	1	EBI-74272,EBI-74264

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 274	273	Toll-interacting protein		PRO_0000072626
Regions
	Domain	40 – 135	96	C2
	Domain	229 – 272	44	CUE
	Compositional bias	28 – 44	17	Gln-rich
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	83	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QZ06-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C3624DB407B0C17B	FASTA	274	30,345
	10 20 30 40 50 60 MATTVSTQRG PVYIGELPQD FLRITPTQQQ QQIQLDAQAA QQLQYGGTVG TVGRLSITVV 70 80 90 100 110 120 QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIQCTVPP GVDSFYLEIF 130 140 150 160 170 180 DERAFSMDDR IAWTHITIPE SLKQGQVEDE WYSLSGRQGD DKEGMINLVM SYTSLPAAMM 190 200 210 220 230 240 MPPQPVVLMP TVYQQGVGYV PIAGMPAVCS PGMVPMAMPP PAVAPQPRCN EEDLKAIQDM 250 260 270 FPNMDREVIR SVLEAQRGNK DAAINSLLQM GEES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tollip, a new component of the IL-1R1 pathway, links IRAK to the IL-1 receptor." Burns K., Clatworthy J., Martin L., Martinon F., Plumpton C., Maschera B., Lewis A., Ray K., Tschopp J., Volpe F. Nat. Cell Biol. 2:346-351(2000) [PubMed: 10854325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Corpora quadrigemina and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ242971 mRNA. Translation: CAB58121.1. AK015062 mRNA. No translation available. AK016482 mRNA. Translation: BAB30262.1. AK045422 mRNA. Translation: BAC32357.1. AK049263 mRNA. Translation: BAC33643.1. AK161312 mRNA. Translation: BAE36316.1. BC062139 mRNA. Translation: AAH62139.1.
IPI	IPI00136618.
RefSeq	NP_076253.1.
UniGene	Mm.103551
3D structure databases
SMR	Q9QZ06. Positions 47-175, 231-273.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QZ06. 4 interactions.
STRING	Q9QZ06.
Proteomic databases
PRIDE	Q9QZ06.
Genome annotation databases
Ensembl	ENSMUST00000001950; ENSMUSP00000001950; ENSMUSG00000025139; Mus musculus. [Genome view]
GeneID	54473.
KEGG	mmu:54473.
UCSC	uc009kmd.1. mouse.
Organism-specific databases
CTD	54473.
MGI	MGI:1891808. Tollip.
Phylogenomic databases
eggNOG	roNOG15441.
HOGENOM	HBG315923.
HOVERGEN	Q9QZ06.
InParanoid	Q9QZ06.
OMA	HVTIPES.
PhylomeDB	Q9QZ06.
Gene expression databases
ArrayExpress	Q9QZ06.
Bgee	Q9QZ06.
CleanEx	MM_TOLLIP.
Genevestigator	Q9QZ06.
GermOnline	ENSMUSG00000025139. Mus musculus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR003892. CUE. IPR009060. UBA-like. [Graphical view]
Pfam	PF00168. C2. 1 hit. PF02845. CUE. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. SM00546. CUE. 1 hit. [Graphical view]
PROSITE	PS50004. C2. False negative. PS51140. CUE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	311336.
SOURCE	Search...

Entry information

Entry name

TOLIP_MOUSE

Accession

Primary (citable) accession number: Q9QZ06
Secondary accession number(s): Q543N1, Q9D5P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)