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Protein

Alcohol dehydrogenase 4

Gene

Adh4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the reduction of benzoquinones.1 Publication

Miscellaneous

Has much lower enzymatic activity towards alcohols and aldehydes compared to human class II ADH. Strongly inhibited by omega-hydroxy fatty acids.

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Zinc 1; catalytic1
Binding sitei49NAD1 Publication1
Metal bindingi68Zinc 1; catalytic1
Metal bindingi98Zinc 21
Metal bindingi101Zinc 21
Metal bindingi104Zinc 21
Metal bindingi112Zinc 21
Metal bindingi179Zinc 1; catalytic1
Binding sitei228NAD1 Publication1
Binding sitei233NAD1 Publication1
Binding sitei372NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 209NAD1 Publication6
Nucleotide bindingi297 – 299NAD1 Publication3
Nucleotide bindingi320 – 322NAD1 Publication3

GO - Molecular functioni

GO - Biological processi

  • alcohol catabolic process Source: MGI
  • alcohol metabolic process Source: MGI
  • cellular aldehyde metabolic process Source: MGI
  • ethanol metabolic process Source: MGI
  • ethanol oxidation Source: MGI
  • quinone metabolic process Source: MGI
  • retinoid metabolic process Source: MGI
  • retinol metabolic process Source: MGI

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BRENDAi1.1.1.1 3474
ReactomeiR-MMU-5365859 RA biosynthesis pathway
R-MMU-71384 Ethanol oxidation
SABIO-RKiQ9QYY9

Chemistry databases

SwissLipidsiSLP:000000496

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 4 (EC:1.1.1.1)
Alternative name(s):
ADH2
Alcohol dehydrogenase class II
Short name:
Alcohol dehydrogenase II
Gene namesi
Name:Adh4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1349472 Adh4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48P → H: Strongly increases enzyme activity, serves as proton acceptor. 1 Publication1
Mutagenesisi52N → H: No effect. 1 Publication1
Mutagenesisi183S → T: Strongly increases activity towards ethanol, increases KM for benzoquinone 10-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001606821 – 377Alcohol dehydrogenase 4Add BLAST377

Proteomic databases

MaxQBiQ9QYY9
PaxDbiQ9QYY9
PRIDEiQ9QYY9

PTM databases

iPTMnetiQ9QYY9
PhosphoSitePlusiQ9QYY9

Expressioni

Tissue specificityi

Liver specific.1 Publication

Gene expression databases

BgeeiENSMUSG00000037797
ExpressionAtlasiQ9QYY9 baseline and differential
GenevisibleiQ9QYY9 MM

Interactioni

Subunit structurei

Dimer.1 Publication

Protein-protein interaction databases

BioGridi205034, 1 interactor
IntActiQ9QYY9, 1 interactor
MINTiQ9QYY9
STRINGi10090.ENSMUSP00000013458

Structurei

Secondary structure

1377
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 15Combined sources8
Beta strandi23 – 29Combined sources7
Beta strandi36 – 45Combined sources10
Helixi48 – 52Combined sources5
Beta strandi62 – 64Combined sources3
Beta strandi69 – 77Combined sources9
Beta strandi89 – 92Combined sources4
Beta strandi99 – 101Combined sources3
Helixi102 – 105Combined sources4
Helixi121 – 123Combined sources3
Beta strandi134 – 137Combined sources4
Beta strandi140 – 143Combined sources4
Turni146 – 148Combined sources3
Beta strandi151 – 158Combined sources8
Helixi159 – 161Combined sources3
Beta strandi162 – 164Combined sources3
Helixi171 – 174Combined sources4
Helixi175 – 178Combined sources4
Helixi180 – 189Combined sources10
Turni190 – 192Combined sources3
Beta strandi199 – 203Combined sources5
Helixi207 – 218Combined sources12
Beta strandi222 – 227Combined sources6
Helixi231 – 233Combined sources3
Helixi234 – 239Combined sources6
Beta strandi243 – 246Combined sources4
Helixi248 – 250Combined sources3
Helixi255 – 262Combined sources8
Beta strandi267 – 274Combined sources8
Helixi277 – 285Combined sources9
Turni289 – 291Combined sources3
Beta strandi293 – 296Combined sources4
Beta strandi300 – 307Combined sources8
Helixi308 – 312Combined sources5
Beta strandi316 – 319Combined sources4
Helixi322 – 324Combined sources3
Helixi327 – 339Combined sources13
Helixi345 – 348Combined sources4
Beta strandi349 – 354Combined sources6
Helixi355 – 357Combined sources3
Helixi358 – 366Combined sources9
Beta strandi371 – 376Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3EX-ray2.12A/B2-377[»]
1E3IX-ray2.08A/B2-377[»]
1E3LX-ray2.50A/B2-377[»]
ProteinModelPortaliQ9QYY9
SMRiQ9QYY9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QYY9

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0022 Eukaryota
COG1062 LUCA
GeneTreeiENSGT00430000030800
HOGENOMiHOG000294674
HOVERGENiHBG000195
InParanoidiQ9QYY9
KOiK13980
OMAiMKAALDC
OrthoDBiEOG091G08N3
TreeFamiTF300429

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR028632 Zinc_ADH_II
PANTHERiPTHR43880:SF14 PTHR43880:SF14, 1 hit
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QYY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTQGKVIKC KAAIAWKTGS PLCIEEIEVS PPKACEVRIQ VIATCVCPTD
60 70 80 90 100
INATDPKKKA LFPVVLGHEC AGIVESVGPG VTNFKPGDKV IPFFAPQCKR
110 120 130 140 150
CKLCLSPLTN LCGKLRNFKY PTIDQELMED RTSRFTCKGR SIYHFMGVSS
160 170 180 190 200
FSQYTVVSEA NLARVDDEAN LERVCLIGCG FSSGYGAAIN TAKVTPSSTC
210 220 230 240 250
AVFGLGCVGL SAIIGCKIAG ASRIIAIDIN GEKFPKAKAL GATDCLNPRE
260 270 280 290 300
LDKPVQDVIT ELTAGGVDYS LDCAGTAQTL KAAVDCTVLG WGSCTVVGAK
310 320 330 340 350
VDKMTIPTVD VILGRSINGT FFGGWKSVDS VPNLVSDYKN KKFDLDLLVT
360 370
HALPFESIND AIDLMKEGKS IRTILTF
Length:377
Mass (Da):40,211
Last modified:June 28, 2011 - v4
Checksum:i5B527E48BB745E14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197S → G in CAB57455 (PubMed:10514444).Curated1
Sequence conflicti303K → E in CAB57455 (PubMed:10514444).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245750 mRNA Translation: CAB57455.1
AK132994 mRNA Translation: BAE21459.1
CCDSiCCDS38653.1
RefSeqiNP_036126.2, NM_011996.2
UniGeneiMm.158750

Genome annotation databases

EnsembliENSMUST00000013458; ENSMUSP00000013458; ENSMUSG00000037797
GeneIDi26876
KEGGimmu:26876
UCSCiuc008rnj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiADH4_MOUSE
AccessioniPrimary (citable) accession number: Q9QYY9
Secondary accession number(s): Q3V0P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: June 28, 2011
Last modified: February 28, 2018
This is version 139 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health