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Protein

Alcohol dehydrogenase 4

Gene

Adh4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the reduction of benzoquinones.1 Publication

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Zinc 1; catalytic1
Binding sitei49NAD1 Publication1
Metal bindingi68Zinc 1; catalytic1
Metal bindingi98Zinc 21
Metal bindingi101Zinc 21
Metal bindingi104Zinc 21
Metal bindingi112Zinc 21
Metal bindingi179Zinc 1; catalytic1
Binding sitei228NAD1 Publication1
Binding sitei233NAD1 Publication1
Binding sitei372NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 209NAD1 Publication6
Nucleotide bindingi297 – 299NAD1 Publication3
Nucleotide bindingi320 – 322NAD1 Publication3

GO - Molecular functioni

GO - Biological processi

  • alcohol catabolic process Source: MGI
  • alcohol metabolic process Source: MGI
  • cellular aldehyde metabolic process Source: MGI
  • ethanol metabolic process Source: MGI
  • ethanol oxidation Source: MGI
  • quinone metabolic process Source: MGI
  • retinoid metabolic process Source: MGI
  • retinol metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BRENDAi1.1.1.1. 3474.
ReactomeiR-MMU-5365859. RA biosynthesis pathway.
R-MMU-71384. Ethanol oxidation.
SABIO-RKQ9QYY9.

Chemistry databases

SwissLipidsiSLP:000000496.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 4 (EC:1.1.1.1)
Alternative name(s):
ADH2
Alcohol dehydrogenase class II
Short name:
Alcohol dehydrogenase II
Gene namesi
Name:Adh4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1349472. Adh4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48P → H: Strongly increases enzyme activity, serves as proton acceptor. 1 Publication1
Mutagenesisi52N → H: No effect. 1 Publication1
Mutagenesisi183S → T: Strongly increases activity towards ethanol, increases KM for benzoquinone 10-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001606821 – 377Alcohol dehydrogenase 4Add BLAST377

Proteomic databases

MaxQBiQ9QYY9.
PaxDbiQ9QYY9.
PRIDEiQ9QYY9.

PTM databases

iPTMnetiQ9QYY9.
PhosphoSitePlusiQ9QYY9.

Expressioni

Tissue specificityi

Liver specific.1 Publication

Gene expression databases

BgeeiENSMUSG00000037797.
ExpressionAtlasiQ9QYY9. baseline and differential.
GenevisibleiQ9QYY9. MM.

Interactioni

Subunit structurei

Dimer.1 Publication

Protein-protein interaction databases

BioGridi205034. 1 interactor.
IntActiQ9QYY9. 1 interactor.
MINTiMINT-4087269.
STRINGi10090.ENSMUSP00000013458.

Structurei

Secondary structure

1377
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 15Combined sources8
Beta strandi23 – 29Combined sources7
Beta strandi36 – 45Combined sources10
Helixi48 – 52Combined sources5
Beta strandi62 – 64Combined sources3
Beta strandi69 – 77Combined sources9
Beta strandi89 – 92Combined sources4
Beta strandi99 – 101Combined sources3
Helixi102 – 105Combined sources4
Helixi121 – 123Combined sources3
Beta strandi134 – 137Combined sources4
Beta strandi140 – 143Combined sources4
Turni146 – 148Combined sources3
Beta strandi151 – 158Combined sources8
Helixi159 – 161Combined sources3
Beta strandi162 – 164Combined sources3
Helixi171 – 174Combined sources4
Helixi175 – 178Combined sources4
Helixi180 – 189Combined sources10
Turni190 – 192Combined sources3
Beta strandi199 – 203Combined sources5
Helixi207 – 218Combined sources12
Beta strandi222 – 227Combined sources6
Helixi231 – 233Combined sources3
Helixi234 – 239Combined sources6
Beta strandi243 – 246Combined sources4
Helixi248 – 250Combined sources3
Helixi255 – 262Combined sources8
Beta strandi267 – 274Combined sources8
Helixi277 – 285Combined sources9
Turni289 – 291Combined sources3
Beta strandi293 – 296Combined sources4
Beta strandi300 – 307Combined sources8
Helixi308 – 312Combined sources5
Beta strandi316 – 319Combined sources4
Helixi322 – 324Combined sources3
Helixi327 – 339Combined sources13
Helixi345 – 348Combined sources4
Beta strandi349 – 354Combined sources6
Helixi355 – 357Combined sources3
Helixi358 – 366Combined sources9
Beta strandi371 – 376Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3EX-ray2.12A/B2-377[»]
1E3IX-ray2.08A/B2-377[»]
1E3LX-ray2.50A/B2-377[»]
ProteinModelPortaliQ9QYY9.
SMRiQ9QYY9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QYY9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0022. Eukaryota.
COG1062. LUCA.
GeneTreeiENSGT00430000030800.
HOGENOMiHOG000294674.
HOVERGENiHBG000195.
InParanoidiQ9QYY9.
KOiK13980.
OMAiDSKFEGL.
OrthoDBiEOG091G08N3.
TreeFamiTF300429.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR028632. Zinc_ADH_II.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 2 hits.
PTHR11695:SF308. PTHR11695:SF308. 2 hits.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QYY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTQGKVIKC KAAIAWKTGS PLCIEEIEVS PPKACEVRIQ VIATCVCPTD
60 70 80 90 100
INATDPKKKA LFPVVLGHEC AGIVESVGPG VTNFKPGDKV IPFFAPQCKR
110 120 130 140 150
CKLCLSPLTN LCGKLRNFKY PTIDQELMED RTSRFTCKGR SIYHFMGVSS
160 170 180 190 200
FSQYTVVSEA NLARVDDEAN LERVCLIGCG FSSGYGAAIN TAKVTPSSTC
210 220 230 240 250
AVFGLGCVGL SAIIGCKIAG ASRIIAIDIN GEKFPKAKAL GATDCLNPRE
260 270 280 290 300
LDKPVQDVIT ELTAGGVDYS LDCAGTAQTL KAAVDCTVLG WGSCTVVGAK
310 320 330 340 350
VDKMTIPTVD VILGRSINGT FFGGWKSVDS VPNLVSDYKN KKFDLDLLVT
360 370
HALPFESIND AIDLMKEGKS IRTILTF
Length:377
Mass (Da):40,211
Last modified:June 28, 2011 - v4
Checksum:i5B527E48BB745E14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197S → G in CAB57455 (PubMed:10514444).Curated1
Sequence conflicti303K → E in CAB57455 (PubMed:10514444).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245750 mRNA. Translation: CAB57455.1.
AK132994 mRNA. Translation: BAE21459.1.
CCDSiCCDS38653.1.
RefSeqiNP_036126.2. NM_011996.2.
UniGeneiMm.158750.

Genome annotation databases

EnsembliENSMUST00000013458; ENSMUSP00000013458; ENSMUSG00000037797.
GeneIDi26876.
KEGGimmu:26876.
UCSCiuc008rnj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245750 mRNA. Translation: CAB57455.1.
AK132994 mRNA. Translation: BAE21459.1.
CCDSiCCDS38653.1.
RefSeqiNP_036126.2. NM_011996.2.
UniGeneiMm.158750.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3EX-ray2.12A/B2-377[»]
1E3IX-ray2.08A/B2-377[»]
1E3LX-ray2.50A/B2-377[»]
ProteinModelPortaliQ9QYY9.
SMRiQ9QYY9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205034. 1 interactor.
IntActiQ9QYY9. 1 interactor.
MINTiMINT-4087269.
STRINGi10090.ENSMUSP00000013458.

Chemistry databases

SwissLipidsiSLP:000000496.

PTM databases

iPTMnetiQ9QYY9.
PhosphoSitePlusiQ9QYY9.

Proteomic databases

MaxQBiQ9QYY9.
PaxDbiQ9QYY9.
PRIDEiQ9QYY9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000013458; ENSMUSP00000013458; ENSMUSG00000037797.
GeneIDi26876.
KEGGimmu:26876.
UCSCiuc008rnj.1. mouse.

Organism-specific databases

CTDi127.
MGIiMGI:1349472. Adh4.

Phylogenomic databases

eggNOGiKOG0022. Eukaryota.
COG1062. LUCA.
GeneTreeiENSGT00430000030800.
HOGENOMiHOG000294674.
HOVERGENiHBG000195.
InParanoidiQ9QYY9.
KOiK13980.
OMAiDSKFEGL.
OrthoDBiEOG091G08N3.
TreeFamiTF300429.

Enzyme and pathway databases

BRENDAi1.1.1.1. 3474.
ReactomeiR-MMU-5365859. RA biosynthesis pathway.
R-MMU-71384. Ethanol oxidation.
SABIO-RKQ9QYY9.

Miscellaneous databases

EvolutionaryTraceiQ9QYY9.
PROiQ9QYY9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037797.
ExpressionAtlasiQ9QYY9. baseline and differential.
GenevisibleiQ9QYY9. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR028632. Zinc_ADH_II.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 2 hits.
PTHR11695:SF308. PTHR11695:SF308. 2 hits.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADH4_MOUSE
AccessioniPrimary (citable) accession number: Q9QYY9
Secondary accession number(s): Q3V0P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: June 28, 2011
Last modified: November 2, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Has much lower enzymatic activity towards alcohols and aldehydes compared to human class II ADH. Strongly inhibited by omega-hydroxy fatty acids.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.